FastQCFastQC Report
Thu 26 May 2016
SRR1512869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032780
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56880.2798138509823985No Hit
TATCAACGCAGAGTACTTTTTTTTT32120.1580102126152363No Hit
GGTATCAACGCAGAGTACTTTTTTT31690.15589488286976455No Hit
GTACTGGTTCACTATCGGTCAGTCA25370.1248044549828314No Hit
GTCCTACAGTGGACATTTCTAAATT20640.10153582778264249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGC500.00150156813.2979492
TAGGACC4150.012.3595394
GGTATCA15300.011.954761
GTATAAT4100.011.3852691
GTACTAG700.001457538210.887371
TGACTCG700.001493664610.8554685
AGGACCT8600.010.8239135
GTATCAA34050.010.799441
CCCCGTA2300.010.73747415
TCGAACT2500.010.63835919
GTAGGAC11450.010.6184493
GTCCTAC10600.010.5150441
GTCCTAT2001.8189894E-1210.4790941
TGTAGGA12350.010.3830152
CTGTAGG11950.010.2837831
ACTATAC1303.8505277E-710.2291913
GTTCTAA2250.010.1615471
CGGTAGC750.002587781310.1615471
TAATGGA3650.010.1491194
CGCCCTA4150.010.07073616
Produced by FastQC (version 0.11.5)