##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512868_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587032
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.08190635097465	31.0	30.0	34.0	26.0	34.0
2	30.063728393630374	31.0	30.0	34.0	26.0	34.0
3	30.239119942130973	31.0	30.0	34.0	26.0	34.0
4	33.846648334753176	37.0	35.0	37.0	30.0	37.0
5	33.40690924946693	37.0	35.0	37.0	28.0	37.0
6	33.26458130648909	37.0	35.0	37.0	27.0	37.0
7	33.09404725298545	37.0	35.0	37.0	26.0	37.0
8	33.036225482535954	37.0	35.0	37.0	26.0	37.0
9	34.3319082412957	39.0	35.0	39.0	25.0	39.0
10	34.25888261862395	39.0	35.0	39.0	25.0	39.0
11	34.24592509791863	39.0	35.0	39.0	25.0	39.0
12	34.097640123198524	39.0	35.0	39.0	25.0	39.0
13	34.08941218576563	39.0	35.0	39.0	25.0	39.0
14	35.14458057556495	39.0	35.0	41.0	25.0	41.0
15	35.134610392229014	39.0	35.0	41.0	25.0	41.0
16	35.13348628131002	39.0	35.0	41.0	25.0	41.0
17	35.11616085876025	39.0	35.0	41.0	25.0	41.0
18	35.03961734861049	39.0	35.0	41.0	24.0	41.0
19	35.05203108695981	40.0	35.0	41.0	24.0	41.0
20	35.017417418174304	40.0	35.0	41.0	24.0	41.0
21	34.97984728726327	40.0	35.0	41.0	24.0	41.0
22	34.94590531255829	40.0	35.0	41.0	23.0	41.0
23	34.78729351393041	39.0	35.0	41.0	22.0	41.0
24	34.73601792528443	39.0	35.0	41.0	21.0	41.0
25	34.66600232383468	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7326187871596304
1101	2	0.5138495772311913
1101	3	0.539437773272816
1101	4	0.5883585936181746
1101	5	0.6544565744173028
1101	6	0.6685669504035587
1101	7	0.6581190551865888
1101	8	0.4819719893330259
1101	9	0.780532783196584
1101	10	0.4573318767395591
1101	11	0.8068112364903968
1101	12	0.7885532970515285
1101	13	1.055100571337718
1101	14	0.9620148411443736
1101	15	0.8084425406769782
1101	16	0.9199322598104374
1101	17	0.9964018653319968
1101	18	0.8935695627436644
1101	19	0.7245510970532791
1101	20	0.8827721334515886
1101	21	0.9360205865926261
1101	22	0.7743632060592489
1101	23	0.8798473191790563
1101	24	0.8958693581605175
1101	25	0.8843169041938808
1102	1	0.8156391599776427
1102	2	0.8758140063119768
1102	3	0.566170304221199
1102	4	0.6320193902300701
1102	5	0.8651567739033368
1102	6	0.7158198979937751
1102	7	0.7310574949884341
1102	8	0.8206877059576811
1102	9	0.8125935896250098
1102	10	0.8879931256132849
1102	11	1.0204169553237747
1102	12	0.9010131106074653
1102	13	1.0271870760549149
1102	14	0.8764619773453077
1102	15	1.05346678401488
1102	16	1.034341006343972
1102	17	1.0327710792728837
1102	18	0.33656108195778245
1102	19	0.7537726457520648
1102	20	0.9748172334224847
1102	21	0.9845332061001812
1102	22	0.7091126915675829
1102	23	1.0994851240613812
1102	24	0.9343692862894457
1102	25	1.008791281282086
1103	1	0.7939203029358097
1103	2	0.9875086054963553
1103	3	0.7506371158343725
1103	4	0.5882014040572159
1103	5	0.7514153011267055
1103	6	0.7928218512017793
1103	7	0.8976425088342808
1103	8	0.916252274866487
1103	9	1.0395315408475057
1103	10	1.027685236114884
1103	11	1.2157957229838132
1103	12	1.3069565406719121
1103	13	1.2614515121999759
1103	14	1.3809939638887414
1103	15	1.28079027707836
1103	16	1.2193366630485372
1103	17	1.2164844863714066
1103	18	1.2482760801813626
1103	19	1.113170228125938
1103	20	0.9039479113675597
1103	21	1.1532062316006844
1103	22	1.1862956862485348
1103	23	1.247967690532498
1103	24	1.3596795641753587
1103	25	1.1658737426981531
1104	1	0.5947757330682677
1104	2	0.8016265642889842
1104	3	0.6536610132354888
1104	4	0.46492913003218916
1104	5	0.52137245381914
1104	6	0.36414576082991346
1104	7	0.42506844389659904
1104	8	0.42871427964325903
1104	9	0.5078370239252763
1104	10	0.4994421339184001
1104	11	0.7032565989856678
1104	12	0.694539126624413
1104	13	0.5098978400232355
1104	14	0.9103829786181805
1104	15	0.7958850463847753
1104	16	0.76898727013171
1104	17	0.699197751503668
1104	18	0.32049921970486395
1104	19	0.805301562819075
1104	20	0.7120462864919261
1104	21	0.6494956106030969
1104	22	0.5483534577732811
1104	23	0.640585930942116
1104	24	0.8325464514557268
1104	25	0.6481381344136992
1105	1	0.6162301046200334
1105	2	0.6308333590805262
1105	3	0.6520369338981205
1105	4	0.39977446907839465
1105	5	0.49229811262024015
1105	6	0.689182600295382
1105	7	0.6434835433469601
1105	8	0.6830938667561242
1105	9	0.9645472499388035
1105	10	0.7044194146279992
1105	11	0.977238054186671
1105	12	1.0742036768848493
1105	13	1.0380087498187152
1105	14	1.1546663813535716
1105	15	1.2025507863947027
1105	16	1.079714391106421
1105	17	0.8589820478471921
1105	18	0.784619621575807
1105	19	1.1421949104605034
1105	20	1.2269665940123602
1105	21	0.953669551201159
1105	22	1.0072435951647236
1105	23	1.168025176604651
1105	24	0.981391500192693
1105	25	0.919892049376422
1106	1	0.41564360521235244
1106	2	0.5892640897456118
1106	3	-1.2619290501939453
1106	4	-0.10878239333487016
1106	5	0.19092785115467592
1106	6	0.3378951045262184
1106	7	0.4452504455300712
1106	8	0.44052559166727434
1106	9	0.6768068856639644
1106	10	0.477285751994728
1106	11	0.5483795332863508
1106	12	0.6533229229501742
1106	13	0.6256134629681469
1106	14	0.6042811110032531
1106	15	0.6780846648005223
1106	16	0.7732539070989759
1106	17	0.7572556030172706
1106	18	0.8134593681488269
1106	19	0.7981738726368448
1106	20	0.8543768474393403
1106	21	0.7516776215176293
1106	22	0.7156928357299677
1106	23	0.7940062013127189
1106	24	0.7752745070466389
1106	25	0.8673543420655392
1107	1	0.5465517246471556
1107	2	0.6918716965555269
1107	3	0.5798208766485793
1107	4	0.4930763291265663
1107	5	0.5696268760700249
1107	6	0.47066971940435565
1107	7	0.5112718022877303
1107	8	0.3372112313771538
1107	9	0.39141336375852376
1107	10	0.47580594607106264
1107	11	0.4774899383697502
1107	12	0.680037812586896
1107	13	0.5923202791547624
1107	14	0.6958563090509955
1107	15	0.6787865193425588
1107	16	0.7553878061834638
1107	17	0.7667742398844624
1107	18	0.7907175173691812
1107	19	0.6763474047843303
1107	20	0.6672547188702964
1107	21	0.7435065192434749
1107	22	0.6832445554532214
1107	23	0.7198408497387874
1107	24	0.8693148507822741
1107	25	0.8495212273337103
1108	1	0.5106365102691086
1108	2	0.4069256609530427
1108	3	0.42129076404754073
1108	4	0.24659686363107625
1108	5	0.4698178150349648
1108	6	0.4059230328027894
1108	7	0.4410212143639214
1108	8	0.25554074904601976
1108	9	0.5418626777176314
1108	10	0.43142307139617486
1108	11	0.5352422783843878
1108	12	0.517215466809823
1108	13	0.4875368375811462
1108	14	0.6526586264831309
1108	15	0.5698974227984621
1108	16	0.5128049906903058
1108	17	0.6133988563712478
1108	18	0.681142224185983
1108	19	0.73578642423643
1108	20	0.7171106580688544
1108	21	0.6590837747683977
1108	22	0.6042967443986953
1108	23	0.7088520475459532
1108	24	0.6556011969331266
1108	25	0.7028461506310677
1109	1	0.2045424049153688
1109	2	0.16871802680220327
1109	3	0.23623544411392672
1109	4	0.15914987856745455
1109	5	-0.234613004314717
1109	6	-0.04796196320187818
1109	7	0.17846020877045987
1109	8	0.1879308775111994
1109	9	0.25514714510960346
1109	10	-0.25521378397293404
1109	11	0.04054254269073709
1109	12	0.3263116005611266
1109	13	0.07996814866357482
1109	14	0.19358800136787835
1109	15	0.3432814660709411
1109	16	0.22590198497391611
1109	17	0.3413034602190521
1109	18	0.5293517023346084
1109	19	0.3586231074990778
1109	20	0.5284727577676662
1109	21	0.43289900619011945
1109	22	0.6093714263090817
1109	23	0.5948564116740442
1109	24	0.5185731067001669
1109	25	0.5003988582344903
1110	1	0.2662105226514093
1110	2	0.141339022884317
1110	3	0.23523317266343113
1110	4	0.29923228552674885
1110	5	0.36357099047116037
1110	6	0.4643391936814183
1110	7	0.303790955309168
1110	8	0.32747034943543696
1110	9	0.3088244507899276
1110	10	0.662547365954957
1110	11	0.41203352863158216
1110	12	0.5080559115990653
1110	13	0.44928465850936306
1110	14	0.5321484270581323
1110	15	0.38117170442732373
1110	16	0.20734704335310994
1110	17	0.3634282148951087
1110	18	0.4196326180161236
1110	19	0.45212041167159356
1110	20	0.5911778023819707
1110	21	0.6481724435951222
1110	22	0.6210959047144939
1110	23	0.6856417766260989
1110	24	0.5610551892360149
1110	25	0.571334566944067
1111	1	0.19445615808119499
1111	2	0.05237727162475281
1111	3	-0.0211359659545316
1111	4	0.2038362391931372
1111	5	0.1864568877553907
1111	6	0.32883277215913864
1111	7	0.15465200890047015
1111	8	0.13665788112032828
1111	9	0.05191794247347303
1111	10	-0.061187131446232
1111	11	2.611483392698233E-4
1111	12	0.19279628478970068
1111	13	0.13451541576271353
1111	14	-0.012482148760717848
1111	15	0.1925309152389474
1111	16	0.1378194025703081
1111	17	0.14860319761381646
1111	18	0.20262999138223847
1111	19	0.07457106078803122
1111	20	0.23388762365712523
1111	21	0.3021211073023551
1111	22	0.040782551579269466
1111	23	0.07715528887383982
1111	24	0.26581372412353943
1111	25	0.21467572918926692
1112	1	-0.03231643396392059
1112	2	-0.39909423726345494
1112	3	-0.05886878847183041
1112	4	0.011087258386012877
1112	5	-0.08266059799821335
1112	6	-0.09271924250172248
1112	7	0.12128407866786262
1112	8	0.06172814684223482
1112	9	0.14490854142554355
1112	10	-0.239807653161769
1112	11	-0.10263371690850676
1112	12	0.3124246120423493
1112	13	0.3531165909166205
1112	14	-0.01896486624989535
1112	15	-0.20546944101643305
1112	16	-0.19039522938213338
1112	17	-0.02771556601501146
1112	18	0.19129214853607834
1112	19	0.025277353889663345
1112	20	-0.05032167729709158
1112	21	-0.02867469572813519
1112	22	0.09868673110265291
1112	23	0.4316365752307618
1112	24	0.28632392094088743
1112	25	0.25578304791531536
1113	1	-0.8976593295319546
1113	2	-0.7864645111851303
1113	3	-0.680349066362762
1113	4	-2.2138576923543773
1113	5	-0.9356997192729679
1113	6	-0.6676375012966318
1113	7	-0.872958197014654
1113	8	-0.6499448134341961
1113	9	-0.6600517706138902
1113	10	-0.8123205670596008
1113	11	-0.9692455159456301
1113	12	-0.5522895876735703
1113	13	-0.6573492917425767
1113	14	-0.9773077799297667
1113	15	-0.9943871110229452
1113	16	-1.2629056874367919
1113	17	-1.2252655861682769
1113	18	-1.1358992233501013
1113	19	-0.9387451575967702
1113	20	-0.7044247619282089
1113	21	-0.6694985758559753
1113	22	-0.8168250497305323
1113	23	-0.6125174896348113
1113	24	-0.7166610029448606
1113	25	-0.9440423109370926
1114	1	-1.2130510687125877
1114	2	-2.412751482253512
1114	3	-1.6583619203110231
1114	4	-1.0898101315994495
1114	5	-1.2267351179885537
1114	6	-0.8837643015904249
1114	7	-0.8697741058431632
1114	8	-0.9028050175350444
1114	9	-1.2647452877138647
1114	10	-1.5926721550519929
1114	11	-1.42074728584047
1114	12	-1.3921261825318254
1114	13	-1.5811289322610946
1114	14	-1.4399621390788084
1114	15	-1.7003793698312748
1114	16	-1.9506901786872177
1114	17	-1.896542850041051
1114	18	-1.567804822408128
1114	19	-2.1023604103809888
1114	20	-1.5278854692802142
1114	21	-1.8117245901576098
1114	22	-1.4700972876423961
1114	23	-1.2551926306163708
1114	24	-1.1159425640224043
1114	25	-1.7122066397158378
1115	1	-0.9842436554353284
1115	2	-0.9100281288403664
1115	3	-0.4375197810613187
1115	4	-0.15976080754145272
1115	5	-0.4781134299896479
1115	6	-0.3703701032927995
1115	7	-0.2921825850904298
1115	8	-0.49317326395374295
1115	9	-1.3603693857817944
1115	10	-1.0063253886500405
1115	11	-1.2863662294255107
1115	12	-2.089913688360326
1115	13	-2.0200510962916525
1115	14	-1.5637136520301667
1115	15	-1.926907665811676
1115	16	-1.7167389356701435
1115	17	-1.6148161884168672
1115	18	-2.0666302722781182
1115	19	-2.3505812943133435
1115	20	-2.3687681396465265
1115	21	-1.7453494372235312
1115	22	-1.651981861643641
1115	23	-1.6861185445537217
1115	24	-2.229884825619372
1115	25	-2.056635250127897
1116	1	-1.3080634488812173
1116	2	-1.3402863488508778
1116	3	-1.4384641379132432
1116	4	-1.0089331608787333
1116	5	-1.6347757307166688
1116	6	-2.391956869157994
1116	7	-1.9218913673354265
1116	8	-2.243387968274245
1116	9	-3.517226374569951
1116	10	-2.1816141018064386
1116	11	-2.9811343556025385
1116	12	-5.107801894301289
1116	13	-3.9672456379090484
1116	14	-3.418334020575692
1116	15	-4.425830722626376
1116	16	-3.887930303427339
1116	17	-3.7739030227137214
1116	18	-3.7652824081669607
1116	19	-3.9117585104040913
1116	20	-5.635457765133761
1116	21	-4.478288570002842
1116	22	-3.666068860176466
1116	23	-4.601141825003069
1116	24	-4.638422495877336
1116	25	-3.7375204751859243
1201	1	0.5220580788965599
1201	2	0.7764844844824559
1201	3	0.7852858620867593
1201	4	0.6446605891212656
1201	5	-0.05890767516110884
1201	6	0.519918130842008
1201	7	0.7119280771090146
1201	8	0.5703446706146451
1201	9	0.5224264032078239
1201	10	0.6532122870824466
1201	11	0.3300461999530171
1201	12	0.7980488001431567
1201	13	0.9382231120909523
1201	14	0.7168743127576391
1201	15	0.755019971818065
1201	16	0.7649863632393306
1201	17	0.5588400328418359
1201	18	0.8914363418330424
1201	19	0.9533109340403527
1201	20	0.972649873755131
1201	21	0.588569068886045
1201	22	0.6950075287124236
1201	23	0.829587341663597
1201	24	0.5777580596782244
1201	25	0.7213510525918565
1202	1	1.0514674640098285
1202	2	1.0984019602106088
1202	3	1.0682106193683119
1202	4	1.036412207244247
1202	5	1.058648959143099
1202	6	1.1390402991267976
1202	7	1.2889871385976903
1202	8	1.0800048647894016
1202	9	1.4739919371884085
1202	10	1.4963878534254889
1202	11	1.4468423164578752
1202	12	1.5480730719878153
1202	13	1.5002781282721855
1202	14	1.659285315993884
1202	15	1.527273208064024
1202	16	1.6353302143720114
1202	17	1.6632008742657831
1202	18	1.6125366654576325
1202	19	1.7820285715808097
1202	20	1.597269614855449
1202	21	1.6205391336109685
1202	22	1.533878887935721
1202	23	1.7759061118901371
1202	24	1.7935994836264442
1202	25	1.8129489490319841
1203	1	0.9454407874818109
1203	2	0.9693008891579886
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2211	13	0.05771235940277819
2211	14	-0.07549127864020733
2211	15	-0.03359561957978485
2211	16	0.05642453528233915
2211	17	-0.20250405317967335
2211	18	-0.2406604323605066
2211	19	-0.1971863777876024
2211	20	-0.12638238430938742
2211	21	-0.20751964079137508
2211	22	-0.1494413960187586
2211	23	-0.3124825508095199
2211	24	-0.016838714515330366
2211	25	-0.16062475385975716
2212	1	-0.2286667026761151
2212	2	-0.04522123434200864
2212	3	-0.044881267910593436
2212	4	-0.09230889724356928
2212	5	-0.4486415909673056
2212	6	-0.22194346566315915
2212	7	-0.49230246300218283
2212	8	-0.31449647315024976
2212	9	-0.3778479547482263
2212	10	-0.512279836295896
2212	11	-0.22068321492651677
2212	12	0.043024309806909855
2212	13	-0.05065033244174799
2212	14	0.09617402152120746
2212	15	-0.20524842902916873
2212	16	-0.2958433296386147
2212	17	-0.4635673010210226
2212	18	-0.42651838413995335
2212	19	-0.25534441411956266
2212	20	-0.23941724300420475
2212	21	-0.11272858798448482
2212	22	-0.06139404661190895
2212	23	-0.22654517091252302
2212	24	-0.35228959719231057
2212	25	-0.31132071071235856
2213	1	-0.6955789074048084
2213	2	-0.19952075322906992
2213	3	-0.3708607266857378
2213	4	-0.6177866980127646
2213	5	-0.3536901086824642
2213	6	-0.25818118422648695
2213	7	-0.7653070155581858
2213	8	-0.6774272874836669
2213	9	-0.5150537874985943
2213	10	-0.40415039086543914
2213	11	-0.4739814659078476
2213	12	-0.6808720969828173
2213	13	-0.42264849682658934
2213	14	-0.47319149438713737
2213	15	-0.8523373341180047
2213	16	-1.2978315927101747
2213	17	-0.8442267144050106
2213	18	-0.6006627717850108
2213	19	-0.5262173361718396
2213	20	-0.5783231318854831
2213	21	-0.5312466039584365
2213	22	-0.6734308946021059
2213	23	-0.8454697359619701
2213	24	-0.4928060015627125
2213	25	-0.45511248487792955
2214	1	-1.0531063947876476
2214	2	-0.4164924892017545
2214	3	-0.6270606291413117
2214	4	-0.6375652880717126
2214	5	-0.8719833330558515
2214	6	-1.4861180533685214
2214	7	-0.8767067913120385
2214	8	-0.6207408557011433
2214	9	-0.5300187722307683
2214	10	-0.972960958531587
2214	11	-0.963133624608389
2214	12	-0.6723349717866682
2214	13	-0.9726595633476407
2214	14	-0.6806695468914796
2214	15	-0.6171455983573679
2214	16	-0.5799533297431267
2214	17	-0.8948825548774622
2214	18	-0.8707511581669607
2214	19	-0.7030596799947304
2214	20	-1.0329586788764544
2214	21	-1.1697861942718504
2214	22	-1.606264401112142
2214	23	-1.5805826144767536
2214	24	-1.3723406245323062
2214	25	-1.8723660526713033
2215	1	-1.5045444852060044
2215	2	-0.9823135924406223
2215	3	-0.9456756763747798
2215	4	-0.9924067185710399
2215	5	-1.458827546955984
2215	6	-1.2793767409528662
2215	7	-1.7150270510961114
2215	8	-1.4613700729751002
2215	9	-1.3804742378178148
2215	10	-1.424335789840626
2215	11	-1.2052262359444157
2215	12	-1.3088903024209486
2215	13	-1.5456711186782783
2215	14	-1.617051969293641
2215	15	-1.4975120794639807
2215	16	-1.7180585085555435
2215	17	-1.770631121004321
2215	18	-1.503964299192603
2215	19	-1.7161121428827215
2215	20	-1.5906193570141056
2215	21	-1.2759982388062596
2215	22	-1.5161623430824491
2215	23	-2.0833132993620396
2215	24	-1.8577066839115233
2215	25	-2.1579598448440436
2216	1	-1.675022759426792
2216	2	-1.0116841781921977
2216	3	-1.0735689283324028
2216	4	-1.36389979012025
2216	5	-2.2701352200712925
2216	6	-2.196641250395519
2216	7	-2.2528897871624416
2216	8	-2.3938968463514243
2216	9	-4.299172898189379
2216	10	-2.200837114115153
2216	11	-2.390842023599877
2216	12	-2.7655390242081417
2216	13	-2.634182077529804
2216	14	-2.8919181024120206
2216	15	-2.630586315607083
2216	16	-2.1935939760820276
2216	17	-2.471544198695092
2216	18	-2.873024736011274
2216	19	-3.018218507077414
2216	20	-2.365688449598167
2216	21	-3.1183495937886043
2216	22	-2.5323851858584376
2216	23	-3.7168447940649436
2216	24	-2.9772718804415383
2216	25	-2.941655434434093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49166.0
3	1.0
4	819.0
5	6996.0
6	8518.0
7	6656.0
8	5364.0
9	4281.0
10	3782.0
11	3337.0
12	3069.0
13	2814.0
14	2675.0
15	2734.0
16	2822.0
17	2969.0
18	3340.0
19	3988.0
20	5269.0
21	7008.0
22	8817.0
23	10505.0
24	12292.0
25	14206.0
26	15388.0
27	17231.0
28	19082.0
29	22663.0
30	28796.0
31	36579.0
32	46444.0
33	59376.0
34	76349.0
35	100620.0
36	137727.0
37	212360.0
38	642989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40475276071414	18.84247706578228	13.297778753142872	26.454991420360702
2	14.356523959163992	20.37753452117919	38.5671464908327	26.69879502882412
3	17.787543469793928	24.992783919673997	29.01198310491866	28.207689505613416
4	12.162295001392598	16.316899837551247	35.743830034997686	35.77697512605847
5	13.644318653762408	38.264693593183104	33.63911308746171	14.451874665592781
6	33.097806555744	35.400828452357686	17.40601100820256	14.095353983695755
7	29.10409597742064	31.52068292984369	20.78255951138584	18.59266158134983
8	27.15748025042916	34.745438418092625	19.720571390025576	18.376509941452632
9	27.636579288021814	14.239122625468415	19.038036621134985	39.08626146537479
10	16.677330754620282	28.200156210502165	31.8390161890311	23.283496845846457
11	35.315405123887885	22.267557006828785	22.99284354542241	19.42419432386092
12	24.15945034331489	24.696681278894435	29.710521623135588	21.433346754655087
13	29.534071861570066	21.14308039371369	25.418100243235408	23.904747501480838
14	22.488998220559843	21.143958220963118	26.58920893047077	29.77783462800627
15	24.67182255541677	28.881127606986162	22.8901168015508	23.556933036046267
16	23.713981072221234	27.012415604515542	25.604931456435736	23.668671866827488
17	22.73775016383526	27.288022382416692	26.455865302213038	23.518362151535012
18	22.693540103784315	25.93820201708314	28.35303050830369	23.015227370828857
19	24.235477667353074	25.797288475643416	27.19482748625712	22.77240637074639
20	24.94179309406173	25.126038823927498	27.276811565893368	22.655356516117404
21	24.680614304320457	25.145885619121493	26.638042987188026	23.535457089370023
22	24.693388379759853	25.887699240843236	26.561413059915918	22.85749931948099
23	23.72409630051568	25.72279575963182	27.06958649857092	23.48352144128159
24	24.114762651288697	25.291109442027633	27.138772249078784	23.455355657604887
25	23.6055547819227	25.703626083707356	27.311284166829967	23.379534967539975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	116.0
2	328.5
3	541.0
4	541.0
5	541.0
6	1319.0
7	2097.0
8	2097.0
9	2097.0
10	2401.5
11	2706.0
12	2706.0
13	2706.0
14	3580.5
15	4455.0
16	4455.0
17	4455.0
18	9010.5
19	13566.0
20	13566.0
21	13566.0
22	21697.5
23	29829.0
24	29829.0
25	29829.0
26	45366.0
27	60903.0
28	60903.0
29	60903.0
30	76356.5
31	91810.0
32	91810.0
33	91810.0
34	106718.0
35	121626.0
36	121626.0
37	121626.0
38	134133.0
39	146640.0
40	146640.0
41	146640.0
42	161559.0
43	176478.0
44	176478.0
45	176478.0
46	194634.0
47	212790.0
48	212790.0
49	212790.0
50	212373.5
51	211957.0
52	211957.0
53	211957.0
54	196471.5
55	180986.0
56	180986.0
57	180986.0
58	159181.0
59	137376.0
60	137376.0
61	137376.0
62	116840.5
63	96305.0
64	96305.0
65	96305.0
66	74744.5
67	53184.0
68	53184.0
69	53184.0
70	39787.5
71	26391.0
72	26391.0
73	26391.0
74	18711.0
75	11031.0
76	11031.0
77	11031.0
78	7657.5
79	4284.0
80	4284.0
81	4284.0
82	2855.5
83	1427.0
84	1427.0
85	1427.0
86	928.0
87	429.0
88	429.0
89	429.0
90	260.0
91	91.0
92	91.0
93	91.0
94	51.5
95	12.0
96	12.0
97	12.0
98	7.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08197692296059562
2	0.0432883520937196
3	0.018777189117799766
4	0.004410749121630818
5	0.016949878767409856
6	0.05935608103680329
7	0.07302940331385882
8	0.05009350788137858
9	0.018777189117799766
10	0.04480060893542159
11	0.004599781226843567
12	3.1505350868791556E-4
13	0.005796984559857646
14	0.0010081712278013299
15	0.01499654701354478
16	0.009955690874538133
17	0.004536770525105984
18	0.0054819310511697306
19	0.001764299648652327
20	0.0013232247364892453
21	6.301070173758312E-5
22	5.040856139006649E-4
23	0.0
24	0.0
25	1.8903210521274932E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1587032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.107204678167925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32263820861286	37.47315078470834
2	14.071585504204753	14.383188010194663
3	5.213455758703827	7.993354516219492
4	2.476485508875821	5.062650071385337
5	1.3123337287191186	3.3534854239855654
6	0.843590823049426	2.5868141314926696
7	0.5421590294282647	1.9395762739575033
8	0.3875292465067065	1.5844429215995555
9	0.2726249320439741	1.2539788382108705
>10	1.4168346456068397	12.700266037125695
>50	0.08993063568655854	3.1851482447968658
>100	0.044416466597058925	4.351725014738508
>500	0.004194757823190308	1.5938696936751995
>1k	0.0019740036815013217	1.7035199586210483
>5k	2.467504601876652E-4	0.8348300792886953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7714	0.4860645532037161	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5526	0.34819713780188427	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4473	0.2818468688722093	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2725	0.17170416223491397	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2392	0.1507215985562988	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1711	0.1078113106730047	No Hit
GGATACCACGTGTCCCGCCCTACTC	1610	0.10144722979750882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.301070173758312E-5
2	0.0	0.0	0.0	0.0	6.301070173758312E-5
3	0.0	0.0	0.0	0.0	6.301070173758312E-5
4	0.0	0.0	0.0	0.0	6.301070173758312E-5
5	0.0	0.0	0.0	0.0	6.301070173758312E-5
6	0.0	0.0	0.0	0.0	6.301070173758312E-5
7	0.0	0.0	0.0	0.0	6.301070173758312E-5
8	0.0	0.0	0.0	0.0	6.301070173758312E-5
9	0.0	0.0	0.0	0.0	1.2602140347516623E-4
10	0.0	0.0	0.0	0.0	1.2602140347516623E-4
11	0.0	0.0	0.0	0.0	1.2602140347516623E-4
12	0.0	0.0	0.0	0.0	1.2602140347516623E-4
13	0.0	0.0	0.0	0.0	1.2602140347516623E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1685	0.0	13.716801	1
GTCTTAT	100	1.4200305E-7	12.364878	1
TGGTCGG	85	3.964138E-6	12.288367	10
AGATCGT	55	0.0030423878	12.10471	6
GACGTGA	475	0.0	12.006495	7
TAAGCTG	105	2.6798443E-7	11.77533	5
TCGCTTC	65	8.074323E-4	11.683525	13
CGAATTT	65	8.074323E-4	11.683525	15
TAGGACC	360	0.0	11.624364	4
TCTACAC	200	0.0	11.414814	3
TTAGGAC	625	0.0	11.414813	3
GTCCTAA	530	0.0	11.126595	1
CGGACTG	95	1.3430417E-5	11.012555	5
TAGGACG	900	0.0	10.990308	4
GGACGTG	885	0.0	10.854183	6
CCCCGGT	240	0.0	10.679135	19
CTACACT	205	0.0	10.670703	4
TTAACCT	315	0.0	10.567604	4
TTAAGCT	135	5.5910277E-8	10.567603	4
ATTGCGA	90	9.55968E-5	10.550618	10
>>END_MODULE