FastQCFastQC Report
Thu 26 May 2016
SRR1512864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341381
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110870.47352395872350544No Hit
GTCCTACAGTGGACATTTCTAAATT91790.39203359043231323No Hit
CTGTAGGACGTGGAATATGGCAAGA71180.30400861713663857No Hit
GTCCTAAAGTGTGTATTTCTCATTT68550.2927759301027898No Hit
TATCAACGCAGAGTACTTTTTTTTT65800.2810307250293737No Hit
GGTATCAACGCAGAGTACTTTTTTT60650.25903515916461267No Hit
CTTTAGGACGTGAAATATGGCGAGG54450.23255506045363827No Hit
GTCCTACAGTGTGCATTTCTCATTT49230.21026052573246304No Hit
CTGTAGGACCTGGAATATGGCGAGA34140.14581138225688173No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33340.14239459532643342No Hit
CTGAAGGACCTGGAATATGGCGAGA30820.13163171649552122No Hit
GTCCTTCAGTGTGCATTTCTCATTT23760.10147857183431487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1205.2750693E-1113.45628910
TCCAACG1800.013.19300518
TAGGACC13650.013.0825414
GGTATCA22850.012.7931851
CCAACGT1051.9988875E-812.66501319
CGGTTTC1155.338734E-912.38968813
TTGCGAG853.9505376E-612.29277618
GACAGCG550.003071751712.089597
ACCGTGC550.003071751712.089598
GGCGTGC1501.4551915E-1112.032028
GCGTGCG1501.4551915E-1112.0315069
GCGTCGT658.033431E-411.6910326
ATACCGT658.033431E-411.6910326
AGGACCT23950.011.6203645
AGACCGT907.474031E-611.6098446
CCTACAC4000.011.3985133
GGACCTG23700.011.3025656
TGTACGC600.005882873711.0818872
ATTTCTA28700.011.0212115
GTATCAA49450.010.9950281
Produced by FastQC (version 0.11.5)