##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512871_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2241904
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.712013092442852	31.0	30.0	34.0	16.0	34.0
2	28.784117428756986	31.0	30.0	34.0	16.0	34.0
3	28.881164403114497	31.0	30.0	34.0	16.0	34.0
4	32.250755607733424	35.0	33.0	37.0	20.0	37.0
5	32.41365508960241	35.0	33.0	37.0	23.0	37.0
6	32.31652291980388	36.0	33.0	37.0	22.0	37.0
7	32.24450823942506	36.0	33.0	37.0	20.0	37.0
8	32.1692507796944	36.0	33.0	37.0	19.0	37.0
9	33.45379686195305	38.0	34.0	39.0	18.0	39.0
10	33.28523255233052	38.0	33.0	39.0	17.0	39.0
11	33.38245214781721	38.0	34.0	39.0	17.0	39.0
12	33.2710031294828	38.0	33.0	39.0	17.0	39.0
13	33.253159368108534	38.0	33.0	39.0	17.0	39.0
14	34.103093174373214	39.0	33.0	41.0	16.0	41.0
15	34.29032777496271	39.0	34.0	41.0	17.0	41.0
16	34.24351622549404	39.0	34.0	41.0	17.0	41.0
17	34.01187740420642	39.0	33.0	41.0	11.0	41.0
18	34.01780986161763	39.0	33.0	41.0	11.0	41.0
19	34.1251213254448	39.0	34.0	41.0	11.0	41.0
20	34.087909651795975	39.0	34.0	41.0	10.0	41.0
21	33.97995052419729	39.0	34.0	41.0	10.0	41.0
22	33.99603060612765	39.0	34.0	41.0	7.0	41.0
23	33.67647142785775	39.0	34.0	41.0	2.0	41.0
24	33.736473997102465	39.0	34.0	41.0	2.0	41.0
25	33.46102732320385	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.796899744611494
1101	2	0.5765853757953643
1101	3	0.7809126549455172
1101	4	0.8663643468944002
1101	5	0.8118451216413831
1101	6	0.7987641226597546
1101	7	0.6486837743904985
1101	8	0.90475947322048
1101	9	1.0080474360478249
1101	10	0.97521098845246
1101	11	1.0222008709712682
1101	12	0.8514560952112689
1101	13	1.2000195313710265
1101	14	1.3085387511959823
1101	15	1.1127104625845732
1101	16	0.9905121376149921
1101	17	0.44659597435786225
1101	18	0.9103187057152482
1101	19	0.3198809888054157
1101	20	-0.26080253370031414
1101	21	0.8217745953036584
1101	22	0.8796331930621335
1101	23	0.4992582947892288
1101	24	0.9996446517390751
1101	25	0.687886269620158
1102	1	1.0962177326762834
1102	2	1.0570968847467732
1102	3	1.0538367811177238
1102	4	1.1736021985568001
1102	5	1.0741170739005454
1102	6	1.0961469018583898
1102	7	0.9748346695311625
1102	8	1.1292607519928595
1102	9	1.3202852877102345
1102	10	1.2570865212742817
1102	11	1.088232414023274
1102	12	1.2356206305906383
1102	13	1.241754399231219
1102	14	1.4986410529862582
1102	15	1.4449994651421179
1102	16	1.3167141836508023
1102	17	1.4125721039401355
1102	18	1.4315335394748345
1102	19	1.1823319691975769
1102	20	1.5047388132732564
1102	21	1.568377323351399
1102	22	1.4144967906750878
1102	23	1.4113725317883734
1102	24	0.7288219748422264
1102	25	1.177336397497399
1103	1	0.47756426402156293
1103	2	0.5324558623185034
1103	3	0.6551557469950176
1103	4	0.7424331721190143
1103	5	0.6818086747859837
1103	6	0.2631326596999344
1103	7	0.0630090837649675
1103	8	0.39108257883160036
1103	9	0.5720557425404564
1103	10	0.7226054620920621
1103	11	0.3732476585623843
1103	12	0.4676274801917799
1103	13	0.6954602840409763
1103	14	0.8543007447542124
1103	15	0.7860695064903211
1103	16	0.7533812679760743
1103	17	0.40730626423750493
1103	18	0.8167935319569821
1103	19	0.7013905666999776
1103	20	1.0051238945078538
1103	21	0.7584002945747272
1103	22	0.7078150882986165
1103	23	0.25905995608775356
1103	24	0.36596351934375093
1103	25	0.6714724706711834
1104	1	1.0184237554134192
1104	2	0.8928432801547146
1104	3	0.9453377543265873
1104	4	1.1301901406849666
1104	5	0.9385214328844427
1104	6	0.8156951345854822
1104	7	0.9481739536957576
1104	8	0.7737965251670076
1104	9	1.0423365888346936
1104	10	1.0325782706946995
1104	11	0.8014797082546821
1104	12	1.0895361535819248
1104	13	1.026049907593375
1104	14	1.29510427560934
1104	15	1.1135800679117338
1104	16	1.112356935414354
1104	17	1.3639332326144569
1104	18	1.12555369791572
1104	19	0.7159162292488972
1104	20	0.8114848243566755
1104	21	0.918457783549016
1104	22	0.6428034072555917
1104	23	1.0448640580526174
1104	24	0.971482740267902
1104	25	0.47953448579285407
1105	1	0.5207850662479458
1105	2	0.5990470579448335
1105	3	0.6586200883583508
1105	4	0.7825042091335987
1105	5	0.5779889032254673
1105	6	0.8038010645700595
1105	7	0.6871868348151224
1105	8	0.5789088085996568
1105	9	0.1923798131721952
1105	10	0.5505371279811371
1105	11	0.5663614640432044
1105	12	0.731153805313177
1105	13	0.8263542767803926
1105	14	0.8435051448983444
1105	15	0.8592963796278852
1105	16	0.8260858629939989
1105	17	1.0814552948935656
1105	18	0.9270767403722644
1105	19	0.6749356928531824
1105	20	0.7894267896706566
1105	21	0.6727470097868888
1105	22	0.6717935859973068
1105	23	0.8605163211072124
1105	24	0.9695059736423772
1105	25	1.1369226945105027
1106	1	0.7552901626223374
1106	2	0.8473987466512938
1106	3	0.7429457388908816
1106	4	0.7975730777613066
1106	5	0.6354476338247892
1106	6	0.7059238453586403
1106	7	0.5756042905549563
1106	8	0.722325538152873
1106	9	0.7928325963196343
1106	10	0.5796402560754927
1106	11	0.48726882347315836
1106	12	0.6988440422220492
1106	13	0.646539882802152
1106	14	0.9925287299179217
1106	15	0.7165676250528321
1106	16	0.769118424937389
1106	17	0.9796957066661918
1106	18	0.8729170309263097
1106	19	0.8720791492831452
1106	20	1.0604068835555012
1106	21	1.0682846509011625
1106	22	0.9658398647623159
1106	23	1.0979761203091698
1106	24	0.817220669310629
1106	25	1.011520355693328
1107	1	0.9427206767055196
1107	2	0.9311297017179037
1107	3	0.901630429419928
1107	4	1.064304298215113
1107	5	0.776896414582886
1107	6	0.9672733602898305
1107	7	0.7911460806126271
1107	8	0.952820632702327
1107	9	0.9404069930336334
1107	10	1.1114597428834188
1107	11	1.0327122485297906
1107	12	1.094265853938424
1107	13	0.9105168605442984
1107	14	1.248835564172765
1107	15	1.0327871569668545
1107	16	1.0447682266098823
1107	17	1.368013064381877
1107	18	1.321268033516347
1107	19	1.1447609241175059
1107	20	1.3455992827885694
1107	21	1.1245608311531825
1107	22	1.1287982125828577
1107	23	1.3729079345951618
1107	24	1.214562811727312
1107	25	1.5082927743492363
1108	1	0.792255764615728
1108	2	0.8488830935531979
1108	3	0.8025748961366475
1108	4	0.8895645358594919
1108	5	0.8265549950673545
1108	6	0.8837809829070409
1108	7	0.9190384911872158
1108	8	0.8675326988219965
1108	9	0.8972390401333854
1108	10	0.9954618558999684
1108	11	0.6990253614985775
1108	12	0.8318580532805697
1108	13	0.9397309523559301
1108	14	0.9410448192559926
1108	15	0.8967801353165896
1108	16	0.64663387348741
1108	17	0.9554706546647722
1108	18	1.109561786497821
1108	19	0.9571238089464913
1108	20	1.0497697373016877
1108	21	0.9825303742145053
1108	22	1.0345761777331575
1108	23	1.3448332536567449
1108	24	1.1659843490691273
1108	25	1.1921799311445866
1109	1	0.6652406405771707
1109	2	0.6579664499641638
1109	3	0.866197697433762
1109	4	0.896210894208977
1109	5	0.6159029551534729
1109	6	0.7689472758649387
1109	7	0.7172750622380377
1109	8	0.6977039497488207
1109	9	0.6610146004732016
1109	10	0.8278485596099543
1109	11	0.8142539193996186
1109	12	0.7463826689263087
1109	13	0.8065697334995647
1109	14	0.9737602675725157
1109	15	0.7638493950158889
1109	16	0.4398838891206438
1109	17	0.8269994064179258
1109	18	0.895039850835289
1109	19	0.8390687620914505
1109	20	0.750161908177418
1109	21	0.7697611475683246
1109	22	0.7794149767433467
1109	23	1.035841910002496
1109	24	0.9584339430516735
1109	25	0.6940264395731361
1110	1	0.5194695318111044
1110	2	0.48497440403389547
1110	3	0.45812440346886163
1110	4	0.45958629041734156
1110	5	0.35001285365853363
1110	6	0.5429351720974154
1110	7	0.4509497344196305
1110	8	0.37006572777182356
1110	9	0.5252239875487561
1110	10	0.5488318315627438
1110	11	0.45213249974382563
1110	12	0.4593042220823804
1110	13	0.5489963903402
1110	14	0.5848272858670711
1110	15	0.4309680076934015
1110	16	0.5044505630539433
1110	17	0.6162804148465781
1110	18	-0.04483988316734866
1110	19	0.43205228098116066
1110	20	0.43846925204286435
1110	21	0.5526310561823706
1110	22	0.6503383472008153
1110	23	0.6504088733150013
1110	24	0.5111192856091265
1110	25	0.6672353269872602
1111	1	0.4145762971266507
1111	2	0.5210159836931219
1111	3	0.515685717373831
1111	4	0.4866674765027881
1111	5	0.5991390754346
1111	6	0.3797357759450293
1111	7	0.37003147224401545
1111	8	0.5673254243920383
1111	9	0.6844926269375549
1111	10	0.5443999119327145
1111	11	0.4874024111973867
1111	12	0.12153884140167293
1111	13	0.28005967556241274
1111	14	0.4412521708256705
1111	15	0.4719051209493159
1111	16	0.49293799375131897
1111	17	0.08780685425299595
1111	18	0.42483861395709255
1111	19	0.443037633078724
1111	20	0.6087370370026193
1111	21	0.5429338612384456
1111	22	0.29212950635067614
1111	23	0.5563105641649457
1111	24	0.6255261854110401
1111	25	0.6655161237902405
1112	1	0.426935646296112
1112	2	0.3895707389547134
1112	3	0.26882759556965397
1112	4	0.4283002761956993
1112	5	0.4476045803544437
1112	6	0.26802399562302526
1112	7	0.09151586497321063
1112	8	0.24336206918844994
1112	9	0.22630620434387794
1112	10	0.28827920165323206
1112	11	0.12525941833387577
1112	12	0.240051787009115
1112	13	0.3109543560051051
1112	14	0.26654758853373295
1112	15	-0.10177310549054397
1112	16	0.28577173441145476
1112	17	0.43943496911069246
1112	18	0.2782018871568397
1112	19	0.42918090595537706
1112	20	0.08034632020744681
1112	21	0.23537442960358135
1112	22	-0.04863109857001291
1112	23	0.4193715231791728
1112	24	0.31540963378823506
1112	25	0.268036780528746
1113	1	-0.10745972698779838
1113	2	-0.0014256629396562914
1113	3	0.04110226925277871
1113	4	0.18251322064508457
1113	5	-0.04960375376676751
1113	6	-0.32634369561548127
1113	7	-0.28382190243644345
1113	8	-0.03842518525829064
1113	9	-0.14097556960413726
1113	10	-0.13533779910990518
1113	11	-0.2556773217259263
1113	12	-0.5204189151479
1113	13	-0.377778108394935
1113	14	-0.18823167295001042
1113	15	-0.49856504169997606
1113	16	-0.5616424875486814
1113	17	-0.3135334738711322
1113	18	-0.6302533692685941
1113	19	-0.9008013077818546
1113	20	-0.5935242614446707
1113	21	-0.557734379884046
1113	22	-0.9350434349855377
1113	23	-4.41943810684835
1113	24	-2.051718532587909
1113	25	-1.2039320553429604
1114	1	-1.033754780341095
1114	2	-1.291210834759724
1114	3	-1.0456554884314961
1114	4	-0.8133699819228077
1114	5	-1.0872949067196558
1114	6	-1.4372118558815288
1114	7	-1.414821974699727
1114	8	-1.231785605455773
1114	9	-1.7318858471696146
1114	10	-1.3116827943293146
1114	11	-1.4458515286118576
1114	12	-2.2548011872544294
1114	13	-1.5336234099762898
1114	14	-3.523679218768656
1114	15	-2.3458601239422237
1114	16	-2.254657345223162
1114	17	-2.1085155050291284
1114	18	-1.9535371142775801
1114	19	-2.062787597072152
1114	20	-1.5896317011500471
1114	21	-1.5737321447144481
1114	22	-1.6404364281642287
1114	23	-5.920740850962595
1114	24	-3.1723712885318918
1114	25	-3.0555065478611745
1115	1	-1.9788855337956264
1115	2	-1.84908761367031
1115	3	-1.5291162855033065
1115	4	-1.5431921200556076
1115	5	-1.5451714488327433
1115	6	-1.758068138498853
1115	7	-2.1934493020618966
1115	8	-2.5185628903046293
1115	9	-3.7477938214560176
1115	10	-4.682461868714022
1115	11	-3.279812143962001
1115	12	-3.526302012248749
1115	13	-2.806200928699422
1115	14	-3.515543836576267
1115	15	-3.150307033983914
1115	16	-3.2904249541179595
1115	17	-3.1733873228688125
1115	18	-5.578570725232844
1115	19	-3.7550834557860284
1115	20	-3.9935465843709075
1115	21	-3.6179646925959226
1115	22	-3.193260438385387
1115	23	-3.864465762165576
1115	24	-3.500243472754466
1115	25	-3.732717262768336
1116	1	-1.9966977711794662
1116	2	-1.744425245609765
1116	3	-2.1594642108577915
1116	4	-1.887193294518493
1116	5	-1.9423799745158554
1116	6	-4.390102456965494
1116	7	-2.929064028990002
1116	8	-3.282324756992299
1116	9	-4.202189869432253
1116	10	-3.6458031616586943
1116	11	-3.184358111349912
1116	12	-3.007741653474284
1116	13	-4.125097621668953
1116	14	-7.232374830793503
1116	15	-5.172269776661693
1116	16	-4.036483698511898
1116	17	-5.033744545773402
1116	18	-3.8410947174824663
1116	19	-3.6223990845916703
1116	20	-4.406284025637223
1116	21	-4.998929910853931
1116	22	-4.584460635231032
1116	23	-3.6273668507698957
1116	24	-4.593346808792347
1116	25	-4.56363019179344
1201	1	0.8597964502845699
1201	2	0.8891578806348335
1201	3	1.3135252707003886
1201	4	1.2137452785140113
1201	5	0.7704835304588116
1201	6	1.2153594035233652
1201	7	1.2139493784085644
1201	8	1.2268271037117557
1201	9	1.750206013679886
1201	10	1.716025065639812
1201	11	1.7003399860895954
1201	12	1.543433999328748
1201	13	1.5853998874861972
1201	14	1.6601187724722024
1201	15	1.7710518762327467
1201	16	1.6402973698223136
1201	17	1.9414333507552044
1201	18	1.737261120392354
1201	19	2.004919181372692
1201	20	1.9254045432603206
1201	21	1.9896308412707668
1201	22	1.8566889646740705
1201	23	2.15043860207372
1201	24	1.9639561391564584
1201	25	2.075213400294544
1202	1	1.0995455130680405
1202	2	0.9847312044690391
1202	3	1.1054764248816866
1202	4	1.341090531021166
1202	5	1.1701775373804182
1202	6	1.3437974141278488
1202	7	1.3051224475491452
1202	8	1.3002146286666942
1202	9	1.5948692621751803
1202	10	1.5002881523978857
1202	11	1.469614545420285
1202	12	1.6501866321759877
1202	13	1.6161161712554133
1202	14	1.962631795257714
1202	15	1.6826288253669475
1202	16	1.4640338932022843
1202	17	1.7731518988627926
1202	18	1.7770085703127378
1202	19	1.8284546171453187
1202	20	1.6331619898101692
1202	21	1.8586556062631772
1202	22	1.861904669069908
1202	23	1.983868345887764
1202	24	1.9213151608318597
1202	25	2.217154803591221
1203	1	0.9589228214026875
1203	2	1.1039284587586593
1203	3	1.0120128315361185
1203	4	1.1475889941418131
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2211	19	-0.15112747476917576
2211	20	-0.14351121476517648
2211	21	-0.05951306507433429
2211	22	-0.11372382816423965
2211	23	0.06801539694221503
2211	24	-0.3006368211252237
2211	25	0.16097690742300585
2212	1	0.0834790808337047
2212	2	0.1144648730642146
2212	3	-0.15098055619160533
2212	4	0.08527935743714465
2212	5	-0.11845762943364235
2212	6	-0.20956896163238525
2212	7	-0.11428803414663236
2212	8	-0.14549903153620392
2212	9	0.017037241961723737
2212	10	-0.28779610092011865
2212	11	-0.26961023958642016
2212	12	0.03347457406767518
2212	13	0.09644570726052137
2212	14	0.18248157133842824
2212	15	-0.23193091809979194
2212	16	-0.16876655608094637
2212	17	0.004311602522761859
2212	18	0.13558733814705448
2212	19	-0.0819299557248101
2212	20	-0.0011140012170471891
2212	21	0.07264366604795924
2212	22	0.10654798776917573
2212	23	0.15055131665504717
2212	24	0.18792369409268161
2212	25	0.25794114169600135
2213	1	-0.16300525463585913
2213	2	-0.08714240021694408
2213	3	-0.18719753254716665
2213	4	-0.05424094839403182
2213	5	0.07093567341512141
2213	6	0.20353272558002544
2213	7	-0.10947483338016184
2213	8	0.10641395815918031
2213	9	-0.3033838472986048
2213	10	-0.05700931963096423
2213	11	-0.17964938576491818
2213	12	-0.02405704265689934
2213	13	-0.23316625531214186
2213	14	-0.3062306879985215
2213	15	-0.14514307234659896
2213	16	-0.08137039930651468
2213	17	-0.05137806769000264
2213	18	-0.09324474241066127
2213	19	-0.2571486487533292
2213	20	-0.1020492513239617
2213	21	-0.24441140881080514
2213	22	-0.5207950629715867
2213	23	-0.24575215863865907
2213	24	0.014989698486807868
2213	25	0.1738205551910852
2214	1	-2.848889237648077
2214	2	-1.5565764950135659
2214	3	-1.6670340388835285
2214	4	-3.5225355767114053
2214	5	-1.8202502013362931
2214	6	-1.5308861616964116
2214	7	-1.3755336095327664
2214	8	-1.3140606608937695
2214	9	-1.0602392119447046
2214	10	-1.0820150534794664
2214	11	-1.1342056310234696
2214	12	-1.101883604304895
2214	13	-1.4601880061894512
2214	14	-1.3137386446108295
2214	15	-1.730719212315691
2214	16	-1.5216909860587222
2214	17	-3.566707650122229
2214	18	-3.3222502612654843
2214	19	-2.2746377277721237
2214	20	-2.005145119219371
2214	21	-2.0347872415522943
2214	22	-2.9794827563479345
2214	23	-1.7586544460646891
2214	24	-2.7960955231187476
2214	25	-2.836303103098622
2215	1	-2.1010827277597848
2215	2	-3.7217950847674715
2215	3	-2.3225699940477362
2215	4	-3.9328226873702654
2215	5	-2.6035720426507005
2215	6	-2.433096631936621
2215	7	-2.417925335939362
2215	8	-1.9787958431010715
2215	9	-4.520460444656642
2215	10	-2.746066408980635
2215	11	-2.364196292984392
2215	12	-2.2803502429945866
2215	13	-2.3884015522468616
2215	14	-2.116993217382131
2215	15	-2.1904149894835854
2215	16	-2.2773087676988055
2215	17	-2.6115743901562567
2215	18	-2.4024662864629214
2215	19	-2.5759713383994125
2215	20	-2.4424759007880716
2215	21	-3.092748693565788
2215	22	-2.8552303874859
2215	23	-2.8361490291354805
2215	24	-2.735984795350028
2215	25	-3.762331143624806
2216	1	-3.359951633620959
2216	2	-3.3910247407150074
2216	3	-4.769283423485856
2216	4	-5.186483052935099
2216	5	-4.93216880276896
2216	6	-3.8927931379256364
2216	7	-5.262425915862412
2216	8	-3.2813388501668435
2216	9	-4.1071031749212
2216	10	-3.1546042999817523
2216	11	-3.321270285607305
2216	12	-3.733200057104728
2216	13	-3.2854578769836174
2216	14	-4.203240163268358
2216	15	-3.771249469214233
2216	16	-4.037119291768356
2216	17	-3.775987145846738
2216	18	-3.3685687623154443
2216	19	-4.122652401362245
2216	20	-4.568645670955217
2216	21	-4.2291901674130195
2216	22	-3.807793632740651
2216	23	-4.014674640703809
2216	24	-4.3602544673288435
2216	25	-4.32496539278393
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129098.0
3	1.0
4	1132.0
5	6873.0
6	7871.0
7	7223.0
8	6273.0
9	5644.0
10	5225.0
11	4789.0
12	4481.0
13	4444.0
14	4328.0
15	4613.0
16	4637.0
17	5074.0
18	5738.0
19	6840.0
20	8803.0
21	11206.0
22	13937.0
23	16680.0
24	19467.0
25	21836.0
26	24230.0
27	26707.0
28	30227.0
29	35544.0
30	43133.0
31	53355.0
32	66636.0
33	84839.0
34	109352.0
35	144289.0
36	195682.0
37	299669.0
38	822028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68373340303002	18.47605041791157	12.346337612399326	24.493878566659088
2	14.942799979479812	19.800864970479928	42.040018825040875	23.216316224999385
3	19.391704353737733	25.631237994634443	28.431783650487617	26.5452740011402
4	12.325197129994272	16.120674426364655	37.867070413638416	33.68705803000266
5	13.108956915088196	38.981897020904526	34.569820040863306	13.339326023143977
6	32.15997807296542	35.992521952702425	18.39438039387418	13.453119580457976
7	29.036566502913846	31.534641421502435	21.4052982704399	18.02349380514382
8	26.031949155683037	36.565427811223415	20.306962651270013	17.09566038182353
9	28.037198771112028	14.656390815660552	19.724750164503494	37.58166024872393
10	16.538387703077355	28.543736132255315	33.07025728712315	21.847618877544186
11	34.914864713442675	22.199013135132986	24.384213912089294	18.501908239335044
12	24.673292930447445	24.470906439667573	30.72104596267302	20.13475466721196
13	29.65221315855378	22.01721457145038	25.501957743454582	22.82861452654125
14	21.46157860596637	22.343815081234773	27.128079646819693	29.066526665979165
15	23.410502293908696	31.352778917378664	22.91975716754395	22.31696162116869
16	22.248734941507088	27.644100597540643	27.877313321562582	22.22985113938969
17	21.76105262217936	28.647342995169083	27.475108717976195	22.116495664675366
18	21.696494468651675	26.811267100030406	30.041045877351724	21.451192553966198
19	23.848198312630007	26.328400936326496	27.483004191860317	22.340396559183183
20	24.687901535736067	26.477272016994085	27.844469108926628	20.99035733834322
21	23.902154198604816	26.20955771901451	26.579222610045726	23.30906547233495
22	24.149187250690055	27.91503436648596	26.640344477474926	21.29543390534906
23	22.667028599543002	27.586830405576144	27.80305255854696	21.943088436333895
24	23.703601517377653	26.27458963881251	28.20412542071871	21.817683423091125
25	22.872992750261947	27.051224071326814	28.080357401951744	21.99542577645949
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	266.0
2	628.0
3	990.0
4	990.0
5	990.0
6	1762.5
7	2535.0
8	2535.0
9	2535.0
10	2961.5
11	3388.0
12	3388.0
13	3388.0
14	4917.0
15	6446.0
16	6446.0
17	6446.0
18	13234.0
19	20022.0
20	20022.0
21	20022.0
22	33521.5
23	47021.0
24	47021.0
25	47021.0
26	72802.5
27	98584.0
28	98584.0
29	98584.0
30	129764.5
31	160945.0
32	160945.0
33	160945.0
34	184780.0
35	208615.0
36	208615.0
37	208615.0
38	226243.5
39	243872.0
40	243872.0
41	243872.0
42	258001.5
43	272131.0
44	272131.0
45	272131.0
46	287836.0
47	303541.0
48	303541.0
49	303541.0
50	292920.5
51	282300.0
52	282300.0
53	282300.0
54	251278.0
55	220256.0
56	220256.0
57	220256.0
58	190110.5
59	159965.0
60	159965.0
61	159965.0
62	133293.5
63	106622.0
64	106622.0
65	106622.0
66	82527.0
67	58432.0
68	58432.0
69	58432.0
70	43114.0
71	27796.0
72	27796.0
73	27796.0
74	19808.0
75	11820.0
76	11820.0
77	11820.0
78	8082.0
79	4344.0
80	4344.0
81	4344.0
82	2895.5
83	1447.0
84	1447.0
85	1447.0
86	941.0
87	435.0
88	435.0
89	435.0
90	263.5
91	92.0
92	92.0
93	92.0
94	55.5
95	19.0
96	19.0
97	19.0
98	19.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33275287434252315
2	0.009322433074743612
3	0.008831778702388684
4	0.01052676653416025
5	0.012667803795345385
6	0.07792483531855066
7	0.07756799577501981
8	0.10183308473511801
9	0.08162704558268329
10	0.08894225622506584
11	0.0839018976726925
12	0.09500852846509039
13	0.0814040208679765
14	0.08541846573269864
15	0.08211769995503822
16	0.08425873721622336
17	0.09652509652509653
18	0.09879994861510574
19	0.09309051591861203
20	0.09215381211684354
21	0.0788169341773778
22	0.09911218321569523
23	0.09268907143213982
24	0.08863002162447634
25	0.08747029310800106
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2241904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15999879795821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36295918577768	39.531348209285575
2	13.874118403811803	14.750962353387306
3	4.96823775359082	7.92334539026576
4	2.3646671622887157	5.0282281401935744
5	1.2872631534834056	3.421545384591687
6	0.7793821771270907	2.4859173359535713
7	0.49769818438118096	1.8520344418414694
8	0.3437173129463808	1.4617609554453623
9	0.25314471792985993	1.2111455610774744
>10	1.1503667393991497	10.570592535466261
>50	0.06726188216693532	2.4696249457632238
>100	0.043658557859012445	4.418998822029637
>500	0.0051574261069599866	1.9675878776572038
>1k	0.0021982471931304036	2.3884594057742343
>5k	1.6909593793310796E-4	0.5184486412676802
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6109	0.27249159642874987	No Hit
GTCCTACAGTGGACATTTCTAAATT	5426	0.24202642039980304	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4838	0.21579871395028513	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4427	0.1974660824013874	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4156	0.18537814286427964	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3571	0.1592842512435858	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3440	0.15344100371826805	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2924	0.13042485316052782	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2534	0.1130289254133986	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2341	0.10442017142571672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.460494294135699E-5	0.0	0.0	0.0	0.0
11	4.460494294135699E-5	0.0	0.0	0.0	0.0
12	4.460494294135699E-5	0.0	0.0	8.920988588271398E-5	0.0
13	4.460494294135699E-5	0.0	0.0	8.920988588271398E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	80	1.2922828E-7	14.247288	10
GGTATCA	1795	0.0	14.116917	1
TAGGACC	865	0.0	13.287081	4
CGGTAGC	90	7.2386138E-6	11.643212	1
TACACCG	75	2.0774029E-4	11.398339	5
TAGAAAT	780	0.0	11.325272	4
AATGTCC	750	0.0	11.14554	8
AGTCGTG	60	0.0058814525	11.082213	7
GGACCTG	1645	0.0	11.029281	6
AGGACCT	1810	0.0	10.968015	5
GTCCTAA	1490	0.0	10.932828	1
GTCCTAC	2820	0.0	10.877508	1
ATTTAGA	800	0.0	10.836126	1
TTTAGAA	915	0.0	10.79624	2
AAATGTC	705	0.0	10.779052	7
TCTACAC	310	0.0	10.724244	3
ATCGCGC	205	0.0	10.657458	8
CCTATAC	135	5.7027137E-8	10.554017	3
GTATCAA	3870	0.0	10.510893	1
GAAATGT	805	0.0	10.384044	6
>>END_MODULE