##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2341381 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.214619064560615 31.0 30.0 34.0 26.0 34.0 2 30.238569032549595 31.0 30.0 34.0 26.0 34.0 3 30.407663682245648 31.0 30.0 34.0 26.0 34.0 4 33.977851105821735 37.0 35.0 37.0 30.0 37.0 5 33.56040430839748 37.0 35.0 37.0 28.0 37.0 6 33.40866480081627 37.0 35.0 37.0 28.0 37.0 7 33.241966172955195 37.0 35.0 37.0 26.0 37.0 8 33.18585569798337 37.0 35.0 37.0 26.0 37.0 9 34.474382853538145 39.0 35.0 39.0 26.0 39.0 10 34.40479101863387 39.0 35.0 39.0 26.0 39.0 11 34.371150188713415 39.0 35.0 39.0 26.0 39.0 12 34.24038377350803 39.0 35.0 39.0 25.0 39.0 13 34.223828159534904 39.0 35.0 39.0 25.0 39.0 14 35.30591219455526 40.0 36.0 41.0 25.0 41.0 15 35.31422267456685 40.0 36.0 41.0 25.0 41.0 16 35.32415484707529 40.0 36.0 41.0 25.0 41.0 17 35.33265367746642 40.0 36.0 41.0 25.0 41.0 18 35.26367045773413 40.0 36.0 41.0 25.0 41.0 19 35.288197862714355 40.0 36.0 41.0 25.0 41.0 20 35.26395704073792 40.0 36.0 41.0 25.0 41.0 21 35.23787926868801 40.0 36.0 41.0 25.0 41.0 22 35.202054684820624 40.0 36.0 41.0 25.0 41.0 23 35.048300981343914 40.0 35.0 41.0 24.0 41.0 24 35.01190664825588 40.0 35.0 41.0 24.0 41.0 25 34.957734772768724 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9324604312477902 1101 2 0.8193069679737235 1101 3 0.7038668262447096 1101 4 0.8026211638669949 1101 5 0.9241918329717933 1101 6 0.9763059675468213 1101 7 0.9771902598472195 1101 8 0.8163472979933957 1101 9 1.2151644505908763 1101 10 0.8600875174044518 1101 11 1.1474631452867428 1101 12 1.2038066838188897 1101 13 1.3613215712477071 1101 14 1.3489283565065335 1101 15 1.042732379821942 1101 16 1.252268344094162 1101 17 1.3209640238629845 1101 18 1.2415985133823568 1101 19 1.1531876213422834 1101 20 1.164072453255912 1101 21 1.2915874438996937 1101 22 1.217490107647663 1101 23 1.3557034270821617 1101 24 1.328894968057547 1101 25 1.4021734925884246 1102 1 0.6108669092645371 1102 2 0.753241239376166 1102 3 0.4277425579904204 1102 4 0.48779576666207447 1102 5 0.7010983568160469 1102 6 0.6585587680058964 1102 7 0.6937136657474099 1102 8 0.7802260583394514 1102 9 0.9060061225376685 1102 10 0.7444575486070022 1102 11 0.8779527822930575 1102 12 0.8518303228416144 1102 13 0.8829150932309631 1102 14 0.8660538959759734 1102 15 0.98569745926293 1102 16 0.9318838712499442 1102 17 0.9550146572788094 1102 18 0.29731081228926826 1102 19 0.650580235542435 1102 20 0.9120873252776178 1102 21 0.7658973414453314 1102 22 0.5870670201490071 1102 23 0.9226175622772885 1102 24 0.7957050947407112 1102 25 0.8446117549688239 1103 1 0.7657619203233104 1103 2 0.9536265227244591 1103 3 0.7137972441649474 1103 4 0.5617279086709104 1103 5 0.7856362790005278 1103 6 0.6770509805934637 1103 7 0.8292399368894152 1103 8 0.8872891750935707 1103 9 0.9815461824432177 1103 10 0.8285666926857047 1103 11 1.0149299583419378 1103 12 1.226061067157218 1103 13 1.0978982660038596 1103 14 1.1373681779885771 1103 15 1.2956397130317043 1103 16 1.2040632670738347 1103 17 1.2046245925919266 1103 18 1.3000697988922667 1103 19 1.2579402579993015 1103 20 1.024335572377197 1103 21 1.2454120818494943 1103 22 1.1747914731484457 1103 23 1.297992126172936 1103 24 1.2938662844611386 1103 25 1.1434899912409833 1104 1 0.7489795250943345 1104 2 0.8847784740675309 1104 3 0.7747595403513792 1104 4 0.6834939060974037 1104 5 0.7632323791508 1104 6 0.7061733588588766 1104 7 0.6881974862071019 1104 8 0.6820336130620532 1104 9 0.6460086080980219 1104 10 0.7359686695717116 1104 11 0.8664886721735243 1104 12 0.866671956591226 1104 13 0.7255240417085176 1104 14 0.8629302217040546 1104 15 0.9309037415934327 1104 16 0.8424218032790733 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2215 8 -1.5048335186526423 2215 9 -1.447607163844836 2215 10 -1.4970241052833302 2215 11 -1.2746153727347647 2215 12 -1.4333133451790232 2215 13 -1.4372967861284494 2215 14 -1.7237958663917894 2215 15 -1.6468758012916211 2215 16 -1.6308037380871596 2215 17 -1.7313498322811398 2215 18 -1.4547370294994693 2215 19 -1.7471883489502744 2215 20 -1.6490633703503974 2215 21 -1.3805757156389618 2215 22 -1.558200231651071 2215 23 -2.0398628227394937 2215 24 -1.9050179379421692 2215 25 -2.1307645899245173 2216 1 -1.4713718120569155 2216 2 -0.912380095957932 2216 3 -0.9114098832493873 2216 4 -1.1006573418607815 2216 5 -2.035117330095577 2216 6 -2.339651915021367 2216 7 -2.246024088176089 2216 8 -2.2498329898591223 2216 9 -4.113794962720366 2216 10 -2.1719600069334533 2216 11 -2.3003634726510143 2216 12 -2.668615743614936 2216 13 -2.63691405395236 2216 14 -2.7915467503344757 2216 15 -2.7961029052467126 2216 16 -2.044781751710026 2216 17 -2.39713060306201 2216 18 -2.7265044575335295 2216 19 -2.974422644497267 2216 20 -2.306289691119403 2216 21 -2.978591739511849 2216 22 -2.3495876143518046 2216 23 -3.452837557842521 2216 24 -2.8270993549944734 2216 25 -2.9585819875172206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68086.0 3 1.0 4 1190.0 5 9900.0 6 12494.0 7 9831.0 8 7950.0 9 6712.0 10 5692.0 11 5159.0 12 4302.0 13 3996.0 14 3992.0 15 3932.0 16 3887.0 17 4077.0 18 4563.0 19 5617.0 20 7448.0 21 10204.0 22 12717.0 23 15370.0 24 17544.0 25 20213.0 26 22385.0 27 24288.0 28 26796.0 29 32065.0 30 39781.0 31 50549.0 32 64322.0 33 82653.0 34 107871.0 35 142699.0 36 195627.0 37 306798.0 38 1000670.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94384630576576 20.077040337811322 13.892824074271982 26.086289282150936 2 12.639872838989207 20.59914371416119 42.551680525051914 24.209302921797686 3 17.400438541595026 25.9206874500468 29.58647825810219 27.09239575025599 4 10.969796242629519 17.23264987090312 38.11110660262722 33.686447283840145 5 11.8815044791013 39.857401783780574 35.307475534850106 12.953618202268022 6 30.891587725828636 37.888311570648455 18.262022327087298 12.958078376435614 7 27.814970920106052 32.247031423040546 21.82968888980073 18.108308767052677 8 24.867021958469145 38.64464951398064 20.094255451159626 16.394073076390587 9 26.92402657040945 14.863153847796834 20.47707127448258 37.735748307311134 10 15.1718748197375 30.026573096096843 33.747148380767385 21.054403703398272 11 33.54656645834543 22.800411403110125 25.304708242071776 18.34831389647266 12 23.862687523357 25.377139501361377 31.772441407292725 18.987731567988895 13 29.009551422291434 22.91435839916078 26.11802725243484 21.958062926112945 14 20.218094254952117 23.6325011008498 28.022318528487343 28.127086115710735 15 22.04614120872385 33.44569123604087 23.441936480123502 21.066231075111777 16 20.635104374944206 28.804332272448267 29.578532048880728 20.982031303726796 17 20.150944685649364 29.984474138869967 28.899628617984796 20.964952557495874 18 20.09877726508365 27.538850354963447 32.44666151552156 19.915710864431343 19 22.856953710268478 26.887508862446293 28.832634303433075 21.42290312385215 20 23.308605613729075 27.434571342297044 29.69359352890854 19.563229515065345 21 21.856317091900582 26.888097522057524 28.3119171701451 22.943668215896793 22 22.532239616567022 29.15386435486172 28.453207390190542 19.86068863838071 23 20.717132324897143 28.759736241132906 29.76234965603633 20.76078177793362 24 22.515600835575245 26.805590375936255 30.509344698705593 20.16946408978291 25 21.39815894820958 28.056500036944037 29.978401617166632 20.566939397679743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 123.0 1 123.0 2 471.0 3 819.0 4 819.0 5 819.0 6 2132.5 7 3446.0 8 3446.0 9 3446.0 10 4426.0 11 5406.0 12 5406.0 13 5406.0 14 7891.5 15 10377.0 16 10377.0 17 10377.0 18 19726.0 19 29075.0 20 29075.0 21 29075.0 22 47706.5 23 66338.0 24 66338.0 25 66338.0 26 99106.5 27 131875.0 28 131875.0 29 131875.0 30 170024.0 31 208173.0 32 208173.0 33 208173.0 34 230540.5 35 252908.0 36 252908.0 37 252908.0 38 265507.0 39 278106.0 40 278106.0 41 278106.0 42 288168.0 43 298230.0 44 298230.0 45 298230.0 46 306945.0 47 315660.0 48 315660.0 49 315660.0 50 294136.5 51 272613.0 52 272613.0 53 272613.0 54 231607.0 55 190601.0 56 190601.0 57 190601.0 58 159052.5 59 127504.0 60 127504.0 61 127504.0 62 103865.0 63 80226.0 64 80226.0 65 80226.0 66 60525.0 67 40824.0 68 40824.0 69 40824.0 70 29595.0 71 18366.0 72 18366.0 73 18366.0 74 12704.5 75 7043.0 76 7043.0 77 7043.0 78 4839.0 79 2635.0 80 2635.0 81 2635.0 82 1691.0 83 747.0 84 747.0 85 747.0 86 486.5 87 226.0 88 226.0 89 226.0 90 139.0 91 52.0 92 52.0 93 52.0 94 29.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08913542904807036 2 0.044460939932458664 3 0.018792328117465718 4 0.004954341049150053 5 0.016998514978980354 6 0.06163029425796143 7 0.0765360272420422 8 0.048902762942041475 9 0.018407939587790283 10 0.04458906944235048 11 0.005338729578825488 12 2.562590197836234E-4 13 0.006790864024266021 14 3.4167869304483126E-4 15 0.014948442820711366 16 0.010848298504173392 17 0.005381439415456092 18 0.006577314841113 19 0.00175110330185476 20 5.979377128284547E-4 21 1.281295098918117E-4 22 2.9896885641422734E-4 23 0.0 24 0.0 25 8.541967326120781E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2341381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.95075053947842 #Duplication Level Percentage of deduplicated Percentage of total 1 77.27320661547527 44.00767113343506 2 13.581725692892938 15.469789436631407 3 4.287286322242672 7.3249252148808095 4 1.836913669314291 4.184544485747046 5 0.8950114337934035 2.5485786447974514 6 0.5310548712696453 1.8146384097871437 7 0.34827054011086994 1.3883988055072496 8 0.21975386993580967 1.0012118261439427 9 0.16056555120819188 0.8229895786882426 >10 0.7650601950633169 7.891637139331248 >50 0.054868179280062275 2.1711649922062803 >100 0.03876282277677488 4.384655661429396 >500 0.003985725417519732 1.535095189531866 >1k 0.0030080946547312313 3.0021361911837023 >5k 4.512141982096847E-4 1.8938679597435455 >10k+ 7.520236636828078E-5 0.5586953309555529 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13045 0.5571498188462279 No Hit TATCAACGCAGAGTACTTTTTTTTT 9353 0.3994651020060383 No Hit GTCCTACAGTGGACATTTCTAAATT 8465 0.36153876707806204 No Hit GGTATCAACGCAGAGTACTTTTTTT 7341 0.31353291070526323 No Hit CTGTAGGACGTGGAATATGGCAAGA 6825 0.2914946350038717 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6344 0.27095120358455116 No Hit CTTTAGGACGTGAAATATGGCGAGG 5892 0.25164635742751823 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4813 0.2055624437030966 No Hit GTCCTACAGTGTGCATTTCTCATTT 4726 0.20184668791623406 No Hit CTGTAGGACCTGGAATATGGCGAGA 3597 0.15362728236028225 No Hit CTGAAGGACCTGGAATATGGCGAGA 3154 0.13470682473292472 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2608 0.11138725393261498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.270983663060391E-5 0.0 10 0.0 0.0 0.0 4.270983663060391E-5 0.0 11 0.0 0.0 0.0 4.270983663060391E-5 0.0 12 0.0 0.0 0.0 4.270983663060391E-5 0.0 13 0.0 0.0 0.0 4.270983663060391E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2305 0.0 12.915907 1 TAGGACC 1365 0.0 12.609693 4 GGCGAGG 1575 0.0 12.295787 19 CCCGTAC 155 2.5465852E-11 11.637163 16 GCGCCGC 60 0.005907295 11.075721 17 GGCGCCG 60 0.005907295 11.075721 16 CCGTACA 165 8.185452E-11 10.931882 17 TGTAGGA 4065 0.0 10.854654 2 GTCCTAC 4075 0.0 10.783652 1 AGGACCT 2450 0.0 10.751579 5 CAGGACT 310 0.0 10.736554 4 ACGGAAT 80 3.774563E-4 10.686779 8 TCCAACG 285 0.0 10.659342 18 GGACCTG 2385 0.0 10.645878 6 CCAACGA 170 1.4006218E-10 10.609901 19 GTAGGAC 4040 0.0 10.5687475 3 GAACAGT 495 0.0 10.566133 6 ATAGGAC 325 0.0 10.533622 3 CTGTAGG 4085 0.0 10.477844 1 GACGTGG 1820 0.0 10.393325 7 >>END_MODULE