##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512863_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2160679
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.16457141481914	31.0	30.0	34.0	26.0	34.0
2	30.196963084289706	31.0	30.0	34.0	26.0	34.0
3	30.374097216661983	31.0	30.0	34.0	26.0	34.0
4	33.95225528641691	37.0	35.0	37.0	30.0	37.0
5	33.518922523891796	37.0	35.0	37.0	28.0	37.0
6	33.38564636394393	37.0	35.0	37.0	27.0	37.0
7	33.21465937328034	37.0	35.0	37.0	26.0	37.0
8	33.162371643358405	37.0	35.0	37.0	26.0	37.0
9	34.4824886065908	39.0	35.0	39.0	26.0	39.0
10	34.40274608120873	39.0	35.0	39.0	26.0	39.0
11	34.38562692560996	39.0	35.0	39.0	26.0	39.0
12	34.24300185265835	39.0	35.0	39.0	25.0	39.0
13	34.23359092211291	39.0	35.0	39.0	25.0	39.0
14	35.30376330773798	40.0	35.0	41.0	25.0	41.0
15	35.30654437794786	40.0	36.0	41.0	25.0	41.0
16	35.306756348351605	40.0	36.0	41.0	25.0	41.0
17	35.291659242303	40.0	36.0	41.0	25.0	41.0
18	35.22304886565751	40.0	36.0	41.0	25.0	41.0
19	35.23325121408594	40.0	35.0	41.0	25.0	41.0
20	35.206336526619644	40.0	35.0	41.0	25.0	41.0
21	35.17049362723477	40.0	35.0	41.0	25.0	41.0
22	35.13401851917846	40.0	35.0	41.0	24.0	41.0
23	34.97033987926943	40.0	35.0	41.0	23.0	41.0
24	34.92953742781783	40.0	35.0	41.0	23.0	41.0
25	34.86718943443242	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7571954681530819
1101	2	0.6813189334895497
1101	3	0.6502314510457659
1101	4	0.6528170123828758
1101	5	0.7472502127244454
1101	6	0.756303268207013
1101	7	0.7524210702182188
1101	8	0.5369122952898095
1101	9	0.8836824906098286
1101	10	0.5921414489962018
1101	11	0.9388261589338001
1101	12	1.0444774591769814
1101	13	1.0586664178316525
1101	14	1.2492400093911087
1101	15	0.7978509899320301
1101	16	1.1825494574154192
1101	17	1.2031658609593805
1101	18	1.0668933478344798
1101	19	1.0971526839695542
1101	20	1.061576130143827
1101	21	1.0448728572814545
1101	22	1.03199586664919
1101	23	1.1329196285194598
1101	24	1.1222920216393177
1101	25	1.2963788077701395
1102	1	0.7489687377651926
1102	2	0.8433251236961219
1102	3	0.590818946096153
1102	4	0.6632994811577078
1102	5	0.8475689746675457
1102	6	0.8742225937389918
1102	7	1.009775373358373
1102	8	1.0201972572871014
1102	9	1.194899888163775
1102	10	1.002094900715143
1102	11	1.1767542140944585
1102	12	1.0391030502334146
1102	13	1.1861055573029518
1102	14	1.221656174668965
1102	15	1.3518364978135295
1102	16	1.3633613557317972
1102	17	1.4777949734724487
1102	18	0.8500049248374211
1102	19	1.1034067753743386
1102	20	1.3058605313958935
1102	21	1.2364430198703005
1102	22	1.0289539358002386
1102	23	1.385268710827468
1102	24	1.2358379629170813
1102	25	1.3615602299828566
1103	1	0.7439853588269827
1103	2	0.8517457473159347
1103	3	0.6513148507605919
1103	4	0.5663380403521288
1103	5	0.7949865653797801
1103	6	0.7264070382154699
1103	7	0.8346498998932788
1103	8	0.8573209980956378
1103	9	0.8263047054648567
1103	10	0.6787326556352724
1103	11	0.9261173641915192
1103	12	0.9718491970828893
1103	13	1.0634021427405997
1103	14	1.1202947230911775
1103	15	1.0879504010168901
1103	16	0.9974248981041285
1103	17	1.0848329804015577
1103	18	1.0131143606088884
1103	19	1.0283336836671921
1103	20	0.976268043266181
1103	21	1.0970604153172943
1103	22	1.0970510745652433
1103	23	1.1081031177150038
1103	24	1.0589076704819789
1103	25	1.1159358705309685
1104	1	0.7517369288195148
1104	2	0.8788156678482366
1104	3	0.7998634330481984
1104	4	0.6857445805638562
1104	5	0.8838846645267537
1104	6	0.7136960115966602
1104	7	0.7121750940112861
1104	8	0.7714799730361932
1104	9	0.852934890058819
1104	10	0.8302339505013947
1104	11	0.9774708742076115
1104	12	0.9470393234209311
1104	13	0.8089984414603535
1104	14	1.0902931674611764
1104	15	1.1898135757107866
1104	16	1.1772616949836205
1104	17	1.0582557692992083
1104	18	0.7188339308819565
1104	19	1.133757367145357
1104	20	1.0155873376294267
1104	21	1.0340289550321202
1104	22	0.98158574347719
1104	23	0.9657035640353229
1104	24	1.225758687386488
1104	25	1.042036544040414
1105	1	0.5328226402704495
1105	2	0.6125078369517958
1105	3	0.6127551421644171
1105	4	0.4530989522284301
1105	5	0.5487827499081561
1105	6	0.656488014685209
1105	7	0.7028744823606488
1105	8	0.7038318304035016
1105	9	0.8843467645347047
1105	10	0.6971564158709995
1105	11	0.9539336835682306
1105	12	0.9799043097767282
1105	13	0.9632691131118065
1105	14	1.0241017978620448
1105	15	1.0458957312662491
1105	16	1.0298880876178131
1105	17	0.8694881520567179
1105	18	0.8631964224361326
1105	19	1.0562491885857668
1105	20	1.1402547447033982
1105	21	1.1131759574900357
1105	22	0.866064081254855
1105	23	1.053220879003824
1105	24	1.0460575510193166
1105	25	0.888256100861426
1106	1	0.17346760960682417
1106	2	0.5042560179278439
1106	3	-1.487346915346162
1106	4	-0.3172252760613503
1106	5	0.16721138329094032
1106	6	0.3309124989905783
1106	7	0.3957691130820038
1106	8	0.4028645656004315
1106	9	0.7051451280530898
1106	10	0.44466646859143566
1106	11	0.49941067351347357
1106	12	0.6446676465342378
1106	13	0.6981501517463826
1106	14	0.6597488799484097
1106	15	0.8410973588442587
1106	16	0.7521338054501285
1106	17	0.8157886878679221
1106	18	0.7964517008795156
1106	19	0.7050813887505569
1106	20	0.8407532349867921
1106	21	0.762122606008198
1106	22	0.8507576805683925
1106	23	0.9036780602948369
1106	24	0.9369973593470746
1106	25	0.8927687419324712
1107	1	0.6574062899740731
1107	2	0.816390312204998
1107	3	0.6677479897487437
1107	4	0.4895453342806917
1107	5	0.6062366437447722
1107	6	0.6701922668417737
1107	7	0.7119231970932276
1107	8	0.6182087452303264
1107	9	0.6280377116599638
1107	10	0.7008864390971254
1107	11	0.693347688879058
1107	12	0.9844766470575266
1107	13	0.7624727339261028
1107	14	1.0045945105083263
1107	15	0.9541450254633546
1107	16	1.080585116044297
1107	17	1.0280169358104558
1107	18	0.9267440276004137
1107	19	1.0618852991258976
1107	20	0.9908888136938003
1107	21	0.938047871219176
1107	22	0.9886138719960087
1107	23	1.0126524192691946
1107	24	1.106012867648296
1107	25	1.2113965427031275
1108	1	0.5191695267373113
1108	2	0.4081886653669855
1108	3	0.45066961357946056
1108	4	0.3069665494291769
1108	5	0.5013036979027348
1108	6	0.40197563498878
1108	7	0.4148982523410112
1108	8	0.35319345748776954
1108	9	0.5877560236510035
1108	10	0.4738029165590518
1108	11	0.5504451368855996
1108	12	0.5854249411876395
1108	13	0.7015374570817343
1108	14	0.6634735102964129
1108	15	0.6543879643426536
1108	16	0.6423907346680977
1108	17	0.6369080447015634
1108	18	0.6903629844011903
1108	19	0.6793322461182996
1108	20	0.7582351037673618
1108	21	0.7384255238695587
1108	22	0.7703056650514384
1108	23	0.7961416573038917
1108	24	0.7810688722497545
1108	25	0.7588226014928239
1109	1	0.16922197283856732
1109	2	0.16524847999111358
1109	3	0.276819191643888
1109	4	0.219748237130311
1109	5	0.04363514491433307
1109	6	0.2748564386180874
1109	7	0.15135590751664552
1109	8	0.2718371718105246
1109	9	0.4399252479083344
1109	10	0.05583064267584348
1109	11	0.31708348242891304
1109	12	0.541498673065604
1109	13	0.3541773928155365
1109	14	0.5616912003766572
1109	15	0.42042895942596203
1109	16	0.461996726900999
1109	17	0.5031421882454126
1109	18	0.6115638320454337
1109	19	0.5330100246811895
1109	20	0.6737919071140297
1109	21	0.7083796361035297
1109	22	0.5837110806173342
1109	23	0.5195286052563688
1109	24	0.636829402459604
1109	25	0.5547170562008645
1110	1	0.4289162522735097
1110	2	0.2744527452674319
1110	3	0.2942662083722283
1110	4	0.3667785207244023
1110	5	0.3822715855691854
1110	6	0.38962579680161724
1110	7	0.3387730778904796
1110	8	0.3840264031532499
1110	9	0.3232554266145229
1110	10	0.32090321700863456
1110	11	0.4509176438174336
1110	12	0.5208463347430055
1110	13	0.5090086423041171
1110	14	0.561115845977028
1110	15	0.5142244552796242
1110	16	0.4300291404883225
1110	17	0.5500548552167928
1110	18	0.5707392097534907
1110	19	0.5486203871767756
1110	20	0.5944977225658619
1110	21	0.7296268351665631
1110	22	0.7121114862830993
1110	23	0.785125536479157
1110	24	0.7672604512643062
1110	25	0.7332418974432215
1111	1	0.116330931413831
1111	2	0.037540970261787265
1111	3	-0.013612616244742526
1111	4	0.11723592040058861
1111	5	0.35629570743758165
1111	6	0.24028778222954372
1111	7	0.09751556566141062
1111	8	0.17402074683847246
1111	9	0.23814980179652423
1111	10	0.2579678067169269
1111	11	0.17664289928339372
1111	12	0.28070648737748627
1111	13	0.38572481776701295
1111	14	0.18160571314578533
1111	15	0.3104629445152156
1111	16	0.2371808358058658
1111	17	0.3701311274063599
1111	18	0.23149900724021677
1111	19	0.03715346842660239
1111	20	0.23591185860166775
1111	21	0.29070434470058615
1111	22	0.1012343233365911
1111	23	0.1395277754131996
1111	24	0.35714707512428845
1111	25	0.25565446109724377
1112	1	0.09402404025353661
1112	2	-0.23350851882766221
1112	3	0.057868092694125295
1112	4	0.11060039446214631
1112	5	-0.006169227954778478
1112	6	-0.11729657640915292
1112	7	0.014876594377483343
1112	8	0.05361875034293462
1112	9	0.12170652735480303
1112	10	-0.2237156007465515
1112	11	-0.15113589445211062
1112	12	0.09178399274777149
1112	13	0.021754499248764603
1112	14	-0.23491814536120614
1112	15	-0.3632211585385221
1112	16	-0.3709956761498745
1112	17	-0.34845510515972933
1112	18	-0.1447534030977593
1112	19	-0.2094977478147726
1112	20	-0.18723789231395216
1112	21	-0.18888018832969777
1112	22	0.022388492666543414
1112	23	0.08953000265236
1112	24	0.14203183628808347
1112	25	0.07373850681089067
1113	1	-0.6669450539311512
1113	2	-0.6956202245737906
1113	3	-0.5316013283723002
1113	4	-1.9899689231883748
1113	5	-0.809449543378264
1113	6	-0.5165087301141469
1113	7	-0.576009949327748
1113	8	-0.4607083785883006
1113	9	-0.5231691830289691
1113	10	-0.5672375767093953
1113	11	-0.6412997031263856
1113	12	-0.3545906684002915
1113	13	-0.5326330390186058
1113	14	-0.6512416386650202
1113	15	-0.8193490065764522
1113	16	-0.929658936928611
1113	17	-1.0676486963557608
1113	18	-0.8808169417508225
1113	19	-0.6741452307922344
1113	20	-0.38219783314719535
1113	21	-0.6175825258075136
1113	22	-0.5462502113906709
1113	23	-0.3267636686984545
1113	24	-0.42204593141281777
1113	25	-0.6456664888964028
1114	1	-0.9513589009936219
1114	2	-2.1633834804602365
1114	3	-1.5053633456799176
1114	4	-1.089736292234754
1114	5	-0.9575428266108972
1114	6	-0.589905325197364
1114	7	-0.670768832519471
1114	8	-0.7847538106549834
1114	9	-1.0350352105465745
1114	10	-1.2391290082252127
1114	11	-1.182971711970616
1114	12	-1.0872297432814335
1114	13	-1.2826256089279795
1114	14	-1.1989729728086402
1114	15	-1.4081870579021327
1114	16	-1.6760412603628723
1114	17	-1.698619162955083
1114	18	-1.4422935979612248
1114	19	-2.0473390707978467
1114	20	-1.2953218441243735
1114	21	-1.6050500364403177
1114	22	-1.179821641690026
1114	23	-1.0569496905208027
1114	24	-1.206739011648466
1114	25	-1.7441068091989678
1115	1	-1.1608545424283143
1115	2	-1.3316993989176886
1115	3	-0.7796543804790943
1115	4	-0.5806416561024363
1115	5	-0.9272140348167639
1115	6	-0.9171911664768544
1115	7	-0.8774640832406106
1115	8	-1.0778157627612046
1115	9	-1.9998061376875853
1115	10	-1.7107183480051091
1115	11	-1.94436465562692
1115	12	-2.5355573505915956
1115	13	-2.5695431987862456
1115	14	-2.138063097186233
1115	15	-2.4111864168294304
1115	16	-2.019744225828532
1115	17	-1.9506989191134707
1115	18	-2.529456087425743
1115	19	-2.9486726240954724
1115	20	-3.041245623784583
1115	21	-2.2345090819659745
1115	22	-2.1755158915510364
1115	23	-2.2647371894166
1115	24	-2.8496419333021237
1115	25	-2.772429726725754
1116	1	-1.8958967182013744
1116	2	-1.9568508217228775
1116	3	-1.9954026389165662
1116	4	-1.7494597647436905
1116	5	-2.2688765007988074
1116	6	-2.9994754529594374
1116	7	-2.5770762317385945
1116	8	-2.8997126935602893
1116	9	-4.10229545018462
1116	10	-2.602044289819961
1116	11	-3.7306456272085917
1116	12	-5.8058959018807315
1116	13	-4.653976200803758
1116	14	-3.9894172129605323
1116	15	-5.139414966902635
1116	16	-4.52768971749752
1116	17	-4.356416414879199
1116	18	-4.36210604006158
1116	19	-4.641564407994828
1116	20	-6.253569783182478
1116	21	-5.371278679319179
1116	22	-4.566645698355522
1116	23	-5.39493398720478
1116	24	-5.260741842587574
1116	25	-4.4525388371548225
1201	1	0.725051402513003
1201	2	0.8452142625623651
1201	3	0.8926915346485664
1201	4	0.7930297168562674
1201	5	0.23269206423517375
1201	6	0.6767994427181563
1201	7	0.9139438237703175
1201	8	0.9307796325982167
1201	9	0.9078975821663064
1201	10	0.9399517668570141
1201	11	0.7315526132488799
1201	12	1.049371802911459
1201	13	1.1560108271050495
1201	14	1.1176124523832343
1201	15	0.9748090116957329
1201	16	0.960712665987586
1201	17	0.9503262005619462
1201	18	1.1305402053972173
1201	19	1.2992182087729844
1201	20	1.2704933440790924
1201	21	0.842143905312092
1201	22	0.9181998752078755
1201	23	1.0272182719050065
1201	24	0.7847693339594173
1201	25	0.9730106053009564
1202	1	0.8772223528294738
1202	2	0.8692782407075548
1202	3	0.8417704945948508
1202	4	0.8428399345789757
1202	5	0.8205094125657908
1202	6	0.8365344270376482
1202	7	0.9604211905973656
1202	8	0.8201617297586026
1202	9	1.1398383214169314
1202	10	1.0739105671919305
1202	11	0.9052729793230938
1202	12	1.010167279923941
1202	13	0.9524775406334314
1202	14	1.0623151140187232
1202	15	1.123875341244684
1202	16	1.11329132092321
1202	17	1.0650224932523997
1202	18	1.0939026475094948
1202	19	1.2343864560308617
1202	20	1.0509891313233126
1202	21	1.242295431689847
1202	22	1.0561683468184597
1202	23	1.1917896618667996
1202	24	1.2263401177389142
1202	25	1.264554535089765
1203	1	0.8928322471915564
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2211	10	0.11193447172703941
2211	11	0.16274325949486013
2211	12	0.07047005431673625
2211	13	0.16649888762147214
2211	14	0.07009492208342039
2211	15	0.20244175225904826
2211	16	0.17201758585812854
2211	17	-0.05130722429876755
2211	18	-0.08891733005594915
2211	19	0.004991724700715849
2211	20	0.010173557177303394
2211	21	0.07615607537413638
2211	22	0.10393106240140781
2211	23	-0.12494542834041056
2211	24	0.14978864249581392
2211	25	0.0014587176153497694
2212	1	-0.3664555294997527
2212	2	-0.26956136121780716
2212	3	-0.23751937568028225
2212	4	-0.263010424048737
2212	5	-0.5192592998805026
2212	6	-0.3342496922768632
2212	7	-0.6117812931816999
2212	8	-0.38735564803614153
2212	9	-0.5399027966385148
2212	10	-0.585805987029481
2212	11	-0.2620577601753524
2212	12	-0.1531493912613513
2212	13	-0.2557513437667964
2212	14	-0.21801352041379118
2212	15	-0.4011558514887952
2212	16	-0.4405984732686221
2212	17	-0.417589420881356
2212	18	-0.5596649637162017
2212	19	-0.3560834719029202
2212	20	-0.5434160846411231
2212	21	-0.3081581936935862
2212	22	-0.36830336396279506
2212	23	-0.7243749875041701
2212	24	-0.6968088295151986
2212	25	-0.5276711640320144
2213	1	-0.525477303541539
2213	2	-0.19050394767659284
2213	3	-0.27333043945234436
2213	4	-0.4931873447880122
2213	5	-0.2480809121996188
2213	6	-0.25668889499475966
2213	7	-0.8037225569991122
2213	8	-0.789411444350705
2213	9	-0.49723747145426245
2213	10	-0.5422049545845411
2213	11	-0.42341468781709324
2213	12	-0.6104538926973717
2213	13	-0.5572639527483858
2213	14	-0.6991889888409659
2213	15	-0.9953245590839757
2213	16	-1.4360123689162734
2213	17	-1.1539114870479992
2213	18	-0.7644698957255187
2213	19	-0.8188787803496709
2213	20	-0.7367342686658205
2213	21	-0.5944093898228786
2213	22	-0.7346424402683667
2213	23	-0.8443478364497423
2213	24	-0.6232733107092869
2213	25	-0.6706340502762131
2214	1	-1.2956356680678844
2214	2	-0.4967024098877246
2214	3	-0.6201665401657834
2214	4	-0.7150599493245906
2214	5	-0.9263844388763118
2214	6	-1.4618812892317834
2214	7	-0.9814119492419167
2214	8	-0.799034974013388
2214	9	-0.5187908301685766
2214	10	-0.8833186891080089
2214	11	-1.0200514305200628
2214	12	-0.5750605194796421
2214	13	-1.1726355281821554
2214	14	-0.6701322442184292
2214	15	-0.7891471268288015
2214	16	-0.8519087974496173
2214	17	-0.9237971020035829
2214	18	-0.9717607639043777
2214	19	-0.9744656462250489
2214	20	-1.088236801431819
2214	21	-1.2208697666984563
2214	22	-1.6233713649892252
2214	23	-1.5420458454290653
2214	24	-1.2681210354856063
2214	25	-1.7754604891539003
2215	1	-1.6291672665808932
2215	2	-1.0021673692709
2215	3	-1.1234301629535715
2215	4	-1.1790764714229027
2215	5	-1.494301913113489
2215	6	-1.5573880834151588
2215	7	-1.8979057905238612
2215	8	-1.7330555419075644
2215	9	-1.6315105158250702
2215	10	-1.7138500016616263
2215	11	-1.3959171355158801
2215	12	-1.6042411716788791
2215	13	-1.7243135590869514
2215	14	-2.003576995804444
2215	15	-1.7332526140718372
2215	16	-1.76676347712214
2215	17	-1.9338139841989062
2215	18	-1.6712046470211277
2215	19	-2.046786852761869
2215	20	-2.0002204487909268
2215	21	-1.657567634712315
2215	22	-1.7722926626094093
2215	23	-2.252477432291883
2215	24	-2.119335049614115
2215	25	-2.3898016704839335
2216	1	-1.9988849677877703
2216	2	-1.346684358222614
2216	3	-1.3991880154225562
2216	4	-1.8076687261686644
2216	5	-2.5417594777807615
2216	6	-2.801878799346941
2216	7	-2.7681109844115426
2216	8	-2.9533069939324683
2216	9	-4.859207885879751
2216	10	-2.8620525826654273
2216	11	-3.0708537959106614
2216	12	-3.405908947739835
2216	13	-3.294912527870924
2216	14	-3.5710161533376343
2216	15	-3.4346784060071407
2216	16	-2.8249333850624367
2216	17	-3.1618823805594474
2216	18	-3.6019767614144946
2216	19	-3.7587768818304
2216	20	-3.085519092125871
2216	21	-3.6515737816377687
2216	22	-3.0699633351106073
2216	23	-4.130648397561032
2216	24	-3.5127061584236685
2216	25	-3.7047975573144996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64622.0
3	1.0
4	1151.0
5	9408.0
6	11115.0
7	8511.0
8	6878.0
9	5800.0
10	4978.0
11	4403.0
12	3965.0
13	3853.0
14	3636.0
15	3693.0
16	3739.0
17	3945.0
18	4402.0
19	5281.0
20	6933.0
21	9012.0
22	11287.0
23	13677.0
24	16202.0
25	18294.0
26	20294.0
27	22402.0
28	25272.0
29	30529.0
30	37614.0
31	48045.0
32	61158.0
33	78528.0
34	102282.0
35	133908.0
36	183920.0
37	283745.0
38	908196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.313444222464426	19.347774026300076	13.741556280117265	26.59722547111823
2	13.057628305209995	19.915250313459012	41.82010878926109	25.207012592069905
3	17.858817615000646	25.01181588786734	29.233131518122864	27.89623497900915
4	11.455227438300204	16.737975933816607	36.92105962300201	34.885737004881186
5	12.59858156422331	39.16525133235845	34.88207707658359	13.354090026834644
6	32.19149091845363	36.59352404380037	17.836062597884446	13.378922439861556
7	28.626420732845485	31.501860344259796	21.150305533696248	18.72141338919847
8	25.51012611628074	37.936758948189755	19.74512795697182	16.807986978557686
9	27.130585815043396	14.584224452875747	19.874511851658454	38.410677880422405
10	15.86482963635227	29.207392881700468	33.19772462603808	21.730052855909186
11	34.72620977801393	21.917936294659533	24.64026371172776	18.71559021559878
12	24.392122269311457	24.394714054371022	31.211849271037313	20.00131440528021
13	29.64155917731069	21.9148225152787	25.616331963912458	22.82728634349815
14	20.779869929114515	22.834789981616797	27.05677133182886	29.328568757439825
15	23.050524538321106	32.32580810007147	22.638512925527777	21.985154436079647
16	21.723075100105856	27.69401743978027	28.622383257662825	21.960524202451047
17	21.174664402427346	29.103575063560232	27.931049006525654	21.790711527486764
18	21.05812974507632	26.895079862385558	31.18092837448606	20.865862018052063
19	23.67307343406929	26.307024981579463	27.56138018875959	22.45852139559166
20	24.22642599251245	26.93449619166058	28.28084842869262	20.558229387134354
21	23.023036782486955	26.26989251044234	26.993046154558183	23.714024552512527
22	23.608880578709382	28.42858928017698	26.934932220098396	21.02759792101524
23	21.987069805371366	27.946261337292583	28.15304818531582	21.913620672020233
24	23.539683590204746	26.269658750790843	28.840424699828155	21.35023295917626
25	22.408927934228082	27.411244335692626	28.540472694000357	21.639355036078936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	71.0
2	231.5
3	392.0
4	392.0
5	392.0
6	1072.0
7	1752.0
8	1752.0
9	1752.0
10	2253.5
11	2755.0
12	2755.0
13	2755.0
14	4319.5
15	5884.0
16	5884.0
17	5884.0
18	12372.5
19	18861.0
20	18861.0
21	18861.0
22	33557.0
23	48253.0
24	48253.0
25	48253.0
26	74984.0
27	101715.0
28	101715.0
29	101715.0
30	135610.5
31	169506.0
32	169506.0
33	169506.0
34	189575.5
35	209645.0
36	209645.0
37	209645.0
38	223649.5
39	237654.0
40	237654.0
41	237654.0
42	251834.5
43	266015.0
44	266015.0
45	266015.0
46	280909.0
47	295803.0
48	295803.0
49	295803.0
50	283220.0
51	270637.0
52	270637.0
53	270637.0
54	235698.0
55	200759.0
56	200759.0
57	200759.0
58	172499.5
59	144240.0
60	144240.0
61	144240.0
62	120289.0
63	96338.0
64	96338.0
65	96338.0
66	73733.0
67	51128.0
68	51128.0
69	51128.0
70	37656.0
71	24184.0
72	24184.0
73	24184.0
74	17067.0
75	9950.0
76	9950.0
77	9950.0
78	6778.5
79	3607.0
80	3607.0
81	3607.0
82	2382.5
83	1158.0
84	1158.0
85	1158.0
86	732.0
87	306.0
88	306.0
89	306.0
90	183.5
91	61.0
92	61.0
93	61.0
94	32.5
95	4.0
96	4.0
97	4.0
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08451047101397292
2	0.041977545021726966
3	0.020919349889548612
4	0.0063405994134251315
5	0.019068079987818645
6	0.057620775691345175
7	0.07729051839722606
8	0.053871954140341995
9	0.02198383008304334
10	0.047855326959719606
11	0.006386881160968381
12	2.7769048525949484E-4
13	0.007173670869203616
14	5.090992229757405E-4
15	0.01402336950560449
16	0.009996857469341813
17	0.006016627180622388
18	0.006942262131487371
19	0.0021752421345327096
20	0.0012033254361244776
21	1.8512699017299655E-4
22	4.165357278892422E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2160679.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.83394695689494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57582853760243	38.137255937276805
2	14.58124544699675	15.116070061301906
3	5.194265055565932	8.077177781107723
4	2.356911616799897	4.886721269091813
5	1.255746299776641	3.2545143546969744
6	0.7463946218587564	2.3213147543003054
7	0.49204994318637574	1.7853423458686037
8	0.32943808928668006	1.3660861156533275
9	0.24514748969336955	1.143626577964386
>10	1.0969435929263878	9.830511481647529
>50	0.06964275816188771	2.4821484934475113
>100	0.04670848688393818	4.742482515508697
>500	0.005197477343833664	1.8211365217917548
>1k	0.003942913847046205	3.3252103277993106
>5k	5.376700700517553E-4	1.7104014625433595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7493	0.3467891343415658	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6693	0.30976373630696646	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6292	0.29120475554212355	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5823	0.2694986159443397	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5501	0.2545958932354135	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5021	0.23238065441465394	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4112	0.19031054589784047	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3965	0.18350712900898283	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2931	0.13565180204926322	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2858	0.13227323447860603	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2496	0.11551924186794985	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2496	0.11551924186794985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.628174754324914E-5
10	3.2397223280274394E-4	0.0	0.0	0.0	4.628174754324914E-5
11	3.2397223280274394E-4	0.0	0.0	0.0	4.628174754324914E-5
12	3.2397223280274394E-4	0.0	0.0	0.0	4.628174754324914E-5
13	3.2397223280274394E-4	0.0	0.0	0.0	4.628174754324914E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	40	2.7387144E-4	16.643257	3
TAGGACC	1205	0.0	12.547291	4
CCAACGA	215	0.0	12.363036	19
TCCAACG	345	0.0	12.107073	18
CCGTACT	55	0.0030471573	12.102503	4
AATCGCC	55	0.0030859883	12.082059	18
GGCGAGG	1375	0.0	11.736857	19
TAGAAAT	835	0.0	11.502037	4
GGTATCA	1810	0.0	11.456413	1
CTAGGAC	265	0.0	11.125412	3
CCGAGCA	95	1.36483195E-5	10.997037	9
TTAGGAC	1945	0.0	10.952889	3
CGGTTTC	280	0.0	10.849446	13
GACGTGA	1805	0.0	10.742656	7
AAATGTC	770	0.0	10.739578	7
TGTAGGA	3830	0.0	10.702341	2
TGGCGAG	3485	0.0	10.677975	18
GTATCAA	3830	0.0	10.654407	1
GTATTGG	170	1.364242E-10	10.631032	1
GTCCTAT	270	0.0	10.568862	1
>>END_MODULE