##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2177067 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.33300445048315 31.0 30.0 34.0 26.0 34.0 2 30.31334129817778 31.0 30.0 34.0 26.0 34.0 3 30.507994012127327 31.0 30.0 34.0 27.0 34.0 4 34.14060936112669 37.0 35.0 37.0 30.0 37.0 5 33.74600873560621 37.0 35.0 37.0 28.0 37.0 6 33.631115624829185 37.0 35.0 37.0 28.0 37.0 7 33.47249120031675 37.0 35.0 37.0 28.0 37.0 8 33.42244404972378 37.0 35.0 37.0 27.0 37.0 9 34.77424672736301 39.0 35.0 39.0 27.0 39.0 10 34.682679035601566 39.0 35.0 39.0 27.0 39.0 11 34.70108591053927 39.0 35.0 39.0 27.0 39.0 12 34.561685974754106 39.0 35.0 39.0 27.0 39.0 13 34.557912549315205 39.0 35.0 39.0 27.0 39.0 14 35.64708527574025 40.0 36.0 41.0 27.0 41.0 15 35.62827602457802 40.0 36.0 41.0 27.0 41.0 16 35.6191068074616 40.0 36.0 41.0 27.0 41.0 17 35.593933030081295 40.0 36.0 41.0 27.0 41.0 18 35.50464592959243 40.0 36.0 41.0 27.0 41.0 19 35.50789984874145 40.0 36.0 41.0 26.0 41.0 20 35.47838215360391 40.0 36.0 41.0 26.0 41.0 21 35.44603588222135 40.0 36.0 41.0 26.0 41.0 22 35.405740383736465 40.0 36.0 41.0 26.0 41.0 23 35.25190772723118 40.0 35.0 41.0 25.0 41.0 24 35.203436550184264 40.0 35.0 41.0 25.0 41.0 25 35.13620021799972 40.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6683703388097264 1101 2 0.6391220400896849 1101 3 0.6061766967835851 1101 4 0.6153279925891084 1101 5 0.6528617148593483 1101 6 0.6921232583134369 1101 7 0.7635007435560581 1101 8 0.4984979073125686 1101 9 0.863282236755019 1101 10 0.4711216855769891 1101 11 0.8649176001963497 1101 12 1.0007538648098446 1101 13 1.0946313998606243 1101 14 1.1460514979774956 1101 15 0.8926270671764556 1101 16 1.0905579722774021 1101 17 1.2008823352062095 1101 18 1.0271534493975452 1101 19 0.8911873031469 1101 20 1.068869612998114 1101 21 1.1192908563103003 1101 22 0.8881256434407874 1101 23 1.0792631637489691 1101 24 1.1325532766729793 1101 25 1.2554421887107523 1102 1 0.7898486215112079 1102 2 0.8542657341226594 1102 3 0.5253187170363276 1102 4 0.6302423787302089 1102 5 0.7041746280738437 1102 6 0.8331102865166358 1102 7 0.8192377926815126 1102 8 0.8920943588984755 1102 9 0.9929136703477894 1102 10 0.8931590108761753 1102 11 1.0654933805963793 1102 12 1.0620759838156246 1102 13 1.1669544394455258 1102 14 1.0253423172929175 1102 15 1.166097483110839 1102 16 1.184870132290797 1102 17 1.2331864610744816 1102 18 0.6489196222990401 1102 19 0.9457647573271615 1102 20 1.0363622902095955 1102 21 0.9891656575489023 1102 22 0.8547780829754785 1102 23 1.1758486520743503 1102 24 1.1632927527797676 1102 25 1.204158566650328 1103 1 0.6524794884921441 1103 2 0.8508240452519864 1103 3 0.5804144333716401 1103 4 0.5450708271827551 1103 5 0.6591453187891503 1103 6 0.6569401696894133 1103 7 0.7735026010438801 1103 8 0.8840263121675846 1103 9 0.8415125635636116 1103 10 0.8186552191155698 1103 11 0.9679774961938392 1103 12 1.0895615341333098 1103 13 1.0299698114479838 1103 14 1.1254107739438695 1103 15 1.2110128346173 1103 16 1.1107860986773517 1103 17 1.074825731416766 1103 18 1.0873979990155789 1103 19 1.1235335624101737 1103 20 1.0399347435811848 1103 21 1.1648545282191236 1103 22 1.1407936599379696 1103 23 1.2940491029864205 1103 24 1.1949127568789706 1103 25 1.1676240308848023 1104 1 0.9198463539375119 1104 2 0.9756534892246975 1104 3 0.8728198508231735 1104 4 0.7994986145578693 1104 5 0.8492041065318929 1104 6 0.7910785404848895 1104 7 0.7450473164910321 1104 8 0.7854481003950724 1104 9 0.8102742145654744 1104 10 0.9422723895609835 1104 11 1.0005954214127115 1104 12 0.9879444645412434 1104 13 0.8591494507833985 1104 14 1.132396739061626 1104 15 1.133882063609704 1104 16 1.0484778420413647 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0.908510848954819 1107 17 0.8258476312029117 1107 18 0.9092950781417599 1107 19 0.8688219953167007 1107 20 0.919347861796723 1107 21 0.8819019822674647 1107 22 0.9116133453823281 1107 23 0.9479986441466508 1107 24 0.9806927844905644 1107 25 1.018671647354303 1108 1 0.5283605964781088 1108 2 0.4341150541370986 1108 3 0.4683024695937945 1108 4 0.3563154721630326 1108 5 0.522850200746042 1108 6 0.4720904285313168 1108 7 0.5053674512846342 1108 8 0.4189715603266464 1108 9 0.5668648879030513 1108 10 0.548899693669938 1108 11 0.5287605206453279 1108 12 0.6479598045722312 1108 13 0.5715950299698562 1108 14 0.6677077042227566 1108 15 0.7606433971799191 1108 16 0.6617053711883756 1108 17 0.6195407756731086 1108 18 0.7229264422131934 1108 19 0.7226629801377698 1108 20 0.7978815263792143 1108 21 0.9022646265383472 1108 22 0.8038818660219746 1108 23 0.8293047438623731 1108 24 0.8116885485032839 1108 25 0.946940449748972 1109 1 0.2757953420115449 1109 2 0.24562764680127813 1109 3 0.2925500038985369 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2216 20 -3.055314356496993 2216 21 -3.963970783854009 2216 22 -3.158474697178498 2216 23 -4.731315590868338 2216 24 -3.845837700599187 2216 25 -4.0252657276182795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54034.0 3 0.0 4 889.0 5 7525.0 6 9123.0 7 7081.0 8 5627.0 9 5070.0 10 4299.0 11 3999.0 12 3612.0 13 3513.0 14 3426.0 15 3508.0 16 3606.0 17 3742.0 18 4170.0 19 5283.0 20 6704.0 21 8668.0 22 11142.0 23 13735.0 24 16433.0 25 18677.0 26 20882.0 27 23420.0 28 26327.0 29 31861.0 30 40326.0 31 51075.0 32 64520.0 33 82793.0 34 107681.0 35 140705.0 36 189962.0 37 292051.0 38 901598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.306415627630535 18.651298080658414 13.268186024743777 26.77410026696727 2 14.609257068020726 19.981894838420626 38.27789584936873 27.13095224418992 3 17.92593082638234 24.434456701711056 28.95747077643444 28.68214169547216 4 12.541870545369022 16.173192928815936 35.34337981430884 35.9415567115062 5 14.004442514913665 38.07028545570233 33.62473842224106 14.30053360714294 6 33.82693669764365 35.063871368354384 17.171017524926448 13.938174409075513 7 29.68539553099847 31.196820858405005 20.482617229697116 18.63516638089941 8 27.47591515074085 34.75603563128431 19.375556365417822 18.39249285255702 9 27.61575940512863 14.485770270673825 18.82013502679632 39.07833529740123 10 16.454212232007777 28.38856321427061 32.06242107062697 23.09480348309464 11 36.17319860024089 21.893744723112103 22.578511364085283 19.354545312561726 12 24.133223644169824 24.33675491867247 29.566787517667166 21.963233919490545 13 29.498773726745252 20.792624077768554 25.328296249611267 24.38030594587493 14 22.624243587894465 20.592369321110088 26.307891306324972 30.475495784670475 15 24.767463494973022 28.49654646480656 22.666354275056218 24.0696357651642 16 24.09836916550942 26.96667357263509 25.06186660517139 23.873090656684095 17 23.229761965462735 27.09672647962542 25.970289666336388 23.703221888575456 18 23.136986490148974 26.40112451939199 27.33294134400279 23.12894764645625 19 24.489901848020608 26.021666195076598 26.4789401350095 23.009491821893295 20 25.115741963518374 25.466631083199626 26.466106977314617 22.95151997596738 21 25.01019721974182 25.179783414727357 26.07989475734292 23.730124608187907 22 24.828598962453 25.77992869275876 25.98300091224028 23.408471432547962 23 24.182305827059984 25.50959616768799 26.327118090531894 23.980979914720127 24 24.229249719921345 25.71105988010475 26.340300964554604 23.719389435419306 25 23.996597256767934 25.583686675697166 26.543693878047854 23.87602218948705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 68.0 1 68.0 2 230.0 3 392.0 4 392.0 5 392.0 6 977.5 7 1563.0 8 1563.0 9 1563.0 10 1928.5 11 2294.0 12 2294.0 13 2294.0 14 3590.5 15 4887.0 16 4887.0 17 4887.0 18 10211.0 19 15535.0 20 15535.0 21 15535.0 22 25800.5 23 36066.0 24 36066.0 25 36066.0 26 54993.5 27 73921.0 28 73921.0 29 73921.0 30 95940.5 31 117960.0 32 117960.0 33 117960.0 34 139764.5 35 161569.0 36 161569.0 37 161569.0 38 182574.0 39 203579.0 40 203579.0 41 203579.0 42 224798.0 43 246017.0 44 246017.0 45 246017.0 46 268355.5 47 290694.0 48 290694.0 49 290694.0 50 292971.5 51 295249.0 52 295249.0 53 295249.0 54 274884.5 55 254520.0 56 254520.0 57 254520.0 58 225527.0 59 196534.0 60 196534.0 61 196534.0 62 166427.0 63 136320.0 64 136320.0 65 136320.0 66 106666.5 67 77013.0 68 77013.0 69 77013.0 70 57255.5 71 37498.0 72 37498.0 73 37498.0 74 26785.0 75 16072.0 76 16072.0 77 16072.0 78 11252.0 79 6432.0 80 6432.0 81 6432.0 82 4264.0 83 2096.0 84 2096.0 85 2096.0 86 1369.0 87 642.0 88 642.0 89 642.0 90 385.5 91 129.0 92 129.0 93 129.0 94 72.0 95 15.0 96 15.0 97 15.0 98 8.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08699778187809562 2 0.040972556196019694 3 0.018327410226694908 4 0.005466069716733569 5 0.01708720953466292 6 0.06274496834502566 7 0.07519290862430968 8 0.05116976188606047 9 0.021221211841436206 10 0.049194627450602116 11 0.005557936434661864 12 2.7560015378488584E-4 13 0.006706270408765555 14 5.971336665339193E-4 15 0.014331207996814062 16 0.009462271946614413 17 0.00523640292191283 18 0.0062928701780882255 19 0.0016076675637451672 20 0.0011942673330678387 21 1.3780007689244292E-4 22 4.134002306773287E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2177067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.22052557570226 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95017019085154 40.096170941606005 2 14.161351862110955 15.356718816522118 3 5.098633355557284 8.293517407683668 4 2.3058938283158503 5.001071011722142 5 1.3055722105460819 3.539440571641998 6 0.7797681008081693 2.5367661751791672 7 0.4816237350567229 1.8279724431155806 8 0.3448105380342634 1.4956646877006752 9 0.25233804567567436 1.2313711313352564 >10 1.2101936093272707 11.327642823175013 >50 0.06684861185395834 2.4781686487746835 >100 0.03759891144237834 3.8142637679877134 >500 0.003578262584105255 1.328635101102276 >1k 0.0015335411074735858 1.392244395804174 >5k 8.519672819297698E-5 0.2803520766493756 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6069 0.27876955555341204 No Hit TATCAACGCAGAGTACTTTTTTTTT 4261 0.19572204254623307 No Hit GGTATCAACGCAGAGTACTTTTTTT 3359 0.1542901527605719 No Hit GTACTGGTTCACTATCGGTCAGTCA 2196 0.10086965628526821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 4.5933358964147634E-5 11 0.0 4.5933358964147634E-5 0.0 0.0 4.5933358964147634E-5 12 0.0 4.5933358964147634E-5 0.0 0.0 4.5933358964147634E-5 13 0.0 4.5933358964147634E-5 0.0 0.0 4.5933358964147634E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTAA 70 1.0801224E-4 12.227748 1 GGTATCA 1730 0.0 12.149213 1 TATACCG 55 0.0030475818 12.102284 5 ACCGTAT 55 0.0030681225 12.091435 8 TAGGACC 395 0.0 11.7958975 4 CGTAAAG 60 0.005905256 11.076178 13 ACCGTGC 130 3.2658136E-8 10.962016 8 TAGAGGT 270 0.0 10.565487 4 CGAAATC 90 9.58069E-5 10.548741 13 CTACACG 100 2.3748058E-5 10.459833 4 TATCGCG 155 3.6252459E-9 10.426041 7 GTATCAA 3595 0.0 10.423159 1 TAGGACA 365 0.0 10.420754 4 TTAGGAC 550 0.0 10.373627 3 GATATAC 295 0.0 10.3164425 1 GTCCTAC 775 0.0 10.308123 1 TCCTAGG 255 0.0 10.06852 2 CCACGAC 85 6.5370405E-4 10.06852 3 TACACCG 95 1.628976E-4 10.009409 5 TCTACAC 280 0.0 9.84877 3 >>END_MODULE