##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429559 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.130007226004665 31.0 30.0 34.0 26.0 34.0 2 30.114977416112243 31.0 30.0 34.0 26.0 34.0 3 30.300589202684183 31.0 30.0 34.0 26.0 34.0 4 33.90322470076436 37.0 35.0 37.0 30.0 37.0 5 33.472482073142835 37.0 35.0 37.0 28.0 37.0 6 33.33449336473696 37.0 35.0 37.0 27.0 37.0 7 33.157723465768115 37.0 35.0 37.0 26.0 37.0 8 33.09484673245385 37.0 35.0 37.0 26.0 37.0 9 34.39456993380476 39.0 35.0 39.0 26.0 39.0 10 34.32232667556918 39.0 35.0 39.0 26.0 39.0 11 34.316436747276605 39.0 35.0 39.0 26.0 39.0 12 34.17327231684737 39.0 35.0 39.0 25.0 39.0 13 34.16359170905153 39.0 35.0 39.0 25.0 39.0 14 35.21034598781862 39.0 35.0 41.0 25.0 41.0 15 35.19893197832339 39.0 35.0 41.0 25.0 41.0 16 35.19110369001909 39.0 35.0 41.0 25.0 41.0 17 35.18119014325397 39.0 35.0 41.0 25.0 41.0 18 35.10589209679349 39.0 35.0 41.0 25.0 41.0 19 35.11603788301147 40.0 35.0 41.0 25.0 41.0 20 35.090602766307654 40.0 35.0 41.0 24.0 41.0 21 35.05236160242424 40.0 35.0 41.0 24.0 41.0 22 35.01470313572228 40.0 35.0 41.0 24.0 41.0 23 34.846692581418466 39.0 35.0 41.0 23.0 41.0 24 34.796713531935374 39.0 35.0 41.0 22.0 41.0 25 34.734853195985615 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6676081944293699 1101 2 0.5250300007623885 1101 3 0.5155534673672655 1101 4 0.5518998218480107 1101 5 0.7880119438581161 1101 6 0.6717795131021518 1101 7 0.704498648079884 1101 8 0.39459915932894774 1101 9 0.6881124042185007 1101 10 0.45331035758362503 1101 11 0.7282328998717134 1101 12 0.7705508067246996 1101 13 0.9336410763502485 1101 14 0.8792459599183857 1101 15 0.6121272188333293 1101 16 0.8258950998191139 1101 17 0.9138473660360305 1101 18 0.9126014639952942 1101 19 0.8676446954409514 1101 20 0.8292012960954196 1101 21 0.8640328978338943 1101 22 0.8199945013565753 1101 23 0.8715974834265481 1101 24 0.8444192032745761 1101 25 0.9838712431523007 1102 1 0.8767608155480247 1102 2 0.8997643613780681 1102 3 0.7092179142781063 1102 4 0.8321082896142329 1102 5 1.055537690444595 1102 6 0.9325322082126632 1102 7 0.8270459469467752 1102 8 0.8606663628667874 1102 9 1.1049132886793487 1102 10 0.9339842857771075 1102 11 1.0812407550541536 1102 12 1.1034425549114673 1102 13 1.202621924503461 1102 14 1.124373681915479 1102 15 1.1809359928914347 1102 16 1.1455190749027864 1102 17 1.2954743696237756 1102 18 0.7229717244294349 1102 19 0.964709111378454 1102 20 1.3979197312535945 1102 21 1.198605379744599 1102 22 0.9781075798481709 1102 23 1.2716189926884383 1102 24 1.1375452733603524 1102 25 1.1793074079648704 1103 1 0.5881207286741805 1103 2 0.7399371211065962 1103 3 0.4870611407812717 1103 4 0.3933229460053056 1103 5 0.5470336801881004 1103 6 0.47183039724090037 1103 7 0.5452931276875788 1103 8 0.7137240809630754 1103 9 0.7985644786596353 1103 10 0.6721958875945333 1103 11 0.8537793139354903 1103 12 0.9176345229414977 1103 13 1.0266656553215725 1103 14 0.9703010865490427 1103 15 0.8847818250287389 1103 16 0.8558302171746845 1103 17 0.8626250732010305 1103 18 0.9590217540992896 1103 19 1.0186937245629935 1103 20 0.7966498322639453 1103 21 1.030157587590935 1103 22 0.9175754651064594 1103 23 0.9691653504149826 1103 24 0.9428445860864727 1103 25 0.8685866784371257 1104 1 0.7814104913940945 1104 2 0.8791453958950086 1104 3 0.8286499262133162 1104 4 0.5588778776314385 1104 5 0.7284600570656607 1104 6 0.666971952259928 1104 7 0.7110853311534413 1104 8 0.873179621456373 1104 9 0.8459996752056469 1104 10 0.9356558593610416 1104 11 1.057464510481502 1104 12 1.1095222997517737 1104 13 0.8312860038864827 1104 14 1.1684789377690805 1104 15 1.2510491493774865 1104 16 1.2771204894827761 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-2.3689933322519003 2216 14 -2.4134401833155366 2216 15 -2.3031385551573393 2216 16 -1.6638279677402252 2216 17 -1.9944210454064546 2216 18 -2.34504410305685 2216 19 -2.4800485486247794 2216 20 -1.7744109984876602 2216 21 -2.528118654205059 2216 22 -1.9301241839751384 2216 23 -3.1727644561149404 2216 24 -2.3764308677337453 2216 25 -2.595558661493669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42272.0 3 0.0 4 695.0 5 5974.0 6 7439.0 7 5794.0 8 4635.0 9 3876.0 10 3256.0 11 2997.0 12 2746.0 13 2611.0 14 2506.0 15 2514.0 16 2448.0 17 2658.0 18 3015.0 19 3546.0 20 4553.0 21 6286.0 22 7823.0 23 9324.0 24 11166.0 25 12824.0 26 13929.0 27 15410.0 28 17609.0 29 20715.0 30 26170.0 31 33370.0 32 42112.0 33 53899.0 34 69882.0 35 91458.0 36 124382.0 37 190846.0 38 578819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.30836593582933 18.880695868421274 13.375331509273858 26.435606686475545 2 14.013179258064879 20.191026459292104 39.497813721208864 26.29798056143415 3 17.69784514936468 25.028213367924007 29.167549974217916 28.106391508493395 4 11.890354381109749 16.681683803458323 36.00803929826132 35.419922517170605 5 13.279095248290368 38.60139625287038 34.08477429484192 14.03473420399733 6 32.71932010347955 35.815377465198836 17.58263525696303 13.882667174358573 7 28.978871760406605 31.472466081404626 20.85787093431904 18.69079122386973 8 26.680338338891822 35.60712373446626 19.840979321012497 17.871558605629417 9 27.61293708128531 14.090713498574402 19.381273067571588 38.915076352568704 10 16.45952604246861 28.57903724751893 32.27201466944752 22.689422040564942 11 35.173402822714635 22.026897987023208 23.509750083072454 19.289949107189702 12 24.26412209148187 24.574988930090637 30.204099049281908 20.956789929145586 13 29.644662522438576 21.31647099809858 25.461538569163977 23.577327910298866 14 21.89242901082789 21.83241008178104 26.803628277169935 29.47153263022114 15 24.325664960044328 30.02195428080491 22.781939000961287 22.87044175818948 16 22.914699071644947 27.186041793467236 26.84310309848119 23.05615603640663 17 22.25247415663211 27.865226233090844 26.8927726714036 22.989526938873446 18 22.12753216426634 26.1729787283161 29.42875591395066 22.2707331934669 19 24.06717461987453 26.0090043706734 27.316428919195708 22.607392090256365 20 24.746367864705277 25.81392078940576 27.510771015109153 21.928940330779813 21 24.147758075426374 25.527699917386766 26.82964535067955 23.494896656507315 22 24.42200221468759 26.743436906936253 26.603042782084113 22.231518096292042 23 23.187220674347824 26.471660141344287 27.391664142578236 22.949455041729653 24 23.99516214440957 25.67658977348959 27.735966126616667 22.59228195548417 25 23.272072016614914 26.208641965809036 27.769053253485865 22.750232764090185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 73.0 2 263.5 3 454.0 4 454.0 5 454.0 6 1098.0 7 1742.0 8 1742.0 9 1742.0 10 1907.0 11 2072.0 12 2072.0 13 2072.0 14 2814.0 15 3556.0 16 3556.0 17 3556.0 18 7735.0 19 11914.0 20 11914.0 21 11914.0 22 19976.0 23 28038.0 24 28038.0 25 28038.0 26 43315.0 27 58592.0 28 58592.0 29 58592.0 30 75772.0 31 92952.0 32 92952.0 33 92952.0 34 105717.0 35 118482.0 36 118482.0 37 118482.0 38 128960.0 39 139438.0 40 139438.0 41 139438.0 42 151937.5 43 164437.0 44 164437.0 45 164437.0 46 181361.0 47 198285.0 48 198285.0 49 198285.0 50 193723.5 51 189162.0 52 189162.0 53 189162.0 54 169964.5 55 150767.0 56 150767.0 57 150767.0 58 132409.5 59 114052.0 60 114052.0 61 114052.0 62 96057.0 63 78062.0 64 78062.0 65 78062.0 66 60211.5 67 42361.0 68 42361.0 69 42361.0 70 31785.0 71 21209.0 72 21209.0 73 21209.0 74 15151.0 75 9093.0 76 9093.0 77 9093.0 78 6211.0 79 3329.0 80 3329.0 81 3329.0 82 2217.5 83 1106.0 84 1106.0 85 1106.0 86 706.5 87 307.0 88 307.0 89 307.0 90 184.5 91 62.0 92 62.0 93 62.0 94 37.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08694989153997841 2 0.045258712651943714 3 0.019026846740848053 4 0.005666083036796663 5 0.02133525094102447 6 0.06092788055617152 7 0.07967492072730122 8 0.05694063693768498 9 0.023363848571482534 10 0.05169426375546584 11 0.005875937964085428 12 5.596131394367074E-4 13 0.007065115885388431 14 6.995164242958842E-4 15 0.015739119546657396 16 0.010422794722008676 17 0.005736034679226251 18 0.006995164242958843 19 0.002168500915317241 20 0.0019586459880284756 21 4.1970985457753053E-4 22 8.394197091550611E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1429559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.299438269500236 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48350454453421 40.98711895381476 2 13.660799680281787 14.835474979029389 3 4.776018463615067 7.780053592171791 4 2.0831280414502373 4.524507299767683 5 1.1629360198672727 3.1573386311080642 6 0.6832412094161774 2.225976832432344 7 0.44606112411559407 1.6954607931337007 8 0.29842436104024755 1.296342013633598 9 0.21307066297557892 1.0412655580155543 >10 1.0700778039964305 10.141424975978518 >50 0.07116883735007593 2.688292796717279 >100 0.043556052193535484 4.678605376429551 >500 0.0056867865034312395 2.1481911049069624 >1k 0.0021971675126893427 2.358659093264231 >5k 1.2924514780525545E-4 0.4412879995966998 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6288 0.439855927597252 No Hit TATCAACGCAGAGTACTTTTTTTTT 4518 0.3160415204968805 No Hit GGTATCAACGCAGAGTACTTTTTTT 3682 0.25756194742574456 No Hit GTCCTACAGTGGACATTTCTAAATT 2670 0.1867708852870011 No Hit GTACTGGTTCACTATCGGTCAGTCA 2363 0.16529573106111745 No Hit CTGTAGGACGTGGAATATGGCAAGA 2251 0.15746114710900355 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2237 0.15648182411498932 No Hit CTTTAGGACGTGAAATATGGCGAGG 2214 0.15487293633910879 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2199 0.15382366170266495 No Hit GTCCTACAGTGTGCATTTCTCATTT 1545 0.10807528755371412 No Hit GGATACCACGTGTCCCGCCCTACTC 1508 0.10548707678381936 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1441 0.10080031674103691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 6.995164242958843E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCAT 40 0.005298021 14.242207 10 TAGGACC 450 0.0 12.470123 4 GGTATCA 1455 0.0 12.3602705 1 ATTACCC 55 0.0030367034 12.107596 3 CCCCGTA 185 0.0 11.288749 15 TCCAACG 160 4.5474735E-11 11.27153 18 TAGCACC 85 5.2589312E-5 11.189543 4 GCTATTC 60 0.005815254 11.098629 3 ACCGTGC 60 0.005877764 11.0827 8 GGACCGT 95 1.3439299E-5 11.011706 6 TGTCGAA 200 0.0 10.916733 14 GCCTACG 70 0.0014711965 10.874797 1 GCCTTAC 105 3.398165E-6 10.874796 1 GGCGAGG 665 0.0 10.84703 19 GTCGAAA 185 5.456968E-12 10.775623 15 TAGACTG 115 8.681036E-7 10.751691 5 GTAGGAC 1240 0.0 10.740609 3 GCGCCAC 90 9.5862066E-5 10.547568 13 GTACTAG 100 2.3553835E-5 10.46699 1 TGGCGAG 1445 0.0 10.444281 18 >>END_MODULE