##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512864_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2341381
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.927674308453003	31.0	30.0	34.0	22.0	34.0
2	29.075143259469517	31.0	30.0	34.0	22.0	34.0
3	29.162691163890027	31.0	30.0	34.0	22.0	34.0
4	32.534207375903364	35.0	33.0	37.0	24.0	37.0
5	32.73820621248742	35.0	33.0	37.0	26.0	37.0
6	32.6028958977629	37.0	33.0	37.0	25.0	37.0
7	32.57062263681135	37.0	33.0	37.0	24.0	37.0
8	32.470485153847235	37.0	33.0	37.0	23.0	37.0
9	33.734901752427305	39.0	34.0	39.0	22.0	39.0
10	33.60316069875001	38.0	34.0	39.0	19.0	39.0
11	33.672109323514626	38.0	34.0	39.0	19.0	39.0
12	33.566077455997124	38.0	34.0	39.0	18.0	39.0
13	33.54237691345407	38.0	34.0	39.0	18.0	39.0
14	34.38733294581275	39.0	34.0	41.0	17.0	41.0
15	34.58341209739039	39.0	34.0	41.0	17.0	41.0
16	34.52937005980658	39.0	34.0	41.0	17.0	41.0
17	34.31737167082162	39.0	34.0	41.0	17.0	41.0
18	34.31319934688118	39.0	34.0	41.0	17.0	41.0
19	34.44163636759673	39.0	34.0	41.0	18.0	41.0
20	34.42591316833954	39.0	34.0	41.0	18.0	41.0
21	34.32779543354969	39.0	34.0	41.0	16.0	41.0
22	34.34283826510935	39.0	34.0	41.0	16.0	41.0
23	34.018464743670506	39.0	34.0	41.0	10.0	41.0
24	34.09205720897197	39.0	34.0	41.0	2.0	41.0
25	33.817784888491026	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8270126036453362
1101	2	0.5675364166703787
1101	3	0.8391565621913735
1101	4	0.9200392580461383
1101	5	0.845325382680123
1101	6	0.8365283861335158
1101	7	0.6447517627045585
1101	8	0.9672135501751669
1101	9	0.9946599433139269
1101	10	1.02292116134808
1101	11	0.9602888328494288
1101	12	0.891125704916071
1101	13	1.3579218258580639
1101	14	1.4218730672649116
1101	15	1.3011028654432195
1101	16	1.2115246686523307
1101	17	0.5536199376209723
1101	18	1.0634118543352642
1101	19	0.4623916663997534
1101	20	-3.1954632168407215E-4
1101	21	1.0815663921324372
1101	22	1.1008736160941837
1101	23	0.6050321564009664
1101	24	1.1459923798158442
1101	25	0.9187624571633464
1102	1	0.6963902661237675
1102	2	0.7637104177303904
1102	3	0.7228929783210596
1102	4	0.8151110079793895
1102	5	0.6727354113322761
1102	6	0.5554089198047762
1102	7	0.5603247459954517
1102	8	0.6195884111982721
1102	9	0.9789721920098344
1102	10	0.9429083296466061
1102	11	0.6849280906829449
1102	12	0.857982057604552
1102	13	0.8398984657853745
1102	14	1.0897607380719947
1102	15	0.9820940147572017
1102	16	0.9454386165524369
1102	17	1.0811383582855925
1102	18	1.1826032180422743
1102	19	0.9820452901599239
1102	20	1.1806508809899157
1102	21	1.137574776573402
1102	22	0.9234737491931995
1102	23	0.8312869334202659
1102	24	0.25675865555396626
1102	25	0.6427031444165294
1103	1	0.7685807669921196
1103	2	0.902457922951303
1103	3	1.0662586396727285
1103	4	1.1696865947730188
1103	5	0.995675194228653
1103	6	0.6052915456542323
1103	7	0.5719450953724916
1103	8	0.7427046409122866
1103	9	0.7741163570801533
1103	10	1.0628821402500677
1103	11	0.7482098781015409
1103	12	0.9347570909968042
1103	13	1.0590427912618878
1103	14	1.1872628742506848
1103	15	1.0250834927275676
1103	16	0.991707321611706
1103	17	0.7286725757038539
1103	18	1.0144928955949695
1103	19	0.9920999825039161
1103	20	1.1049174082265765
1103	21	0.824872919254716
1103	22	0.9398015577669057
1103	23	0.2250263103655783
1103	24	0.5917963047394394
1103	25	0.8087106033047249
1104	1	0.8981107495192262
1104	2	0.8363850571131728
1104	3	0.8600916904325686
1104	4	1.0300881968259716
1104	5	0.929749486201338
1104	6	0.7129064515052619
1104	7	0.9278153043182797
1104	8	0.6732116140575926
1104	9	1.1481528546571553
1104	10	1.1696823408116757
1104	11	0.754687566689995
1104	12	1.043315026798176
1104	13	1.0735120639485132
1104	14	1.3519009743076253
1104	15	1.1347491532312972
1104	16	1.0144907818667477
1104	17	1.1772631088665122
1104	18	1.1016614054704021
1104	19	0.7242527899547113
1104	20	0.8476156358975544
1104	21	0.9454708801506939
1104	22	0.9005526634329257
1104	23	1.2377696430492833
1104	24	1.0321322759337193
1104	25	0.6742794269541648
1105	1	0.604404659095465
1105	2	0.7045393541845364
1105	3	0.8305801475077921
1105	4	0.9742045310659577
1105	5	0.6456481219502166
1105	6	0.7819209016338036
1105	7	0.7637101715882224
1105	8	0.9004149711668319
1105	9	0.49700819424312215
1105	10	0.6832586175288853
1105	11	0.6538475438170437
1105	12	0.9171819131873491
1105	13	0.8670419681919483
1105	14	0.9664468812567719
1105	15	0.8880977638155514
1105	16	0.7359426523886157
1105	17	1.1260568303576974
1105	18	0.8842293587743981
1105	19	0.46564182688918976
1105	20	0.8395888661414475
1105	21	0.7476612821722384
1105	22	0.8208361548669032
1105	23	1.0371126745676662
1105	24	1.1285419261617875
1105	25	1.2799426735981712
1106	1	0.6737227329569855
1106	2	0.6904837015853111
1106	3	0.7112929434726887
1106	4	0.7216812608106196
1106	5	0.6608396362267257
1106	6	0.6974563873718509
1106	7	0.6492629772520857
1106	8	0.7991584733052974
1106	9	0.7802415675881846
1106	10	0.5432418223092128
1106	11	0.3255883731902358
1106	12	0.6542784759922498
1106	13	0.736136568087332
1106	14	0.9010227279926468
1106	15	0.617402324760171
1106	16	0.7643373451361839
1106	17	0.998008189404402
1106	18	0.8472692426825859
1106	19	0.7572057771267069
1106	20	1.0051791917620676
1106	21	0.8243962045561801
1106	22	0.699249027411156
1106	23	1.1446318985758168
1106	24	0.7463665407050257
1106	25	0.8952662658890134
1107	1	0.8521287406629057
1107	2	0.8057733384825525
1107	3	0.7346766470638251
1107	4	0.8531916403763162
1107	5	0.7596299268925577
1107	6	0.8684841495837077
1107	7	0.6929182559981228
1107	8	0.8139434578396347
1107	9	0.9299545742588435
1107	10	0.9680204432848996
1107	11	0.93033064906475
1107	12	0.939528950093198
1107	13	0.8803344905912169
1107	14	1.0999789144164254
1107	15	0.8933876474179243
1107	16	0.9657233978619075
1107	17	1.3009721514003445
1107	18	1.2913664879898548
1107	19	1.0820622804620328
1107	20	1.2033502226902257
1107	21	1.088752994409944
1107	22	1.0672605894590177
1107	23	1.2920780853478462
1107	24	1.1827167073922737
1107	25	1.3725489946842728
1108	1	0.6133128859824595
1108	2	0.6743390089863581
1108	3	0.6799505478714565
1108	4	0.7806863274648137
1108	5	0.6966623252478641
1108	6	0.6930068242275738
1108	7	0.6429822512131764
1108	8	0.6653475553354724
1108	9	0.7302682645151393
1108	10	0.9033850615468992
1108	11	0.5874048985315241
1108	12	0.7025426334415243
1108	13	0.6391493326755651
1108	14	0.6983466180930478
1108	15	0.7200466423819734
1108	16	0.6776870061880231
1108	17	0.8492476702163998
1108	18	0.9543294557202202
1108	19	0.8771383603249774
1108	20	0.9122741119345221
1108	21	0.8101423277398467
1108	22	0.7915982507825277
1108	23	1.07396853360612
1108	24	0.8357914168179548
1108	25	0.9823845636667414
1109	1	0.7046892477585054
1109	2	0.7166115850153112
1109	3	0.8556968693704796
1109	4	0.9065919362452632
1109	5	0.7749941756878442
1109	6	0.7806802571063116
1109	7	0.7471969397345433
1109	8	0.7005749889916046
1109	9	0.8030372202612099
1109	10	0.9165453633942491
1109	11	0.8008461968315572
1109	12	0.8027235937796178
1109	13	0.8239991988708084
1109	14	1.0441618672298958
1109	15	0.8466551727421177
1109	16	0.5704406724212276
1109	17	0.9557239325159088
1109	18	1.054228042172042
1109	19	1.0220734293968121
1109	20	0.8683161167719007
1109	21	0.9680149418203996
1109	22	1.0790725153728857
1109	23	1.3392577236364218
1109	24	0.9670403918136614
1109	25	0.6563440955287163
1110	1	0.576660625397043
1110	2	0.5741837637892253
1110	3	0.5582326292218056
1110	4	0.5073023561584904
1110	5	0.34614519592449255
1110	6	0.5008733774102012
1110	7	0.37908104029497736
1110	8	0.5820310303930611
1110	9	0.7786596042948304
1110	10	0.6501643723583257
1110	11	0.6708697473609959
1110	12	0.578396105560202
1110	13	0.6107613566949865
1110	14	0.615823392643783
1110	15	0.6069419017511777
1110	16	0.6533370737998183
1110	17	0.7767056243181472
1110	18	0.2248242926279289
1110	19	0.5794900716100528
1110	20	0.758373020495533
1110	21	0.9471548570078312
1110	22	0.8599194286975944
1110	23	0.9187415057983586
1110	24	0.7409955562279862
1110	25	1.100749570654152
1111	1	0.48968169206226975
1111	2	0.4925565082850696
1111	3	0.7150046741942511
1111	4	0.5032447426343722
1111	5	0.5505236942161673
1111	6	0.4412060828277333
1111	7	0.32819826983688927
1111	8	0.5744663541655228
1111	9	0.5323866174486795
1111	10	0.5567434546656784
1111	11	0.36770920782119276
1111	12	-0.008831115399587475
1111	13	0.42543218667734095
1111	14	0.6018666594612156
1111	15	0.5305664692719176
1111	16	0.5627174882242514
1111	17	0.17043312325212412
1111	18	0.44514177693453405
1111	19	0.5040713561206829
1111	20	0.7199828958659396
1111	21	0.7560422045943156
1111	22	0.3846199992193746
1111	23	0.6638351264256599
1111	24	0.6563888492235748
1111	25	0.7180978588189291
1112	1	0.3904864801269632
1112	2	0.4188293559852916
1112	3	0.4526130680010674
1112	4	0.5085256125868582
1112	5	0.5391367204949375
1112	6	0.27422266837583464
1112	7	0.20851032098430267
1112	8	0.4286918474548713
1112	9	0.4045126794602467
1112	10	0.47029750719588037
1112	11	0.34566310635331376
1112	12	0.4541774513133632
1112	13	0.425553209579995
1112	14	0.4459299308042475
1112	15	0.23786133118051112
1112	16	0.580118873067974
1112	17	0.7265957233594236
1112	18	0.5053035432652209
1112	19	0.5657938907560194
1112	20	0.2813694139715395
1112	21	0.4146981043042004
1112	22	0.4282896186550289
1112	23	0.5972896155495491
1112	24	0.7262996510729209
1112	25	0.6562557250797951
1113	1	-0.1895673276574925
1113	2	-0.07753763667014368
1113	3	-0.050100232078793994
1113	4	-0.037315739546471605
1113	5	-0.2682157008553645
1113	6	-0.6100873220670664
1113	7	-0.47690397277216334
1113	8	-0.4365694017759765
1113	9	-0.47822955375918497
1113	10	-0.44953085775443213
1113	11	-0.41493480155813245
1113	12	-0.6382318544222088
1113	13	-0.5179224329058982
1113	14	-0.1469277731351113
1113	15	-0.3790560457227201
1113	16	-0.6155496657430177
1113	17	-0.35452834875086126
1113	18	-0.5584933748971963
1113	19	-0.9272019329220242
1113	20	-0.6818717522565478
1113	21	-0.40611280911894454
1113	22	-0.8049854556416065
1113	23	-4.294397670888536
1113	24	-2.025981615701099
1113	25	-1.2422066291089635
1114	1	-0.8103099852138591
1114	2	-1.0083324551196746
1114	3	-0.8758848983373824
1114	4	-0.7900183440950492
1114	5	-0.6980833049533715
1114	6	-1.1801702906478688
1114	7	-1.1690820690547774
1114	8	-1.0702115353123247
1114	9	-1.2946700452234836
1114	10	-0.9714359012399285
1114	11	-0.9791021636732395
1114	12	-1.9681462637822982
1114	13	-1.2261443664002272
1114	14	-3.084643019323856
1114	15	-1.7868022111119615
1114	16	-1.7820055128293575
1114	17	-1.6034236933301145
1114	18	-1.6783532725525063
1114	19	-1.696696113608425
1114	20	-1.237304040895225
1114	21	-1.1888325655507472
1114	22	-1.3231105146002662
1114	23	-5.560362620287314
1114	24	-2.7168563677733566
1114	25	-2.6256258122742615
1115	1	-1.9144931188153222
1115	2	-1.7553891913410666
1115	3	-1.5370236953193555
1115	4	-1.4531009422399883
1115	5	-1.4401538733999928
1115	6	-1.532838566656185
1115	7	-2.048577994090863
1115	8	-2.317764990597368
1115	9	-3.6342206003198356
1115	10	-4.231549511041049
1115	11	-3.0518892787385568
1115	12	-3.36862036089223
1115	13	-2.6818313930260587
1115	14	-3.5089209241514006
1115	15	-2.9910287511519194
1115	16	-3.066425975124492
1115	17	-2.8684651839813142
1115	18	-5.4261267722741735
1115	19	-3.5098365167189876
1115	20	-3.8115677580527283
1115	21	-3.539392120027788
1115	22	-3.0246986299716276
1115	23	-3.616677428720635
1115	24	-3.278971854805043
1115	25	-3.6623176572858647
1116	1	-1.618854070811409
1116	2	-1.5188139891033678
1116	3	-1.8002230068713097
1116	4	-1.5291695216951204
1116	5	-1.7862103904079234
1116	6	-4.194130435276538
1116	7	-2.7156801646010393
1116	8	-3.0699456035303534
1116	9	-4.012698595786986
1116	10	-3.2760895800319183
1116	11	-2.7942232040365873
1116	12	-2.694716479486413
1116	13	-3.7336738983634774
1116	14	-6.865292294158142
1116	15	-4.918924286220655
1116	16	-3.7914747543950966
1116	17	-4.821444918826572
1116	18	-3.483130203559611
1116	19	-3.3664609120751265
1116	20	-3.9791857168578275
1116	21	-4.7542333120069316
1116	22	-4.404795044345292
1116	23	-3.4484266783171336
1116	24	-4.550439602669467
1116	25	-4.425589696508901
1201	1	0.5212415583661816
1201	2	0.6396993868673242
1201	3	0.9411881825802695
1201	4	0.8881682361414462
1201	5	0.4580212662043053
1201	6	0.9627162311669295
1201	7	1.0699971639927242
1201	8	1.0697432547145809
1201	9	1.385625896141562
1201	10	1.277879056716138
1201	11	1.4789941567203826
1201	12	1.260762886714339
1201	13	1.3134890482495791
1201	14	1.4233306058970285
1201	15	1.3730254825021717
1201	16	1.2160509930727699
1201	17	1.518650758872397
1201	18	1.352352060985119
1201	19	1.4213179585120344
1201	20	1.5043229161476148
1201	21	1.6045080012501955
1201	22	1.5045192983654445
1201	23	1.7463076679162555
1201	24	1.5278146335303617
1201	25	1.613950621606996
1202	1	1.07489084266426
1202	2	0.9661024606197373
1202	3	1.0111086300209067
1202	4	1.1663391670069885
1202	5	0.9012491913857517
1202	6	1.163355343743632
1202	7	1.2164372755484791
1202	8	1.2490759987344688
1202	9	1.5324691095122702
1202	10	1.2275826520275999
1202	11	1.303309781398589
1202	12	1.3653263084232847
1202	13	1.4062902205980734
1202	14	1.8302546346416335
1202	15	1.5614523937220284
1202	16	1.2505648320693865
1202	17	1.6257873732880341
1202	18	1.6661816875653912
1202	19	1.6667601136394055
1202	20	1.4872088064663416
1202	21	1.5686745091732277
1202	22	1.5075947630681128
1202	23	1.6083272246564633
1202	24	1.699767879427462
1202	25	1.9826244499303272
1203	1	0.8845747311879926
1203	2	1.0037428231735177
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2211	17	0.3680237898140035
2211	18	-0.042593240050443626
2211	19	0.13949484515686095
2211	20	-0.02655446961512098
2211	21	0.1276473207528852
2211	22	0.20548415146991772
2211	23	0.39754294416979263
2211	24	0.02622895471377973
2211	25	0.45267966758008527
2212	1	-0.06104070357206837
2212	2	0.03924499364035583
2212	3	-0.20926121584244228
2212	4	0.03289194308996457
2212	5	-0.234794827399476
2212	6	-0.37089045766059314
2212	7	-0.1976823953928175
2212	8	-0.18984495142347413
2212	9	-0.0907752421379655
2212	10	-0.39122675849906585
2212	11	-0.510160802775772
2212	12	0.07665248814426917
2212	13	0.11027254740658066
2212	14	0.04991926941371361
2212	15	-0.2745682274891621
2212	16	-0.18899035280689702
2212	17	-0.05252076194024369
2212	18	0.15178008891774653
2212	19	-0.23390505662738548
2212	20	-0.03791112009437825
2212	21	0.07440601921235412
2212	22	0.16053007642581463
2212	23	0.18418742135281718
2212	24	0.12276303012164647
2212	25	0.2862730907626414
2213	1	-0.38232788392597783
2213	2	-0.3116278530476464
2213	3	-0.5223081739002247
2213	4	-0.28852630329308937
2213	5	-0.25595984665411464
2213	6	-0.049222291694505316
2213	7	-0.38258704903648066
2213	8	-0.13583746037838296
2213	9	-0.5925481955033973
2213	10	-0.29571785992985156
2213	11	-0.46872743618524737
2213	12	-0.3587415186561955
2213	13	-0.4605522853533799
2213	14	-0.5714003685009317
2213	15	-0.4965309830742797
2213	16	-0.3832714251058391
2213	17	-0.3620337349692093
2213	18	-0.3999865946501089
2213	19	-0.37737292709518755
2213	20	-0.14900938872158775
2213	21	-0.4638498866643559
2213	22	-0.6547020060374678
2213	23	-0.4216458529172087
2213	24	-0.26604779827308533
2213	25	-0.05391267697861224
2214	1	-2.6310188938316337
2214	2	-1.4629889602877384
2214	3	-1.6309447780376445
2214	4	-3.3260660567789877
2214	5	-1.9057391985207595
2214	6	-1.564850750828434
2214	7	-1.4297204360731328
2214	8	-1.1336825296998505
2214	9	-1.1067676789318384
2214	10	-1.0049644612688127
2214	11	-1.1770202937570247
2214	12	-1.0434957806490814
2214	13	-1.434042785870588
2214	14	-1.3884932061322317
2214	15	-1.790042285130525
2214	16	-1.5224910501569653
2214	17	-3.565146261551785
2214	18	-3.2861419014784765
2214	19	-2.0778708724333654
2214	20	-2.0974301825563515
2214	21	-2.060366195365539
2214	22	-2.9442057261819947
2214	23	-1.906432518178974
2214	24	-2.854877960957886
2214	25	-2.8545466933864496
2215	1	-1.688977894910554
2215	2	-3.331110475886952
2215	3	-1.9091284348587045
2215	4	-3.267941646519109
2215	5	-1.897357464205495
2215	6	-1.7663856728417642
2215	7	-2.0516732418263643
2215	8	-1.5819124634985862
2215	9	-3.909421744426357
2215	10	-2.321249371064418
2215	11	-1.9180263575072232
2215	12	-1.872704410497171
2215	13	-1.9517712667000389
2215	14	-1.7008135586041604
2215	15	-1.7743460816471597
2215	16	-1.856807064397195
2215	17	-2.1230857142729533
2215	18	-1.7549088888133966
2215	19	-1.811435402690087
2215	20	-1.7390001412416538
2215	21	-2.2754060251444557
2215	22	-2.1562723259331307
2215	23	-2.3006856769304385
2215	24	-2.1361754456105473
2215	25	-3.0884623422227584
2216	1	-3.0763746015273306
2216	2	-3.0710964128376155
2216	3	-4.355976539634142
2216	4	-5.0996398168935215
2216	5	-4.4985090907457845
2216	6	-3.5089162499698716
2216	7	-5.301563910192794
2216	8	-3.2226867315483076
2216	9	-4.092571095725333
2216	10	-3.0005402446598985
2216	11	-3.1230537547283426
2216	12	-3.440308232673914
2216	13	-3.199579346093387
2216	14	-4.178142179482073
2216	15	-3.5134642255533137
2216	16	-3.7258196210995784
2216	17	-3.47992601577101
2216	18	-3.0543465097570497
2216	19	-3.694545451767844
2216	20	-4.259722844777137
2216	21	-3.945057993256775
2216	22	-3.6507135626811333
2216	23	-3.7351600132945677
2216	24	-4.250370550411706
2216	25	-4.078102705461035
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119260.0
3	4.0
4	1031.0
5	6474.0
6	8209.0
7	7523.0
8	6404.0
9	5900.0
10	5233.0
11	4988.0
12	4678.0
13	4391.0
14	4337.0
15	4406.0
16	4710.0
17	5063.0
18	5570.0
19	7002.0
20	9001.0
21	11689.0
22	14454.0
23	17509.0
24	20642.0
25	23089.0
26	25382.0
27	28086.0
28	31027.0
29	36806.0
30	44280.0
31	54328.0
32	68545.0
33	86976.0
34	112429.0
35	147838.0
36	201713.0
37	309888.0
38	892516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.846197362251935	19.183844371664136	12.891057250635779	24.07890101544815
2	13.949513929438076	20.033821404361415	43.94582766119308	22.07083700500743
3	19.296914743800848	26.03842442679228	28.825367861412705	25.839292967994165
4	11.692364601562764	16.561168232662382	38.943252962591664	32.80321420318319
5	12.18486300155697	39.724999305877404	35.31025144324106	12.779886249324566
6	30.76713632161141	37.48009740463745	18.718975994638214	13.033790279112928
7	28.15958884172381	32.05437657109078	21.78546438889404	18.00057019829137
8	24.92411092204161	38.250104748304786	20.488811170872275	16.33697315878133
9	27.67752111616455	14.937933864514585	20.505676572171115	36.87886844714974
10	15.723838808647361	29.377917860243656	33.93573258517555	20.96251074593343
11	33.88801471854921	22.68234787191887	25.381247272225195	18.04839013730673
12	24.404356021336348	25.048063213867515	31.75164710293287	18.795933661863266
13	29.384372459321355	22.92558798410214	26.011206129345815	21.67883342723069
14	20.496964505100195	23.680408255041417	27.918858541064463	27.903768698793925
15	22.29543206488518	33.353680483065276	23.407771414306442	20.94311603774311
16	20.657950307691518	28.752998688527953	29.82867934593965	20.760371657840874
17	20.46517434311642	29.879647051329243	28.824554720596552	20.830623884957784
18	20.228464081613982	27.444548144493545	32.448286904165904	19.878700869726565
19	23.04944134173743	26.90438855391813	28.619650680410537	21.426519423933897
20	23.480463050560815	27.469548128556	29.59387957248321	19.456109248399976
21	22.28168412053858	27.110066253472965	27.873007052789056	22.7352425731994
22	22.67223146410693	29.43994178681473	28.13826828599878	19.749558463079563
23	21.066280203149848	28.865917679850888	29.501701465483336	20.566100651515928
24	22.66365427939963	26.884701376093844	30.357126062849744	20.094518281656786
25	21.66931774122463	28.172622358027155	29.808665230626385	20.349394670121825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	304.0
1	304.0
2	853.5
3	1403.0
4	1403.0
5	1403.0
6	2649.0
7	3895.0
8	3895.0
9	3895.0
10	4871.5
11	5848.0
12	5848.0
13	5848.0
14	8240.5
15	10633.0
16	10633.0
17	10633.0
18	19375.0
19	28117.0
20	28117.0
21	28117.0
22	46164.5
23	64212.0
24	64212.0
25	64212.0
26	95907.0
27	127602.0
28	127602.0
29	127602.0
30	166854.5
31	206107.0
32	206107.0
33	206107.0
34	229427.5
35	252748.0
36	252748.0
37	252748.0
38	265613.5
39	278479.0
40	278479.0
41	278479.0
42	287691.0
43	296903.0
44	296903.0
45	296903.0
46	305699.5
47	314496.0
48	314496.0
49	314496.0
50	293704.0
51	272912.0
52	272912.0
53	272912.0
54	232974.0
55	193036.0
56	193036.0
57	193036.0
58	161592.0
59	130148.0
60	130148.0
61	130148.0
62	105839.0
63	81530.0
64	81530.0
65	81530.0
66	61825.0
67	42120.0
68	42120.0
69	42120.0
70	30694.0
71	19268.0
72	19268.0
73	19268.0
74	13391.5
75	7515.0
76	7515.0
77	7515.0
78	5173.5
79	2832.0
80	2832.0
81	2832.0
82	1871.0
83	910.0
84	910.0
85	910.0
86	582.5
87	255.0
88	255.0
89	255.0
90	165.0
91	75.0
92	75.0
93	75.0
94	50.5
95	26.0
96	26.0
97	26.0
98	16.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34283185863385757
2	0.011104557523957014
3	0.009139905038949235
4	0.011617075563524261
5	0.012642111642658757
6	0.07952571580618448
7	0.0787569387468336
8	0.10425471121530412
9	0.08289979290000218
10	0.0907156930034027
11	0.08627386999381989
12	0.09712216849799328
13	0.08315605191978581
14	0.08793955362241343
15	0.08486444538500997
16	0.0864874191769729
17	0.10011185706213556
18	0.10122231281453126
19	0.09417518977048162
20	0.09387622091406739
21	0.08033720270216595
22	0.10216192922040454
23	0.09485854715657127
24	0.09186885859242899
25	0.08875104051839491
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2341381.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84113061381153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11756097613284	45.18408047652886
2	12.950222126849646	14.98110979033965
3	4.194585176050635	7.2785864711610735
4	1.7656408521262816	4.0850665257967105
5	0.8844386395692263	2.55784654356127
6	0.5113735915882879	1.7747056022107244
7	0.33445072223225447	1.3541505535963603
8	0.2262320456427283	1.0468413840840656
9	0.16312233751298255	0.8491662387106805
>10	0.7520086551546636	7.728374978214212
>50	0.05493869094709433	2.1968132313760056
>100	0.03807161206134606	4.347305408852336
>500	0.0039372972626399326	1.558155734420037
>1k	0.0028972564762816786	2.8092522642131956
>5k	4.457317655817967E-4	1.7721432573752731
>10k+	7.428862759696611E-5	0.4764015395596638
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11087	0.47352395872350544	No Hit
GTCCTACAGTGGACATTTCTAAATT	9179	0.39203359043231323	No Hit
CTGTAGGACGTGGAATATGGCAAGA	7118	0.30400861713663857	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6855	0.2927759301027898	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6580	0.2810307250293737	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6065	0.25903515916461267	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5445	0.23255506045363827	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4923	0.21026052573246304	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3414	0.14581138225688173	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3334	0.14239459532643342	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3082	0.13163171649552122	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2376	0.10147857183431487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.270983663060391E-5	0.0
10	0.0	0.0	0.0	4.270983663060391E-5	0.0
11	0.0	0.0	0.0	4.270983663060391E-5	0.0
12	0.0	0.0	0.0	4.270983663060391E-5	0.0
13	0.0	0.0	0.0	4.270983663060391E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	120	5.2750693E-11	13.456289	10
TCCAACG	180	0.0	13.193005	18
TAGGACC	1365	0.0	13.082541	4
GGTATCA	2285	0.0	12.793185	1
CCAACGT	105	1.9988875E-8	12.665013	19
CGGTTTC	115	5.338734E-9	12.389688	13
TTGCGAG	85	3.9505376E-6	12.292776	18
GACAGCG	55	0.0030717517	12.08959	7
ACCGTGC	55	0.0030717517	12.08959	8
GGCGTGC	150	1.4551915E-11	12.03202	8
GCGTGCG	150	1.4551915E-11	12.031506	9
GCGTCGT	65	8.033431E-4	11.691032	6
ATACCGT	65	8.033431E-4	11.691032	6
AGGACCT	2395	0.0	11.620364	5
AGACCGT	90	7.474031E-6	11.609844	6
CCTACAC	400	0.0	11.398513	3
GGACCTG	2370	0.0	11.302565	6
TGTACGC	60	0.0058828737	11.081887	2
ATTTCTA	2870	0.0	11.02121	15
GTATCAA	4945	0.0	10.995028	1
>>END_MODULE