FastQCFastQC Report
Thu 26 May 2016
SRR1512869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032780
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68160.3353043615147729No Hit
TATCAACGCAGAGTACTTTTTTTTT50680.24931374767559697No Hit
GGTATCAACGCAGAGTACTTTTTTT40860.2010055195348242No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23940.11776975373626265No Hit
GTACTGGTTCACTATCGGTCAGTCA23010.11319473824024243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC2200.013.80687516
GGTATCA17900.013.3930091
CCCCGTA2300.013.20657515
TACTAGC1207.2577677E-1012.6828222
TAGAACC1207.2759576E-1012.6806334
AACCCGT604.0559546E-412.680326
CCGTACA2500.012.14945317
CATCTAG1156.9412636E-811.5813951
ACTAGAC855.255809E-511.1907243
TGTGCGA855.3422315E-511.17282110
GTCTTAT1451.1896191E-911.1535111
TCTATAC1451.193257E-911.1521363
TAGGACC4100.011.1342144
GTCCTAC10550.011.0913231
CATTGCG600.005883460411.0816249
TATGCGA1158.9159767E-710.73007912
ATGCGAA1158.9159767E-710.73007913
ACCGTAT1355.6816134E-810.5565268
AGGACCT8650.010.4450315
ACGAAAT1002.4217987E-510.44119212
Produced by FastQC (version 0.11.5)