##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512863_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2160679
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.835291128390658	31.0	30.0	34.0	20.0	34.0
2	28.977594543196837	31.0	30.0	34.0	18.0	34.0
3	29.067563020698586	31.0	30.0	34.0	22.0	34.0
4	32.43412649449548	35.0	33.0	37.0	22.0	37.0
5	32.63383177232713	35.0	33.0	37.0	25.0	37.0
6	32.50422806904681	36.0	33.0	37.0	23.0	37.0
7	32.47671218167993	37.0	33.0	37.0	23.0	37.0
8	32.38246588225275	36.0	33.0	37.0	22.0	37.0
9	33.670523016144465	38.0	34.0	39.0	20.0	39.0
10	33.5240672029487	38.0	34.0	39.0	18.0	39.0
11	33.61227975094866	38.0	34.0	39.0	19.0	39.0
12	33.50312656345528	38.0	34.0	39.0	18.0	39.0
13	33.49370591374286	38.0	34.0	39.0	18.0	39.0
14	34.32148412605482	39.0	34.0	41.0	17.0	41.0
15	34.52245520968177	39.0	34.0	41.0	17.0	41.0
16	34.46390787340461	39.0	34.0	41.0	17.0	41.0
17	34.22362183369209	39.0	34.0	41.0	17.0	41.0
18	34.22417027240048	39.0	34.0	41.0	17.0	41.0
19	34.34174766358168	39.0	34.0	41.0	18.0	41.0
20	34.316203378660134	39.0	34.0	41.0	17.0	41.0
21	34.20582927866657	39.0	34.0	41.0	11.0	41.0
22	34.2155965786681	39.0	34.0	41.0	11.0	41.0
23	33.882459634216836	39.0	34.0	41.0	8.0	41.0
24	33.95779382314541	39.0	34.0	41.0	2.0	41.0
25	33.665643531500976	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7925452400066533
1101	2	0.5289316468042635
1101	3	0.9198696497754923
1101	4	1.0347100955728692
1101	5	0.8933284052154704
1101	6	0.9214654343732036
1101	7	0.6984259085126752
1101	8	0.9990244317266104
1101	9	0.9990948933969221
1101	10	0.9392190220277143
1101	11	0.9732292678632319
1101	12	0.7654438470036453
1101	13	1.166019209493328
1101	14	1.3568826728197294
1101	15	1.1685773483356172
1101	16	1.0239938514061109
1101	17	0.4751617574275002
1101	18	0.9443389250143426
1101	19	0.47462677791853025
1101	20	-0.09056103745088251
1101	21	0.9427916626651722
1101	22	1.0029666325674782
1101	23	0.6481167021856962
1101	24	1.1370905050222575
1101	25	0.7674622449019282
1102	1	1.0629874613368244
1102	2	1.0769626208200833
1102	3	1.1061977502008915
1102	4	1.218263588115775
1102	5	1.1026206683921558
1102	6	0.9354702362532876
1102	7	1.107577391332235
1102	8	1.097952335160791
1102	9	1.3700581461788843
1102	10	1.4186691411203398
1102	11	1.257124407921964
1102	12	1.4454625411080713
1102	13	1.3591721772457106
1102	14	1.5581390810235547
1102	15	1.540933407334819
1102	16	1.5775011968958665
1102	17	1.7414022849915654
1102	18	1.6945675372780116
1102	19	1.4132634483189506
1102	20	1.5752749316187291
1102	21	1.7433556800395067
1102	22	1.4949573638131426
1102	23	1.4588133799211889
1102	24	0.9705207368443425
1102	25	1.3183641409764917
1103	1	0.7810441663914958
1103	2	0.8459303360575241
1103	3	0.9323758466607011
1103	4	1.1869765823571967
1103	5	0.8430426961920432
1103	6	0.5072863470765512
1103	7	0.42642754157419205
1103	8	0.6823294474049177
1103	9	0.7661094681639753
1103	10	1.0319635630131074
1103	11	0.7667887664028399
1103	12	0.8723312916153887
1103	13	1.0351525050697887
1103	14	1.0102300652164757
1103	15	0.975571233563933
1103	16	0.9768457232107792
1103	17	0.627709310367706
1103	18	0.9884632418221386
1103	19	0.9352462438889972
1103	20	1.0459707328092875
1103	21	0.8997708902454917
1103	22	0.9324750488201232
1103	23	0.13647231360913992
1103	24	0.3686665705411727
1103	25	0.6254868083572589
1104	1	1.0879605458697128
1104	2	1.1034077122730217
1104	3	0.982383382326411
1104	4	1.2021585239177384
1104	5	0.9997371652372422
1104	6	0.9731979052209283
1104	7	1.0887323510449534
1104	8	0.8866429818335817
1104	9	1.1132560435769676
1104	10	1.2348945639211166
1104	11	0.9721361232867451
1104	12	1.2571157193487252
1104	13	1.2207529670583241
1104	14	1.4500141273581093
1104	15	1.1813479741721409
1104	16	1.2818997300585337
1104	17	1.5515622477478104
1104	18	1.3652397190551042
1104	19	0.9518477444564084
1104	20	1.0892922304221244
1104	21	1.2312626797273438
1104	22	1.0435808853797468
1104	23	1.4189573747114537
1104	24	1.3406489094775083
1104	25	0.9472300764337191
1105	1	0.7195221798352591
1105	2	0.7536512979776084
1105	3	0.8350291790749047
1105	4	0.8515582137081878
1105	5	0.6234411397307156
1105	6	0.8229501091896552
1105	7	0.8735251636145733
1105	8	0.8974583042741315
1105	9	0.533125563722372
1105	10	0.7391803103726815
1105	11	0.9081351303818792
1105	12	0.954346367817756
1105	13	0.9426023109521324
1105	14	1.0670199297374552
1105	15	0.983038749840631
1105	16	0.9861464303065262
1105	17	1.2719142713315037
1105	18	1.1779763783146464
1105	19	0.9440040888970103
1105	20	1.0479984446263373
1105	21	0.8794657214504014
1105	22	0.8646176545288142
1105	23	1.1527662430873065
1105	24	1.1603606155952448
1105	25	1.3719095287031706
1106	1	0.7898834322601012
1106	2	0.8036528004600427
1106	3	0.7412664844895183
1106	4	0.7447040743319846
1106	5	0.6767139731046754
1106	6	0.7842125876772599
1106	7	0.6964232927086087
1106	8	0.8482325279080669
1106	9	0.8564759214801327
1106	10	0.5912594307267227
1106	11	0.6390160413981931
1106	12	0.7327725007729811
1106	13	0.8141207716384784
1106	14	1.083479903048918
1106	15	0.7712697863791433
1106	16	0.8741150332464187
1106	17	1.0517840858658118
1106	18	1.0589352514750772
1106	19	1.0147565432298933
1106	20	1.096827083351279
1106	21	1.1135404278449244
1106	22	0.9256373615336102
1106	23	1.2113804942630466
1106	24	0.8342774092309497
1106	25	1.1024563692422973
1107	1	0.9519297193063885
1107	2	1.0082577193506772
1107	3	0.9725827872343906
1107	4	1.1362886802023056
1107	5	0.969094211913486
1107	6	0.9412460304249848
1107	7	0.7451721628860497
1107	8	1.1118558093884943
1107	9	0.9482710311323856
1107	10	1.1905173156988909
1107	11	0.9339056755735413
1107	12	0.988891381891861
1107	13	0.8553176699782341
1107	14	1.180252178392621
1107	15	0.9588381703761044
1107	16	1.0092418112303392
1107	17	1.4680107163781528
1107	18	1.300610243150068
1107	19	1.090520065421984
1107	20	1.254264883646222
1107	21	0.969571301732941
1107	22	1.0516482554016449
1107	23	1.3367967446792974
1107	24	1.1623600408850123
1107	25	1.4612344958775694
1108	1	0.7887027947158245
1108	2	0.7725767336696912
1108	3	0.8326393972910004
1108	4	0.8492045522090805
1108	5	0.7310935761855717
1108	6	0.7449423341212906
1108	7	0.7371271775683326
1108	8	0.739065720689311
1108	9	0.8733421721126149
1108	10	0.8877821720546564
1108	11	0.6076448152217679
1108	12	0.7776367274553877
1108	13	0.8236220928281099
1108	14	0.9587539796216973
1108	15	0.8749410069334829
1108	16	0.6054637710886652
1108	17	0.8566977868834087
1108	18	1.0813673648162805
1108	19	0.9969204831860381
1108	20	1.0116665728660976
1108	21	1.0348734238532487
1108	22	1.0310514279866396
1108	23	1.2563431074074742
1108	24	1.1455018990268684
1108	25	1.2824473739086244
1109	1	0.6044053426565021
1109	2	0.5840518651622553
1109	3	0.7542060642092068
1109	4	0.8143855204141914
1109	5	0.6627941058603284
1109	6	0.7611893062078892
1109	7	0.6712495234491698
1109	8	0.5664681397271494
1109	9	0.753726301215778
1109	10	0.8568230223880988
1109	11	0.770388943419384
1109	12	0.6863671339143878
1109	13	0.7920474132158191
1109	14	0.829190156935482
1109	15	0.7432158835016338
1109	16	0.42644111396131024
1109	17	0.8086641555208445
1109	18	0.9067321379122859
1109	19	0.7001230568524051
1109	20	0.5950104251471373
1109	21	0.5898209194404345
1109	22	0.586801050505052
1109	23	0.7395869382161493
1109	24	0.6938636186552927
1109	25	0.3181048916061826
1110	1	0.6387334442680057
1110	2	0.6574846422618101
1110	3	0.6268026309279193
1110	4	0.6473206543921677
1110	5	0.5864014068522536
1110	6	0.5473716260242654
1110	7	0.46799824623844444
1110	8	0.5285379773864491
1110	9	0.6865439838790195
1110	10	0.6824436918357932
1110	11	0.5787579901626998
1110	12	0.5314534784013247
1110	13	0.6697294317584124
1110	14	0.7792705379373004
1110	15	0.7042359328791292
1110	16	0.8412475011307663
1110	17	0.9152345688651664
1110	18	0.20116613581122778
1110	19	0.5777473829124631
1110	20	0.800188945359757
1110	21	0.8724342401221961
1110	22	0.7529473001217397
1110	23	0.8458869077168245
1110	24	0.758096345831845
1110	25	0.9350005235909649
1111	1	0.341928709183005
1111	2	0.37091886087735304
1111	3	0.4722441777682249
1111	4	0.3281799063578532
1111	5	0.4186282295802357
1111	6	0.17490669742858245
1111	7	0.09645028777488562
1111	8	0.23612305044957083
1111	9	0.3467293848527575
1111	10	0.3013550335046489
1111	11	0.12023425964903112
1111	12	-0.2019184920809849
1111	13	0.22805521173390986
1111	14	0.2911195958393691
1111	15	0.23145411139832106
1111	16	0.3235234121517294
1111	17	-0.14489758656758056
1111	18	0.17521819110958603
1111	19	0.22802059397491803
1111	20	0.3146648925057747
1111	21	0.28669962163264273
1111	22	-0.007757654457762442
1111	23	0.37778602461904853
1111	24	0.3053444319618279
1111	25	0.4126385841589908
1112	1	0.19409074910682023
1112	2	0.19877599908828714
1112	3	0.17182716119995334
1112	4	0.32503921096027
1112	5	0.3028510684398569
1112	6	0.04021397697657392
1112	7	-0.11467090606483055
1112	8	0.08613137892428568
1112	9	-0.019429638705830143
1112	10	0.028309015944124383
1112	11	0.03431750042774695
1112	12	0.0985925543253785
1112	13	0.04074933868127317
1112	14	0.1274038495219898
1112	15	-0.10853884542098768
1112	16	0.24329957502788346
1112	17	0.47671514701567475
1112	18	0.15556167338031202
1112	19	0.2181001990430147
1112	20	0.008339805007388179
1112	21	-0.024039022267714927
1112	22	-0.12007794281154105
1112	23	-0.0472109762243349
1112	24	0.14486777478679613
1112	25	0.2374030299457317
1113	1	-0.2277799588100109
1113	2	-0.1079743490830154
1113	3	-0.05966094431408919
1113	4	0.02795109272219065
1113	5	-0.1321717006212566
1113	6	-0.5116828483439875
1113	7	-0.3379342826190097
1113	8	-0.21185471145412293
1113	9	-0.29513740477315054
1113	10	-0.3109750119811565
1113	11	-0.28731367093549665
1113	12	-0.5906500210852315
1113	13	-0.3424089950137841
1113	14	-0.09428749591530305
1113	15	-0.38714787332141043
1113	16	-0.42123327567580304
1113	17	-0.20433624913219006
1113	18	-0.506300787109744
1113	19	-0.8763087315053042
1113	20	-0.6157547259446474
1113	21	-0.3132217549593008
1113	22	-0.8518882910955057
1113	23	-4.311868466123805
1113	24	-1.8770748868755263
1113	25	-1.0947632433472876
1114	1	-0.6243860143684294
1114	2	-0.8808204954060344
1114	3	-0.743147550625384
1114	4	-0.6283023307352629
1114	5	-0.6107891093581443
1114	6	-1.125987362486903
1114	7	-1.0707271430967644
1114	8	-1.0282359186468675
1114	9	-1.053422560592523
1114	10	-0.8387554296490123
1114	11	-0.9596182500485071
1114	12	-1.7558808083396187
1114	13	-0.9651916745394615
1114	14	-2.7907265645067874
1114	15	-1.662438865044983
1114	16	-1.6870003599596402
1114	17	-1.4490725483042723
1114	18	-1.4669845489749918
1114	19	-1.461971023864507
1114	20	-1.044388446322344
1114	21	-1.1101792365248997
1114	22	-1.2580051218404549
1114	23	-5.51363283617826
1114	24	-2.6702194360055316
1114	25	-2.6216609489698612
1115	1	-1.722129001014416
1115	2	-1.546603659558457
1115	3	-1.290524959670968
1115	4	-1.212068287824934
1115	5	-1.3097718197240091
1115	6	-1.3512198891017633
1115	7	-1.8884261414058336
1115	8	-2.2309325610434243
1115	9	-3.424371794111348
1115	10	-4.143238089403869
1115	11	-2.8986956580937395
1115	12	-3.0027177054392276
1115	13	-2.3540272772859865
1115	14	-2.974965874352435
1115	15	-2.625720794441534
1115	16	-2.7565341978657365
1115	17	-2.6346753033061603
1115	18	-4.990823687709671
1115	19	-3.2010717282306373
1115	20	-3.5478735691460095
1115	21	-3.204978731330449
1115	22	-2.8238023078687
1115	23	-3.027139276661501
1115	24	-3.021396722533492
1115	25	-3.318529018038877
1116	1	-1.665130109522945
1116	2	-1.51481823564459
1116	3	-1.8446876512167059
1116	4	-1.5805481268154615
1116	5	-1.7068659522178464
1116	6	-3.9018251671404265
1116	7	-2.6268555671215097
1116	8	-2.861188216199288
1116	9	-3.9869410548139044
1116	10	-3.2944390411055835
1116	11	-3.0389421406071158
1116	12	-2.8300763975846692
1116	13	-4.088012212835483
1116	14	-7.038179024538728
1116	15	-4.981372847222932
1116	16	-3.813807728965447
1116	17	-4.932887455952827
1116	18	-3.5650250362348395
1116	19	-3.3253940041927663
1116	20	-4.097696415215104
1116	21	-4.894025560714407
1116	22	-4.334787554906903
1116	23	-3.3597809656554602
1116	24	-4.335550199275303
1116	25	-4.0747851430418045
1201	1	0.6143640734178177
1201	2	0.5724927764027221
1201	3	0.9035670642523961
1201	4	0.8489012810097805
1201	5	0.45302743983484106
1201	6	0.8845223044320534
1201	7	0.867333210250095
1201	8	0.9123430849694643
1201	9	1.2859749938363478
1201	10	1.1318797217708507
1201	11	1.3170929390699868
1201	12	1.2317504123055443
1201	13	1.3115491337050074
1201	14	1.3770591711084208
1201	15	1.2945514629132546
1201	16	1.2481549904932976
1201	17	1.5157402094543073
1201	18	1.214025008688509
1201	19	1.4272376108372242
1201	20	1.4436897623278142
1201	21	1.5408763500055116
1201	22	1.3895913605995887
1201	23	1.7277122782400482
1201	24	1.6448671496413354
1201	25	1.821009090427303
1202	1	0.9714701307490721
1202	2	0.910862080774951
1202	3	0.9620166273135915
1202	4	1.1177373687806806
1202	5	0.8980654404391046
1202	6	1.1482661154413876
1202	7	1.1276130676072214
1202	8	1.1975944663471907
1202	9	1.3283843361842287
1202	10	1.1634271143616104
1202	11	1.1427802111997636
1202	12	1.4225529108975792
1202	13	1.339711272687751
1202	14	1.7353620449653064
1202	15	1.503109843181889
1202	16	1.1834888917466273
1202	17	1.6047597489477141
1202	18	1.5808702924635938
1202	19	1.5215242947513516
1202	20	1.27747891607968
1202	21	1.4589365513163841
1202	22	1.4866429114319502
1202	23	1.5015820976176926
1202	24	1.6042320134115613
1202	25	1.864863058464337
1203	1	1.03793857024462
1203	2	1.1424424375083753
1203	3	0.9994240250059327
1203	4	1.2286549159520206
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2211	18	-0.09228650530386773
2211	19	0.06279709007331036
2211	20	-0.0447421957195715
2211	21	0.22758733499890127
2211	22	0.043507310293634305
2211	23	0.1715653647684121
2211	24	-0.28196500337387675
2211	25	0.13665981298915142
2212	1	-0.221907605801146
2212	2	-0.12724255789325056
2212	3	-0.4425698045925657
2212	4	-0.2953424292579072
2212	5	-0.4515500975876421
2212	6	-0.555352348101799
2212	7	-0.45107885322639873
2212	8	-0.4221274321582591
2212	9	-0.286484590459267
2212	10	-0.48853650192305764
2212	11	-0.7103318228787359
2212	12	-0.19757601906745492
2212	13	-0.29106914182428767
2212	14	-0.32440646165862574
2212	15	-0.5164938494131945
2212	16	-0.6273055328805128
2212	17	-0.3512464314009307
2212	18	-0.21738369516719303
2212	19	-0.3951002611336776
2212	20	-0.27624675252361186
2212	21	-0.18075277757496622
2212	22	-0.11538784552590897
2212	23	-0.15925051060583684
2212	24	-0.17482442591839487
2212	25	-0.024702777011206933
2213	1	-0.29843984826012715
2213	2	-0.1672325931969354
2213	3	-0.34376503616785214
2213	4	-0.2577688411227115
2213	5	-0.13622348363600167
2213	6	0.026044042663251332
2213	7	-0.23409966903479074
2213	8	-0.1419362642639328
2213	9	-0.6351306784930486
2213	10	-0.17719377156301874
2213	11	-0.2650234426256617
2213	12	-0.3887886331693835
2213	13	-0.39100611852811085
2213	14	-0.4151861916642332
2213	15	-0.37177076622909055
2213	16	-0.24226985015751268
2213	17	-0.38532373111119966
2213	18	-0.3871198451702895
2213	19	-0.4373983521811411
2213	20	-0.09765874699897381
2213	21	-0.34647054824930024
2213	22	-0.5539713026005799
2213	23	-0.39560873495480564
2213	24	-0.16341456602262383
2213	25	-0.07109305995272308
2214	1	-2.6532199828433143
2214	2	-1.2033972923043343
2214	3	-1.5315000928484892
2214	4	-3.2405566966430754
2214	5	-1.6007356249163536
2214	6	-1.502574741395783
2214	7	-1.2539632942494094
2214	8	-1.0529504584177722
2214	9	-0.868691672830316
2214	10	-0.8997991826176062
2214	11	-0.8900350133671466
2214	12	-0.7882510682505881
2214	13	-1.2645269298663706
2214	14	-1.0788511627107198
2214	15	-1.3385011199631904
2214	16	-1.069128559140033
2214	17	-3.4298903455229066
2214	18	-3.178603950064126
2214	19	-1.9633895434876223
2214	20	-1.7759345465532093
2214	21	-1.7643621352004928
2214	22	-2.720167077413688
2214	23	-1.6306704917879387
2214	24	-2.7078862683612996
2214	25	-2.6740493684062976
2215	1	-2.100447426472247
2215	2	-3.5129769319954995
2215	3	-2.1924851064580366
2215	4	-3.672387154495837
2215	5	-2.245905008197262
2215	6	-2.0635892829605105
2215	7	-2.327138735633028
2215	8	-1.670231217587009
2215	9	-3.9287805108819818
2215	10	-2.2213335348136702
2215	11	-1.9176413214195556
2215	12	-1.8336350693520629
2215	13	-1.9855589255452024
2215	14	-1.7033081893684923
2215	15	-1.907223432209662
2215	16	-2.0698259611459804
2215	17	-2.203582250753847
2215	18	-1.9708323786642978
2215	19	-1.990078239618697
2215	20	-1.9070162144378457
2215	21	-2.5416255249843296
2215	22	-2.332545772379742
2215	23	-2.257391130642411
2215	24	-2.1305111214099455
2215	25	-3.0491282826078887
2216	1	-3.151114785208705
2216	2	-3.1877110177945305
2216	3	-4.462713289238156
2216	4	-5.073983400658015
2216	5	-4.742315456849127
2216	6	-3.697583767228341
2216	7	-5.381633384507733
2216	8	-3.283409219700065
2216	9	-4.016265902522196
2216	10	-3.0925486379553
2216	11	-3.3076571733422497
2216	12	-3.737433763550932
2216	13	-3.272164651621736
2216	14	-4.2989930861467585
2216	15	-3.7549916272772244
2216	16	-4.043139141150615
2216	17	-3.701764606338344
2216	18	-3.230776997060701
2216	19	-3.9289482113992307
2216	20	-4.398934857788813
2216	21	-4.126224569062927
2216	22	-3.761924936112724
2216	23	-3.6995693402162004
2216	24	-4.264136501788002
2216	25	-4.189129355956872
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115099.0
3	4.0
4	978.0
5	6212.0
6	7334.0
7	6533.0
8	5739.0
9	5129.0
10	4725.0
11	4477.0
12	4205.0
13	4009.0
14	4184.0
15	4122.0
16	4266.0
17	4533.0
18	5367.0
19	6427.0
20	8243.0
21	10730.0
22	13332.0
23	15884.0
24	18581.0
25	21020.0
26	23451.0
27	26007.0
28	29146.0
29	33998.0
30	41629.0
31	51762.0
32	64417.0
33	81519.0
34	106278.0
35	139505.0
36	188831.0
37	286462.0
38	806541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42332660327909	18.437175531825975	12.672716904942781	24.466780959952153
2	14.422809438266945	19.532419105561416	43.182103266974906	22.862668189196732
3	19.830517418065984	25.08634743188713	28.454846148362982	26.628289001683903
4	12.218517205339298	16.086721464298375	37.75259970913252	33.94216162122981
5	12.852954490961421	39.185690097139144	34.80114385907093	13.160211552828496
6	32.06910846833493	36.16971409381984	18.30448023900784	13.456697198837384
7	28.99451396428611	31.3535926956612	21.11447490801024	18.53741843204245
8	25.619975232031756	37.60306666919937	20.06488040166176	16.712077697107112
9	27.892216489735723	14.748481364580893	19.865844993112063	37.49345715257133
10	16.403059794590003	28.745772353543114	33.330692863830066	21.52047498803682
11	35.01936963791307	21.883637647183075	24.641292278872758	18.455700436031094
12	24.84723796040556	24.07826234396035	31.200656355595747	19.87384334003834
13	29.97421789813337	22.001267334364126	25.381613090600514	22.642901676901996
14	21.02646652136754	22.87923882404986	27.00632074357329	29.0879739110093
15	23.177350523357585	32.27789437797641	22.660639998443617	21.884115100222388
16	21.68247416949812	27.763441009641998	28.8799486748333	21.674136146026584
17	21.440322068800974	28.88529078615456	27.93650352436479	21.73788362067967
18	21.212974781039566	26.930152722151313	31.085918660918832	20.770953835890285
19	23.797682039745727	26.31739137282952	27.434075998484218	22.450850588940536
20	24.429462983654734	26.909001291537187	28.233243833697447	20.42829189111063
21	23.33909241237476	26.46724504495324	26.66062966960179	23.533032873070205
22	23.7592882832005	28.66427086600457	26.699850219990832	20.87659063080409
23	22.33820585017034	28.08326361344514	27.906253850807776	21.672276685576737
24	23.72958221982574	26.36461716416078	28.658709169651075	21.247091446362404
25	22.702062442588332	27.40329940785204	28.373675333560932	21.520962815998697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	257.0
1	257.0
2	608.5
3	960.0
4	960.0
5	960.0
6	1592.5
7	2225.0
8	2225.0
9	2225.0
10	2610.0
11	2995.0
12	2995.0
13	2995.0
14	4514.5
15	6034.0
16	6034.0
17	6034.0
18	12164.5
19	18295.0
20	18295.0
21	18295.0
22	32410.5
23	46526.0
24	46526.0
25	46526.0
26	73076.0
27	99626.0
28	99626.0
29	99626.0
30	133657.0
31	167688.0
32	167688.0
33	167688.0
34	188999.5
35	210311.0
36	210311.0
37	210311.0
38	223862.0
39	237413.0
40	237413.0
41	237413.0
42	251096.0
43	264779.0
44	264779.0
45	264779.0
46	279597.5
47	294416.0
48	294416.0
49	294416.0
50	282011.5
51	269607.0
52	269607.0
53	269607.0
54	235691.0
55	201775.0
56	201775.0
57	201775.0
58	174199.0
59	146623.0
60	146623.0
61	146623.0
62	121969.5
63	97316.0
64	97316.0
65	97316.0
66	74970.5
67	52625.0
68	52625.0
69	52625.0
70	38833.0
71	25041.0
72	25041.0
73	25041.0
74	17746.0
75	10451.0
76	10451.0
77	10451.0
78	7213.5
79	3976.0
80	3976.0
81	3976.0
82	2616.5
83	1257.0
84	1257.0
85	1257.0
86	810.0
87	363.0
88	363.0
89	363.0
90	230.0
91	97.0
92	97.0
93	97.0
94	55.0
95	13.0
96	13.0
97	13.0
98	11.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3311459036719476
2	0.011107619410379794
3	0.01022826620705806
4	0.011339028148096038
5	0.012634917079307013
6	0.08117818519085898
7	0.08066908596788325
8	0.10394880498213756
9	0.08483444324677566
10	0.0912676061552873
11	0.08737993936165438
12	0.09686769760802044
13	0.08409393528608368
14	0.08858326479777884
15	0.08469559800414592
16	0.08765762984691386
17	0.09876524925729366
18	0.1010793366344561
19	0.09520155469646346
20	0.09307259430947401
21	0.08187241140400772
22	0.10089420964428313
23	0.09446104673577148
24	0.0907122251847683
25	0.08923120926338433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2160679.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.054613159556915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9665116015369	39.773192729409764
2	13.79177633594181	14.634347165730482
3	4.92300281712463	7.835640301378685
4	2.1749488852029835	4.615642869850155
5	1.2188831230499309	3.233368629006335
6	0.7241987907674708	2.305325201687224
7	0.45745561742635643	1.6989091574155124
8	0.3076933145513369	1.3059639820242437
9	0.22963310495504174	1.0964785996815907
>10	1.0854878259263545	9.801750819922447
>50	0.06599239516384452	2.433062808607054
>100	0.044925686950717796	4.645003811837321
>500	0.005448250805192021	1.9905155395760221
>1k	0.0036028755324656546	3.1656633248097075
>5k	4.393750649348359E-4	1.4651350590634875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7995	0.37002257160827684	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6452	0.29860983514904343	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6341	0.29347256117174275	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5497	0.2544107662452405	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5141	0.23793446411984379	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4380	0.2027140542394312	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3309	0.1531463026206114	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3119	0.14435277058739404	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3032	0.1403262585511314	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2672	0.12366482943556169	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2621	0.12130446031085598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	3.2397223280274394E-4	0.0	0.0	0.0	0.0
11	3.2397223280274394E-4	0.0	0.0	0.0	0.0
12	3.2397223280274394E-4	0.0	0.0	0.0	0.0
13	3.2397223280274394E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	225	0.0	13.931379	19
TCCAACG	335	0.0	13.893897	18
TAGGACC	1245	0.0	12.588596	4
TAAGACG	70	1.0927087E-4	12.212574	4
GGTATCA	1885	0.0	12.075002	1
TAGACAG	180	0.0	11.609216	5
ACTGTTC	825	0.0	11.513537	8
CGCCCTA	400	0.0	11.398666	16
CCAACGT	135	4.7421054E-9	11.257682	19
TGTAGGA	3675	0.0	11.088242	2
TGTAGAA	1020	0.0	11.08178	2
GATATAC	740	0.0	11.067956	1
AGGACCT	2105	0.0	10.964954	5
GTATTAA	200	0.0	10.952127	1
GTCCTAC	3550	0.0	10.918593	1
CTGTAGG	3595	0.0	10.914378	1
CCGTTTC	270	0.0	10.905879	13
GGACCTG	2065	0.0	10.809622	6
GTATAGG	230	0.0	10.765797	1
CCCGTTT	265	0.0	10.753209	12
>>END_MODULE