##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512870_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1752870 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.258213101941386 31.0 30.0 34.0 26.0 34.0 2 30.246674311272372 31.0 30.0 34.0 26.0 34.0 3 30.43602663061151 31.0 30.0 34.0 26.0 34.0 4 34.047343499517936 37.0 35.0 37.0 30.0 37.0 5 33.652850468089476 37.0 35.0 37.0 28.0 37.0 6 33.53153970345776 37.0 35.0 37.0 28.0 37.0 7 33.36608647532333 37.0 35.0 37.0 27.0 37.0 8 33.3068048400623 37.0 35.0 37.0 27.0 37.0 9 34.63981926782933 39.0 35.0 39.0 27.0 39.0 10 34.54404662068493 39.0 35.0 39.0 27.0 39.0 11 34.54937217249425 39.0 35.0 39.0 27.0 39.0 12 34.428551461317724 39.0 35.0 39.0 26.0 39.0 13 34.4225635671784 39.0 35.0 39.0 27.0 39.0 14 35.48880806905247 40.0 36.0 41.0 26.0 41.0 15 35.491645130557316 40.0 36.0 41.0 27.0 41.0 16 35.48277909941981 40.0 36.0 41.0 27.0 41.0 17 35.4648975679885 40.0 36.0 41.0 26.0 41.0 18 35.3920227968988 40.0 36.0 41.0 26.0 41.0 19 35.40379035524597 40.0 36.0 41.0 25.0 41.0 20 35.37953299446051 40.0 36.0 41.0 25.0 41.0 21 35.34007827163452 40.0 36.0 41.0 25.0 41.0 22 35.29645267475626 40.0 36.0 41.0 25.0 41.0 23 35.1451516655542 40.0 35.0 41.0 25.0 41.0 24 35.10505114469413 40.0 35.0 41.0 25.0 41.0 25 35.04206301665269 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5227699196128768 1101 2 0.47729152232023253 1101 3 0.47219752747858124 1101 4 0.4610640516591289 1101 5 0.5782633200242344 1101 6 0.44807110918448245 1101 7 0.6473302837135719 1101 8 0.4440001355579213 1101 9 0.825224412458617 1101 10 0.47855285038048123 1101 11 0.8065356051809829 1101 12 0.75646116818141 1101 13 0.9800478272352606 1101 14 0.9173752332917147 1101 15 0.5885764583731259 1101 16 0.8811937695458028 1101 17 0.8031342988080112 1101 18 0.727872635418997 1101 19 0.5349938728039447 1101 20 0.5981404982090837 1101 21 0.7390383247951888 1101 22 0.6523543559681784 1101 23 0.7950421475996947 1101 24 0.8230146948574983 1101 25 1.0399058337204679 1102 1 0.9745310507633214 1102 2 1.0470940339166361 1102 3 0.7630940779171169 1102 4 0.7982122370897571 1102 5 1.097247869543132 1102 6 0.9091625693217438 1102 7 0.9267077129001677 1102 8 1.002676157020204 1102 9 1.1763329448648605 1102 10 1.0466426980504835 1102 11 1.0031680651722183 1102 12 1.1450552924625583 1102 13 1.0894152056866062 1102 14 1.1372152603836554 1102 15 1.29738397824935 1102 16 1.1354245543109371 1102 17 1.3009746334804078 1102 18 0.6421314752503591 1102 19 1.052482074452449 1102 20 1.2300206692113917 1102 21 1.0206282361560923 1102 22 0.8871454342707068 1102 23 1.141089971700957 1102 24 1.0243630002134338 1102 25 1.1785419007916502 1103 1 0.7921897972223526 1103 2 0.9704639458500957 1103 3 0.6864935296184562 1103 4 0.5455187934475774 1103 5 0.7844295880527241 1103 6 0.765916628060495 1103 7 0.923413585051037 1103 8 0.9032583566857042 1103 9 1.089562348927302 1103 10 0.9339586259947481 1103 11 1.1543046743010024 1103 12 1.1666027148198808 1103 13 1.1551179884595228 1103 14 1.2950836403061459 1103 15 1.2928617313810733 1103 16 1.261968862948514 1103 17 1.2394965446349318 1103 18 1.2477045771744741 1103 19 1.2388854898074513 1103 20 1.1518715017510814 1103 21 1.2878873209659076 1103 22 1.1263384433106367 1103 23 1.3517722870913573 1103 24 1.305232818329607 1103 25 1.2041262557191814 1104 1 0.8986636400463652 1104 2 0.9754719664058342 1104 3 0.8969124794052163 1104 4 0.7636446515249702 1104 5 0.7535363106461261 1104 6 0.7534969114692416 1104 7 0.7987650059686544 1104 8 0.8755837647948539 1104 9 0.9055386133637384 1104 10 0.9743970996502256 1104 11 1.0742446054579986 1104 12 1.1203301245428534 1104 13 1.041114240135812 1104 14 1.3316218244543165 1104 15 1.3753582355448088 1104 16 1.3468129896321912 1104 17 1.2318362225820252 1104 18 0.8271357318081485 1104 19 1.2377023986685813 1104 20 1.1904648365309995 1104 21 1.1422302171002698 1104 22 1.16029383163184 1104 23 1.1314087948574283 1104 24 1.3582209268810175 1104 25 1.1160235836372223 1105 1 0.40198898765522273 1105 2 0.46159035171483964 1105 3 0.4593796232592666 1105 4 0.3016861391485648 1105 5 0.3333925773859505 1105 6 0.3349473889744843 1105 7 0.27238448546373206 1105 8 0.34534266242255995 1105 9 0.5725996760372496 1105 10 0.29136801117201827 1105 11 0.5693133105627481 1105 12 0.6214502287631589 1105 13 0.7065334124274543 1105 14 0.8022827226209301 1105 15 0.7509264176408408 1105 16 0.7874161685667005 1105 17 0.43432006163715897 1105 18 0.42583571650176566 1105 19 0.6460489722646301 1105 20 0.6981776868967131 1105 21 0.6371417017261649 1105 22 0.543061146790194 1105 23 0.6751772156938642 1105 24 0.7839783848288207 1105 25 0.6141772821817426 1106 1 0.28992697252320454 1106 2 0.5983250282563191 1106 3 -1.3288328645639886 1106 4 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0.33042801750373485 2209 19 0.40140242535122184 2209 20 0.5226783379482001 2209 21 0.5103902726807235 2209 22 0.31340135261295643 2209 23 0.48188656519539563 2209 24 0.5553079878131015 2209 25 0.40470712246046503 2210 1 -0.22350099819364644 2210 2 -1.1963046652764042 2210 3 -0.34545944086491076 2210 4 -0.022748937108630685 2210 5 -0.11964216018694174 2210 6 0.03802375871067909 2210 7 -0.08021522451787888 2210 8 -0.14476865323744903 2210 9 -0.3047447470398197 2210 10 -0.050198985434292354 2210 11 -0.18605191566908985 2210 12 -0.04024110923754165 2210 13 0.07762160493761883 2210 14 -0.00390415902738539 2210 15 0.07571280091984534 2210 16 0.07608066199642138 2210 17 0.00438033892637435 2210 18 -0.2344724451511695 2210 19 -0.48323315236723374 2210 20 0.07861391592759759 2210 21 0.013639033845890935 2210 22 0.08825124419044528 2210 23 0.0235209399589138 2210 24 -0.04641294229809034 2210 25 -0.0652698883139422 2211 1 -0.18871681248453953 2211 2 -0.37275741093480974 2211 3 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0.19486822169996998 2212 14 0.38152032473182373 2212 15 0.044105168936056316 2212 16 0.12029843681413155 2212 17 0.034669618252976875 2212 18 -0.1377953348488674 2212 19 0.1600350020484811 2212 20 0.055632836418389786 2212 21 0.2675612600287707 2212 22 0.2601928849043631 2212 23 -0.19668264839924632 2212 24 -0.1139702103180511 2212 25 -0.07713154453696802 2213 1 -0.5928573702926272 2213 2 -0.27280196645300947 2213 3 -0.3621402323522105 2213 4 -0.6247479902844049 2213 5 -0.4032598683402071 2213 6 -0.42629793371155955 2213 7 -0.9140388823156513 2213 8 -0.9471442969930237 2213 9 -0.6717439519588169 2213 10 -0.741301257558419 2213 11 -0.5580408655538491 2213 12 -0.9190941336938678 2213 13 -0.6345494448353648 2213 14 -0.8248070485793093 2213 15 -1.152750858450176 2213 16 -1.5582656342189125 2213 17 -1.0791450130878175 2213 18 -0.7737218656859213 2213 19 -0.8519349563468097 2213 20 -0.7749568160001488 2213 21 -0.7056611397831176 2213 22 -0.9797364040449708 2213 23 -1.0401224402062539 2213 24 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-4.2100494631079926 2216 22 -3.4486307732332513 2216 23 -4.889982431753644 2216 24 -4.104654893856814 2216 25 -4.195366840263691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47065.0 3 1.0 4 796.0 5 6663.0 6 8151.0 7 6371.0 8 5101.0 9 4280.0 10 3704.0 11 3337.0 12 3081.0 13 2803.0 14 2841.0 15 2877.0 16 2918.0 17 3082.0 18 3592.0 19 4095.0 20 5661.0 21 7296.0 22 9255.0 23 11252.0 24 13072.0 25 14993.0 26 16695.0 27 18437.0 28 20949.0 29 25286.0 30 31390.0 31 40376.0 32 51012.0 33 65159.0 34 84135.0 35 111406.0 36 151528.0 37 233628.0 38 730582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67263288676134 19.226100480982243 13.454941601538412 26.646325030718003 2 13.670838106032978 20.310098490257413 40.34202492590545 25.677038477804164 3 17.64060093658828 25.24548781162787 29.43103431551282 27.68287693627103 4 11.851217093740123 16.751198951153082 36.805832100055795 34.591751855051 5 12.878491778696677 38.88990009591568 34.673490126847206 13.558117998540439 6 32.32783939344986 36.31115035942845 17.85708373458522 13.503926512536468 7 28.64964107880738 31.589974640878992 21.298293368835342 18.462090911478285 8 26.14822353336693 36.55228428945615 19.910855604687015 17.388636572489897 9 27.441558708334473 14.33763112486648 19.52938840349484 38.69142176330421 10 16.23813326134193 28.63907135653382 32.993257654841926 22.129537727282326 11 34.75897173750073 21.97729095803129 24.37000151758747 18.893735786880512 12 24.342134538521485 24.412077135388557 30.812680490123036 20.433107835966922 13 29.62974584785559 21.64740476354842 25.565547998623895 23.157301389972098 14 21.55701057529442 22.066920385495926 26.938504466132184 29.437564573077466 15 23.7315503944969 30.95665212836612 22.708734163636905 22.60306331350008 16 22.503266370363608 27.363511362404502 27.522693429032756 22.61052883819913 17 21.800208354014693 28.403923810436645 27.415440333275143 22.380427502273523 18 21.731406024286102 26.480340854372386 30.09604215494086 21.692210966400648 19 23.995942579976017 26.09744882299448 27.41519752538455 22.491411071644954 20 24.570214222551844 26.233049034429644 27.87894001198049 21.317796731038026 21 23.655376610929505 25.81172591236087 26.896404182854404 23.63649329385522 22 24.155283886341632 27.385876966136053 26.801348875870218 21.6574902716521 23 22.63693257343671 27.133843354042224 27.82311295190174 22.406111120619325 24 23.96195952922921 25.9283346739918 27.992035918236947 22.11766987854205 25 22.9287967732918 26.76022751259363 28.003788073274116 22.30718764084045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 102.0 1 102.0 2 287.0 3 472.0 4 472.0 5 472.0 6 1121.5 7 1771.0 8 1771.0 9 1771.0 10 2162.0 11 2553.0 12 2553.0 13 2553.0 14 3791.0 15 5029.0 16 5029.0 17 5029.0 18 10518.5 19 16008.0 20 16008.0 21 16008.0 22 26651.0 23 37294.0 24 37294.0 25 37294.0 26 58155.5 27 79017.0 28 79017.0 29 79017.0 30 102239.5 31 125462.0 32 125462.0 33 125462.0 34 141541.0 35 157620.0 36 157620.0 37 157620.0 38 170242.0 39 182864.0 40 182864.0 41 182864.0 42 194334.0 43 205804.0 44 205804.0 45 205804.0 46 220488.0 47 235172.0 48 235172.0 49 235172.0 50 229010.0 51 222848.0 52 222848.0 53 222848.0 54 199319.0 55 175790.0 56 175790.0 57 175790.0 58 152393.0 59 128996.0 60 128996.0 61 128996.0 62 108654.5 63 88313.0 64 88313.0 65 88313.0 66 68417.5 67 48522.0 68 48522.0 69 48522.0 70 36116.5 71 23711.0 72 23711.0 73 23711.0 74 16757.0 75 9803.0 76 9803.0 77 9803.0 78 6861.0 79 3919.0 80 3919.0 81 3919.0 82 2614.0 83 1309.0 84 1309.0 85 1309.0 86 848.5 87 388.0 88 388.0 89 388.0 90 240.5 91 93.0 92 93.0 93 93.0 94 51.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08294967681573648 2 0.04090434544489894 3 0.01933971144465933 4 0.005020338074129856 5 0.01648724662981282 6 0.060529303371042925 7 0.07028473303781799 8 0.050260430037595484 9 0.019510859333550123 10 0.04615288070421651 11 0.005020338074129856 12 2.2819718518772073E-4 13 0.005761978925989948 14 6.27542259266232E-4 15 0.014718718444607986 16 0.009127887407508828 17 0.004792140888942135 18 0.005590831037099158 19 0.0018255774815017659 20 0.0011409859259386035 21 0.0 22 1.7114788889079053E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1752870.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.80272078224836 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22396975447815 39.192275502957564 2 14.303304755326854 15.105068145178583 3 4.914002956714306 7.784181781395852 4 2.273437060509191 4.801746492883292 5 1.2161261282890223 3.2107384194021 6 0.7285346450091382 2.308116686436717 7 0.4691151492810826 1.7339389369548233 8 0.3330260717896514 1.4067746145534366 9 0.24223229465952828 1.1511471797415342 >10 1.170972054970458 10.857059432198055 >50 0.072136716423108 2.6432731018307476 >100 0.04531835970945742 4.59374174405661 >500 0.004890033025072407 1.834417630797939 >1k 0.0028253524144862797 2.9932508641915945 >5k 1.086674005571646E-4 0.38426946742113843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6697 0.38205913730054136 No Hit TATCAACGCAGAGTACTTTTTTTTT 4643 0.2648798827066468 No Hit GTCCTACAGTGGACATTTCTAAATT 4409 0.25153034737316515 No Hit CTGTAGGACGTGGAATATGGCAAGA 3959 0.2258581640395466 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3855 0.21992503722466583 No Hit GGTATCAACGCAGAGTACTTTTTTT 3793 0.21638798085425615 No Hit CTTTAGGACGTGAAATATGGCGAGG 3520 0.20081352296519422 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2246 0.1281327194829052 No Hit GTCCTACAGTGTGCATTTCTCATTT 2017 0.11506843063090817 No Hit CTGAAGGACCTGGAATATGGCGAGA 1888 0.10770907140860418 No Hit GTACTGGTTCACTATCGGTCAGTCA 1795 0.10240348685298967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.7049296296930176E-5 0.0 3 0.0 0.0 0.0 5.7049296296930176E-5 0.0 4 0.0 0.0 0.0 1.1409859259386035E-4 0.0 5 0.0 0.0 0.0 1.1409859259386035E-4 0.0 6 0.0 0.0 0.0 1.1409859259386035E-4 0.0 7 0.0 0.0 0.0 1.1409859259386035E-4 0.0 8 0.0 0.0 0.0 1.1409859259386035E-4 0.0 9 0.0 0.0 0.0 1.1409859259386035E-4 0.0 10 0.0 0.0 0.0 1.1409859259386035E-4 0.0 11 0.0 0.0 0.0 1.1409859259386035E-4 0.0 12 0.0 0.0 0.0 1.1409859259386035E-4 0.0 13 0.0 0.0 0.0 1.1409859259386035E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATT 40 0.0053011775 14.241184 12 TAGGACC 615 0.0 12.987592 4 CTAACGT 60 4.0607434E-4 12.678364 4 GGTATCA 1830 0.0 11.745423 1 GGACTTA 85 5.2737032E-5 11.186791 6 GCACCGT 85 5.2737032E-5 11.186791 6 CTGTGCG 85 5.3323278E-5 11.174658 9 AGGACCT 1410 0.0 10.992411 5 GTCCTAC 1900 0.0 10.962305 1 AGGACGT 2260 0.0 10.728925 5 GGACCTG 1410 0.0 10.722658 6 TTAGGAC 1445 0.0 10.660659 3 ATAGGAC 145 1.421904E-8 10.492739 3 ATAATAC 145 1.421904E-8 10.492739 3 GACGTGG 1060 0.0 10.491634 7 GGACGTG 2235 0.0 10.423487 6 CGCGCCT 210 0.0 10.398325 12 GGCGAGG 985 0.0 10.215277 19 GTCCTAG 205 3.6379788E-12 10.206577 1 TATCGCG 205 3.6379788E-12 10.200747 7 >>END_MODULE