FastQCFastQC Report
Thu 26 May 2016
SRR1512863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160679
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79950.37002257160827684No Hit
CTGTAGGACGTGGAATATGGCAAGA64520.29860983514904343No Hit
GTCCTAAAGTGTGTATTTCTCATTT63410.29347256117174275No Hit
GTATCAACGCAGAGTACTTTTTTTT54970.2544107662452405No Hit
CTTTAGGACGTGAAATATGGCGAGG51410.23793446411984379No Hit
GTCCTACAGTGTGCATTTCTCATTT43800.2027140542394312No Hit
TATCAACGCAGAGTACTTTTTTTTT33090.1531463026206114No Hit
GGTATCAACGCAGAGTACTTTTTTT31190.14435277058739404No Hit
CTGTAGGACCTGGAATATGGCGAGA30320.1403262585511314No Hit
CTGAAGGACCTGGAATATGGCGAGA26720.12366482943556169No Hit
GTACTGGTTCACTATCGGTCAGTCA26210.12130446031085598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2250.013.93137919
TCCAACG3350.013.89389718
TAGGACC12450.012.5885964
TAAGACG701.0927087E-412.2125744
GGTATCA18850.012.0750021
TAGACAG1800.011.6092165
ACTGTTC8250.011.5135378
CGCCCTA4000.011.39866616
CCAACGT1354.7421054E-911.25768219
TGTAGGA36750.011.0882422
TGTAGAA10200.011.081782
GATATAC7400.011.0679561
AGGACCT21050.010.9649545
GTATTAA2000.010.9521271
GTCCTAC35500.010.9185931
CTGTAGG35950.010.9143781
CCGTTTC2700.010.90587913
GGACCTG20650.010.8096226
GTATAGG2300.010.7657971
CCCGTTT2650.010.75320912
Produced by FastQC (version 0.11.5)