FastQCFastQC Report
Thu 26 May 2016
SRR1512868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587032
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58900.37113303323436453No Hit
TATCAACGCAGAGTACTTTTTTTTT35550.22400304467710794No Hit
GGTATCAACGCAGAGTACTTTTTTT32750.20636004819058468No Hit
GTACTGGTTCACTATCGGTCAGTCA26500.16697835960459523No Hit
GGATACCACGTGTCCCGCCCTACTC17650.1112138885668342No Hit
GTCCTACAGTGGACATTTCTAAATT16650.10491281839307587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16650.10491281839307587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.011.9999011
GGACCGC907.470784E-611.6097046
GTAGGAC8950.011.5680673
TAGGACC4100.011.3519254
GTATAAA1104.8137554E-711.25466251
GTATCAA29850.011.230011
ACACCGT951.3619594E-510.9986686
TAATACC951.3624354E-510.9983214
TGTAGGA9700.010.9673972
GCCTTAC700.001461416310.883631
ATAGACG1053.4707864E-610.8558278
ACGGTTC700.001492939410.8558278
AGGGTCG700.001493332310.8554855
GTGTAAG1951.8189894E-1210.7440971
AGACGAA909.529174E-510.55394410
ATAAGAC909.529174E-510.5539443
GATATAC1451.3889803E-810.5083331
CGAAATC1451.445369E-810.48148913
ACCCGAA1002.4016668E-510.4487337
CTGTAGG9650.010.3620051
Produced by FastQC (version 0.11.5)