FastQCFastQC Report
Thu 26 May 2016
SRR1512862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177067
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60690.27876955555341204No Hit
TATCAACGCAGAGTACTTTTTTTTT42610.19572204254623307No Hit
GGTATCAACGCAGAGTACTTTTTTT33590.1542901527605719No Hit
GTACTGGTTCACTATCGGTCAGTCA21960.10086965628526821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAA701.0801224E-412.2277481
GGTATCA17300.012.1492131
TATACCG550.003047581812.1022845
ACCGTAT550.003068122512.0914358
TAGGACC3950.011.79589754
CGTAAAG600.00590525611.07617813
ACCGTGC1303.2658136E-810.9620168
TAGAGGT2700.010.5654874
CGAAATC909.58069E-510.54874113
CTACACG1002.3748058E-510.4598334
TATCGCG1553.6252459E-910.4260417
GTATCAA35950.010.4231591
TAGGACA3650.010.4207544
TTAGGAC5500.010.3736273
GATATAC2950.010.31644251
GTCCTAC7750.010.3081231
TCCTAGG2550.010.068522
CCACGAC856.5370405E-410.068523
TACACCG951.628976E-410.0094095
TCTACAC2800.09.848773
Produced by FastQC (version 0.11.5)