FastQCFastQC Report
Thu 26 May 2016
SRR1512867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471211
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76360.5190282019370437No Hit
GTCCTACAGTGGACATTTCTAAATT58160.39532058963670064No Hit
TATCAACGCAGAGTACTTTTTTTTT56960.387164043770744No Hit
CTGTAGGACGTGGAATATGGCAAGA53430.3631702046817214No Hit
GTCCTAAAGTGTGTATTTCTCATTT50890.3459055159321131No Hit
CTTTAGGACGTGAAATATGGCGAGG45520.3094049731819569No Hit
GGTATCAACGCAGAGTACTTTTTTT44370.30158828339374844No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26300.17876429689555065No Hit
GTACTGGTTCACTATCGGTCAGTCA26150.1777447286623061No Hit
CTGAAGGACCTGGAATATGGCGAGA25500.17332659965157957No Hit
GTCCTACAGTGTGCATTTCTCATTT22570.1534110334955353No Hit
TTTCTAAATTTTCCACCTTTTTCAG18420.12520297904243513No Hit
GTCCTTCAGTGTGCATTTCTCATTT17870.12146456218720496No Hit
ATTTAGAAATGTCCACTGTAGGACG17750.1206489076006093No Hit
CTGTAGGACCTGGAATATGGCGAGA17560.11935745450516616No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16520.1122884480880037No Hit
GGATACCACGTGTCCCGCCCTACTC16120.10956959946601813No Hit
ATCATTAACTGAATCCATAGGTTAA15670.10651089476628438No Hit
ATTCCAGGTCCTTCAGTGTGCATTT14850.10093725509121398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG508.7547574E-515.19157114
TATAGCG400.005298387314.24209816
ACCGTGC500.001498833513.3007628
TAGGACC6700.013.0544024
GGTCTAT604.0559116E-412.6798971
ATACGGC604.1109856E-412.65964215
TATACAC6150.011.9047073
CGTTTTG802.8834342E-511.868010518
GGTATCA16600.011.7441831
AGGACCT15400.011.4824935
TATGTCG1251.8459104E-811.39367916
GGCGAGG12100.011.220283519
GATATAC4200.011.0949121
ACCGATG600.005872899711.0839678
ACCACGA600.005872899711.0839678
CACCTTT6950.010.92918814
ACCATTC700.001490607210.8577658
CCAACGA700.001499086710.85038519
TAGACTA1158.730058E-710.7470565
TGGCGAG25000.010.70969418
Produced by FastQC (version 0.11.5)