FastQCFastQC Report
Thu 26 May 2016
SRR1512865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429559
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45060.3152021007877254No Hit
GTCCTACAGTGGACATTTCTAAATT27400.19166750025707227No Hit
TATCAACGCAGAGTACTTTTTTTTT26420.1848122392989726No Hit
GTACTGGTTCACTATCGGTCAGTCA26360.1843925294443951No Hit
GGTATCAACGCAGAGTACTTTTTTT26120.18271369002608495No Hit
GTCCTAAAGTGTGTATTTCTCATTT25480.1782367849105913No Hit
CTGTAGGACGTGGAATATGGCAAGA23120.16172819729720844No Hit
CTTTAGGACGTGAAATATGGCGAGG20070.14039294635618396No Hit
GGATACCACGTGTCCCGCCCTACTC16760.1172389527119902No Hit
GTCCTACAGTGTGCATTTCTCATTT15250.10667625470512235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT759.684809E-713.9315874
CTCCTAA1009.7061275E-913.3347571
TAGGACC4000.013.2983344
GTCCTAC13250.011.8611041
GGTATCA15350.011.5414831
GGCGAGG6550.011.45577719
TCCTACA14800.011.1675192
CGCCCTA3050.010.8995116
GTTCAAT1408.010829E-910.8855161
TAGACTC700.001492833410.85578255
GTCTAGA1605.2023097E-1010.7154291
GCACCGT803.7734178E-410.6865356
GTATAGG1252.1597225E-710.6678051
GTGTAGC3050.010.6178391
TCCCGCC3150.010.55386413
TAGGACA5100.010.4300654
CTAGAGT1553.6670826E-910.4180494
CTACAGT17050.010.4180484
AGGACCT8150.010.3729325
CCTACAG14850.010.3623373
Produced by FastQC (version 0.11.5)