FastQCFastQC Report
Thu 26 May 2016
SRR1512862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177067
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43520.19990197821197053No Hit
TATCAACGCAGAGTACTTTTTTTTT26140.12006980033228193No Hit
GTACTGGTTCACTATCGGTCAGTCA24220.11125059541116558No Hit
GGTATCAACGCAGAGTACTTTTTTT24150.11092906189841653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG400.005284157614.248314
GCACCGT1155.336915E-912.3898356
GTCCTAC8400.011.6756171
GGTATCA15050.011.6413471
GTATCAA34450.011.4428191
GCCTACG1001.8778974E-611.4262351
ATACCGT600.00588212811.0820196
CCGTCCG600.00588313511.0817649
GTCCTAA5350.011.0346811
ATTAGAC700.001493293610.8558563
ACCGTCC700.001493293610.8558568
CGAAATC1158.836869E-710.7376113
GTACTGG8400.010.2019961
TAGGACC3100.010.1117044
TGGACCG1606.186383E-910.0925535
GTAGGAC7050.09.97044853
TACCGTG1151.0281914E-59.9118687
TCCAACG1252.5746613E-69.87882818
TAGAGTG2800.09.83811955
ATTTCTC10650.09.81081815
Produced by FastQC (version 0.11.5)