FastQCFastQC Report
Thu 26 May 2016
SRR1512871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241904
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61090.27249159642874987No Hit
GTCCTACAGTGGACATTTCTAAATT54260.24202642039980304No Hit
GTCCTAAAGTGTGTATTTCTCATTT48380.21579871395028513No Hit
CTGTAGGACGTGGAATATGGCAAGA44270.1974660824013874No Hit
CTTTAGGACGTGAAATATGGCGAGG41560.18537814286427964No Hit
TATCAACGCAGAGTACTTTTTTTTT35710.1592842512435858No Hit
GGTATCAACGCAGAGTACTTTTTTT34400.15344100371826805No Hit
GTCCTACAGTGTGCATTTCTCATTT29240.13042485316052782No Hit
GTACTGGTTCACTATCGGTCAGTCA25340.1130289254133986No Hit
CTGTAGGACCTGGAATATGGCGAGA23410.10442017142571672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC801.2922828E-714.24728810
GGTATCA17950.014.1169171
TAGGACC8650.013.2870814
CGGTAGC907.2386138E-611.6432121
TACACCG752.0774029E-411.3983395
TAGAAAT7800.011.3252724
AATGTCC7500.011.145548
AGTCGTG600.005881452511.0822137
GGACCTG16450.011.0292816
AGGACCT18100.010.9680155
GTCCTAA14900.010.9328281
GTCCTAC28200.010.8775081
ATTTAGA8000.010.8361261
TTTAGAA9150.010.796242
AAATGTC7050.010.7790527
TCTACAC3100.010.7242443
ATCGCGC2050.010.6574588
CCTATAC1355.7027137E-810.5540173
GTATCAA38700.010.5108931
GAAATGT8050.010.3840446
Produced by FastQC (version 0.11.5)