##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512870_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752870
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.300120374015187	31.0	28.0	34.0	16.0	34.0
2	28.35633675058618	31.0	28.0	34.0	16.0	34.0
3	28.464871895805164	31.0	28.0	34.0	16.0	34.0
4	31.822703908447288	35.0	32.0	37.0	17.0	37.0
5	31.9781358571942	35.0	33.0	37.0	19.0	37.0
6	31.863396601002925	35.0	32.0	37.0	17.0	37.0
7	31.77482699800898	35.0	32.0	37.0	17.0	37.0
8	31.703195331085592	35.0	32.0	37.0	17.0	37.0
9	32.91509923725091	37.0	33.0	39.0	16.0	39.0
10	32.76004267287363	37.0	32.0	39.0	13.0	39.0
11	32.841306542983794	37.0	33.0	39.0	15.0	39.0
12	32.72673044777993	37.0	33.0	39.0	11.0	39.0
13	32.71447682942831	37.0	33.0	39.0	10.0	39.0
14	33.517526684808345	38.0	33.0	41.0	10.0	41.0
15	33.68748281389949	39.0	33.0	41.0	10.0	41.0
16	33.63466258193705	39.0	33.0	41.0	10.0	41.0
17	33.40399002778301	38.0	33.0	41.0	10.0	41.0
18	33.388620947360614	38.0	33.0	41.0	8.0	41.0
19	33.49987734401296	39.0	33.0	41.0	2.0	41.0
20	33.46521761454073	39.0	33.0	41.0	2.0	41.0
21	33.35396064739541	39.0	33.0	41.0	2.0	41.0
22	33.3505422535613	39.0	33.0	41.0	2.0	41.0
23	33.00860759782528	39.0	32.0	41.0	2.0	41.0
24	33.05850633532435	39.0	33.0	41.0	2.0	41.0
25	32.756944896084704	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.530422339590789
1101	2	0.24199142024054154
1101	3	0.6310087845265429
1101	4	0.6181637211082389
1101	5	0.5935376532139252
1101	6	0.5603520164552087
1101	7	0.32923743738018274
1101	8	0.6475018890819548
1101	9	0.6912102959371467
1101	10	0.661924418519618
1101	11	0.705546555524343
1101	12	0.6713053329640744
1101	13	1.0896008623786102
1101	14	1.1956912452469552
1101	15	0.9844823307802812
1101	16	0.6979764749998765
1101	17	0.15943123997309527
1101	18	0.7041318063528976
1101	19	0.027439679477957668
1101	20	-0.5513173105708304
1101	21	0.6291563463705785
1101	22	0.6713951186766707
1101	23	0.22128140105684935
1101	24	0.6453504960812921
1101	25	0.3133433485181456
1102	1	0.8940286583497787
1102	2	0.9381640629780712
1102	3	0.7864953674940409
1102	4	0.9744110220120277
1102	5	0.8547814278164729
1102	6	0.819487777499365
1102	7	0.7271681043948242
1102	8	0.8608427255143987
1102	9	1.203109232454402
1102	10	1.182712508009999
1102	11	0.8064783845096102
1102	12	1.17196399011128
1102	13	0.9143875755241027
1102	14	1.3062151572975793
1102	15	1.2531239591547276
1102	16	1.1917631166872766
1102	17	1.2795254869757358
1102	18	1.448107190595458
1102	19	1.318095835592132
1102	20	1.536179621391753
1102	21	1.6350178512778868
1102	22	1.3292099148502459
1102	23	1.1689888392223082
1102	24	0.6508193698707743
1102	25	1.105055841566518
1103	1	0.7697431911041832
1103	2	0.9160120262300921
1103	3	1.1096842627726495
1103	4	1.1051626557006458
1103	5	0.8876187466096503
1103	6	0.4575738946705634
1103	7	0.387387494856295
1103	8	0.6406663540831268
1103	9	0.6381284624629586
1103	10	0.9983595075302603
1103	11	0.7589286131702337
1103	12	0.8591056461030675
1103	13	0.9462275151159147
1103	14	1.1158624144350497
1103	15	1.0349532492164855
1103	16	1.020980026315371
1103	17	0.6883641151539877
1103	18	1.0370204973353907
1103	19	0.9472364743002331
1103	20	1.1520927830952346
1103	21	0.9964009057456664
1103	22	1.0100268910125436
1103	23	0.3359280831462428
1103	24	0.6694689790330202
1103	25	1.019353875257572
1104	1	1.0823093180265175
1104	2	1.047242592980325
1104	3	0.993241463323411
1104	4	1.2151622296981515
1104	5	0.9020177554936701
1104	6	0.9217254110526021
1104	7	1.1263330415233703
1104	8	0.8854153750870459
1104	9	1.346723947185282
1104	10	1.1594868546233954
1104	11	0.8049509581221415
1104	12	1.2958427808900055
1104	13	1.2890969931831364
1104	14	1.5168370134757723
1104	15	1.28283837104199
1104	16	1.3483463767519979
1104	17	1.6788905421315548
1104	18	1.3168524430880808
1104	19	0.9135481471920954
1104	20	0.9812473475126637
1104	21	1.0829709653436694
1104	22	0.9130952549462279
1104	23	1.357098289578083
1104	24	1.1887261602268282
1104	25	0.7408706882805944
1105	1	0.5172413122228079
1105	2	0.6641759965371747
1105	3	0.6614886481476994
1105	4	0.7190624223238018
1105	5	0.44875820575552083
1105	6	0.6757878366296133
1105	7	0.5807804249882729
1105	8	0.49058658403184197
1105	9	0.39609557874263857
1105	10	0.460221511934094
1105	11	0.5665641233689342
1105	12	0.6544130574660088
1105	13	0.6951957785021179
1105	14	0.6871214627321862
1105	15	0.741439015866014
1105	16	0.5822579293519041
1105	17	0.9930461556850858
1105	18	0.8921686322984073
1105	19	0.6143316577713449
1105	20	0.7522536417110572
1105	21	0.6548466423330908
1105	22	0.5903573104316067
1105	23	0.87351230317973
1105	24	0.8331689908996367
1105	25	1.146359663530717
1106	1	1.0378601393728921
1106	2	0.9594281972203973
1106	3	0.8357575501133923
1106	4	0.9911808720854154
1106	5	0.8852997283562267
1106	6	1.136533564244825
1106	7	1.0739257211738895
1106	8	1.107975260678753
1106	9	1.2647255399144726
1106	10	1.1159707710859195
1106	11	0.8766394086838645
1106	12	1.1347848594391223
1106	13	1.1561386325683287
1106	14	1.3479417935898255
1106	15	1.1652643833169662
1106	16	1.286874560878097
1106	17	1.4945041169078905
1106	18	1.4406591158878186
1106	19	1.3935993767284884
1106	20	1.5726112325909725
1106	21	1.5269109510276166
1106	22	1.390860345154593
1106	23	1.8027908778330684
1106	24	1.413688775124875
1106	25	1.5777426692318954
1107	1	0.9029405315254557
1107	2	0.8858944738986665
1107	3	0.8279195193476419
1107	4	0.9098059497382422
1107	5	0.8255529307965652
1107	6	1.0527433313070667
1107	7	0.6968646768521332
1107	8	0.9886873587271303
1107	9	0.9543349154285536
1107	10	1.101664494446645
1107	11	0.8840241346013684
1107	12	0.8392424058559556
1107	13	0.7440525992075351
1107	14	1.0675281456044345
1107	15	0.8274143302242436
1107	16	1.0029620574874443
1107	17	1.2471093402957862
1107	18	1.26829884284232
1107	19	0.9937137021609175
1107	20	1.167607356347098
1107	21	0.9147554109234193
1107	22	0.9462091108371737
1107	23	1.2754899840670078
1107	24	1.0413206545438385
1107	25	1.3133585874459541
1108	1	0.8416007004361141
1108	2	0.7403060656023328
1108	3	0.824584464832192
1108	4	0.8516852927585674
1108	5	0.7351819467805534
1108	6	0.8334288032385295
1108	7	0.8142660863429576
1108	8	0.8342613964442656
1108	9	0.9533870911976479
1108	10	0.9531155589848694
1108	11	0.7014510917885488
1108	12	0.970512598339532
1108	13	0.9559470242916106
1108	14	1.1038103358779452
1108	15	1.0085683957479787
1108	16	0.8895510666712312
1108	17	1.0441249091276887
1108	18	1.1451311210417074
1108	19	0.9644358777726794
1108	20	1.0430713769486815
1108	21	1.139969625304758
1108	22	1.0492974704097264
1108	23	1.4089378358884943
1108	24	1.187760965803072
1108	25	1.3151839092987885
1109	1	0.6214302833220451
1109	2	0.6607151673348248
1109	3	0.7653840796701417
1109	4	0.7998575654117062
1109	5	0.7302337634686715
1109	6	0.7967747845933992
1109	7	0.690741217347675
1109	8	0.5787303302903517
1109	9	0.7896314953205703
1109	10	0.8237626924785175
1109	11	0.7219411953032235
1109	12	0.5847846992793393
1109	13	0.8345146222018087
1109	14	0.9885444674697581
1109	15	0.6624612174754461
1109	16	0.43863014620420415
1109	17	0.9243237006057896
1109	18	0.9478325494696804
1109	19	0.8966861485897439
1109	20	0.8033769663802772
1109	21	0.7658758802234402
1109	22	0.7888926466728847
1109	23	0.9934683709726855
1109	24	0.6860897698896267
1109	25	0.31275349972937505
1110	1	0.42060324379588465
1110	2	0.48739720066878434
1110	3	0.4805089381822931
1110	4	0.4879726572672354
1110	5	0.3510792411015764
1110	6	0.3442359818250331
1110	7	0.36004188799001113
1110	8	0.39125529264115144
1110	9	0.6757333343725662
1110	10	0.5285384888411286
1110	11	0.5631143541737487
1110	12	0.5335695906382156
1110	13	0.5537397576880352
1110	14	0.6026122599468309
1110	15	0.4919844901399628
1110	16	0.7252020764831641
1110	17	0.6402447500910853
1110	18	-0.1065062415085265
1110	19	0.4542598945061016
1110	20	0.6108300288745241
1110	21	0.7795987513122427
1110	22	0.7801660337469372
1110	23	0.7859694736368112
1110	24	0.6270596550255192
1110	25	0.9041307964727991
1111	1	0.2759792851010232
1111	2	0.38033243914438586
1111	3	0.37006301637623196
1111	4	0.28221963052534704
1111	5	0.2790656758374048
1111	6	0.07516815444134295
1111	7	-0.007041916769477297
1111	8	0.2771307411922379
1111	9	0.29863027972003664
1111	10	0.2969767180115781
1111	11	-0.023806275828334833
1111	12	-0.41405454554630694
1111	13	-0.02226508117839643
1111	14	-0.08422359305866678
1111	15	0.13693490935447272
1111	16	0.1430522929248923
1111	17	-0.3274877953125568
1111	18	0.047284001142152476
1111	19	0.009694827423160746
1111	20	0.19245572786552145
1111	21	0.08487175296231442
1111	22	-0.0754296554390308
1111	23	0.17592110268602568
1111	24	0.20120162006395503
1111	25	0.12794372071943627
1112	1	0.32890978616194033
1112	2	0.14447214825167265
1112	3	0.2292746188939816
1112	4	0.38161212231657515
1112	5	0.36239612474151883
1112	6	0.0664943596783587
1112	7	-0.053115813475301366
1112	8	0.12541537508704437
1112	9	-0.10856332870091734
1112	10	0.006513470776475572
1112	11	0.05020517496053856
1112	12	0.10391015378158386
1112	13	-0.009291076602821136
1112	14	-0.04774299183298325
1112	15	-0.2625381884039655
1112	16	0.031372606814009885
1112	17	0.34516307416505043
1112	18	0.08730996100512556
1112	19	0.30414996664625704
1112	20	-0.088576739743921
1112	21	0.1455350926571981
1112	22	-0.04452473491888753
1112	23	0.11235950431710506
1112	24	0.1717094376573911
1112	25	0.09719799698453357
1113	1	0.05194063915649494
1113	2	0.18747880281912543
1113	3	0.2216460647500007
1113	4	0.33925882528087925
1113	5	0.2390204441607544
1113	6	-0.11099505350978234
1113	7	-0.06182724723210953
1113	8	-0.018444274035761765
1113	9	-0.008575427914465195
1113	10	0.10601135341410384
1113	11	0.07846893781594844
1113	12	-0.14747520617002152
1113	13	0.030672625756523075
1113	14	0.28711484240779583
1113	15	-0.17611955561510229
1113	16	-0.11407808889077131
1113	17	0.08302151336649644
1113	18	-0.09687031727678885
1113	19	-0.5419903842309353
1113	20	-0.1595386272212309
1113	21	-0.08314004345895398
1113	22	-0.5064303610531837
1113	23	-3.9534118205467763
1113	24	-1.6438320021490185
1113	25	-0.779486818502459
1114	1	-0.7752612639062448
1114	2	-0.979185881490519
1114	3	-0.8824891319989341
1114	4	-0.6595041839370772
1114	5	-0.7379584437369537
1114	6	-1.4617691639270696
1114	7	-1.298484993292174
1114	8	-1.120422666344215
1114	9	-1.3732597375586337
1114	10	-0.9676906983254128
1114	11	-1.2817332626003441
1114	12	-2.158612087922421
1114	13	-1.463794615793681
1114	14	-3.5102704286795294
1114	15	-2.151112121776265
1114	16	-2.0870902803864553
1114	17	-1.8389073200163928
1114	18	-1.8454499584701693
1114	19	-1.738467735801951
1114	20	-1.3437868183204458
1114	21	-1.176705312563918
1114	22	-1.5142844595763307
1114	23	-5.850464055654317
1114	24	-3.0580970432829275
1114	25	-2.7820500061325504
1115	1	-1.7918155138158625
1115	2	-1.7030374157849373
1115	3	-1.542501354156979
1115	4	-1.665870544907179
1115	5	-1.540176829599183
1115	6	-1.6621903078326419
1115	7	-2.203500855713745
1115	8	-2.516894356075394
1115	9	-3.9244280090074852
1115	10	-4.790575797917459
1115	11	-3.4230784558480636
1115	12	-3.661891462638529
1115	13	-2.9611183625054984
1115	14	-3.7653324629058496
1115	15	-3.3740874970514234
1115	16	-3.369440956491424
1115	17	-3.4279440299882893
1115	18	-6.158102173896758
1115	19	-4.091506981479451
1115	20	-4.182378460617674
1115	21	-3.9919700320996228
1115	22	-3.5599555409093
1115	23	-4.189852562689904
1115	24	-3.87003924687955
1115	25	-4.216394555470778
1116	1	-0.4536805234423973
1116	2	-0.3054794197832287
1116	3	-0.7241276265880359
1116	4	-0.41981582012543583
1116	5	-0.47570662307203904
1116	6	-2.780358363118687
1116	7	-1.1698432535857748
1116	8	-1.561769767106803
1116	9	-2.5086629944803285
1116	10	-1.6724108192549423
1116	11	-1.174642424455076
1116	12	-1.0644197439509178
1116	13	-2.3970770153223775
1116	14	-6.156682491903027
1116	15	-3.6934495899289743
1116	16	-2.205958631803476
1116	17	-3.3339751570675666
1116	18	-1.8359115652021742
1116	19	-1.5634008168708604
1116	20	-2.41714439439599
1116	21	-2.9782293919026124
1116	22	-2.853698589663015
1116	23	-1.85282471726914
1116	24	-2.5288823529243913
1116	25	-2.599266069299212
1201	1	0.7828104329528607
1201	2	0.8370292049064751
1201	3	1.1868103072096474
1201	4	1.018606139181614
1201	5	0.5764969322733755
1201	6	1.121629905113192
1201	7	1.17133824140625
1201	8	1.1393924376101658
1201	9	1.5546642473002095
1201	10	1.4590705274312796
1201	11	1.6094999559828977
1201	12	1.436856433139134
1201	13	1.6143391171793056
1201	14	1.6693634161173136
1201	15	1.665391825501402
1201	16	1.6629501643171665
1201	17	1.7893630052760727
1201	18	1.5683309211133007
1201	19	1.8586486717886928
1201	20	1.9129804062114033
1201	21	1.9324188871639407
1201	22	1.751495140825817
1201	23	2.062109335283978
1201	24	1.7582522317431497
1201	25	1.8998640002758975
1202	1	0.9755752115485983
1202	2	1.0529056528816483
1202	3	1.151819987678273
1202	4	1.3767003773251432
1202	5	1.1297271244684346
1202	6	1.3639380676299986
1202	7	1.3669492295971146
1202	8	1.4880071677436355
1202	9	1.704523734678844
1202	10	1.6436294808060197
1202	11	1.6200202242393544
1202	12	1.7578432736147604
1202	13	1.7437066146163644
1202	14	2.2385498012952922
1202	15	1.9533564020977536
1202	16	1.8011551345566161
1202	17	2.0157273600566583
1202	18	2.1213525617349944
1202	19	2.1648627096268243
1202	20	1.9328671171613223
1202	21	2.044395101097898
1202	22	1.9445999144195127
1202	23	2.1445215658349426
1202	24	2.171840765082237
1202	25	2.553617648929624
1203	1	1.0576622442953862
1203	2	1.2470557874164712
1203	3	1.0846906540656462
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2211	20	-0.2871923769802436
2211	21	-0.10921291795288113
2211	22	5.062111465434782E-4
2211	23	-0.020653000419535772
2211	24	-0.4309266618752545
2211	25	-0.002097190586511033
2212	1	0.12852185512745606
2212	2	0.30792042411374254
2212	3	-0.14924549604854676
2212	4	0.42636786944301264
2212	5	0.07828692933921744
2212	6	-0.227643571356122
2212	7	0.12862269227182566
2212	8	0.21731192681118472
2212	9	0.27341942991977675
2212	10	-0.043457793591336724
2212	11	-0.10709367561417338
2212	12	0.3869992342413582
2212	13	0.37828076247763676
2212	14	0.3266248242589711
2212	15	0.16914284607879182
2212	16	0.18081226198642497
2212	17	0.31139870634895317
2212	18	0.501203639166043
2212	19	0.2009459436577572
2212	20	0.4149864786468882
2212	21	0.49277647196754515
2212	22	0.556595954736288
2212	23	0.5190692744320486
2212	24	0.5239220813355558
2212	25	0.7040801808925821
2213	1	-0.659528893503019
2213	2	-0.5921043607662106
2213	3	-0.8089976843204241
2213	4	-0.6329340601764031
2213	5	-0.7017252830396643
2213	6	-0.5371177505952929
2213	7	-0.7862286672953509
2213	8	-0.471569512491282
2213	9	-0.8809514472107729
2213	10	-0.6689203917246331
2213	11	-0.7740987512186521
2213	12	-0.699464911358163
2213	13	-0.9081878316900784
2213	14	-0.923205706476395
2213	15	-0.8582122735876965
2213	16	-0.7318190502140922
2213	17	-0.7052264933070234
2213	18	-0.6386825145595267
2213	19	-0.739156336339363
2213	20	-0.6243462784909468
2213	21	-0.861803011235935
2213	22	-0.9610275248066529
2213	23	-0.7420218269487009
2213	24	-0.46740453465979925
2213	25	-0.4297771037437599
2214	1	-2.7707179047364434
2214	2	-1.6003796478616543
2214	3	-1.870774455240678
2214	4	-3.8110431155293973
2214	5	-2.0835582035971605
2214	6	-1.89528081535272
2214	7	-1.6486324005328186
2214	8	-1.4104100518579266
2214	9	-1.3671526109099048
2214	10	-1.4280536953558247
2214	11	-1.2887230459385002
2214	12	-1.4163842910263718
2214	13	-1.6866908017876696
2214	14	-1.7031784429878591
2214	15	-1.9432389670469057
2214	16	-1.7364197114481108
2214	17	-3.855955816105187
2214	18	-3.766899945426861
2214	19	-2.547292158590931
2214	20	-2.288986345187876
2214	21	-2.263891067128185
2214	22	-3.2551450319809803
2214	23	-2.1058856046274705
2214	24	-3.363946519942555
2214	25	-3.2131716948298994
2215	1	-2.2146002796144195
2215	2	-4.026791555156738
2215	3	-2.4845683151303355
2215	4	-4.322669985184589
2215	5	-2.6893904675114904
2215	6	-2.678690013452993
2215	7	-2.8198396192024973
2215	8	-2.1463765324274036
2215	9	-4.687989280464915
2215	10	-2.9242107335953236
2215	11	-2.64676379720078
2215	12	-2.5095520746417748
2215	13	-2.3917307816058155
2215	14	-2.288281162054229
2215	15	-2.4196141302018574
2215	16	-2.5344695299210898
2215	17	-2.8854461876780206
2215	18	-2.357612635500093
2215	19	-2.5927093782477684
2215	20	-2.521359943517755
2215	21	-3.0796738635583374
2215	22	-2.9572527928552326
2215	23	-2.9869216065725332
2215	24	-2.783623098386723
2215	25	-4.008646132973606
2216	1	-3.5541677302916383
2216	2	-3.7971403843682623
2216	3	-5.092223354156616
2216	4	-5.792798256977356
2216	5	-5.199335727086019
2216	6	-4.466290192833579
2216	7	-6.183008639551556
2216	8	-4.033432792452221
2216	9	-4.718877957202942
2216	10	-3.5991828314769663
2216	11	-3.9169454429718087
2216	12	-4.448647413222634
2216	13	-3.984204500423992
2216	14	-5.146662389820804
2216	15	-4.462745807107705
2216	16	-4.698337547628043
2216	17	-4.327513319670402
2216	18	-4.02692250629288
2216	19	-4.839938562625516
2216	20	-5.603158958391852
2216	21	-5.112423843153337
2216	22	-4.477227224702073
2216	23	-4.644510294957456
2216	24	-5.26990112545705
2216	25	-5.347617115687591
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111313.0
3	1.0
4	918.0
5	5867.0
6	6969.0
7	6189.0
8	5501.0
9	5018.0
10	4433.0
11	4147.0
12	3838.0
13	3932.0
14	3833.0
15	3895.0
16	4184.0
17	4407.0
18	4859.0
19	5958.0
20	7590.0
21	10011.0
22	12362.0
23	14816.0
24	17228.0
25	19281.0
26	21202.0
27	22958.0
28	26206.0
29	30559.0
30	36813.0
31	45656.0
32	56362.0
33	70553.0
34	90149.0
35	116503.0
36	155294.0
37	235391.0
38	578674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.871667187530406	18.22965855378462	12.41945214292501	24.479222115759963
2	15.067760820535526	19.800092314240413	41.6289298451711	23.503217020052958
3	19.600985440898082	25.365632121082797	28.58845518788533	26.444927250133794
4	12.659062731608403	16.141197896489338	37.44298047172088	33.756758900181374
5	13.269917742691012	38.9295538067327	34.391394619982876	13.40913383059342
6	32.36851120195492	35.757074017402424	18.22207504510471	13.652339735537943
7	29.01597712594433	31.584112853867303	21.16712265572902	18.232787364459345
8	26.250512540246547	36.25746823963698	20.1864904099294	17.305528810187074
9	28.128358001020885	14.48812216871625	19.488710264963053	37.89480956529981
10	16.839453978390736	28.182033088827296	32.9278598698328	22.05065306294917
11	35.07893222243539	21.980431386521225	24.289530060934943	18.65110633010844
12	24.834667962559955	24.17830791132572	30.655509192980933	20.33151493313339
13	29.885104723535918	21.787851122031768	25.317270947419267	23.009773207013048
14	21.817410464733186	22.213276736961053	26.800597710679025	29.16871508762674
15	23.757481149387747	30.981065454989775	22.699985497332996	22.561467898289482
16	22.503685702898657	27.437274532620897	27.643799511465932	22.415240253014513
17	22.153239258387455	28.301974066288743	27.165925156910546	22.378861518413252
18	21.898267490985678	26.586401020614446	29.883524774684737	21.631806713715136
19	24.259243714513964	26.075980303982888	27.120967875483164	22.543808106019984
20	24.797729615216603	26.348626263030017	27.57729501012137	21.27634911163201
21	24.02487067931897	25.953090564443375	26.458440386877236	23.56359836936042
22	24.476826759058483	27.575976832755313	26.337778747330955	21.60941766085525
23	23.030968536953193	27.304995520288895	27.427937751145056	22.23609819161286
24	24.10330845846426	26.012748251440787	27.7978803162681	22.086062973826856
25	23.266834313174638	26.757376217949595	27.696505004271106	22.27928446460466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	231.0
2	566.0
3	901.0
4	901.0
5	901.0
6	1528.0
7	2155.0
8	2155.0
9	2155.0
10	2485.0
11	2815.0
12	2815.0
13	2815.0
14	4015.5
15	5216.0
16	5216.0
17	5216.0
18	10303.5
19	15391.0
20	15391.0
21	15391.0
22	25764.5
23	36138.0
24	36138.0
25	36138.0
26	55584.0
27	75030.0
28	75030.0
29	75030.0
30	99103.5
31	123177.0
32	123177.0
33	123177.0
34	139784.0
35	156391.0
36	156391.0
37	156391.0
38	168958.5
39	181526.0
40	181526.0
41	181526.0
42	193388.0
43	205250.0
44	205250.0
45	205250.0
46	220245.0
47	235240.0
48	235240.0
49	235240.0
50	228618.5
51	221997.0
52	221997.0
53	221997.0
54	199356.0
55	176715.0
56	176715.0
57	176715.0
58	154275.5
59	131836.0
60	131836.0
61	131836.0
62	111025.5
63	90215.0
64	90215.0
65	90215.0
66	70335.5
67	50456.0
68	50456.0
69	50456.0
70	37705.5
71	24955.0
72	24955.0
73	24955.0
74	17833.5
75	10712.0
76	10712.0
77	10712.0
78	7537.0
79	4362.0
80	4362.0
81	4362.0
82	2949.5
83	1537.0
84	1537.0
85	1537.0
86	1011.5
87	486.0
88	486.0
89	486.0
90	297.0
91	108.0
92	108.0
93	108.0
94	65.0
95	22.0
96	22.0
97	22.0
98	15.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.329288538225881
2	0.00918493670380576
3	0.008671493037133387
4	0.010325922629744362
5	0.011352809963089105
6	0.07929852185273295
7	0.07792933874160662
8	0.10200414177891116
9	0.08306377540833033
10	0.0903660853343374
11	0.0852316486676137
12	0.0968126558158905
13	0.08266443033425183
14	0.0867719796676308
15	0.0837483669638935
16	0.08603033881577071
17	0.0977254445566414
18	0.10086315585297256
19	0.09430248677882558
20	0.09270510648251154
21	0.08043950777867155
22	0.10137659951964494
23	0.09361789522326242
24	0.0896244444824773
25	0.08933919800099266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1752870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.494048930518034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58942040189854	42.50257043342974
2	13.039509969878903	14.472304085968753
3	4.467344508936583	7.437331043052235
4	2.038025321604386	4.5239310767499425
5	1.0926695130887265	3.031832771211555
6	0.6723136567577045	2.2385644178860535
7	0.4329000806288034	1.6816364779508013
8	0.3030638428532394	1.3454591779494798
9	0.22244254781613215	1.1109813869463783
>10	1.027862330834304	10.036015887652704
>50	0.0675429372424382	2.617238924674301
>100	0.03953772660573376	4.249932695199157
>500	0.004980616465038721	1.9336103726474554
>1k	0.0023865453894977204	2.8185912486813667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4977	0.2839343476698215	No Hit
GTCCTACAGTGGACATTTCTAAATT	4528	0.2583192136324998	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4107	0.23430145989149226	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4023	0.22950931900255012	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3213	0.18329938900203666	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2813	0.16047967048326459	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2804	0.15996622681659223	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2117	0.12077336026060119	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1874	0.10691038126044716	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1830	0.10440021222338222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7114788889079053E-4	0.0
5	0.0	0.0	0.0	1.7114788889079053E-4	0.0
6	0.0	0.0	0.0	1.7114788889079053E-4	0.0
7	0.0	0.0	0.0	1.7114788889079053E-4	0.0
8	0.0	0.0	0.0	1.7114788889079053E-4	0.0
9	0.0	0.0	0.0	1.7114788889079053E-4	0.0
10	0.0	0.0	0.0	2.281971851877207E-4	0.0
11	0.0	0.0	0.0	2.281971851877207E-4	0.0
12	0.0	0.0	0.0	2.281971851877207E-4	0.0
13	0.0	0.0	0.0	2.281971851877207E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGT	60	2.5714311E-5	14.248352	2
GTATACG	55	1.9250643E-4	13.84782	1
ACCGTAT	50	0.0015009879	13.298462	8
TAGGACC	670	0.0	12.759717	4
CCAACGA	90	5.413858E-7	12.665201	19
TCCCGTT	80	2.870243E-5	11.873626	11
TCGAACT	170	1.8189894E-12	11.733936	19
GTACCGT	100	1.9325362E-6	11.398682	6
GCCTACG	85	5.2085845E-5	11.200442	1
GCGTGCG	95	1.362534E-5	10.998412	9
TAGAAAT	545	0.0	10.980381	4
GGCGAGG	930	0.0	10.826704	19
GTCCTAG	230	0.0	10.762164	1
AAATGTC	540	0.0	10.73024	7
TGTAGGA	2240	0.0	10.686263	2
CCCCGTA	205	0.0	10.657303	15
AATGTCC	555	0.0	10.611385	8
GTCCTAC	1995	0.0	10.546381	1
TTAGGAC	1415	0.0	10.539416	3
GATATAC	380	0.0	10.522521	1
>>END_MODULE