FastQCFastQC Report
Thu 26 May 2016
SRR1512865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429559
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62880.439855927597252No Hit
TATCAACGCAGAGTACTTTTTTTTT45180.3160415204968805No Hit
GGTATCAACGCAGAGTACTTTTTTT36820.25756194742574456No Hit
GTCCTACAGTGGACATTTCTAAATT26700.1867708852870011No Hit
GTACTGGTTCACTATCGGTCAGTCA23630.16529573106111745No Hit
CTGTAGGACGTGGAATATGGCAAGA22510.15746114710900355No Hit
GTCCTAAAGTGTGTATTTCTCATTT22370.15648182411498932No Hit
CTTTAGGACGTGAAATATGGCGAGG22140.15487293633910879No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21990.15382366170266495No Hit
GTCCTACAGTGTGCATTTCTCATTT15450.10807528755371412No Hit
GGATACCACGTGTCCCGCCCTACTC15080.10548707678381936No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14410.10080031674103691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT400.00529802114.24220710
TAGGACC4500.012.4701234
GGTATCA14550.012.36027051
ATTACCC550.003036703412.1075963
CCCCGTA1850.011.28874915
TCCAACG1604.5474735E-1111.2715318
TAGCACC855.2589312E-511.1895434
GCTATTC600.00581525411.0986293
ACCGTGC600.00587776411.08278
GGACCGT951.3439299E-511.0117066
TGTCGAA2000.010.91673314
GCCTACG700.001471196510.8747971
GCCTTAC1053.398165E-610.8747961
GGCGAGG6650.010.8470319
GTCGAAA1855.456968E-1210.77562315
TAGACTG1158.681036E-710.7516915
GTAGGAC12400.010.7406093
GCGCCAC909.5862066E-510.54756813
GTACTAG1002.3553835E-510.466991
TGGCGAG14450.010.44428118
Produced by FastQC (version 0.11.5)