##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512866_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1852233
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.137023257873064	31.0	30.0	34.0	26.0	34.0
2	30.172233730853517	31.0	30.0	34.0	26.0	34.0
3	30.331517687029656	31.0	30.0	34.0	26.0	34.0
4	33.88245107391997	37.0	35.0	37.0	30.0	37.0
5	33.48922408789823	37.0	35.0	37.0	28.0	37.0
6	33.34172752564067	37.0	35.0	37.0	27.0	37.0
7	33.16321056800089	37.0	35.0	37.0	26.0	37.0
8	33.095335737998404	37.0	35.0	37.0	26.0	37.0
9	34.37882167092369	39.0	35.0	39.0	25.0	39.0
10	34.298588244567505	39.0	35.0	39.0	25.0	39.0
11	34.27166722545166	39.0	35.0	39.0	25.0	39.0
12	34.14981268555306	39.0	35.0	39.0	25.0	39.0
13	34.13263396127809	39.0	35.0	39.0	25.0	39.0
14	35.188800221138486	40.0	35.0	41.0	25.0	41.0
15	35.20002127162188	40.0	35.0	41.0	25.0	41.0
16	35.199967282733866	40.0	35.0	41.0	25.0	41.0
17	35.21120506977254	40.0	36.0	41.0	25.0	41.0
18	35.14920099145194	40.0	35.0	41.0	25.0	41.0
19	35.18089246871209	40.0	35.0	41.0	24.0	41.0
20	35.16195370668809	40.0	35.0	41.0	24.0	41.0
21	35.13018232587369	40.0	35.0	41.0	24.0	41.0
22	35.093563282805135	40.0	35.0	41.0	24.0	41.0
23	34.937566710019745	40.0	35.0	41.0	23.0	41.0
24	34.90813034861165	40.0	35.0	41.0	22.0	41.0
25	34.843427365779576	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7049233085653235
1101	2	0.5609160100255508
1101	3	0.4445206082773083
1101	4	0.43072730689004146
1101	5	0.5972329550163167
1101	6	0.5674333448313718
1101	7	0.6118036134855629
1101	8	0.4358933193203285
1101	9	0.7521588005829329
1101	10	0.4380335552591248
1101	11	0.770724936851451
1101	12	0.883771952267729
1101	13	0.8735766226365484
1101	14	1.0835819299795375
1101	15	0.819348744931716
1101	16	0.9906477778551803
1101	17	1.0470552320964117
1101	18	0.930240581675065
1101	19	0.8862377166268232
1101	20	0.9422950688377796
1101	21	0.9899771966239754
1101	22	0.8427661243329183
1101	23	0.9586938671056018
1101	24	1.0764651547139081
1101	25	1.1147720217737955
1102	1	0.8701469687933532
1102	2	0.9883575730996128
1102	3	0.6652529614024694
1102	4	0.7524130876320143
1102	5	0.9510174416485597
1102	6	0.893427731180978
1102	7	0.9529218119602874
1102	8	0.9036199628148509
1102	9	1.0016948960780212
1102	10	0.8456640322809292
1102	11	1.0057595398776442
1102	12	1.1481372339697415
1102	13	1.0312336016655763
1102	14	0.9530474871591679
1102	15	1.050200209060172
1102	16	1.0786758661627331
1102	17	1.2203079261090082
1102	18	0.6194936834843148
1102	19	0.8761473758205653
1102	20	1.034992882446062
1102	21	0.9876540927470856
1102	22	0.7182327148520784
1102	23	0.9970953949582579
1102	24	0.8425226253909912
1102	25	0.9852838113469033
1103	1	0.8276556283614269
1103	2	0.9098466332676445
1103	3	0.7535206343766738
1103	4	0.6257545080287841
1103	5	0.7426588507578202
1103	6	0.7766729011231135
1103	7	0.8922153017580925
1103	8	0.9115712741407762
1103	9	0.926287442323158
1103	10	0.8615668871861928
1103	11	1.0851261012406184
1103	12	1.1025190659938602
1103	13	1.1128004156273548
1103	14	1.1694829223393342
1103	15	1.1483171681570994
1103	16	1.1379312571125624
1103	17	1.2449358445471859
1103	18	1.1787990089154547
1103	19	1.0859572020903698
1103	20	1.1550209758175072
1103	21	1.3005212563431314
1103	22	1.1705130019730206
1103	23	1.2578538370210168
1103	24	1.3845184531908856
1103	25	1.2046925545726523
1104	1	0.798505793384706
1104	2	0.9450683667221149
1104	3	0.8939185723128631
1104	4	0.8037496933527493
1104	5	0.9424206834144613
1104	6	0.6938851113057964
1104	7	0.7073180541992201
1104	8	0.7775703194182384
1104	9	0.7880935788963868
1104	10	0.8298820756583467
1104	11	0.8628636141370691
1104	12	0.9617406078194364
1104	13	0.7003640424095465
1104	14	1.0584113917206253
1104	15	1.047921078070985
1104	16	1.1311886574954784
1104	17	1.0522413540490732
1104	18	0.6087081733963586
1104	19	1.072208170928434
1104	20	1.0560778434036493
1104	21	0.9946367093316013
1104	22	0.9360820799129428
1104	23	0.9994350833958947
1104	24	1.204841724296159
1104	25	1.0291286608787829
1105	1	0.6402828478277591
1105	2	0.6797126510262146
1105	3	0.6672733819760914
1105	4	0.40625190949747036
1105	5	0.4850542742959689
1105	6	0.5994535768947316
1105	7	0.698592176619897
1105	8	0.6182936914257411
1105	9	0.9250527207380372
1105	10	0.5826604360188554
1105	11	0.9328563155405831
1105	12	1.1269836287113222
1105	13	0.9395734450152275
1105	14	1.1419800418717756
1105	15	1.0864392113081252
1105	16	1.1046581194407068
1105	17	0.7819058143584101
1105	18	0.7985136280485818
1105	19	0.9905595270412206
1105	20	0.93114768342177
1105	21	0.8062266568432293
1105	22	0.8674730358053679
1105	23	1.1068379854968313
1105	24	1.020554679724384
1105	25	0.9407709010165917
1106	1	0.2894688318804022
1106	2	0.5204408278230765
1106	3	-1.2480949467533975
1106	4	-0.13453636282575587
1106	5	0.11086843526148726
1106	6	0.40170309862482867
1106	7	0.5242165378148869
1106	8	0.5624759688141623
1106	9	0.8473843575522366
1106	10	0.620913442825433
1106	11	0.693294084766805
1106	12	0.8627272412413518
1106	13	0.8045947162792402
1106	14	0.8983409445112187
1106	15	0.9077295017040896
1106	16	0.8828602654774755
1106	17	0.9055102465774993
1106	18	0.9464077903134651
1106	19	0.9302530862625744
1106	20	0.8849431567668518
1106	21	0.749356218389515
1106	22	0.8035177060398695
1106	23	0.8114965489353168
1106	24	0.9103159334237958
1106	25	0.8657016113387499
1107	1	0.6175227102638416
1107	2	0.7526320405630571
1107	3	0.660879868352481
1107	4	0.47195647559675535
1107	5	0.7704300865029339
1107	6	0.708688767720588
1107	7	0.6118580274886511
1107	8	0.6337807473093093
1107	9	0.6161791252462621
1107	10	0.7879849327886461
1107	11	0.8286294719601344
1107	12	1.0310676738921174
1107	13	0.8674896013290976
1107	14	0.9672483500994886
1107	15	0.7866929960406281
1107	16	0.9751580397736603
1107	17	0.9267603357510694
1107	18	0.9724966137944762
1107	19	0.9000726491081181
1107	20	0.9998425837996265
1107	21	0.9346682324220765
1107	22	0.9535638255823642
1107	23	1.0882179064430204
1107	24	1.0701700055845293
1107	25	1.0580425536178666
1108	1	0.4798983399184067
1108	2	0.49334911236736545
1108	3	0.441977232511622
1108	4	0.30998086019167204
1108	5	0.4093158503462888
1108	6	0.43234045614930494
1108	7	0.48652253926347555
1108	8	0.40505549482758596
1108	9	0.5601132458456419
1108	10	0.5010980653680903
1108	11	0.5671765289919648
1108	12	0.6010844603493908
1108	13	0.6817775477042574
1108	14	0.7271032608826573
1108	15	0.7319065385526642
1108	16	0.6198583734130239
1108	17	0.6110786792245264
1108	18	0.6173764736297755
1108	19	0.7094594653669546
1108	20	0.6466590093854236
1108	21	0.7578365536063529
1108	22	0.788927909147155
1108	23	0.6826161477359776
1108	24	0.6965106997445432
1108	25	0.6978052671963866
1109	1	0.40592750030623037
1109	2	0.2893278366493668
1109	3	0.46045237934382044
1109	4	0.3643003196474055
1109	5	0.20022656177322062
1109	6	0.47763326317996047
1109	7	0.3957407221739402
1109	8	0.48267234040595497
1109	9	0.616206411316135
1109	10	0.3120258627631358
1109	11	0.6108947875713611
1109	12	0.7103946059062167
1109	13	0.5527945742533831
1109	14	0.7768475677393809
1109	15	0.6974927681421548
1109	16	0.7069696728362445
1109	17	0.7942712471895064
1109	18	0.912021225231598
1109	19	0.8365699200119394
1109	20	0.9570620484860726
1109	21	0.9485903244961449
1109	22	0.9512133100332747
1109	23	1.0068429102886185
1109	24	0.929135050389668
1109	25	0.997500961004981
1110	1	0.43047044118511124
1110	2	0.3249788842218919
1110	3	0.38539459276600496
1110	4	0.44362395501192253
1110	5	0.5388127313735538
1110	6	0.5731537130866258
1110	7	0.49918087708558545
1110	8	0.5212597093503533
1110	9	0.5129486657340863
1110	10	0.6916062667894423
1110	11	0.6496908423835706
1110	12	0.7197885228330989
1110	13	0.7350274912326071
1110	14	0.8089326383044266
1110	15	0.7798256668041219
1110	16	0.7422004048286368
1110	17	0.8423393219587965
1110	18	0.7320865785801729
1110	19	0.8263340635494316
1110	20	0.9260080867255169
1110	21	0.861220725905703
1110	22	0.9689233696951902
1110	23	0.9863411047306414
1110	24	1.004950822418344
1110	25	0.9209137926781281
1111	1	0.2878155444496002
1111	2	0.06257048981816382
1111	3	0.02914042887526591
1111	4	0.2554285852584215
1111	5	0.2663776880690918
1111	6	0.19598432601134874
1111	7	0.060806460030285336
1111	8	0.203400781968611
1111	9	0.20136055780604067
1111	10	0.13376982441769059
1111	11	0.10049447329629402
1111	12	0.32211197386574497
1111	13	0.2590364852366065
1111	14	-0.0051829513025012375
1111	15	0.0016902570772785452
1111	16	0.005545674648757881
1111	17	0.24850604414400834
1111	18	-0.031899930413835875
1111	19	-0.16350604304616922
1111	20	-0.06716729635243013
1111	21	0.22065610232142063
1111	22	-0.034645084668568416
1111	23	0.03236042518366844
1111	24	0.18322364053960172
1111	25	-0.03581921864765292
1112	1	0.06869879318961836
1112	2	-0.3301063690719843
1112	3	8.326608277258174E-4
1112	4	0.13576048358234516
1112	5	0.004956252827795993
1112	6	0.17984671315708312
1112	7	0.24498866965938504
1112	8	0.26638891036493817
1112	9	0.21304598483504833
1112	10	-0.10824665366430253
1112	11	0.08103243683153494
1112	12	0.4864965831445218
1112	13	0.2310633892104832
1112	14	0.1599798094451259
1112	15	0.009603990625727477
1112	16	-0.183315254299103
1112	17	0.03199487399148637
1112	18	0.16059579086791587
1112	19	0.16776998266448828
1112	20	0.18648288063801033
1112	21	0.15458100914391792
1112	22	0.1981820711677429
1112	23	0.42999114348213396
1112	24	0.2694394787982546
1112	25	0.28271318322276784
1113	1	-0.8835036890557468
1113	2	-0.7815108570805158
1113	3	-0.8063159335497829
1113	4	-2.317006062829293
1113	5	-1.0289883390992358
1113	6	-0.7320882583674333
1113	7	-0.8426924503006816
1113	8	-0.7515424761982601
1113	9	-0.7895925920714433
1113	10	-0.8699481489108294
1113	11	-0.9726274002569326
1113	12	-0.7581441265870623
1113	13	-0.932013097527431
1113	14	-1.0870151761469558
1113	15	-1.0986098818131822
1113	16	-1.2753854560099
1113	17	-1.3756256646602907
1113	18	-1.121168327753459
1113	19	-1.0015606732926727
1113	20	-0.7394881220166738
1113	21	-0.8751909600889434
1113	22	-0.9506317150505765
1113	23	-0.756446584832247
1113	24	-0.6986196503738071
1113	25	-1.0780882144583543
1114	1	-1.1396835209781848
1114	2	-2.3462336274708306
1114	3	-1.8115244935788013
1114	4	-1.261417637680367
1114	5	-1.2839403564602847
1114	6	-0.904971704510686
1114	7	-0.8485559841098436
1114	8	-0.848050348301939
1114	9	-1.1461073495762264
1114	10	-1.2949721555995026
1114	11	-1.369824673146283
1114	12	-1.4139521468498089
1114	13	-1.3302682631151796
1114	14	-1.4083890595746595
1114	15	-1.55151644323157
1114	16	-1.8223607361903973
1114	17	-2.0450627216431343
1114	18	-1.7805030258716528
1114	19	-2.1990797157301003
1114	20	-1.7888329638061435
1114	21	-1.7868036808674859
1114	22	-1.5563962712188513
1114	23	-1.3912484758584824
1114	24	-1.422062473880068
1114	25	-2.0325332941630236
1115	1	-1.1431180414337057
1115	2	-1.3540041475372462
1115	3	-0.876544178913548
1115	4	-0.7295041889928839
1115	5	-0.9227703454340102
1115	6	-0.8589728542232322
1115	7	-0.7423162323070116
1115	8	-0.9712148570585342
1115	9	-2.1004180472323526
1115	10	-1.8182058437293662
1115	11	-1.8897288775653749
1115	12	-2.726291325503862
1115	13	-2.5585596774737525
1115	14	-2.124546789580549
1115	15	-2.480377332670308
1115	16	-2.193523467083672
1115	17	-2.214536663475542
1115	18	-2.3865695705301206
1115	19	-3.0583795035351784
1115	20	-2.953515056903882
1115	21	-2.400436493104735
1115	22	-2.329017072530206
1115	23	-2.304508433522983
1115	24	-2.845832832510702
1115	25	-2.776816423554319
1116	1	-2.0351636056090605
1116	2	-2.081628538301956
1116	3	-2.183520622458712
1116	4	-1.808513757958515
1116	5	-2.44918855000844
1116	6	-3.3167634123464325
1116	7	-2.7665872402320595
1116	8	-3.169603028535839
1116	9	-4.2367372177076135
1116	10	-3.1133596816705307
1116	11	-3.957911134266805
1116	12	-6.071419179696441
1116	13	-5.1123260990099055
1116	14	-4.47436490334049
1116	15	-5.3949782698861135
1116	16	-5.0790898163284055
1116	17	-5.092513378435111
1116	18	-5.082430114979701
1116	19	-5.293463288261112
1116	20	-7.174571797950101
1116	21	-5.9629386637768
1116	22	-4.9636341145248295
1116	23	-5.719329010593739
1116	24	-5.625918025761617
1116	25	-4.8665722663603574
1201	1	0.6706777721433497
1201	2	0.8560280266173095
1201	3	0.9171034663106497
1201	4	0.7194579157861511
1201	5	0.36927605821823306
1201	6	0.9248296689392745
1201	7	1.090009833636067
1201	8	1.0492376815837972
1201	9	0.9823042227179641
1201	10	1.1153331739037
1201	11	0.8085806851866693
1201	12	1.3476987773598594
1201	13	1.2520239469567045
1201	14	1.3851286018700009
1201	15	1.1852185213235344
1201	16	1.1487258666863482
1201	17	1.0332929249998983
1201	18	1.346579315569187
1201	19	1.2834079240555525
1201	20	1.4230957662348303
1201	21	1.1256305947858536
1201	22	1.2650634604139626
1201	23	1.226269301006397
1201	24	0.9812835987065895
1201	25	1.1647932597512565
1202	1	1.0430613330531067
1202	2	1.027013689266731
1202	3	1.0187920541087685
1202	4	0.9676989103553737
1202	5	0.9763337064200144
1202	6	1.1161657523122201
1202	7	1.2360500469248166
1202	8	1.040384102652773
1202	9	1.5057795978380852
1202	10	1.5298875308855742
1202	11	1.4777174513726123
1202	12	1.5204175486887905
1202	13	1.5860209867128248
1202	14	1.7250692151768448
1202	15	1.5618530967519817
1202	16	1.7029133534239875
1202	17	1.6018654888931039
1202	18	1.7250681701576767
1202	19	1.7634856071324236
1202	20	1.6069615040793224
1202	21	1.7303100480282865
1202	22	1.7151330885844587
1202	23	1.84641228456573
1202	24	1.9002787999727886
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2211	9	-0.1594940776801579
2211	10	-0.14849399464372226
2211	11	-0.07428782085784036
2211	12	-0.10765038532034765
2211	13	-0.1095416433146994
2211	14	-0.32698968518280225
2211	15	-0.3121725948468992
2211	16	-0.3282293940130714
2211	17	-0.39610894059998003
2211	18	-0.23565853770312373
2211	19	-0.19677281541063962
2211	20	-0.26126395354244636
2211	21	-0.2176855666452724
2211	22	-0.30346150439932984
2211	23	-0.2307741437216393
2211	24	-0.09751032570896001
2211	25	-0.3497499797660879
2212	1	-0.10592173742593047
2212	2	-0.12047086353294745
2212	3	-0.018470930150733977
2212	4	-0.04806022867980175
2212	5	-0.3603008326180017
2212	6	-0.1258849376701079
2212	7	-0.32260165538427543
2212	8	-0.20015094040455494
2212	9	-0.21956390179963847
2212	10	-0.4924007638735546
2212	11	-0.20423394025623764
2212	12	0.13693359063516652
2212	13	0.10163586720312168
2212	14	0.06975100389890088
2212	15	-0.08734601196792369
2212	16	-0.011376422607050074
2212	17	-0.1183513557040925
2212	18	-0.07684911679354656
2212	19	0.07105659387251961
2212	20	-0.01665734853155243
2212	21	-0.03111486749656933
2212	22	0.02710232958641967
2212	23	-0.30042496918971295
2212	24	-0.27927088497726515
2212	25	-0.18745345659733914
2213	1	-0.36933454402066346
2213	2	-0.09353407193340146
2213	3	-0.0028406354237198173
2213	4	-0.31225394779449545
2213	5	-0.09776262951589132
2213	6	-0.14048891669907704
2213	7	-0.5244809179471446
2213	8	-0.4472448409948413
2213	9	-0.39588023680335027
2213	10	-0.2129989963684551
2213	11	-0.2298589424276969
2213	12	-0.39107514613488803
2213	13	-0.3252444087046271
2213	14	-0.4678034416740573
2213	15	-0.6569542354643261
2213	16	-1.0043156670802702
2213	17	-0.6437468207830648
2213	18	-0.31917485332156303
2213	19	-0.18207174654650515
2213	20	-0.2553943386308859
2213	21	-0.32241932609824175
2213	22	-0.4750182827749967
2213	23	-0.4943838123320319
2213	24	-0.294409215239682
2213	25	-0.3537819169963967
2214	1	-1.3517664009201518
2214	2	-0.5803970556277314
2214	3	-0.740244504978353
2214	4	-0.7650508570181529
2214	5	-1.1391759904309566
2214	6	-1.6372880162366812
2214	7	-1.1021537093805804
2214	8	-0.9775654786195673
2214	9	-0.8119291538147877
2214	10	-1.1751418535113132
2214	11	-1.277179458368309
2214	12	-0.8611889207759233
2214	13	-1.2249913057598718
2214	14	-0.9002778880113667
2214	15	-1.0958980174063981
2214	16	-0.9914932041939224
2214	17	-1.0543242118757874
2214	18	-1.1231295868333646
2214	19	-1.2201321018641025
2214	20	-1.39479078545493
2214	21	-1.549531153793545
2214	22	-1.9858980588762378
2214	23	-1.8698243088031958
2214	24	-1.5073424106643643
2214	25	-1.9722347035624708
2215	1	-1.715511066638502
2215	2	-1.188001201126614
2215	3	-1.2795591767930254
2215	4	-1.341146670877123
2215	5	-1.7169127309308543
2215	6	-1.532669544381367
2215	7	-1.9535218256171198
2215	8	-1.7864483276339982
2215	9	-1.7983333399558177
2215	10	-1.6632123676344577
2215	11	-1.3642227422537019
2215	12	-1.624432672045991
2215	13	-1.8078423522951326
2215	14	-2.013915868096717
2215	15	-1.6898667229540507
2215	16	-1.8405254130945465
2215	17	-2.00194410620621
2215	18	-1.6612011170101582
2215	19	-1.832627529622684
2215	20	-1.9299929801308124
2215	21	-1.5473319056732464
2215	22	-1.8412739987258675
2215	23	-2.2516034187401957
2215	24	-2.1063400737409737
2215	25	-2.280250858815471
2216	1	-2.627663379518559
2216	2	-1.78822152658271
2216	3	-1.8259718294792826
2216	4	-2.235422387501938
2216	5	-3.016661902546538
2216	6	-3.3265466038513303
2216	7	-3.1879119073303457
2216	8	-3.554105404283497
2216	9	-5.553239327065793
2216	10	-3.414845408454063
2216	11	-3.584729365448222
2216	12	-3.9006813073234916
2216	13	-3.8134488322870617
2216	14	-4.13963479107473
2216	15	-4.06134493305894
2216	16	-3.3220092104285577
2216	17	-3.6281499717223227
2216	18	-4.064281196449095
2216	19	-4.297674815130691
2216	20	-3.614848335961497
2216	21	-4.196154707537328
2216	22	-3.7664181116308733
2216	23	-4.78429796146191
2216	24	-4.190722753569464
2216	25	-4.1708647317382415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56345.0
3	1.0
4	905.0
5	7944.0
6	10510.0
7	8165.0
8	6678.0
9	5520.0
10	4610.0
11	4106.0
12	3555.0
13	3177.0
14	3075.0
15	3129.0
16	3173.0
17	3392.0
18	3580.0
19	4559.0
20	6250.0
21	8259.0
22	10276.0
23	12068.0
24	13931.0
25	15866.0
26	17806.0
27	19218.0
28	21518.0
29	25065.0
30	31445.0
31	40264.0
32	51072.0
33	65889.0
34	85655.0
35	112826.0
36	155098.0
37	243488.0
38	783815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9486999650399	20.19229678156311	14.084004752845033	25.77499850055196
2	12.607725329306257	21.257618279116897	42.216392613861004	23.918263777715847
3	17.063539205225347	26.651820620596407	30.3243270680427	25.96031310613555
4	10.887693512952875	17.84673595959694	38.408821809617756	32.85674871783243
5	11.65429834835297	40.05472191128182	35.58020399490472	12.710775745460495
6	30.152067633633145	38.44264376198579	18.47473192339897	12.930556680982091
7	26.872983352459745	32.786717843319046	22.407993326287897	17.932305477933312
8	24.571068964958435	38.31268940110089	20.717198835368915	16.399042798571763
9	26.738549801760662	14.824155066254383	20.632767434099325	37.804527697885625
10	15.091288277149491	30.16167269745637	34.03083075816183	20.716208267232314
11	32.99203185960351	23.175907038721988	25.575689774885724	18.25637132678878
12	23.31077487886191	25.909433249200287	32.14571663809742	18.63407523384038
13	28.729446725891915	23.120577408378857	26.472325496264315	21.67765036946491
14	20.18246192950953	23.909930883015104	28.43903160636252	27.468575581112848
15	22.060217882428155	33.04154193718386	24.030565742696574	20.867674437691413
16	20.3556971860302	28.89965395681938	29.661607672666584	21.083041184483832
17	19.938504066884253	30.000134977904118	29.04783886877718	21.01352208643445
18	19.804097094067302	27.656140835853837	32.5965287758714	19.943233294207463
19	22.428145063934643	27.128437256371328	29.3828865502936	21.060531129400434
20	22.93223928833085	27.56870309992015	30.071120483703513	19.427937128045485
21	21.837345982617673	26.916693751194508	29.0109306315752	22.235029634612623
22	22.195311572059477	28.978739026681495	29.087689367353768	19.73826003390526
23	20.68692221766916	28.5209258230471	30.10744328602287	20.684708673260868
24	22.137333693979105	27.14231956778656	30.634266855195865	20.086079883038472
25	21.299869562776152	27.958484682264423	30.36174734050594	20.379898414453482
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	518.0
3	876.0
4	876.0
5	876.0
6	2321.0
7	3766.0
8	3766.0
9	3766.0
10	4636.5
11	5507.0
12	5507.0
13	5507.0
14	7614.0
15	9721.0
16	9721.0
17	9721.0
18	18033.0
19	26345.0
20	26345.0
21	26345.0
22	42663.5
23	58982.0
24	58982.0
25	58982.0
26	87237.0
27	115492.0
28	115492.0
29	115492.0
30	145303.5
31	175115.0
32	175115.0
33	175115.0
34	189870.0
35	204625.0
36	204625.0
37	204625.0
38	210856.5
39	217088.0
40	217088.0
41	217088.0
42	223937.5
43	230787.0
44	230787.0
45	230787.0
46	235586.5
47	240386.0
48	240386.0
49	240386.0
50	222478.0
51	204570.0
52	204570.0
53	204570.0
54	174891.0
55	145212.0
56	145212.0
57	145212.0
58	121630.5
59	98049.0
60	98049.0
61	98049.0
62	79584.5
63	61120.0
64	61120.0
65	61120.0
66	46056.0
67	30992.0
68	30992.0
69	30992.0
70	22804.5
71	14617.0
72	14617.0
73	14617.0
74	10282.5
75	5948.0
76	5948.0
77	5948.0
78	4001.0
79	2054.0
80	2054.0
81	2054.0
82	1342.5
83	631.0
84	631.0
85	631.0
86	392.5
87	154.0
88	154.0
89	154.0
90	93.5
91	33.0
92	33.0
93	33.0
94	18.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08379075418697324
2	0.041301499325408844
3	0.01819425525838272
4	0.005182933248678757
5	0.017492399714290806
6	0.058469965711657225
7	0.07499056544182076
8	0.05415085467109159
9	0.023269210731047336
10	0.048589999206363345
11	0.005776811016756531
12	4.319111040565631E-4
13	0.005128944360671687
14	5.938777680777743E-4
15	0.012147499801590836
16	0.008206310977074699
17	0.00421113326455149
18	0.004912988808643405
19	0.0015656777522050412
20	0.0012957333121696893
21	3.779222160494927E-4
22	7.01855544091915E-4
23	0.0
24	0.0
25	5.398888800707039E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1852233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.36050419826961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3559465539652	44.3717609706984
2	13.501910046849478	15.489527358539359
3	4.316175734566832	7.427340490292705
4	1.851697466317825	4.248572011625952
5	0.9073396028918247	2.60227285504664
6	0.5421884692065625	1.866012237850584
7	0.32981503140306057	1.3242849545413378
8	0.2139347313069937	0.9817123242632395
9	0.15157408890796584	0.7824929546838839
>10	0.7285569706175449	7.327469075550202
>50	0.053344294428087004	2.1234162171348525
>100	0.039488400706049934	4.46106333636621
>500	0.004911619521175817	1.9772236704190131
>1k	0.002739172425271129	3.109465142014797
>5k	2.833626646832202E-4	1.2130229834222417
>10k+	9.44542215610734E-5	0.6943634175505583
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12816	0.691921588698614	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9647	0.520830802604208	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7555	0.40788604889341673	No Hit
GTCCTACAGTGGACATTTCTAAATT	5187	0.2800403620926741	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4613	0.24905074037661568	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4354	0.23506761838278448	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4299	0.2320982295423956	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4291	0.23166631843833901	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3368	0.18183457480781307	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2831	0.15284254194801625	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2732	0.1474976420353163	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2374	0.12816962012878508	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2097	0.1132146981508266	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1945	0.1050083871737519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6196666402121116E-4	0.0
5	0.0	0.0	0.0	1.6196666402121116E-4	0.0
6	0.0	0.0	0.0	1.6196666402121116E-4	0.0
7	0.0	0.0	0.0	1.6196666402121116E-4	0.0
8	0.0	0.0	0.0	1.6196666402121116E-4	0.0
9	0.0	0.0	0.0	2.1595555202828153E-4	0.0
10	0.0	0.0	0.0	2.1595555202828153E-4	0.0
11	0.0	0.0	0.0	2.6994444003535195E-4	0.0
12	0.0	0.0	0.0	2.6994444003535195E-4	0.0
13	0.0	0.0	0.0	2.6994444003535195E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	35	9.8616954E-5	19.019825	5
GGTATCA	1960	0.0	13.68485	1
GGCGTGC	135	2.7284841E-11	12.667207	8
AGCACCG	100	1.4235229E-7	12.362885	5
GGCGAGG	1300	0.0	11.901756	19
GTCTAAG	160	3.6379788E-12	11.889319	1
TAGGACC	1010	0.0	11.863851	4
CACCTTT	660	0.0	11.506626	14
AGGACCG	75	2.0542281E-4	11.411895	5
TCCAACG	225	0.0	11.390945	18
TGGCGAG	2830	0.0	11.270194	18
CGTGCGC	110	4.9859227E-7	11.224416	10
GATATAC	715	0.0	11.174297	1
TTAGGAC	1815	0.0	11.1095	3
GCGTGCA	60	0.005886335	11.080812	9
TGTCGAG	120	1.2896453E-7	11.074531	18
GAACAGT	525	0.0	11.049314	6
GCGTGCG	130	3.277637E-8	10.959045	9
CGCCCTA	295	0.0	10.940751	16
CCACCTT	660	0.0	10.931295	13
>>END_MODULE