##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512868_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587032
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.781122875909244	31.0	30.0	34.0	20.0	34.0
2	28.837150731680268	31.0	30.0	34.0	16.0	34.0
3	28.940490802957974	31.0	30.0	34.0	20.0	34.0
4	32.324433281748576	35.0	33.0	37.0	22.0	37.0
5	32.48319441573957	35.0	33.0	37.0	25.0	37.0
6	32.37932505456727	36.0	33.0	37.0	23.0	37.0
7	32.29342382510246	35.0	33.0	37.0	22.0	37.0
8	32.217700714289315	35.0	33.0	37.0	21.0	37.0
9	33.48443320613573	38.0	33.0	39.0	18.0	39.0
10	33.33065054768902	38.0	33.0	39.0	17.0	39.0
11	33.43302529501611	38.0	34.0	39.0	18.0	39.0
12	33.31783480106261	38.0	33.0	39.0	17.0	39.0
13	33.30516082851511	38.0	33.0	39.0	17.0	39.0
14	34.14154282963418	39.0	33.0	41.0	17.0	41.0
15	34.315679835063186	39.0	34.0	41.0	17.0	41.0
16	34.26719310007611	39.0	34.0	41.0	17.0	41.0
17	34.024737371395155	39.0	33.0	41.0	16.0	41.0
18	34.02219110893794	39.0	33.0	41.0	16.0	41.0
19	34.14509789342622	39.0	34.0	41.0	16.0	41.0
20	34.11292588933304	39.0	34.0	41.0	12.0	41.0
21	34.01287623690008	39.0	34.0	41.0	10.0	41.0
22	34.01037093139899	39.0	34.0	41.0	10.0	41.0
23	33.68628735904506	39.0	33.0	41.0	2.0	41.0
24	33.74801831343035	39.0	34.0	41.0	2.0	41.0
25	33.45387553622107	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5212877479166842
1101	2	0.3887113927984913
1101	3	0.6623839022609381
1101	4	0.7735998313750088
1101	5	0.6064275237260901
1101	6	0.6550416307508087
1101	7	0.27037159470382477
1101	8	0.7225502265293571
1101	9	0.7635682882560104
1101	10	0.7526005162423814
1101	11	0.8083272372064911
1101	12	0.6225311052321274
1101	13	0.9851947783311346
1101	14	0.9491292286239386
1101	15	0.9992490641706482
1101	16	0.8493760461589233
1101	17	0.2212185998783056
1101	18	0.6450541166373327
1101	19	0.04663759414528812
1101	20	-0.3897936134754829
1101	21	0.7835659006245663
1101	22	0.8210676695629999
1101	23	0.296033793105849
1101	24	0.8021187868320823
1101	25	0.595826340637835
1102	1	1.1599284381593975
1102	2	1.1732133613645424
1102	3	1.062781296671826
1102	4	1.378987181366071
1102	5	1.0275863657955568
1102	6	1.062029952578925
1102	7	1.0377601857855012
1102	8	1.0754056114252109
1102	9	1.3029439916283465
1102	10	1.421201098923909
1102	11	1.0786048698787312
1102	12	1.332351497683213
1102	13	1.1496906466212664
1102	14	1.2735049144667414
1102	15	1.2083794943803312
1102	16	1.4529150826659176
1102	17	1.6349010980464698
1102	18	1.4590963207301328
1102	19	1.3531259738208519
1102	20	1.5030648566173923
1102	21	1.6201510783564572
1102	22	1.3066413935009749
1102	23	1.3747610091011921
1102	24	0.8711195686490569
1102	25	1.2345022652328126
1103	1	0.7477413721702071
1103	2	0.8429943946778735
1103	3	0.9087195929780698
1103	4	1.107066798816085
1103	5	0.8449819864618178
1103	6	0.38431777282259105
1103	7	0.400034712655291
1103	8	0.5402572169145969
1103	9	0.6805718553559643
1103	10	0.8943513735459732
1103	11	0.5142083329556186
1103	12	0.6520627492832602
1103	13	0.709002723022472
1103	14	0.8759626587069889
1103	15	0.7523021956211053
1103	16	0.9052476278030426
1103	17	0.6011595572730428
1103	18	0.8587250582165922
1103	19	0.7785528662142198
1103	20	1.1332622343209025
1103	21	0.8110202984161887
1103	22	0.8384436656139584
1103	23	0.3121774367874508
1103	24	0.4951868650793898
1103	25	0.952440160357952
1104	1	0.7773729499207178
1104	2	0.8347347283272626
1104	3	0.754443249760623
1104	4	0.8100986388378644
1104	5	0.585598735899822
1104	6	0.5122223084544757
1104	7	0.6398717010771904
1104	8	0.4911708325108677
1104	9	0.8308144348554976
1104	10	0.7161819136145198
1104	11	0.6371222003428585
1104	12	0.8476830696048694
1104	13	0.9032113331528393
1104	14	1.047115917340136
1104	15	0.8321548649513488
1104	16	0.7236537778302505
1104	17	0.9849721997499827
1104	18	0.8394050454161217
1104	19	0.49691297540712753
1104	20	0.5148438572718419
1104	21	0.5601420785651072
1104	22	0.30465473564974843
1104	23	0.6995116922557472
1104	24	0.584174042525774
1104	25	0.21386525765505837
1105	1	0.579823017378736
1105	2	0.6658277231911214
1105	3	0.7180799108932696
1105	4	0.7580581147778389
1105	5	0.5453695211992908
1105	6	0.6477411584669852
1105	7	0.6422544401189825
1105	8	0.49534564792473645
1105	9	0.2654200095705974
1105	10	0.6293810696440758
1105	11	0.6656308273226941
1105	12	0.863477868922125
1105	13	0.9237124794855447
1105	14	0.9912992670681646
1105	15	0.9661837740495685
1105	16	0.8762117716378484
1105	17	1.1097154251116024
1105	18	0.889786335503743
1105	19	0.6966805660088866
1105	20	1.0068332985691129
1105	21	0.7729517147583778
1105	22	0.8087827322178782
1105	23	0.9020373826798149
1105	24	1.0674266660011682
1105	25	1.2628560766252122
1106	1	0.7340051971045796
1106	2	0.8407727223449548
1106	3	0.6743872915837414
1106	4	0.8472441022545709
1106	5	0.7602007109098992
1106	6	0.8731613332004429
1106	7	0.8460814941890504
1106	8	0.8905505260767086
1106	9	0.906023855398999
1106	10	0.8402458521791942
1106	11	0.6286903551615808
1106	12	0.749376233704659
1106	13	0.8378133214215779
1106	14	1.2231450214259283
1106	15	0.9438952099947358
1106	16	0.9934111708593747
1106	17	1.141827457604414
1106	18	1.0863456094925752
1106	19	1.085254933499158
1106	20	1.210366838508186
1106	21	1.2306632035889749
1106	22	1.179287470752314
1106	23	1.4486122578702023
1106	24	1.063480090861546
1106	25	1.275450741543132
1107	1	0.8613593001939144
1107	2	0.935046582133424
1107	3	0.8693607850622733
1107	4	0.9878109681882634
1107	5	0.9130501946271323
1107	6	0.9991917431958441
1107	7	0.6751800765016611
1107	8	0.9429645921692611
1107	9	1.020650517118824
1107	10	1.090371264996378
1107	11	0.9840750067229749
1107	12	1.0372329417227206
1107	13	0.8296412393597876
1107	14	1.1560880291388145
1107	15	0.8437380520581925
1107	16	0.9527970138425275
1107	17	1.365793846969403
1107	18	1.2620136940208155
1107	19	1.0532958938478032
1107	20	1.2258151501381391
1107	21	0.9562276265855019
1107	22	1.0305713844682955
1107	23	1.3277146286950057
1107	24	1.299101054136493
1107	25	1.4137347022889202
1108	1	0.9536311000973541
1108	2	0.9195188523759157
1108	3	0.8548036357529867
1108	4	1.0215887874970875
1108	5	0.8727007109098963
1108	6	0.8759641582778386
1108	7	0.8046363832499352
1108	8	0.961966289750599
1108	9	0.9297280607653562
1108	10	0.9912365642534908
1108	11	0.729398559495948
1108	12	0.9594771753971258
1108	13	0.9719212935577985
1108	14	1.1139419125507928
1108	15	0.9877297043187809
1108	16	0.9109331006839412
1108	17	1.0981329271606555
1108	18	1.226579097624672
1108	19	1.0450643375239252
1108	20	1.148278667713555
1108	21	1.1249295219584283
1108	22	1.0607141993384843
1108	23	1.4125083492014738
1108	24	1.1961597219245022
1108	25	1.3062099004698595
1109	1	0.80811631738176
1109	2	0.7183506797777213
1109	3	0.8361992347449352
1109	4	0.869723891068297
1109	5	0.6585157579318448
1109	6	0.8507318145566529
1109	7	0.6137804609437083
1109	8	0.65935672554874
1109	9	0.9604161792729471
1109	10	0.9726084986071726
1109	11	0.7845691292912633
1109	12	0.6779629458808714
1109	13	0.9129788262887573
1109	14	0.8853436582020322
1109	15	0.7990681393793224
1109	16	0.5037178446755775
1109	17	1.0220658671077913
1109	18	0.9309721150197205
1109	19	0.9007724326742093
1109	20	0.6414644812239132
1109	21	0.7008942401340974
1109	22	0.650445282994518
1109	23	1.0559132911155444
1109	24	0.9239416999194603
1109	25	0.4295017438529811
1110	1	0.4210662603523012
1110	2	0.4958041624658165
1110	3	0.42823341107661506
1110	4	0.3855030545227507
1110	5	0.24764252627064565
1110	6	0.3206882946352536
1110	7	0.24377509847225554
1110	8	0.38026166996629485
1110	9	0.6094895417735842
1110	10	0.5472708817819054
1110	11	0.4412426702977541
1110	12	0.5180728339863947
1110	13	0.4308443072939383
1110	14	0.4978214969679584
1110	15	0.46891651884288166
1110	16	0.6471679080281305
1110	17	0.6738800483520322
1110	18	-0.015254212891157692
1110	19	0.4596255568914245
1110	20	0.4522130141441352
1110	21	0.5543583237351513
1110	22	0.6739462229470021
1110	23	0.8417448754644212
1110	24	0.8510063122285842
1110	25	0.8065865414287998
1111	1	0.3328248034509045
1111	2	0.4190521380539636
1111	3	0.5224872046725189
1111	4	0.4444505641167922
1111	5	0.42138750040421513
1111	6	0.2624022333118532
1111	7	0.22328312693368702
1111	8	0.45152732310160815
1111	9	0.48780647676522904
1111	10	0.4321344820250701
1111	11	0.10803346622225973
1111	12	-0.1413507967168428
1111	13	0.34894575916528936
1111	14	0.2890922957146458
1111	15	0.08799150092295349
1111	16	0.4302954422493386
1111	17	0.13691710253209521
1111	18	0.3958482039343849
1111	19	0.3852774027578576
1111	20	0.4833649468351595
1111	21	0.5020094829118378
1111	22	0.10030528626326429
1111	23	0.4019182691867158
1111	24	0.4863007661901122
1111	25	0.44902463671324
1112	1	0.5273529224194249
1112	2	0.4296320979859267
1112	3	0.39228319499679287
1112	4	0.400983282629511
1112	5	0.4047350055318759
1112	6	0.20794251782118778
1112	7	0.05852784474490136
1112	8	0.21680773061376613
1112	9	0.08971583451865683
1112	10	0.2798918299451145
1112	11	0.06858887630856714
1112	12	0.195488668069018
1112	13	0.2925099873361674
1112	14	0.2811040478647797
1112	15	0.07513636456666717
1112	16	0.25516277072810567
1112	17	0.45121745637374033
1112	18	0.1345634480288993
1112	19	0.22594371031746618
1112	20	-0.05909406469975664
1112	21	0.022394715362366924
1112	22	0.024841168910413103
1112	23	0.2226234287484914
1112	24	0.19592467233178468
1112	25	0.2088312077612855
1113	1	-0.11669363791127907
1113	2	0.028457990063287752
1113	3	-0.03604655229387532
1113	4	-0.013040717711852778
1113	5	-0.15356947420234945
1113	6	-0.43945089628277856
1113	7	-0.47092339402844274
1113	8	-0.36319013280267143
1113	9	-0.4161712820359611
1113	10	-0.31519765023576696
1113	11	-0.43085119144814854
1113	12	-0.6225103293330037
1113	13	-0.4288993166870867
1113	14	-0.10106198739437389
1113	15	-0.4244192813093406
1113	16	-0.4304716518433054
1113	17	-0.3513837113182916
1113	18	-0.5027869489121031
1113	19	-0.8799923082143266
1113	20	-0.5405185274149886
1113	21	-0.2922586561879825
1113	22	-0.7289531855359002
1113	23	-4.884786556157284
1113	24	-2.238912291914513
1113	25	-1.326529096665034
1114	1	-0.9174104391794771
1114	2	-1.234271046119865
1114	3	-1.024120990230461
1114	4	-0.9174785150996279
1114	5	-0.9042223660131761
1114	6	-1.6057455151965385
1114	7	-1.457873909848594
1114	8	-1.3237627877431883
1114	9	-1.5028772141928606
1114	10	-1.15842468070187
1114	11	-1.29353650226264
1114	12	-2.328355363023647
1114	13	-1.459456962526339
1114	14	-3.173064732102908
1114	15	-1.9658408798400373
1114	16	-2.03856100881017
1114	17	-1.8041841410387676
1114	18	-1.8964130347792292
1114	19	-1.95123993054505
1114	20	-1.4803031267505915
1114	21	-1.3856701274647492
1114	22	-1.6010509141913332
1114	23	-6.104241069900887
1114	24	-3.261520763991278
1114	25	-2.9729821422515954
1115	1	-1.545856792813062
1115	2	-1.454553813347843
1115	3	-1.1504743288029573
1115	4	-1.1827332850449785
1115	5	-1.0256437904696831
1115	6	-1.18806654032403
1115	7	-1.7884191787462456
1115	8	-2.123691001940223
1115	9	-3.196229570217664
1115	10	-4.05271205500501
1115	11	-2.6532066481369654
1115	12	-2.9673032584949937
1115	13	-2.3525810134745946
1115	14	-2.7798350511317977
1115	15	-2.5723735518043256
1115	16	-2.4610075115877734
1115	17	-2.4659977814885927
1115	18	-5.022719827305963
1115	19	-3.1198887103093185
1115	20	-3.412015488577264
1115	21	-3.0134633614069806
1115	22	-2.7595298746411245
1115	23	-3.2728685433766067
1115	24	-3.039136868923112
1115	25	-3.194446425126994
1116	1	-1.4471899783340163
1116	2	-1.2259304940293134
1116	3	-1.5068078838548544
1116	4	-1.3578454445290582
1116	5	-1.5245215113123223
1116	6	-3.7150376799574545
1116	7	-2.1823815906062727
1116	8	-2.53545915959581
1116	9	-3.779420223548371
1116	10	-3.038591867119056
1116	11	-2.640749520569411
1116	12	-2.4110998262368604
1116	13	-3.6692224972918766
1116	14	-6.9914931364688115
1116	15	-4.807795213981876
1116	16	-3.799900232649396
1116	17	-4.638782922512547
1116	18	-3.175395984074676
1116	19	-2.8930208340447017
1116	20	-3.846102459737427
1116	21	-4.494003280329153
1116	22	-4.249280694452363
1116	23	-3.200876046223364
1116	24	-4.158810662062429
1116	25	-4.079127109334063
1201	1	0.5138516883326503
1201	2	0.6043472837484636
1201	3	0.866004616145144
1201	4	0.7378142776931611
1201	5	0.30978404424323003
1201	6	0.7244935700425472
1201	7	0.7770628538381743
1201	8	0.7813290348486319
1201	9	1.2998506097849614
1201	10	1.2114081328809476
1201	11	1.229128951652811
1201	12	1.2002369793186958
1201	13	1.270499724930346
1201	14	1.2484026968645239
1201	15	1.2530728415736831
1201	16	1.2584331006839378
1201	17	1.4144809663763382
1201	18	1.3041526270364372
1201	19	1.441927082621966
1201	20	1.3467100402625718
1201	21	1.3721599141152865
1201	22	1.2238269444365244
1201	23	1.5730475648877515
1201	24	1.5176303101597952
1201	25	1.6066020573326014
1202	1	1.1764447304038477
1202	2	1.1071668630364897
1202	3	1.2240021889606787
1202	4	1.4503142776931668
1202	5	1.186778380812811
1202	6	1.4743964826639058
1202	7	1.5427060577216665
1202	8	1.5198646335541355
1202	9	1.8005504479726682
1202	10	1.5304817574764158
1202	11	1.6622397930767576
1202	12	1.8205403773769504
1202	13	1.8199900161924845
1202	14	2.172973894275522
1202	15	1.963501644162676
1202	16	1.6100916767357205
1202	17	2.0907714194507747
1202	18	2.0793993907904778
1202	19	2.0645160793857187
1202	20	1.823522337997204
1202	21	1.9992756099081674
1202	22	1.9685801806824799
1202	23	2.0185734548553853
1202	24	2.1368212486711187
1202	25	2.394401410083411
1203	1	0.8785418334354311
1203	2	1.0693175578314964
1203	3	0.9144593722918586
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2211	16	-0.3324271143698212
2211	17	0.021241719064512665
2211	18	-0.2796242546839949
2211	19	-0.1528578636145923
2211	20	-0.19863942210301389
2211	21	-0.06868686007825886
2211	22	-0.09847144266024799
2211	23	-0.024156736187521233
2211	24	-0.2455686145713898
2211	25	0.02579560571970063
2212	1	0.06707812465777607
2212	2	0.1487082183526489
2212	3	-0.08735089087100434
2212	4	0.25866084904608755
2212	5	0.11916615967876254
2212	6	-0.23114979770964084
2212	7	-0.02656734208451894
2212	8	-0.09913231013415214
2212	9	0.09451866644368323
2212	10	-0.27460093516247497
2212	11	-0.4936851203620165
2212	12	0.09203834283471224
2212	13	0.04806856263222414
2212	14	-0.03374517181432424
2212	15	-0.37389499003870696
2212	16	-0.265233168842137
2212	17	-0.10044920307031191
2212	18	0.1623314064910275
2212	19	-0.06783198542145641
2212	20	0.08800736618261595
2212	21	0.21238330569590858
2212	22	0.211270351902769
2212	23	0.3646487033574317
2212	24	0.25104704301656255
2212	25	0.409763296411235
2213	1	-0.6225064234051949
2213	2	-0.39153674794640736
2213	3	-0.6785296975803448
2213	4	-0.5755049541091459
2213	5	-0.5707320046420747
2213	6	-0.34226307782207854
2213	7	-0.5587050081011249
2213	8	-0.4970076559920855
2213	9	-0.9549402175048556
2213	10	-0.5912291891711661
2213	11	-0.7569189264025198
2213	12	-0.6612211861392652
2213	13	-0.6641302777556604
2213	14	-0.8143363576613964
2213	15	-0.733951735380387
2213	16	-0.8319140655798236
2213	17	-0.6777789718455267
2213	18	-0.6278823581905328
2213	19	-0.6237833659410228
2213	20	-0.5744234542311162
2213	21	-0.8357097474479644
2213	22	-1.0238090346126256
2213	23	-0.8014159054318881
2213	24	-0.6097172590048601
2213	25	-0.3746066453265442
2214	1	-2.755715197632263
2214	2	-1.5231253039708328
2214	3	-1.7622903399952072
2214	4	-3.66097958195596
2214	5	-1.8454736311953646
2214	6	-1.6650787983785058
2214	7	-1.4651849531793708
2214	8	-1.2846139476075038
2214	9	-1.1133018024957408
2214	10	-1.1650995425576482
2214	11	-1.1854543816805219
2214	12	-1.1622191610321835
2214	13	-1.5355419417363194
2214	14	-1.4540753733109142
2214	15	-1.8102166321105315
2214	16	-1.7590559344037828
2214	17	-3.709323858185062
2214	18	-3.365545837875846
2214	19	-2.3349753296587394
2214	20	-2.2176759246497078
2214	21	-2.0900604806215526
2214	22	-2.8655316081950524
2214	23	-1.9851870842350579
2214	24	-3.0660429354542416
2214	25	-2.9757828110884468
2215	1	-1.8494335680776075
2215	2	-3.508248870097688
2215	3	-2.0366556402651135
2215	4	-3.7151504658965777
2215	5	-2.220969160884973
2215	6	-1.98316668636771
2215	7	-2.1562063769310598
2215	8	-1.549648529253929
2215	9	-4.144203877394524
2215	10	-2.2954027645549537
2215	11	-1.865342202193343
2215	12	-1.8031604565787411
2215	13	-2.039548351992728
2215	14	-1.6695780723662494
2215	15	-1.7828886968878592
2215	16	-1.8495797198288813
2215	17	-2.205022238751866
2215	18	-1.8926101934763864
2215	19	-2.000893430198552
2215	20	-1.7192995751220437
2215	21	-2.462118932038557
2215	22	-2.3478717735121926
2215	23	-2.1464556402404575
2215	24	-2.099549177019693
2215	25	-2.993590250359702
2216	1	-3.0278224633639574
2216	2	-3.1778558100639103
2216	3	-4.511557759104356
2216	4	-4.953827438873702
2216	5	-4.705942003660958
2216	6	-3.748849743330709
2216	7	-5.221532455543066
2216	8	-3.04528274159847
2216	9	-3.880096995005456
2216	10	-2.828674202448397
2216	11	-3.1342318267903018
2216	12	-3.293797451819028
2216	13	-2.9871699357482946
2216	14	-3.920943610520709
2216	15	-3.5621916294842038
2216	16	-3.8787674981184566
2216	17	-3.480800969751403
2216	18	-2.954757053602286
2216	19	-3.836675711789212
2216	20	-4.4374416563442125
2216	21	-3.9800182295972846
2216	22	-3.441425051571457
2216	23	-3.5409095209405947
2216	24	-3.9646151988222407
2216	25	-3.9594258867791723
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85463.0
3	5.0
4	755.0
5	4537.0
6	5772.0
7	5256.0
8	4598.0
9	4186.0
10	3621.0
11	3505.0
12	3259.0
13	3210.0
14	3208.0
15	3206.0
16	3283.0
17	3554.0
18	4165.0
19	4806.0
20	6256.0
21	8196.0
22	10398.0
23	12505.0
24	14397.0
25	16267.0
26	17800.0
27	19563.0
28	22117.0
29	26108.0
30	31617.0
31	39123.0
32	48916.0
33	62019.0
34	80075.0
35	104472.0
36	140771.0
37	215292.0
38	564751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83193192307206	17.832450339499538	12.22337425872773	24.11224347870067
2	16.01845660951176	19.736182907470553	39.919539063696355	24.32582141932134
3	19.794136144394578	25.14711312933478	28.241309643991897	26.81744108227874
4	12.989263628738462	15.58245302483097	36.64129353202479	34.786989814405786
5	14.029175037622036	38.32437630605809	33.396960996599525	14.24948765972035
6	33.188587759518875	34.78976472643276	17.830632544043247	14.191014970005128
7	29.6709928482738	31.41695742954058	20.640180707050387	18.271869015135227
8	27.354664291607477	34.27610315359753	20.066432693059763	18.302799861735224
9	28.36030041010305	14.40115683998035	19.08338215512528	38.15516059479132
10	17.460238374241758	27.600225533466116	31.749322327434733	23.190213764857397
11	35.558526656344895	22.314704741603574	22.986671039230092	19.14009756282144
12	24.685249274501235	24.32169570869293	29.50775831130504	21.485296705500797
13	29.795120942211597	21.200454558067143	25.21718895439032	23.787235545330944
14	22.774523894963945	21.260359466633705	26.415922534114266	29.549194104288084
15	24.84765124669619	28.853904750733285	22.903111679535034	23.395332323035483
16	23.848921430440857	27.036921417827852	25.56138907020083	23.552768081530463
17	22.95940700597813	27.299889116374825	26.252360534512054	23.48834334313499
18	22.841633594295992	26.100471044750513	28.019909020494353	23.037986340459135
19	24.44551384339038	26.02083099446113	26.79306878113328	22.740586381015213
20	25.224987717048737	25.352011408165463	26.841102444543814	22.58189843024198
21	25.158442777090407	25.411762035029568	26.10209957963003	23.327695608249996
22	25.09007167889068	26.064775918314826	26.009017259953932	22.836135142840565
23	24.239312724573274	25.84655886098264	26.587835941437028	23.326292473007058
24	24.360343269358726	25.47388111709491	26.736639091005987	23.429136522540375
25	24.04921509752392	25.851674843325377	26.819818984605938	23.279291074544766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	232.0
1	232.0
2	558.5
3	885.0
4	885.0
5	885.0
6	1558.5
7	2232.0
8	2232.0
9	2232.0
10	2484.0
11	2736.0
12	2736.0
13	2736.0
14	3633.5
15	4531.0
16	4531.0
17	4531.0
18	8794.0
19	13057.0
20	13057.0
21	13057.0
22	20663.0
23	28269.0
24	28269.0
25	28269.0
26	42791.5
27	57314.0
28	57314.0
29	57314.0
30	73354.5
31	89395.0
32	89395.0
33	89395.0
34	104852.5
35	120310.0
36	120310.0
37	120310.0
38	133025.0
39	145740.0
40	145740.0
41	145740.0
42	160963.5
43	176187.0
44	176187.0
45	176187.0
46	194472.5
47	212758.0
48	212758.0
49	212758.0
50	212665.0
51	212572.0
52	212572.0
53	212572.0
54	196836.5
55	181101.0
56	181101.0
57	181101.0
58	160592.5
59	140084.0
60	140084.0
61	140084.0
62	119180.0
63	98276.0
64	98276.0
65	98276.0
66	76855.5
67	55435.0
68	55435.0
69	55435.0
70	41494.5
71	27554.0
72	27554.0
73	27554.0
74	19505.0
75	11456.0
76	11456.0
77	11456.0
78	8076.5
79	4697.0
80	4697.0
81	4697.0
82	3141.5
83	1586.0
84	1586.0
85	1586.0
86	1035.5
87	485.0
88	485.0
89	485.0
90	293.0
91	101.0
92	101.0
93	101.0
94	66.5
95	32.0
96	32.0
97	32.0
98	19.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3334526335952898
2	0.011026872804077045
3	0.009955690874538133
4	0.011530958417977709
5	0.012350097540566289
6	0.0794564948910923
7	0.0794564948910923
8	0.10510185049828864
9	0.08399326541619828
10	0.09186960313339618
11	0.08739584331002777
12	0.09898981242974307
13	0.08292208348665937
14	0.08853003594130428
15	0.08575756506485062
16	0.08613562927527611
17	0.0999349729558068
18	0.10207733681488465
19	0.0951461596237505
20	0.09457906330811225
21	0.08103176243453188
22	0.10258142242878532
23	0.09609132014981425
24	0.09168057102818343
25	0.0895382071691056
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1587032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.55969929873028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55587786577482	39.18634521577985
2	13.531352982270725	14.22407687706253
3	4.929511745648596	7.772809651225476
4	2.241899208508595	4.713341930290927
5	1.332566281722296	3.5019641531475494
6	0.7859105200287944	2.4784332365053303
7	0.5391025388280043	1.9834547132387383
8	0.3638716202367615	1.5299986354388773
9	0.26429953661364963	1.2502353752286461
>10	1.3238756834635317	12.309127308610133
>50	0.08098068407566388	2.9384081403867275
>100	0.04469530190573023	4.412931012552891
>500	0.003875859790689546	1.5254997450046357
>1k	0.0020590505138038213	1.7984128780361792
>5k	1.2112061845904832E-4	0.374961127491437
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5890	0.37113303323436453	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3555	0.22400304467710794	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3275	0.20636004819058468	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2650	0.16697835960459523	No Hit
GGATACCACGTGTCCCGCCCTACTC	1765	0.1112138885668342	No Hit
GTCCTACAGTGGACATTTCTAAATT	1665	0.10491281839307587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1665	0.10491281839307587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.301070173758312E-5
7	0.0	0.0	0.0	0.0	6.301070173758312E-5
8	0.0	0.0	0.0	0.0	6.301070173758312E-5
9	0.0	0.0	0.0	0.0	1.8903210521274932E-4
10	0.0	0.0	0.0	0.0	1.8903210521274932E-4
11	0.0	0.0	0.0	0.0	2.5204280695033247E-4
12	0.0	0.0	0.0	0.0	2.5204280695033247E-4
13	0.0	0.0	0.0	0.0	2.5204280695033247E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1365	0.0	11.999901	1
GGACCGC	90	7.470784E-6	11.609704	6
GTAGGAC	895	0.0	11.568067	3
TAGGACC	410	0.0	11.351925	4
GTATAAA	110	4.8137554E-7	11.2546625	1
GTATCAA	2985	0.0	11.23001	1
ACACCGT	95	1.3619594E-5	10.998668	6
TAATACC	95	1.3624354E-5	10.998321	4
TGTAGGA	970	0.0	10.967397	2
GCCTTAC	70	0.0014614163	10.88363	1
ATAGACG	105	3.4707864E-6	10.855827	8
ACGGTTC	70	0.0014929394	10.855827	8
AGGGTCG	70	0.0014933323	10.855485	5
GTGTAAG	195	1.8189894E-12	10.744097	1
AGACGAA	90	9.529174E-5	10.553944	10
ATAAGAC	90	9.529174E-5	10.553944	3
GATATAC	145	1.3889803E-8	10.508333	1
CGAAATC	145	1.445369E-8	10.481489	13
ACCCGAA	100	2.4016668E-5	10.448733	7
CTGTAGG	965	0.0	10.362005	1
>>END_MODULE