##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1471211 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.185861851223244 31.0 30.0 34.0 26.0 34.0 2 30.22045648108939 31.0 30.0 34.0 26.0 34.0 3 30.394472988578798 31.0 30.0 34.0 26.0 34.0 4 33.97076490047994 37.0 35.0 37.0 30.0 37.0 5 33.54343190745583 37.0 35.0 37.0 28.0 37.0 6 33.39337661287198 37.0 35.0 37.0 27.0 37.0 7 33.22377279669605 37.0 35.0 37.0 26.0 37.0 8 33.16111353164162 37.0 35.0 37.0 26.0 37.0 9 34.464027253738585 39.0 35.0 39.0 26.0 39.0 10 34.39027372688214 39.0 35.0 39.0 26.0 39.0 11 34.3579486559032 39.0 35.0 39.0 26.0 39.0 12 34.228746250537824 39.0 35.0 39.0 25.0 39.0 13 34.212578617207186 39.0 35.0 39.0 25.0 39.0 14 35.27096861021295 40.0 35.0 41.0 25.0 41.0 15 35.281840606140115 40.0 35.0 41.0 25.0 41.0 16 35.284069382297986 40.0 36.0 41.0 25.0 41.0 17 35.289508438966266 40.0 36.0 41.0 25.0 41.0 18 35.21507519995432 40.0 35.0 41.0 25.0 41.0 19 35.22511590791532 40.0 35.0 41.0 25.0 41.0 20 35.20741076568895 40.0 35.0 41.0 25.0 41.0 21 35.175994469861905 40.0 35.0 41.0 25.0 41.0 22 35.13266553879763 40.0 35.0 41.0 24.0 41.0 23 34.97415054672647 40.0 35.0 41.0 24.0 41.0 24 34.93724693466811 40.0 35.0 41.0 23.0 41.0 25 34.86805903436013 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6073600495567533 1101 2 0.4350611839873899 1101 3 0.38931055445549134 1101 4 0.29905354820303387 1101 5 0.4568985234744787 1101 6 0.5862593739333661 1101 7 0.5496691952017443 1101 8 0.3787010961554671 1101 9 0.5673876021783428 1101 10 0.34029596099853876 1101 11 0.5613038902884284 1101 12 0.5562859779561649 1101 13 0.7396145662162255 1101 14 0.7995640015002579 1101 15 0.4599598821955979 1101 16 0.6457800760416319 1101 17 0.8066534006326336 1101 18 0.6054570482942552 1101 19 0.5382506987194446 1101 20 0.634226003721686 1101 21 0.6669432679918543 1101 22 0.5604031486423082 1101 23 0.6439613582167709 1101 24 0.6533822216840264 1101 25 0.6267437088688723 1102 1 0.8560158100838215 1102 2 0.8818902177152843 1102 3 0.48282653723889624 1102 4 0.6654368722341744 1102 5 0.7742626355344342 1102 6 0.7699386577544729 1102 7 0.9188737157571865 1102 8 0.9212411281839579 1102 9 1.0668076417333054 1102 10 0.9195638149973178 1102 11 1.0757098453825407 1102 12 1.0543815063268838 1102 13 1.1247113762595546 1102 14 1.1167184300347657 1102 15 1.1696961872275295 1102 16 1.2304787948404439 1102 17 1.372435918847735 1102 18 0.8489701307630142 1102 19 1.2050531233415 1102 20 1.2170779497303315 1102 21 1.2051324181094785 1102 22 1.0889689932662066 1102 23 1.3431972944065649 1102 24 1.371405637779155 1102 25 1.3363316161760466 1103 1 0.6303142018119807 1103 2 0.7715212239165794 1103 3 0.5084468478656063 1103 4 0.35094519496311705 1103 5 0.5857819166063578 1103 6 0.5651451512276537 1103 7 0.6644886457016028 1103 8 0.7333131627534755 1103 9 0.712342664237994 1103 10 0.6557572642320082 1103 11 0.7808494347315289 1103 12 0.8948221505284835 1103 13 0.9022160515594635 1103 14 0.9775933313252878 1103 15 0.9523181890097305 1103 16 0.9673227035576062 1103 17 0.9096073677883325 1103 18 0.8587599180008922 1103 19 0.8969311322305913 1103 20 0.8711437424734214 1103 21 0.9363029181859375 1103 22 0.9066461010280733 1103 23 1.0436990802330897 1103 24 1.0160795161526295 1103 25 0.8892182601986818 1104 1 0.5056589674519998 1104 2 0.6703570043826481 1104 3 0.5470836431327584 1104 4 0.4746306784453793 1104 5 0.5681566463963179 1104 6 0.47365040410891623 1104 7 0.33433475103805677 1104 8 0.5227345440896656 1104 9 0.6684020037742755 1104 10 0.5076926277436087 1104 11 0.6493350852029707 1104 12 0.5289866083714898 1104 13 0.41112940633485096 1104 14 0.7014424626685383 1104 15 0.6880103789434102 1104 16 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-2.482481350203635 2216 19 -2.680903253873062 2216 20 -1.9691189252217 2216 21 -2.7042138990068523 2216 22 -2.2147086304484276 2216 23 -3.2253458710812595 2216 24 -2.5901392457100982 2216 25 -2.6335047113366485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42534.0 3 0.0 4 710.0 5 6211.0 6 8017.0 7 5998.0 8 4916.0 9 4137.0 10 3451.0 11 3114.0 12 2753.0 13 2499.0 14 2445.0 15 2437.0 16 2515.0 17 2730.0 18 3018.0 19 3607.0 20 4871.0 21 6308.0 22 8045.0 23 9502.0 24 11195.0 25 12769.0 26 14154.0 27 15482.0 28 17324.0 29 20822.0 30 26347.0 31 32663.0 32 41470.0 33 53314.0 34 69229.0 35 90964.0 36 125095.0 37 194130.0 38 616435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.497989672837114 19.48200910259812 13.790912368784483 26.229088855780287 2 13.216153492037696 20.1053027402784 41.521483839776096 25.15705992790781 3 17.639296696065042 25.39085471020971 29.521742174917726 27.448106418807527 4 11.637467805119776 16.725567408211027 36.54979644746618 35.08716833920302 5 12.609827131890455 39.21804169827893 34.471306939470324 13.700824230360285 6 32.1950058320836 36.20183430081309 17.74524506653336 13.857914800569946 7 28.613000059179605 31.783122384108303 21.041071327752313 18.56280622895978 8 25.873342622873896 37.40474990666256 19.740953748832858 16.980953721630687 9 27.452128144150613 14.227557709330169 19.926494283115304 38.39381986340392 10 16.825559388747553 28.558187106465944 32.51258594929986 22.103667555486645 11 34.20117257201122 21.887603024895913 24.849078086498427 19.062146316594443 12 24.88730318840895 24.419387114227533 30.625460507176435 20.067849190187083 13 29.796611713332705 22.02026065730559 25.67453387776854 22.508593751593168 14 20.95978597107265 23.176747192603603 26.90077440026971 28.962692436054034 15 23.197714218899026 32.46660281018377 22.58534459727248 21.75033837364472 16 21.503618434916437 27.460678495633818 28.76931066877487 22.266392400674878 17 21.109340003874532 29.183119271043985 27.92967382548967 21.777866899591814 18 21.207336164277223 26.12684323472612 31.671179092434826 20.994641508561827 19 24.002022871498717 25.922119447299302 27.74372338863603 22.332134292565947 20 24.53612381813362 26.211298336720613 28.51793446121847 20.7346433839273 21 23.030969150479265 25.785376079216643 27.38644350281334 23.797211267490752 22 24.04951869287834 28.191234106533297 26.93450661431945 20.824740586268913 23 21.82093527033172 27.774398097893503 28.710905505736427 21.693761126038343 24 24.08063833127947 25.81444809751966 28.926782086322085 21.178131484878783 25 22.380625471550626 27.211818842993186 28.88921364047281 21.51834204498338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 92.0 1 92.0 2 312.5 3 533.0 4 533.0 5 533.0 6 1252.0 7 1971.0 8 1971.0 9 1971.0 10 2122.5 11 2274.0 12 2274.0 13 2274.0 14 3235.0 15 4196.0 16 4196.0 17 4196.0 18 8486.0 19 12776.0 20 12776.0 21 12776.0 22 22497.0 23 32218.0 24 32218.0 25 32218.0 26 51696.0 27 71174.0 28 71174.0 29 71174.0 30 93274.0 31 115374.0 32 115374.0 33 115374.0 34 128230.0 35 141086.0 36 141086.0 37 141086.0 38 147592.5 39 154099.0 40 154099.0 41 154099.0 42 164538.5 43 174978.0 44 174978.0 45 174978.0 46 189072.0 47 203166.0 48 203166.0 49 203166.0 50 193714.5 51 184263.0 52 184263.0 53 184263.0 54 161584.0 55 138905.0 56 138905.0 57 138905.0 58 119202.5 59 99500.0 60 99500.0 61 99500.0 62 84013.0 63 68526.0 64 68526.0 65 68526.0 66 52505.5 67 36485.0 68 36485.0 69 36485.0 70 27273.0 71 18061.0 72 18061.0 73 18061.0 74 12811.5 75 7562.0 76 7562.0 77 7562.0 78 5179.5 79 2797.0 80 2797.0 81 2797.0 82 1839.5 83 882.0 84 882.0 85 882.0 86 559.0 87 236.0 88 236.0 89 236.0 90 143.5 91 51.0 92 51.0 93 51.0 94 28.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0884305514300804 2 0.04037490203648559 3 0.01821628576730326 4 0.006253351830566792 5 0.017876429689555065 6 0.06090220913247658 7 0.07544804926009933 8 0.05029869950673289 9 0.02073122074263991 10 0.04907521762683939 11 0.005845524537268958 12 4.7579850884747326E-4 13 0.005709582106169679 14 5.437697243971123E-4 15 0.013866127972126364 16 0.009040171668101993 17 0.004486100226276176 18 0.005981466968368236 19 0.002378992544237366 20 0.0014273955265424198 21 3.398560777481952E-4 22 6.117409399467513E-4 23 0.0 24 0.0 25 6.797121554963904E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1471211.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.26520453810699 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56773556072312 38.73995420002051 2 13.688099751097305 14.034464669560293 3 4.581941171113407 7.046824539561067 4 2.0703107495861324 4.245396161398986 5 1.150230650996538 2.9483404794668737 6 0.7162673600718583 2.2031755630832244 7 0.4540944325414275 1.6295470774696335 8 0.3025486783041052 1.2408175900795106 9 0.23597590940139385 1.088761793537543 >10 1.0870467075857213 9.844720703102796 >50 0.07696138854303623 2.7712710058676207 >100 0.05665656683338222 5.836131005945383 >500 0.0071986588235325 2.6864797379288268 >1k 0.004265871895426667 3.6625924343632565 >5k 6.665424836604167E-4 2.021523038614467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7636 0.5190282019370437 No Hit GTCCTACAGTGGACATTTCTAAATT 5816 0.39532058963670064 No Hit TATCAACGCAGAGTACTTTTTTTTT 5696 0.387164043770744 No Hit CTGTAGGACGTGGAATATGGCAAGA 5343 0.3631702046817214 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5089 0.3459055159321131 No Hit CTTTAGGACGTGAAATATGGCGAGG 4552 0.3094049731819569 No Hit GGTATCAACGCAGAGTACTTTTTTT 4437 0.30158828339374844 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2630 0.17876429689555065 No Hit GTACTGGTTCACTATCGGTCAGTCA 2615 0.1777447286623061 No Hit CTGAAGGACCTGGAATATGGCGAGA 2550 0.17332659965157957 No Hit GTCCTACAGTGTGCATTTCTCATTT 2257 0.1534110334955353 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1842 0.12520297904243513 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1787 0.12146456218720496 No Hit ATTTAGAAATGTCCACTGTAGGACG 1775 0.1206489076006093 No Hit CTGTAGGACCTGGAATATGGCGAGA 1756 0.11935745450516616 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1652 0.1122884480880037 No Hit GGATACCACGTGTCCCGCCCTACTC 1612 0.10956959946601813 No Hit ATCATTAACTGAATCCATAGGTTAA 1567 0.10651089476628438 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1485 0.10093725509121398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 6.797121554963904E-5 0.0 12 0.0 0.0 0.0 6.797121554963904E-5 0.0 13 0.0 0.0 0.0 6.797121554963904E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACGG 50 8.7547574E-5 15.191571 14 TATAGCG 40 0.0052983873 14.242098 16 ACCGTGC 50 0.0014988335 13.300762 8 TAGGACC 670 0.0 13.054402 4 GGTCTAT 60 4.0559116E-4 12.679897 1 ATACGGC 60 4.1109856E-4 12.659642 15 TATACAC 615 0.0 11.904707 3 CGTTTTG 80 2.8834342E-5 11.8680105 18 GGTATCA 1660 0.0 11.744183 1 AGGACCT 1540 0.0 11.482493 5 TATGTCG 125 1.8459104E-8 11.393679 16 GGCGAGG 1210 0.0 11.2202835 19 GATATAC 420 0.0 11.094912 1 ACCGATG 60 0.0058728997 11.083967 8 ACCACGA 60 0.0058728997 11.083967 8 CACCTTT 695 0.0 10.929188 14 ACCATTC 70 0.0014906072 10.857765 8 CCAACGA 70 0.0014990867 10.850385 19 TAGACTA 115 8.730058E-7 10.747056 5 TGGCGAG 2500 0.0 10.709694 18 >>END_MODULE