FastQCFastQC Report
Thu 26 May 2016
SRR1512864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341381
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130450.5571498188462279No Hit
TATCAACGCAGAGTACTTTTTTTTT93530.3994651020060383No Hit
GTCCTACAGTGGACATTTCTAAATT84650.36153876707806204No Hit
GGTATCAACGCAGAGTACTTTTTTT73410.31353291070526323No Hit
CTGTAGGACGTGGAATATGGCAAGA68250.2914946350038717No Hit
GTCCTAAAGTGTGTATTTCTCATTT63440.27095120358455116No Hit
CTTTAGGACGTGAAATATGGCGAGG58920.25164635742751823No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48130.2055624437030966No Hit
GTCCTACAGTGTGCATTTCTCATTT47260.20184668791623406No Hit
CTGTAGGACCTGGAATATGGCGAGA35970.15362728236028225No Hit
CTGAAGGACCTGGAATATGGCGAGA31540.13470682473292472No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26080.11138725393261498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.012.9159071
TAGGACC13650.012.6096934
GGCGAGG15750.012.29578719
CCCGTAC1552.5465852E-1111.63716316
GCGCCGC600.00590729511.07572117
GGCGCCG600.00590729511.07572116
CCGTACA1658.185452E-1110.93188217
TGTAGGA40650.010.8546542
GTCCTAC40750.010.7836521
AGGACCT24500.010.7515795
CAGGACT3100.010.7365544
ACGGAAT803.774563E-410.6867798
TCCAACG2850.010.65934218
GGACCTG23850.010.6458786
CCAACGA1701.4006218E-1010.60990119
GTAGGAC40400.010.56874753
GAACAGT4950.010.5661336
ATAGGAC3250.010.5336223
CTGTAGG40850.010.4778441
GACGTGG18200.010.3933257
Produced by FastQC (version 0.11.5)