FastQCFastQC Report
Thu 26 May 2016
SRR1512863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160679
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74930.3467891343415658No Hit
GTATCAACGCAGAGTACTTTTTTTT66930.30976373630696646No Hit
CTGTAGGACGTGGAATATGGCAAGA62920.29120475554212355No Hit
GTCCTAAAGTGTGTATTTCTCATTT58230.2694986159443397No Hit
CTTTAGGACGTGAAATATGGCGAGG55010.2545958932354135No Hit
TATCAACGCAGAGTACTTTTTTTTT50210.23238065441465394No Hit
GTCCTACAGTGTGCATTTCTCATTT41120.19031054589784047No Hit
GGTATCAACGCAGAGTACTTTTTTT39650.18350712900898283No Hit
CTGTAGGACCTGGAATATGGCGAGA29310.13565180204926322No Hit
CTGAAGGACCTGGAATATGGCGAGA28580.13227323447860603No Hit
GTACTGGTTCACTATCGGTCAGTCA24960.11551924186794985No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24960.11551924186794985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC402.7387144E-416.6432573
TAGGACC12050.012.5472914
CCAACGA2150.012.36303619
TCCAACG3450.012.10707318
CCGTACT550.003047157312.1025034
AATCGCC550.003085988312.08205918
GGCGAGG13750.011.73685719
TAGAAAT8350.011.5020374
GGTATCA18100.011.4564131
CTAGGAC2650.011.1254123
CCGAGCA951.36483195E-510.9970379
TTAGGAC19450.010.9528893
CGGTTTC2800.010.84944613
GACGTGA18050.010.7426567
AAATGTC7700.010.7395787
TGTAGGA38300.010.7023412
TGGCGAG34850.010.67797518
GTATCAA38300.010.6544071
GTATTGG1701.364242E-1010.6310321
GTCCTAT2700.010.5688621
Produced by FastQC (version 0.11.5)