FastQCFastQC Report
Thu 26 May 2016
SRR1512868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587032
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77140.4860645532037161No Hit
TATCAACGCAGAGTACTTTTTTTTT55260.34819713780188427No Hit
GGTATCAACGCAGAGTACTTTTTTT44730.2818468688722093No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27250.17170416223491397No Hit
GTACTGGTTCACTATCGGTCAGTCA23920.1507215985562988No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17110.1078113106730047No Hit
GGATACCACGTGTCCCGCCCTACTC16100.10144722979750882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.013.7168011
GTCTTAT1001.4200305E-712.3648781
TGGTCGG853.964138E-612.28836710
AGATCGT550.003042387812.104716
GACGTGA4750.012.0064957
TAAGCTG1052.6798443E-711.775335
TCGCTTC658.074323E-411.68352513
CGAATTT658.074323E-411.68352515
TAGGACC3600.011.6243644
TCTACAC2000.011.4148143
TTAGGAC6250.011.4148133
GTCCTAA5300.011.1265951
CGGACTG951.3430417E-511.0125555
TAGGACG9000.010.9903084
GGACGTG8850.010.8541836
CCCCGGT2400.010.67913519
CTACACT2050.010.6707034
TTAACCT3150.010.5676044
TTAAGCT1355.5910277E-810.5676034
ATTGCGA909.55968E-510.55061810
Produced by FastQC (version 0.11.5)