FastQCFastQC Report
Thu 26 May 2016
SRR1512867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471211
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61460.4177510907680816No Hit
GTCCTACAGTGGACATTTCTAAATT60410.41061411313536944No Hit
GTCCTAAAGTGTGTATTTCTCATTT55110.37458936889406075No Hit
CTGTAGGACGTGGAATATGGCAAGA53900.3663648518125544No Hit
CTTTAGGACGTGAAATATGGCGAGG43080.292819996587845No Hit
TATCAACGCAGAGTACTTTTTTTTT36620.24891059134277815No Hit
GGTATCAACGCAGAGTACTTTTTTT34690.23579214674169782No Hit
GTACTGGTTCACTATCGGTCAGTCA29220.19861189183604527No Hit
GTCCTACAGTGTGCATTTCTCATTT24600.16720919025211203No Hit
CTGAAGGACCTGGAATATGGCGAGA23970.16292700367248478No Hit
GTCCTTCAGTGTGCATTTCTCATTT21060.14314737994753982No Hit
CTGTAGGACCTGGAATATGGCGAGA18090.12295992892929702No Hit
GGATACCACGTGTCCCGCCCTACTC17170.11670657709873024No Hit
ATTTAGAAATGTCCACTGTAGGACG16610.11290018902795043No Hit
TTTCTAAATTTTCCACCTTTTTCAG16610.11290018902795043No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16420.1116087359325073No Hit
GAATATGGCAAGAAAACTGAAAATC15490.10528741288639087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1350.015.47948518
ACAACCG456.77038E-414.775375
TAATACT1001.0091753E-813.2978344
TAGCCTA500.001501321813.2978345
GGTATCA16400.012.3730281
TTACACA2250.012.2424494
TTATACT1405.2750693E-1112.2122964
TAGGACC7350.012.0184494
CTTATAC951.0416825E-611.9980453
CCAACGT802.8698967E-511.87346819
CCATTCG658.033941E-411.6904029
ATAGGAC2050.011.5834793
GGCGAGG13150.011.55744519
GTCTAGA1251.75678E-811.4319811
TCTTATA1001.9329382E-611.3981442
TTAGGCC752.0768095E-411.3981434
TAGACCC752.0768095E-411.3981434
CTTACAC2100.011.3076813
CACCTTT6350.011.21864414
TTAGACT855.3310112E-511.1746494
Produced by FastQC (version 0.11.5)