FastQCFastQC Report
Thu 26 May 2016
SRR1512866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852233
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100890.5446938911033331No Hit
TATCAACGCAGAGTACTTTTTTTTT60680.3276045724269031No Hit
GGTATCAACGCAGAGTACTTTTTTT57640.3111919504727537No Hit
GTCCTACAGTGGACATTTCTAAATT54560.29456337296657603No Hit
GTCCTAAAGTGTGTATTTCTCATTT47960.25893070688190956No Hit
CTGTAGGACGTGGAATATGGCAAGA43780.23636335169495412No Hit
CTTTAGGACGTGAAATATGGCGAGG39970.21579358536426033No Hit
GTCCTACAGTGTGCATTTCTCATTT37100.20029877450623113No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29320.15829541963673036No Hit
CTGTAGGACCTGGAATATGGCGAGA27130.14647185316318195No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23180.12514624240038916No Hit
GTACTGGTTCACTATCGGTCAGTCA21930.11839763139950535No Hit
CTGAAGGACCTGGAATATGGCGAGA20170.10889558711026097No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19110.1031727649815115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.013.27776051
TAGGACC7850.013.0683544
TGGACCG802.0029293E-613.0607915
TCCAACG2050.012.97426118
TGCACCG752.0767456E-411.3985075
CCAACGA1354.7366484E-911.25809119
CTATACC1803.6379788E-1211.0818824
GTCCTAC28400.010.99982551
ATAGGAC2600.010.9601043
GCGTGTA700.00149427410.8548429
TAGAAAT7050.010.7787314
TAGGACT3450.010.7377254
TGTAGGA28900.010.7148612
GCACCGT803.7759705E-410.6861016
GGACCGT803.7759705E-410.6861016
AGGACCT14800.010.6540115
GTCCTAA14550.010.6043071
GGCGAGG12450.010.52902719
CTGTAGG28150.010.4546721
CCAACGT1002.4018018E-510.44891519
Produced by FastQC (version 0.11.5)