FastQCFastQC Report
Thu 26 May 2016
SRR1512871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241904
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73140.32624055267308505No Hit
TATCAACGCAGAGTACTTTTTTTTT54040.24104511165509318No Hit
GTCCTACAGTGGACATTTCTAAATT51220.22846651774563048No Hit
GTCCTAAAGTGTGTATTTCTCATTT45000.20072224323610643No Hit
CTGTAGGACGTGGAATATGGCAAGA44580.19884883563256947No Hit
CTTTAGGACGTGAAATATGGCGAGG42570.1898832421013567No Hit
GGTATCAACGCAGAGTACTTTTTTT42380.18903574818547092No Hit
GTCCTACAGTGTGCATTTCTCATTT29080.1297111740734661No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25450.11351957978575353No Hit
GTACTGGTTCACTATCGGTCAGTCA24550.10950513492103142No Hit
CTGAAGGACCTGGAATATGGCGAGA22970.102457553936297No Hit
CTGTAGGACCTGGAATATGGCGAGA22430.10004888701746373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA400.00524453314.264514
GGTATCA17200.013.6578611
GTGCGCC1600.013.05334611
TCGAAAC1551.8189894E-1212.24920116
TAGGACC11450.012.1258734
CGTGCGC1459.822543E-1111.78904610
GCGCCAC1552.5465852E-1111.63713
GGCGTGC1800.011.611788
GTCGAAA1657.2759576E-1211.50708115
GGCGAGG12600.011.22575419
CCCGTAC1701.2732926E-1111.16838816
ACTGTTC4600.011.1528168
GTATTAG2050.011.1345211
CCTTACG600.00582976611.0953632
AGGACCT17950.011.0195675
TGCGCCA2000.010.91734412
ACGAAAC700.001500876610.84929317
CGAAATC2200.010.78788913
GTATCAA37900.010.7403561
TAAGGCT1951.8189894E-1210.7288634
Produced by FastQC (version 0.11.5)