FastQCFastQC Report
Thu 26 May 2016
SRR1512870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752870
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66970.38205913730054136No Hit
TATCAACGCAGAGTACTTTTTTTTT46430.2648798827066468No Hit
GTCCTACAGTGGACATTTCTAAATT44090.25153034737316515No Hit
CTGTAGGACGTGGAATATGGCAAGA39590.2258581640395466No Hit
GTCCTAAAGTGTGTATTTCTCATTT38550.21992503722466583No Hit
GGTATCAACGCAGAGTACTTTTTTT37930.21638798085425615No Hit
CTTTAGGACGTGAAATATGGCGAGG35200.20081352296519422No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22460.1281327194829052No Hit
GTCCTACAGTGTGCATTTCTCATTT20170.11506843063090817No Hit
CTGAAGGACCTGGAATATGGCGAGA18880.10770907140860418No Hit
GTACTGGTTCACTATCGGTCAGTCA17950.10240348685298967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT400.005301177514.24118412
TAGGACC6150.012.9875924
CTAACGT604.0607434E-412.6783644
GGTATCA18300.011.7454231
GGACTTA855.2737032E-511.1867916
GCACCGT855.2737032E-511.1867916
CTGTGCG855.3323278E-511.1746589
AGGACCT14100.010.9924115
GTCCTAC19000.010.9623051
AGGACGT22600.010.7289255
GGACCTG14100.010.7226586
TTAGGAC14450.010.6606593
ATAGGAC1451.421904E-810.4927393
ATAATAC1451.421904E-810.4927393
GACGTGG10600.010.4916347
GGACGTG22350.010.4234876
CGCGCCT2100.010.39832512
GGCGAGG9850.010.21527719
GTCCTAG2053.6379788E-1210.2065771
TATCGCG2053.6379788E-1210.2007477
Produced by FastQC (version 0.11.5)