FastQCFastQC Report
Thu 26 May 2016
SRR1512866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852233
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT128160.691921588698614No Hit
TATCAACGCAGAGTACTTTTTTTTT96470.520830802604208No Hit
GGTATCAACGCAGAGTACTTTTTTT75550.40788604889341673No Hit
GTCCTACAGTGGACATTTCTAAATT51870.2800403620926741No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46130.24905074037661568No Hit
CTGTAGGACGTGGAATATGGCAAGA43540.23506761838278448No Hit
GTCCTAAAGTGTGTATTTCTCATTT42990.2320982295423956No Hit
CTTTAGGACGTGAAATATGGCGAGG42910.23166631843833901No Hit
GTCCTACAGTGTGCATTTCTCATTT33680.18183457480781307No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28310.15284254194801625No Hit
CTGTAGGACCTGGAATATGGCGAGA27320.1474976420353163No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23740.12816962012878508No Hit
CTGAAGGACCTGGAATATGGCGAGA20970.1132146981508266No Hit
GTACTGGTTCACTATCGGTCAGTCA19450.1050083871737519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG359.8616954E-519.0198255
GGTATCA19600.013.684851
GGCGTGC1352.7284841E-1112.6672078
AGCACCG1001.4235229E-712.3628855
GGCGAGG13000.011.90175619
GTCTAAG1603.6379788E-1211.8893191
TAGGACC10100.011.8638514
CACCTTT6600.011.50662614
AGGACCG752.0542281E-411.4118955
TCCAACG2250.011.39094518
TGGCGAG28300.011.27019418
CGTGCGC1104.9859227E-711.22441610
GATATAC7150.011.1742971
TTAGGAC18150.011.10953
GCGTGCA600.00588633511.0808129
TGTCGAG1201.2896453E-711.07453118
GAACAGT5250.011.0493146
GCGTGCG1303.277637E-810.9590459
CGCCCTA2950.010.94075116
CCACCTT6600.010.93129513
Produced by FastQC (version 0.11.5)