##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512867_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1471211
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.986265056473883	31.0	30.0	34.0	22.0	34.0
2	29.123328332917577	31.0	30.0	34.0	22.0	34.0
3	29.212936145800978	31.0	30.0	34.0	22.0	34.0
4	32.59069773132474	35.0	33.0	37.0	25.0	37.0
5	32.78695034226906	35.0	33.0	37.0	26.0	37.0
6	32.64463289086338	37.0	33.0	37.0	25.0	37.0
7	32.61956850512945	37.0	33.0	37.0	25.0	37.0
8	32.52476769137805	37.0	33.0	37.0	23.0	37.0
9	33.80199577083097	39.0	34.0	39.0	22.0	39.0
10	33.653736955474095	38.0	34.0	39.0	19.0	39.0
11	33.73085369807594	38.0	34.0	39.0	22.0	39.0
12	33.630666845204395	38.0	34.0	39.0	19.0	39.0
13	33.61281964313753	38.0	34.0	39.0	18.0	39.0
14	34.44501706417366	39.0	34.0	41.0	17.0	41.0
15	34.64545602228368	39.0	34.0	41.0	18.0	41.0
16	34.586800261825125	39.0	34.0	41.0	18.0	41.0
17	34.359437225523735	39.0	34.0	41.0	17.0	41.0
18	34.36820551232964	39.0	34.0	41.0	17.0	41.0
19	34.480712827731715	39.0	34.0	41.0	18.0	41.0
20	34.46003802309798	39.0	34.0	41.0	18.0	41.0
21	34.35438764392055	39.0	34.0	41.0	17.0	41.0
22	34.36965737749378	39.0	34.0	41.0	17.0	41.0
23	34.037776362466026	39.0	34.0	41.0	10.0	41.0
24	34.11020513033141	39.0	34.0	41.0	2.0	41.0
25	33.82019302465792	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6304141156894509
1101	2	0.3242652554773784
1101	3	0.7092357053977096
1101	4	0.7827446800856208
1101	5	0.5325942575590403
1101	6	0.6944990225314385
1101	7	0.30583214747056786
1101	8	0.7600426739294761
1101	9	0.7369339354159905
1101	10	0.6722906531720767
1101	11	0.7914514203264176
1101	12	0.5858805020617197
1101	13	1.0389347450952968
1101	14	1.1913233878502325
1101	15	0.9757205236001312
1101	16	0.8017928648066643
1101	17	0.2773238042750421
1101	18	0.753615947136943
1101	19	0.23281011109931882
1101	20	-0.3360325053064912
1101	21	0.6973169100514909
1101	22	0.8480830540431654
1101	23	0.4196360800683223
1101	24	0.9252119355135093
1101	25	0.6281342292726748
1102	1	0.948602614004912
1102	2	1.0579140911189029
1102	3	0.9787542593151741
1102	4	1.0668734409379752
1102	5	0.9286142879723798
1102	6	0.9289950984979569
1102	7	0.7995333404529958
1102	8	0.8735976787554804
1102	9	1.0326938803694503
1102	10	0.999804431716349
1102	11	0.7443978633005912
1102	12	0.9577430636319093
1102	13	0.8774118154946464
1102	14	1.1210931397616193
1102	15	1.110533297528697
1102	16	1.0091424056038463
1102	17	1.2043315957704976
1102	18	1.060837156697282
1102	19	1.033306382606483
1102	20	1.289306898122355
1102	21	1.2874309159886295
1102	22	1.1712877644001196
1102	23	1.1134243439809666
1102	24	0.47167159418644644
1102	25	0.9478288415129086
1103	1	0.7490116085163407
1103	2	0.8266271858159762
1103	3	0.8790158333135558
1103	4	0.917720262037534
1103	5	0.7697143986209767
1103	6	0.5524834214579428
1103	7	0.5369478207932374
1103	8	0.6711450039905102
1103	9	0.8322215629812533
1103	10	0.8534461530725892
1103	11	0.6590972098453136
1103	12	0.747485266684528
1103	13	0.8726459564328835
1103	14	0.9502372882010945
1103	15	0.8795073983937769
1103	16	0.8356966601316529
1103	17	0.5425889321878685
1103	18	0.8671083144975356
1103	19	0.7607053141846833
1103	20	0.9770009761802356
1103	21	0.7766904044831122
1103	22	0.8536569641841893
1103	23	0.0941061672803798
1103	24	0.308328725871597
1103	25	0.5298675052788084
1104	1	0.7772976093654975
1104	2	0.6624353041862641
1104	3	0.7352760937099063
1104	4	0.9746638415474038
1104	5	0.7210571003238826
1104	6	0.5961183703578357
1104	7	0.8973147361193909
1104	8	0.5772566044577516
1104	9	0.8987201650997605
1104	10	0.8538905102074494
1104	11	0.5795029382501653
1104	12	0.828648998462576
1104	13	1.0242495115204022
1104	14	1.1211997592261866
1104	15	0.8383279355282269
1104	16	0.8020453445253679
1104	17	1.1029284640535977
1104	18	0.8874917098277137
1104	19	0.6365505096835804
1104	20	0.645497879602452
1104	21	0.9737046087410661
1104	22	0.6414846895730122
1104	23	0.8294532795254881
1104	24	0.7249959206204224
1104	25	0.412648819926865
1105	1	0.6588914994990631
1105	2	0.7166508764315687
1105	3	0.8601552950722322
1105	4	0.9806091359186766
1105	5	0.8462073480968968
1105	6	0.8957201494287048
1105	7	0.9388626055459284
1105	8	0.9453176162042425
1105	9	0.6202714426204068
1105	10	0.7562411802181614
1105	11	0.9210351395930445
1105	12	1.0547188598171857
1105	13	0.9590005205106777
1105	14	1.0951947431521063
1105	15	1.0036988402399771
1105	16	0.9188034464685515
1105	17	1.309416079977403
1105	18	1.0368428567428722
1105	19	0.69639458365981
1105	20	0.9561204972245889
1105	21	0.8285438164621155
1105	22	0.7225390029278245
1105	23	1.0354805740134125
1105	24	1.174575569952701
1105	25	1.3304823013636167
1106	1	0.7373469883260277
1106	2	0.809176180780721
1106	3	0.709928109398458
1106	4	0.8737287893829233
1106	5	0.6513653238426755
1106	6	0.9001355987573376
1106	7	0.8726771344182467
1106	8	0.8743875430058239
1106	9	1.1300124896072532
1106	10	0.8429588714166627
1106	11	0.48527790672509497
1106	12	1.00467827212141
1106	13	1.022541056080847
1106	14	1.3894520234602226
1106	15	1.1165332201853175
1106	16	1.1266229911132584
1106	17	1.2663960508573382
1106	18	1.190160095561346
1106	19	1.1046177796743635
1106	20	1.3239551290127807
1106	21	1.2763004823908233
1106	22	1.1951203452384789
1106	23	1.4194835164749335
1106	24	1.2161208542247053
1106	25	1.3727866282579768
1107	1	1.024801949763802
1107	2	1.0495232995831643
1107	3	0.943805527145841
1107	4	0.9543192311391167
1107	5	0.9045005311139178
1107	6	1.0764859592364928
1107	7	0.767294767880486
1107	8	0.9873932349016741
1107	9	1.0911789243734447
1107	10	1.083418622177689
1107	11	1.1982632338526713
1107	12	1.3083956576795686
1107	13	1.0608175292367008
1107	14	1.4682259430561757
1107	15	1.0857456345783945
1107	16	1.3187469454279181
1107	17	1.5856337031522472
1107	18	1.4627337964920173
1107	19	1.3224422990829012
1107	20	1.3979998336610677
1107	21	1.1679819106702425
1107	22	1.2042295022925558
1107	23	1.4645169590650582
1107	24	1.2504995789958073
1107	25	1.4622698751074097
1108	1	0.6646504309808172
1108	2	0.6768799593302006
1108	3	0.7256214845534501
1108	4	0.7806444570704514
1108	5	0.6666805042057078
1108	6	0.7523827536104477
1108	7	0.6814354954754265
1108	8	0.7379093453090562
1108	9	0.9335625084315637
1108	10	0.9324106731294393
1108	11	0.5024054603230965
1108	12	0.749249458652514
1108	13	0.6210791205702719
1108	14	0.9471057500553854
1108	15	0.7557150739739242
1108	16	0.5335959006330029
1108	17	0.8974180482384568
1108	18	0.8277291156362381
1108	19	0.8689312279736043
1108	20	0.9064616833449577
1108	21	0.9092001637541216
1108	22	0.9687812043747783
1108	23	1.234476775608428
1108	24	1.0617351710999188
1108	25	1.2082337325332304
1109	1	0.7044310899196873
1109	2	0.4998123793229823
1109	3	0.6452452055771332
1109	4	0.717076773195636
1109	5	0.5774296058901953
1109	6	0.5900338486369918
1109	7	0.5073641857508875
1109	8	0.607422384955953
1109	9	0.7117377819839632
1109	10	0.8070099031267617
1109	11	0.7243829807550313
1109	12	0.4845571320525863
1109	13	0.5897502492043145
1109	14	0.8423366565977162
1109	15	0.590800091886365
1109	16	0.23943606017075325
1109	17	0.8313864491497611
1109	18	0.9287719031586974
1109	19	0.8112246567011354
1109	20	0.6892539925109276
1109	21	0.8347176748245033
1109	22	0.7918014225559702
1109	23	0.9296188425932357
1109	24	0.7765280526598062
1109	25	0.29898510009325463
1110	1	0.7464430518648975
1110	2	0.7583782250983155
1110	3	0.6845251863038442
1110	4	0.7492182826094833
1110	5	0.5636730490997337
1110	6	0.6988797682364094
1110	7	0.6139558699532586
1110	8	0.6968669206026519
1110	9	0.9191866015307113
1110	10	0.7739964054303883
1110	11	0.694195982974442
1110	12	0.8870911214736026
1110	13	0.9410856633703162
1110	14	0.9531441944993588
1110	15	0.8279244067146436
1110	16	0.8854502138126676
1110	17	0.943196382709516
1110	18	0.2716887572637532
1110	19	0.7000562776825774
1110	20	0.7827153951257557
1110	21	1.0913526386408918
1110	22	1.0417525642068028
1110	23	1.1155316124956371
1110	24	0.9657573273034217
1110	25	1.0790095328211677
1111	1	0.4343887943228921
1111	2	0.3895668140913884
1111	3	0.5939175087407733
1111	4	0.3767200526279595
1111	5	0.49416096980290547
1111	6	0.2857466628469183
1111	7	0.21365108140570754
1111	8	0.3660936383839939
1111	9	0.4396885960393391
1111	10	0.4388585166738892
1111	11	0.2665832630172673
1111	12	-0.36227642070464583
1111	13	0.09562986202683277
1111	14	0.19109727605835047
1111	15	0.1699718432098365
1111	16	0.3806236756234398
1111	17	-0.024423071952114128
1111	18	0.20691903235053388
1111	19	0.2098172820169637
1111	20	0.2715426931277918
1111	21	0.39907466803183667
1111	22	0.20324948178438262
1111	23	0.3313227675837922
1111	24	0.24449854196780763
1111	25	0.43564882895821455
1112	1	0.1529463548184573
1112	2	0.07583357186130968
1112	3	0.09439236295408193
1112	4	0.27375583070845977
1112	5	0.17030126451333416
1112	6	-0.19095271361148747
1112	7	-0.3266026612046815
1112	8	-0.14856560537306507
1112	9	-0.09351323920415666
1112	10	0.09265147737200152
1112	11	0.056717217743532444
1112	12	0.07825274451167274
1112	13	0.06086774035242115
1112	14	-0.07013288284232999
1112	15	-0.2733688457083687
1112	16	-0.01871403524978632
1112	17	0.2228948612073225
1112	18	-0.14557105157619077
1112	19	0.005274008688587628
1112	20	-0.21619310457715102
1112	21	-0.17152874060492707
1112	22	-0.423528893798796
1112	23	-0.11721105736331339
1112	24	-0.03939068593213335
1112	25	-0.01786290774766286
1113	1	-0.32961243926597206
1113	2	-0.10652535261933238
1113	3	-0.16229008664208067
1113	4	-0.057679453936493985
1113	5	-0.2606257955508653
1113	6	-0.567709588805215
1113	7	-0.6584918386753529
1113	8	-0.4682750284588977
1113	9	-0.6685984777116545
1113	10	-0.4752176133816235
1113	11	-0.4785330467897637
1113	12	-0.6977046522668999
1113	13	-0.6612121504852837
1113	14	-0.33926442981881877
1113	15	-0.6195845053671292
1113	16	-0.5742886514538341
1113	17	-0.4409465541972466
1113	18	-0.8494339502911146
1113	19	-1.143627955448892
1113	20	-0.9342111177806558
1113	21	-0.7409971444626535
1113	22	-1.2144987779741854
1113	23	-4.874686572446162
1113	24	-2.476324878597879
1113	25	-1.6855182342191597
1114	1	-0.40216207473320154
1114	2	-0.6070916633192738
1114	3	-0.5027922471887862
1114	4	-0.3228318343449956
1114	5	-0.43762501508906126
1114	6	-0.9086010284717077
1114	7	-0.8656882675867053
1114	8	-0.7340888978362408
1114	9	-0.88122768221168
1114	10	-0.5390974603349648
1114	11	-0.7512159318334497
1114	12	-1.63930421825399
1114	13	-0.8832544857792897
1114	14	-2.6517813239615435
1114	15	-1.2852013557278568
1114	16	-1.4164783227080946
1114	17	-1.2585659553355768
1114	18	-1.158118967328022
1114	19	-1.1542089236022406
1114	20	-0.7175254154602086
1114	21	-0.655426170027539
1114	22	-0.9156047494587725
1114	23	-5.031218657040608
1114	24	-2.288340183263518
1114	25	-2.1338364342077405
1115	1	-1.5562684221719536
1115	2	-1.5384741876503618
1115	3	-1.312054458495492
1115	4	-1.2990775630572031
1115	5	-1.2820094157358604
1115	6	-1.4430169298659763
1115	7	-1.897882717963494
1115	8	-2.284569227030495
1115	9	-3.4359939817509897
1115	10	-3.920570177484187
1115	11	-2.816342583143822
1115	12	-2.9315977853894815
1115	13	-2.401947281032058
1115	14	-3.0934479270673805
1115	15	-2.7779624763307176
1115	16	-2.799026679787751
1115	17	-2.9252977718532556
1115	18	-5.382797285966092
1115	19	-3.388801969030439
1115	20	-3.778389931736868
1115	21	-3.298633106293824
1115	22	-2.7998986140589537
1115	23	-3.18096657478781
1115	24	-2.8909828519031144
1115	25	-3.190501549990149
1116	1	-1.4354913951449255
1116	2	-1.262820108914088
1116	3	-1.6553023296543223
1116	4	-1.3392918355488739
1116	5	-1.5523213372464113
1116	6	-3.7413755624711875
1116	7	-2.2974615776450946
1116	8	-2.559670269854138
1116	9	-3.614128623241797
1116	10	-2.8667976549616654
1116	11	-2.517133185389902
1116	12	-2.179340133902379
1116	13	-3.412886902160036
1116	14	-6.671327261881647
1116	15	-4.629101714884339
1116	16	-3.2137665909317725
1116	17	-4.437042650293339
1116	18	-3.0161468170039782
1116	19	-2.8363283481492836
1116	20	-3.5103603439298823
1116	21	-4.257271110736625
1116	22	-3.743246756722165
1116	23	-2.8756336733932635
1116	24	-3.8385909908639917
1116	25	-3.6770636257645535
1201	1	0.5901957994520934
1201	2	0.7122717393271536
1201	3	0.980004376782933
1201	4	0.9544824905942235
1201	5	0.40551516682158706
1201	6	0.8373271644818772
1201	7	1.0068180047636943
1201	8	1.0260250486137252
1201	9	1.4207258139679553
1201	10	1.2287821105373453
1201	11	1.3210787142621072
1201	12	1.0787024982317703
1201	13	1.3801004997775692
1201	14	1.4711029880183162
1201	15	1.4144246513203598
1201	16	1.4339356873896207
1201	17	1.684520770462541
1201	18	1.453058256171488
1201	19	1.577026931760507
1201	20	1.6173602434138559
1201	21	1.78145974663488
1201	22	1.4544294524614472
1201	23	1.88740635182711
1201	24	1.647704524031795
1201	25	1.7496372339107324
1202	1	1.0732277797940881
1202	2	1.0258123573323168
1202	3	1.0525606271362484
1202	4	1.3544731090203612
1202	5	1.1478730931088776
1202	6	1.3193289758472915
1202	7	1.276925544061065
1202	8	1.2723358649670118
1202	9	1.7368012610661054
1202	10	1.554156140657014
1202	11	1.4697965056952853
1202	12	1.7144731163451894
1202	13	1.6448154382500775
1202	14	2.090046310081739
1202	15	1.7824445498790809
1202	16	1.6425590376458672
1202	17	2.082139534572491
1202	18	2.0878371938164904
1202	19	1.9169057170150765
1202	20	1.841015160260639
1202	21	1.9668853513554936
1202	22	1.9422941461109673
1202	23	1.981217279436244
1202	24	1.9669967684771166
1202	25	2.3065076130345687
1203	1	1.1406385364763345
1203	2	1.2390776314229583
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2211	16	-0.2715694990352375
2211	17	0.1308773091680564
2211	18	-0.17718236951655797
2211	19	0.00514926084657219
2211	20	-0.1478241280231174
2211	21	0.09777339276215002
2211	22	0.07849884031325871
2211	23	0.2513148294247074
2211	24	-0.08710754090480322
2211	25	0.2633813705413246
2212	1	-0.13697191552814658
2212	2	-0.0118218968142898
2212	3	-0.275336272727273
2212	4	-0.14012071843948348
2212	5	-0.24451616816820376
2212	6	-0.436336425606072
2212	7	-0.36847037040699604
2212	8	-0.24099713894354835
2212	9	-0.11153153408233862
2212	10	-0.4082871727692279
2212	11	-0.47573209720356147
2212	12	-0.15919420571958653
2212	13	-0.08068288073612706
2212	14	-0.10206003065556501
2212	15	-0.4099613357568188
2212	16	-0.4191146522378304
2212	17	-0.07308447866264345
2212	18	0.09596282800322342
2212	19	-0.16967654847133673
2212	20	-0.13374090408712647
2212	21	-0.13228124731983826
2212	22	0.05806250367410826
2212	23	0.0785613274369723
2212	24	-0.06521120301785288
2212	25	-0.07401878924350314
2213	1	-0.6399935626821147
2213	2	-0.44514153539893897
2213	3	-0.7083275338468447
2213	4	-0.4333238052617787
2213	5	-0.42412641080245805
2213	6	-0.2309591148952066
2213	7	-0.41528621161758394
2213	8	-0.3312568369659985
2213	9	-0.7799440495630634
2213	10	-0.5640931995334739
2213	11	-0.7046812392803687
2213	12	-0.5314819644488793
2213	13	-0.6600930577319488
2213	14	-0.7895304845595774
2213	15	-0.6986177289528825
2213	16	-0.625702067264136
2213	17	-0.6164761694155771
2213	18	-0.5671482074995353
2213	19	-0.6863879408158837
2213	20	-0.41692254815745144
2213	21	-0.548629589838292
2213	22	-0.721020444807138
2213	23	-0.7888340332862285
2213	24	-0.41323664819447714
2213	25	-0.29017751786677337
2214	1	-2.6234455744898355
2214	2	-1.460436599576937
2214	3	-1.5685503458824712
2214	4	-3.3851572373745427
2214	5	-1.7703841019979158
2214	6	-1.4267433806204615
2214	7	-1.344615685395226
2214	8	-1.1876727756712135
2214	9	-1.0037742291125653
2214	10	-1.0163621200890418
2214	11	-0.9511854495746164
2214	12	-0.9692363214806861
2214	13	-1.3458473460476341
2214	14	-1.3709364737425176
2214	15	-1.6661430608660837
2214	16	-1.4536597953945147
2214	17	-3.4909833459558897
2214	18	-3.2787116997675376
2214	19	-2.123372677246593
2214	20	-2.055858822540941
2214	21	-1.9501384814185627
2214	22	-2.9083234227922077
2214	23	-1.9378495316356137
2214	24	-3.050174428606372
2214	25	-3.024268446479308
2215	1	-1.8746396342931675
2215	2	-3.5992714953654747
2215	3	-2.140156664508659
2215	4	-3.6162486625057007
2215	5	-2.2331976681227417
2215	6	-1.9542397253353485
2215	7	-2.1700192002027165
2215	8	-1.7072249674088376
2215	9	-4.179537538650713
2215	10	-2.327631865795876
2215	11	-1.9834781017348178
2215	12	-2.0699157744780194
2215	13	-2.0156188548919864
2215	14	-1.7896085301629157
2215	15	-1.8783646141472374
2215	16	-2.0300939572591403
2215	17	-2.2358960491467386
2215	18	-1.9981463490035125
2215	19	-2.0420906039115394
2215	20	-1.9467707803403158
2215	21	-2.678781582247094
2215	22	-2.4150204784958866
2215	23	-2.359113256872849
2215	24	-2.2773238795968815
2215	25	-3.2185518672527955
2216	1	-2.834270308173629
2216	2	-2.8766957196463636
2216	3	-4.104758617459311
2216	4	-4.605911304976392
2216	5	-4.2780400568041905
2216	6	-3.3360430296030295
2216	7	-4.992315568266058
2216	8	-2.722915703049278
2216	9	-3.417629148075097
2216	10	-2.469580026604053
2216	11	-2.670940681066636
2216	12	-2.97125656835863
2216	13	-2.5899177049661937
2216	14	-3.5935035454577644
2216	15	-3.0518285070579054
2216	16	-3.358503909977557
2216	17	-3.010891604024941
2216	18	-2.6644915154324984
2216	19	-3.2633451805726565
2216	20	-4.066642107671829
2216	21	-3.4221235155303518
2216	22	-3.0962333980155954
2216	23	-2.900710888076514
2216	24	-3.6750374540916795
2216	25	-3.5606767443315697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72580.0
3	5.0
4	661.0
5	4027.0
6	5136.0
7	4680.0
8	4201.0
9	3649.0
10	3360.0
11	3031.0
12	2934.0
13	2812.0
14	2798.0
15	2832.0
16	2910.0
17	3183.0
18	3625.0
19	4326.0
20	5562.0
21	7472.0
22	9126.0
23	11024.0
24	12805.0
25	14344.0
26	15599.0
27	17379.0
28	19439.0
29	23099.0
30	27998.0
31	34437.0
32	43493.0
33	55555.0
34	71376.0
35	94528.0
36	127841.0
37	196566.0
38	556818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43977632111111	18.539580646415345	12.718241396690377	24.302401635783166
2	14.65093024315004	19.53303282583322	42.81953167326845	22.996505257748286
3	19.495225593835247	25.55984645221549	28.563368371250103	26.38155958269916
4	12.496286673559688	16.137080037225036	37.25283861288479	34.11379467633049
5	12.958190547989812	39.142682921026136	34.39193327672904	13.507193254255009
6	32.06641637171182	35.79560258204258	18.225522602396328	13.912458443849282
7	28.98543626247516	31.70560148460464	20.976775161715377	18.33218709120482
8	25.99768652400231	37.09454632055251	20.03000714455823	16.87776001088695
9	28.237082893975984	14.35144052518793	19.93612027619987	37.475356304636215
10	17.44206694184345	28.07667213437081	32.51170363093201	21.969557292853725
11	34.515378492560764	21.90637522025158	24.84444625861447	18.733800028573178
12	25.431434504418444	24.172579244877355	30.540949877121793	19.85503637358241
13	30.01403440504123	22.19490475584693	25.370435501942573	22.420625337169266
14	21.306904961952807	23.375910360334313	26.638342455362153	28.678842222350724
15	23.35631852357796	32.51551772462944	22.654925533970278	21.47323821782232
16	21.586364916586955	27.549502896087795	28.89189435572421	21.97223783160103
17	21.40003320387451	29.127146344716092	27.757894765490832	21.714925685918562
18	21.39276706087598	26.145120103314095	31.466843755804785	20.995269080005144
19	24.052492316861674	26.188734753639498	27.3843583074167	22.374414622082124
20	24.901280428896055	26.31594344885768	28.15766556449089	20.62511055775537
21	23.41289196828539	26.105368422126755	26.845035833698983	23.636703775888872
22	24.472232768721398	28.451597012142766	26.55479212509917	20.52137809403667
23	22.191212290274734	28.03739335138592	28.321177319054552	21.45021703928479
24	24.220663473575353	26.00874925161922	28.613794698742723	21.1567925760627
25	22.724232872770123	27.253894399969518	28.508905907813425	21.51296681944693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	258.0
2	544.5
3	831.0
4	831.0
5	831.0
6	1504.0
7	2177.0
8	2177.0
9	2177.0
10	2364.5
11	2552.0
12	2552.0
13	2552.0
14	3299.5
15	4047.0
16	4047.0
17	4047.0
18	8322.0
19	12597.0
20	12597.0
21	12597.0
22	21713.5
23	30830.0
24	30830.0
25	30830.0
26	49437.0
27	68044.0
28	68044.0
29	68044.0
30	91434.0
31	114824.0
32	114824.0
33	114824.0
34	127419.5
35	140015.0
36	140015.0
37	140015.0
38	147241.0
39	154467.0
40	154467.0
41	154467.0
42	164021.5
43	173576.0
44	173576.0
45	173576.0
46	188362.0
47	203148.0
48	203148.0
49	203148.0
50	193825.5
51	184503.0
52	184503.0
53	184503.0
54	162025.5
55	139548.0
56	139548.0
57	139548.0
58	120164.0
59	100780.0
60	100780.0
61	100780.0
62	85223.0
63	69666.0
64	69666.0
65	69666.0
66	53915.0
67	38164.0
68	38164.0
69	38164.0
70	28535.0
71	18906.0
72	18906.0
73	18906.0
74	13394.5
75	7883.0
76	7883.0
77	7883.0
78	5441.0
79	2999.0
80	2999.0
81	2999.0
82	1976.5
83	954.0
84	954.0
85	954.0
86	646.0
87	338.0
88	338.0
89	338.0
90	207.5
91	77.0
92	77.0
93	77.0
94	46.0
95	15.0
96	15.0
97	15.0
98	13.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3399920201792945
2	0.009787855039148021
3	0.009380027745850186
4	0.010739452056842968
5	0.012234818798935026
6	0.08238111324616251
7	0.08088574650407046
8	0.10610306747298655
9	0.08571170280809483
10	0.0929846228719062
11	0.0884305514300804
12	0.09944188834912192
13	0.08509996186814808
14	0.09012983181882137
15	0.08693518468798833
16	0.08815866656788184
17	0.10216073697110747
18	0.10318030520435205
19	0.09747072309818239
20	0.0945479608295479
21	0.08333271026385747
22	0.10392798857539809
23	0.09726680945153347
24	0.09318853651855512
25	0.09135331369871487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1471211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0454952703563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09016258025511	39.60150196691309
2	13.282766385021988	13.826163101378189
3	4.586251410997493	7.160811783592049
4	2.091479798844005	4.354084079151256
5	1.0999882823701899	2.862471747377253
6	0.6801608605553267	2.1239585310668234
7	0.4265574992719783	1.554027741762633
8	0.3004412957821073	1.2509292830917906
9	0.22881385424072323	1.0717857331809801
>10	1.0700081619783808	9.72225619317631
>50	0.07461996025381837	2.6826142904743624
>100	0.05671876332815727	5.814573801213201
>500	0.007535993238928555	2.8504360845858434
>1k	0.00396631223101503	3.5357105074317197
>5k	5.288416308020039E-4	1.5886751556045373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6146	0.4177510907680816	No Hit
GTCCTACAGTGGACATTTCTAAATT	6041	0.41061411313536944	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5511	0.37458936889406075	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5390	0.3663648518125544	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4308	0.292819996587845	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3662	0.24891059134277815	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3469	0.23579214674169782	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2922	0.19861189183604527	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2460	0.16720919025211203	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2397	0.16292700367248478	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2106	0.14314737994753982	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1809	0.12295992892929702	No Hit
GGATACCACGTGTCCCGCCCTACTC	1717	0.11670657709873024	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1661	0.11290018902795043	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1661	0.11290018902795043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1642	0.1116087359325073	No Hit
GAATATGGCAAGAAAACTGAAAATC	1549	0.10528741288639087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.797121554963904E-5	0.0
12	0.0	0.0	0.0	6.797121554963904E-5	0.0
13	0.0	0.0	0.0	6.797121554963904E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	135	0.0	15.479485	18
ACAACCG	45	6.77038E-4	14.77537	5
TAATACT	100	1.0091753E-8	13.297834	4
TAGCCTA	50	0.0015013218	13.297834	5
GGTATCA	1640	0.0	12.373028	1
TTACACA	225	0.0	12.242449	4
TTATACT	140	5.2750693E-11	12.212296	4
TAGGACC	735	0.0	12.018449	4
CTTATAC	95	1.0416825E-6	11.998045	3
CCAACGT	80	2.8698967E-5	11.873468	19
CCATTCG	65	8.033941E-4	11.690402	9
ATAGGAC	205	0.0	11.583479	3
GGCGAGG	1315	0.0	11.557445	19
GTCTAGA	125	1.75678E-8	11.431981	1
TCTTATA	100	1.9329382E-6	11.398144	2
TTAGGCC	75	2.0768095E-4	11.398143	4
TAGACCC	75	2.0768095E-4	11.398143	4
CTTACAC	210	0.0	11.307681	3
CACCTTT	635	0.0	11.218644	14
TTAGACT	85	5.3310112E-5	11.174649	4
>>END_MODULE