##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512862_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2177067
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.09373023430147	31.0	28.0	33.0	16.0	34.0
2	28.22541290644707	31.0	28.0	34.0	16.0	34.0
3	28.326259596052854	31.0	28.0	34.0	16.0	34.0
4	31.691638337267527	35.0	32.0	37.0	17.0	37.0
5	31.860508656830497	35.0	32.0	37.0	18.0	37.0
6	31.699197590152256	35.0	32.0	37.0	17.0	37.0
7	31.657618713617907	35.0	32.0	37.0	17.0	37.0
8	31.564791069820085	35.0	32.0	37.0	16.0	37.0
9	32.77194914074762	37.0	32.0	39.0	13.0	39.0
10	32.64166605804966	37.0	32.0	39.0	11.0	39.0
11	32.715891150800594	37.0	32.0	39.0	11.0	39.0
12	32.593515036514724	37.0	32.0	39.0	10.0	39.0
13	32.56113385577936	37.0	32.0	39.0	10.0	39.0
14	33.350926728483785	38.0	32.0	41.0	10.0	41.0
15	33.50508321516977	38.0	33.0	41.0	10.0	41.0
16	33.424728774998655	38.0	33.0	41.0	8.0	41.0
17	33.153290642869514	38.0	32.0	41.0	7.0	41.0
18	33.125486721354925	38.0	32.0	41.0	2.0	41.0
19	33.239506638978035	39.0	32.0	41.0	2.0	41.0
20	33.20393492712902	39.0	32.0	41.0	2.0	41.0
21	33.10084714893937	39.0	32.0	41.0	2.0	41.0
22	33.07283698664304	39.0	32.0	41.0	2.0	41.0
23	32.71491139225389	38.0	32.0	41.0	2.0	41.0
24	32.76366000678895	39.0	32.0	41.0	2.0	41.0
25	32.453982812655745	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7480417537309805
1101	2	0.4230312464621875
1101	3	0.7050588918317935
1101	4	0.9144928256367635
1101	5	0.5896324501466559
1101	6	0.6147532824284703
1101	7	0.34205591158330506
1101	8	0.7878105612048891
1101	9	0.8462794730883161
1101	10	0.7985673943866303
1101	11	0.7338540296715266
1101	12	0.5819232262229335
1101	13	1.023176219585494
1101	14	1.1480514491163873
1101	15	0.8828406520681398
1101	16	0.7563105896958504
1101	17	0.07337952937370318
1101	18	0.8066049900885588
1101	19	-0.020535639834207586
1101	20	-0.6866356146567156
1101	21	0.5288601969155096
1101	22	0.5892007074064622
1101	23	0.2584939445047496
1101	24	0.7817434637613658
1101	25	0.5609879930960986
1102	1	0.9966588765027602
1102	2	0.9989505702549728
1102	3	0.8572446814376207
1102	4	1.0097148525846222
1102	5	0.9249719764668463
1102	6	0.7949372308760339
1102	7	0.8485073301120565
1102	8	0.9662326885795629
1102	9	1.34178721432189
1102	10	1.236652589800478
1102	11	0.9545206963381929
1102	12	1.1495424412200066
1102	13	1.0130625699076958
1102	14	1.2519163750516142
1102	15	1.130550251197775
1102	16	1.0637570705729118
1102	17	1.392120679260728
1102	18	1.3187189745223975
1102	19	1.081658518672782
1102	20	1.189061598499194
1102	21	1.4468667246584133
1102	22	1.1402024648757063
1102	23	1.0960537403035602
1102	24	0.558195551802207
1102	25	0.887982051176273
1103	1	0.409295482364616
1103	2	0.6049436016138579
1103	3	0.7634492315503465
1103	4	0.886294479557364
1103	5	0.6134917551100152
1103	6	0.37735616918306647
1103	7	0.12390228809524828
1103	8	0.3295272161466869
1103	9	0.586230337912788
1103	10	0.6875072731372285
1103	11	0.45475312101537924
1103	12	0.647180891722158
1103	13	0.7710883948718248
1103	14	0.8426476724486207
1103	15	0.756534674235283
1103	16	0.8644666422064446
1103	17	0.4664600930750282
1103	18	0.9849518091196501
1103	19	0.8306279796278986
1103	20	1.0394354455990111
1103	21	0.7908388500939552
1103	22	0.8145783615758546
1103	23	0.10065673272004005
1103	24	0.35757124354231706
1103	25	0.7370716187106012
1104	1	1.1485659059812185
1104	2	1.1546988695747018
1104	3	1.1247911666983903
1104	4	1.3757647392059411
1104	5	1.0883551964214995
1104	6	0.8718374576334078
1104	7	1.1677431577764494
1104	8	0.9411896046793338
1104	9	1.3208733821223433
1104	10	1.4129677825442428
1104	11	1.0382757983790043
1104	12	1.2968485636689806
1104	13	1.4414798034677858
1104	14	1.6559685292466213
1104	15	1.2994550131025377
1104	16	1.457686775788332
1104	17	1.7216739672425874
1104	18	1.4218935776970056
1104	19	1.0136607862464828
1104	20	1.1662067232157511
1104	21	1.2933179718239458
1104	22	1.0335584739460018
1104	23	1.5728836722763546
1104	24	1.4637306991944996
1104	25	0.8704809173894219
1105	1	0.5271953656546344
1105	2	0.5847043037010167
1105	3	0.7206800153344908
1105	4	0.964065937397244
1105	5	0.7239936727193133
1105	6	0.8837740863282768
1105	7	0.811091720081059
1105	8	0.7941092735330706
1105	9	0.5303975270860519
1105	10	0.6658777236409961
1105	11	0.6713011978879209
1105	12	0.909113588021917
1105	13	0.764273615272586
1105	14	0.8284500465083582
1105	15	0.8263287803609174
1105	16	0.8034364168676476
1105	17	1.0943359511683042
1105	18	0.8245656919075799
1105	19	0.7361194935952966
1105	20	0.8544304347911122
1105	21	0.6903832081749002
1105	22	0.6530455192572262
1105	23	0.9256389755811085
1105	24	0.9951563858399695
1105	25	1.1835436178147631
1106	1	0.7372679470607579
1106	2	0.8120058724687205
1106	3	0.6695030348079847
1106	4	0.7642035351150938
1106	5	0.6747598879169736
1106	6	0.7074816301795757
1106	7	0.7839992462052479
1106	8	0.9171593115793151
1106	9	1.0073656904018193
1106	10	0.8011978182226613
1106	11	0.5963999766632071
1106	12	0.9314263646094432
1106	13	0.9188060405983478
1106	14	1.2684906226264374
1106	15	0.9799990531213254
1106	16	1.03436133224276
1106	17	1.065325057007179
1106	18	1.131068696767656
1106	19	1.1955212166248614
1106	20	1.3172653958625986
1106	21	1.2384146891721386
1106	22	1.2438937010916078
1106	23	1.4536129808308829
1106	24	1.1096080383290285
1106	25	1.33751127760857
1107	1	0.8092927753453161
1107	2	0.792215196860333
1107	3	0.7624097361387463
1107	4	0.9507337205091488
1107	5	0.783257056536609
1107	6	0.9159893951379665
1107	7	0.5316714334892509
1107	8	0.7358873579944962
1107	9	0.6183694245644773
1107	10	0.8002343243811012
1107	11	0.708789286793241
1107	12	0.7807100327849312
1107	13	0.5536863212950394
1107	14	0.7226590419296812
1107	15	0.6443165426200039
1107	16	0.7133283405903583
1107	17	0.9880959448386548
1107	18	0.8568908470449941
1107	19	0.691286868125772
1107	20	0.8509280960416135
1107	21	0.7096810579388944
1107	22	0.6109793794151059
1107	23	0.9663033042800961
1107	24	0.5802317021113907
1107	25	0.9014851429790269
1108	1	0.8834443701441934
1108	2	0.7805656863941017
1108	3	0.7663807989006095
1108	4	0.9596974417449715
1108	5	0.8632856122834305
1108	6	0.8518588820976056
1108	7	0.8130147317247634
1108	8	0.8691444709841853
1108	9	0.9192852686405004
1108	10	0.9932763311251449
1108	11	0.71391581007029
1108	12	0.7865269961203154
1108	13	0.845040104308147
1108	14	0.9064401444582799
1108	15	0.9517446989853156
1108	16	0.7120984674518027
1108	17	0.8718465187119193
1108	18	1.0304271624310957
1108	19	0.866593970191218
1108	20	0.9522044556420539
1108	21	0.9536473240085144
1108	22	0.9327101874255561
1108	23	1.4019130093692382
1108	24	1.184287821228935
1108	25	1.1998288036936202
1109	1	0.6169746743098301
1109	2	0.6219201304813922
1109	3	0.7610519885666207
1109	4	0.8854344424987275
1109	5	0.7625342612311279
1109	6	0.7085869953537305
1109	7	0.6113796730641994
1109	8	0.4721368963141934
1109	9	0.6753535366114036
1109	10	0.6244070132656248
1109	11	0.5002713197795856
1109	12	0.46559619572263955
1109	13	0.619648327735348
1109	14	0.7684926328198927
1109	15	0.5606126349210072
1109	16	0.3007286891455294
1109	17	0.723201469348993
1109	18	0.7179886574579157
1109	19	0.6663212269813741
1109	20	0.5872834248856122
1109	21	0.5358854476921024
1109	22	0.5234886937479715
1109	23	0.9799640439096251
1109	24	0.7356621234682805
1109	25	0.38303315357899237
1110	1	0.4747397420164603
1110	2	0.47163318890774164
1110	3	0.46812551536323
1110	4	0.47603436612801886
1110	5	0.39469763389400114
1110	6	0.4145956056687403
1110	7	0.29398975923627546
1110	8	0.4654475665705604
1110	9	0.5855435121975887
1110	10	0.3982525266242902
1110	11	0.4320298173995525
1110	12	0.4522168469690371
1110	13	0.43681491927198834
1110	14	0.37803704156034
1110	15	0.26199351108879654
1110	16	0.5745218182630367
1110	17	0.5417135087640048
1110	18	-0.16025520221297285
1110	19	0.3774441934733872
1110	20	0.49891534563652584
1110	21	0.6311737609057531
1110	22	0.7127729458043959
1110	23	0.7447567332752101
1110	24	0.7419385826795661
1110	25	0.8539281934806198
1111	1	0.21870887249666993
1111	2	0.18899623967248758
1111	3	0.3923146277560221
1111	4	0.17206145942702378
1111	5	0.2319932087399046
1111	6	0.12802416301288488
1111	7	0.08061437281696371
1111	8	0.3260873476613675
1111	9	0.45297878551009774
1111	10	0.42975250166651335
1111	11	0.06961772381996667
1111	12	-0.22140203438291195
1111	13	0.21368848129299778
1111	14	0.2711329442367756
1111	15	0.2625622694655405
1111	16	0.3183615093198071
1111	17	-0.22670169074167745
1111	18	0.18209130045782018
1111	19	0.13945500933859734
1111	20	0.3579812363487278
1111	21	0.32789068107216224
1111	22	-0.0466399355729763
1111	23	0.3940957241189622
1111	24	0.40720652848676764
1111	25	0.48177357429140244
1112	1	0.28993713946245947
1112	2	0.26779601533149133
1112	3	0.1811536630897308
1112	4	0.2847928110815374
1112	5	0.16749517172363682
1112	6	-0.09519513031296967
1112	7	-0.10502780329071726
1112	8	0.05379346095357107
1112	9	-0.1098366940187887
1112	10	0.06323539566538727
1112	11	0.0014741455810423076
1112	12	0.1089400856395244
1112	13	0.13068231191554958
1112	14	0.07118902948635508
1112	15	-0.20602999077001982
1112	16	0.11252864336355373
1112	17	0.435665106790573
1112	18	0.16891126680667412
1112	19	0.22077862122866776
1112	20	-0.1742677821594043
1112	21	0.009606889709289135
1112	22	-0.1397414498747125
1112	23	-0.010278925348224277
1112	24	0.06536693230057011
1112	25	0.09310279470643934
1113	1	-0.33625418495807224
1113	2	-0.15658421343267648
1113	3	-0.13857366582021768
1113	4	-0.13758335239511155
1113	5	-0.1864426980306284
1113	6	-0.47704539796009726
1113	7	-0.501916029967358
1113	8	-0.3410488069949267
1113	9	-0.5384544930153581
1113	10	-0.45285688161982307
1113	11	-0.5132025231175135
1113	12	-0.7385791569270701
1113	13	-0.46794264144146425
1113	14	-0.2117366979899984
1113	15	-0.5833551173190585
1113	16	-0.6323102633670317
1113	17	-0.5616652418245565
1113	18	-0.5360043276531314
1113	19	-0.9890750407614135
1113	20	-0.7032674609763561
1113	21	-0.526899509107821
1113	22	-1.271876284400495
1113	23	-5.148939393950716
1113	24	-2.4812771920144847
1113	25	-1.5015846623649658
1114	1	-0.7331575123618883
1114	2	-0.9874845462473374
1114	3	-0.7677040924519005
1114	4	-0.6375692036157439
1114	5	-0.5866129785903134
1114	6	-1.2950652126181623
1114	7	-1.2023005937905538
1114	8	-1.0693119488233513
1114	9	-1.4953366184732921
1114	10	-1.0409353323567814
1114	11	-1.240285656746721
1114	12	-2.143167393844138
1114	13	-1.2837462266714113
1114	14	-3.413347784124582
1114	15	-1.8646118048280584
1114	16	-1.935186816328212
1114	17	-1.7433412284101024
1114	18	-1.783711429526818
1114	19	-1.8118695378766532
1114	20	-1.3810270654330843
1114	21	-1.2203520553398377
1114	22	-1.3726014447150057
1114	23	-5.8223058024139505
1114	24	-3.1969808161182023
1114	25	-3.063342846284243
1115	1	-1.781012953675095
1115	2	-1.7507983153975246
1115	3	-1.4508531163469272
1115	4	-1.5535548415766378
1115	5	-1.38767871420794
1115	6	-1.4746129699271755
1115	7	-2.1166327025460525
1115	8	-2.549792113927168
1115	9	-3.8250428311346596
1115	10	-4.535706296293348
1115	11	-3.2649102582493903
1115	12	-3.5435270675844457
1115	13	-2.8309831514392307
1115	14	-3.6429096102081076
1115	15	-3.2356910657182425
1115	16	-3.427142704350956
1115	17	-3.2913895374302164
1115	18	-5.90230799048047
1115	19	-3.7927992243873696
1115	20	-4.082704410592115
1115	21	-3.6675319690173858
1115	22	-3.135966827679127
1115	23	-3.8560439692158326
1115	24	-3.601668784664536
1115	25	-3.680769085237518
1116	1	-0.8820187467589342
1116	2	-0.8063165799213508
1116	3	-1.0876636956999164
1116	4	-0.9199656318122109
1116	5	-0.7814430877423462
1116	6	-3.2675471659567528
1116	7	-1.6276087122061327
1116	8	-2.129927135760049
1116	9	-3.0794691566941452
1116	10	-2.331201558845475
1116	11	-1.8258416697540305
1116	12	-1.541117083698488
1116	13	-2.845277439407628
1116	14	-6.2621911503991825
1116	15	-4.140280941829044
1116	16	-2.7785104122935422
1116	17	-4.019722956377041
1116	18	-2.4833803002131134
1116	19	-2.1782841255483874
1116	20	-2.75203401664486
1116	21	-3.8405083501968633
1116	22	-3.233695998095879
1116	23	-2.2394204762111762
1116	24	-3.284025886749923
1116	25	-3.2146922451841
1201	1	0.7484479130476096
1201	2	0.8871897729127802
1201	3	1.2361666083479186
1201	4	1.2671334572357864
1201	5	0.7066480562010646
1201	6	1.2597395564574292
1201	7	1.2124624796137127
1201	8	1.2491762101742445
1201	9	1.7986294070129176
1201	10	1.6252904635546273
1201	11	1.7871486033149324
1201	12	1.6132284877316323
1201	13	1.8011090815356
1201	14	1.959414713922044
1201	15	1.9355568957160472
1201	16	1.809074346320422
1201	17	2.061391443380323
1201	18	1.8653136589077803
1201	19	2.0144010435897286
1201	20	2.0327277114560047
1201	21	2.125928186452434
1201	22	1.866016418364083
1201	23	2.2748809828284777
1201	24	2.1172935584467254
1201	25	2.224118263800854
1202	1	1.1881863776391697
1202	2	1.1555620004793639
1202	3	1.1630644260551648
1202	4	1.3561007362988278
1202	5	1.2472385164065152
1202	6	1.4537240402483214
1202	7	1.6366147725683504
1202	8	1.5000578053199227
1202	9	1.7518734988791067
1202	10	1.5844074557040244
1202	11	1.6385883850856615
1202	12	1.742997460022437
1202	13	1.7496156506135065
1202	14	2.273091547579405
1202	15	1.9369314239735047
1202	16	1.6080697281207463
1202	17	2.047720580083073
1202	18	2.0253494137967465
1202	19	2.02179579707272
1202	20	1.8417040217398295
1202	21	2.002974001818629
1202	22	2.0009024938025206
1202	23	2.097727280532496
1202	24	2.079484613333676
1202	25	2.507542694178049
1203	1	1.2039604271461108
1203	2	1.3420584554413821
1203	3	1.2333113261093445
1203	4	1.4321490327231885
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2211	17	1.1775784675904788E-4
2211	18	-0.5119120508939048
2211	19	-0.30682702032809317
2211	20	-0.4120958657134821
2211	21	-0.24536815702431625
2211	22	-0.19016271186983857
2211	23	-0.030868257943588162
2211	24	-0.4702888186330796
2211	25	0.03282458696359214
2212	1	-0.13098426750183023
2212	2	-0.032386205419150826
2212	3	-0.42702152625027523
2212	4	-0.15141882498278747
2212	5	-0.3765793717832935
2212	6	-0.47862903418420544
2212	7	-0.20990689822988529
2212	8	-0.17184074802399607
2212	9	-0.22565627391677623
2212	10	-0.5322860217887389
2212	11	-0.7563926714185492
2212	12	-0.053081769910228616
2212	13	-0.1056201610159988
2212	14	-0.04978987853416328
2212	15	-0.4058662720380255
2212	16	-0.5016164234029858
2212	17	-0.1561673299188513
2212	18	0.056097058286027845
2212	19	-0.32942926101740255
2212	20	-0.09455400677905601
2212	21	0.09929357491659374
2212	22	0.13335220670014536
2212	23	0.21664238057895346
2212	24	0.12636422650100343
2212	25	0.12804860309824306
2213	1	-0.20129443483953935
2213	2	-0.01219888419820947
2213	3	-0.28688421985646784
2213	4	-0.064227124916858
2213	5	-0.11311852509789944
2213	6	0.020101233244300687
2213	7	-0.15511070253617376
2213	8	0.009294348056851476
2213	9	-0.45482058403724324
2213	10	-0.018135535040933348
2213	11	-0.10865633148892329
2213	12	-0.10285661147389646
2213	13	-0.13949634069029315
2213	14	-0.26067044724391764
2213	15	-0.07476261355736824
2213	16	-0.04799172414923447
2213	17	-0.11249963707184918
2213	18	-0.17736915877710402
2213	19	-0.09279686490160799
2213	20	0.10702979608018381
2213	21	-0.12900403572388797
2213	22	-0.24204738710771068
2213	23	-0.1541918826347768
2213	24	0.16475886060707978
2213	25	0.3216787440630142
2214	1	-2.8696315102758163
2214	2	-1.5552918802079496
2214	3	-1.7366918072705246
2214	4	-3.482345716767284
2214	5	-2.003711591933257
2214	6	-1.5177108611893786
2214	7	-1.4277134884394727
2214	8	-1.2172092081064285
2214	9	-1.0036178215870812
2214	10	-0.9622519702064452
2214	11	-0.9989341794946363
2214	12	-1.0844478676836893
2214	13	-1.4800615972637168
2214	14	-1.3875684393465875
2214	15	-1.6899417449950107
2214	16	-1.5030988608971896
2214	17	-3.7120096313735225
2214	18	-3.540262595089093
2214	19	-2.384515574950129
2214	20	-2.130775556471818
2214	21	-1.9950455363089645
2214	22	-3.017326305566449
2214	23	-1.9140774356071404
2214	24	-3.070429350854191
2214	25	-3.0664036890677373
2215	1	-1.9741967258713977
2215	2	-3.6377056754973722
2215	3	-2.237910590185777
2215	4	-4.095447215287244
2215	5	-2.7459123513841774
2215	6	-2.3105007646837485
2215	7	-2.688425840111318
2215	8	-1.9428432028616953
2215	9	-4.303210203103692
2215	10	-2.6739867106390136
2215	11	-2.155140578638022
2215	12	-1.9169193412094288
2215	13	-2.1330027646942717
2215	14	-1.7953528016961577
2215	15	-1.7803952428894902
2215	16	-1.8795967421224766
2215	17	-2.4431202250934057
2215	18	-1.9874860003314794
2215	19	-2.1352560751380985
2215	20	-2.024642991233936
2215	21	-2.741889561871421
2215	22	-2.704466104740529
2215	23	-2.712924466393005
2215	24	-2.413788227818106
2215	25	-3.5255337840826684
2216	1	-3.8038167436854344
2216	2	-3.90595533222578
2216	3	-5.250920844653056
2216	4	-6.185952812086036
2216	5	-5.675279948255138
2216	6	-4.69746696074073
2216	7	-6.528184714370411
2216	8	-4.042863547519669
2216	9	-4.915842811341069
2216	10	-3.7503170423637684
2216	11	-4.121934393482167
2216	12	-4.442634205486581
2216	13	-3.9970631785952975
2216	14	-5.147499364897062
2216	15	-4.500035292001542
2216	16	-4.710531210008778
2216	17	-4.44537119413534
2216	18	-3.9970958244510832
2216	19	-4.722101532653134
2216	20	-5.4246668018429745
2216	21	-5.131723951132859
2216	22	-4.680066996202136
2216	23	-4.656832914957345
2216	24	-5.07282968344159
2216	25	-5.134900326924672
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145434.0
3	0.0
4	1166.0
5	7265.0
6	8674.0
7	7716.0
8	6799.0
9	6208.0
10	5673.0
11	5315.0
12	5081.0
13	5099.0
14	4987.0
15	5047.0
16	5234.0
17	5811.0
18	6392.0
19	7872.0
20	9918.0
21	12606.0
22	15800.0
23	18816.0
24	21683.0
25	24580.0
26	26825.0
27	30050.0
28	33476.0
29	38858.0
30	46928.0
31	57700.0
32	71219.0
33	89379.0
34	113028.0
35	144881.0
36	191152.0
37	284955.0
38	705440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39032751359378	17.564622890647826	12.272887744257151	24.772161851501238
2	15.961343753387958	19.460196212103114	39.65021581861314	24.928244215895788
3	19.763632237597054	24.587043184179798	28.11721643356354	27.532108144659606
4	13.226284065219998	15.544209733753458	36.06582455024929	35.16368165077726
5	14.281153183216402	37.93637609840536	33.54073333602843	14.241737382349806
6	33.70752478788539	34.64350908839331	17.582999020390677	14.065967103330626
7	30.03410411984571	31.11340136995684	20.38680755742515	18.465686952772298
8	27.64066373862441	34.21836721181222	19.690256534757626	18.45071251480574
9	28.190856040608764	14.634159796330978	18.812675436056	38.362308727004255
10	17.115989709092446	27.925856181072895	31.872297855202735	23.08585625463192
11	36.23417132323026	21.999131132355206	22.6392429358532	19.127454608561333
12	24.628101909387212	24.08681231324635	29.329684582602873	21.955401194763567
13	29.674282659193647	20.913018074363386	25.17345955092905	24.239239715513925
14	22.997436571699676	20.729933450164857	26.069734812055103	30.202895166080364
15	24.933433489574618	28.38133048433572	22.642175584801507	24.04306044128815
16	24.257698162076878	26.93456820828355	25.030353153946695	23.777380475692883
17	23.498008931646194	27.01429863404573	25.814737997841803	23.672954436466277
18	23.341673931355132	26.439557791976103	27.089747409540838	23.129020867127927
19	24.674578901972758	26.075020643412405	26.303017708277775	22.94738274633706
20	25.318312765337264	25.615453720399696	26.22587310154837	22.840360412714674
21	25.282299140552883	25.36398706967884	25.768013636413784	23.58570015335449
22	25.208148649493673	25.893471295993177	25.58936368217582	23.309016372337336
23	24.557037334012563	25.622501740218684	26.031050834615314	23.78941009115344
24	24.397550479973518	25.725523189525173	26.116765971532608	23.7601603589687
25	24.36265520144063	25.694788927879685	26.178294256460568	23.764261614219112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	277.0
2	595.5
3	914.0
4	914.0
5	914.0
6	1492.5
7	2071.0
8	2071.0
9	2071.0
10	2338.5
11	2606.0
12	2606.0
13	2606.0
14	3875.0
15	5144.0
16	5144.0
17	5144.0
18	10114.0
19	15084.0
20	15084.0
21	15084.0
22	24776.0
23	34468.0
24	34468.0
25	34468.0
26	52563.0
27	70658.0
28	70658.0
29	70658.0
30	92829.0
31	115000.0
32	115000.0
33	115000.0
34	137160.5
35	159321.0
36	159321.0
37	159321.0
38	181035.0
39	202749.0
40	202749.0
41	202749.0
42	223356.5
43	243964.0
44	243964.0
45	243964.0
46	266756.0
47	289548.0
48	289548.0
49	289548.0
50	292367.5
51	295187.0
52	295187.0
53	295187.0
54	275529.5
55	255872.0
56	255872.0
57	255872.0
58	226840.5
59	197809.0
60	197809.0
61	197809.0
62	168488.5
63	139168.0
64	139168.0
65	139168.0
66	109418.0
67	79668.0
68	79668.0
69	79668.0
70	59604.0
71	39540.0
72	39540.0
73	39540.0
74	28562.0
75	17584.0
76	17584.0
77	17584.0
78	12317.5
79	7051.0
80	7051.0
81	7051.0
82	4716.0
83	2381.0
84	2381.0
85	2381.0
86	1589.5
87	798.0
88	798.0
89	798.0
90	474.5
91	151.0
92	151.0
93	151.0
94	96.0
95	41.0
96	41.0
97	41.0
98	27.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3364618544123814
2	0.010978072792431286
3	0.009416338587650265
4	0.01148333974103691
5	0.012631673715140599
6	0.07854604382869246
7	0.07670870947012656
8	0.10266105728486997
9	0.08143984544343376
10	0.08924851646733886
11	0.08369058003267699
12	0.09535765320957049
13	0.08157764552032619
14	0.08520638087849387
15	0.0826341127765016
16	0.08451738049403165
17	0.09664378726056662
18	0.09935385543945133
19	0.09365811892789702
20	0.09209638472311601
21	0.0787757106235132
22	0.10008878918287771
23	0.0943011859533951
24	0.08943224990319544
25	0.08773271562152198
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2177067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.46613888593784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79988498331981	44.133928568755074
2	12.698898786324461	14.595133627067778
3	4.5335527226184835	7.815773112141461
4	2.0816437126562044	4.7849610681016275
5	1.1169029109139088	3.2092048900343464
6	0.6630977114704182	2.2863399109383944
7	0.42608009707307765	1.7139624624454748
8	0.32503066077918435	1.4942605675619802
9	0.2178115752586685	1.1265111211301593
>10	1.0400763441095169	10.36410076221882
>50	0.06069251514076928	2.3992019003835328
>100	0.03172928688200102	3.463092775600507
>500	0.003469189798188321	1.418893556362936
>1k	0.0011295036552240265	1.194635677257938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4352	0.19990197821197053	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2614	0.12006980033228193	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2422	0.11125059541116558	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2415	0.11092906189841653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5933358964147634E-5
2	0.0	0.0	0.0	0.0	4.5933358964147634E-5
3	0.0	0.0	0.0	0.0	4.5933358964147634E-5
4	0.0	0.0	0.0	0.0	4.5933358964147634E-5
5	0.0	0.0	0.0	0.0	4.5933358964147634E-5
6	0.0	0.0	0.0	0.0	4.5933358964147634E-5
7	0.0	0.0	0.0	0.0	4.5933358964147634E-5
8	0.0	0.0	0.0	0.0	4.5933358964147634E-5
9	0.0	0.0	0.0	0.0	4.5933358964147634E-5
10	0.0	0.0	0.0	0.0	4.5933358964147634E-5
11	0.0	4.5933358964147634E-5	0.0	0.0	4.5933358964147634E-5
12	0.0	4.5933358964147634E-5	0.0	0.0	1.378000768924429E-4
13	0.0	4.5933358964147634E-5	0.0	0.0	1.378000768924429E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	40	0.0052841576	14.24831	4
GCACCGT	115	5.336915E-9	12.389835	6
GTCCTAC	840	0.0	11.675617	1
GGTATCA	1505	0.0	11.641347	1
GTATCAA	3445	0.0	11.442819	1
GCCTACG	100	1.8778974E-6	11.426235	1
ATACCGT	60	0.005882128	11.082019	6
CCGTCCG	60	0.005883135	11.081764	9
GTCCTAA	535	0.0	11.034681	1
ATTAGAC	70	0.0014932936	10.855856	3
ACCGTCC	70	0.0014932936	10.855856	8
CGAAATC	115	8.836869E-7	10.73761	13
GTACTGG	840	0.0	10.201996	1
TAGGACC	310	0.0	10.111704	4
TGGACCG	160	6.186383E-9	10.092553	5
GTAGGAC	705	0.0	9.9704485	3
TACCGTG	115	1.0281914E-5	9.911868	7
TCCAACG	125	2.5746613E-6	9.878828	18
TAGAGTG	280	0.0	9.8381195	5
ATTTCTC	1065	0.0	9.810818	15
>>END_MODULE