FastQCFastQC Report
Thu 26 May 2016
SRR1512870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752870
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49770.2839343476698215No Hit
GTCCTACAGTGGACATTTCTAAATT45280.2583192136324998No Hit
GTCCTAAAGTGTGTATTTCTCATTT41070.23430145989149226No Hit
CTGTAGGACGTGGAATATGGCAAGA40230.22950931900255012No Hit
CTTTAGGACGTGAAATATGGCGAGG32130.18329938900203666No Hit
TATCAACGCAGAGTACTTTTTTTTT28130.16047967048326459No Hit
GGTATCAACGCAGAGTACTTTTTTT28040.15996622681659223No Hit
GTCCTACAGTGTGCATTTCTCATTT21170.12077336026060119No Hit
GTACTGGTTCACTATCGGTCAGTCA18740.10691038126044716No Hit
CTGAAGGACCTGGAATATGGCGAGA18300.10440021222338222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT602.5714311E-514.2483522
GTATACG551.9250643E-413.847821
ACCGTAT500.001500987913.2984628
TAGGACC6700.012.7597174
CCAACGA905.413858E-712.66520119
TCCCGTT802.870243E-511.87362611
TCGAACT1701.8189894E-1211.73393619
GTACCGT1001.9325362E-611.3986826
GCCTACG855.2085845E-511.2004421
GCGTGCG951.362534E-510.9984129
TAGAAAT5450.010.9803814
GGCGAGG9300.010.82670419
GTCCTAG2300.010.7621641
AAATGTC5400.010.730247
TGTAGGA22400.010.6862632
CCCCGTA2050.010.65730315
AATGTCC5550.010.6113858
GTCCTAC19950.010.5463811
TTAGGAC14150.010.5394163
GATATAC3800.010.5225211
Produced by FastQC (version 0.11.5)