FastQCFastQC Report
Thu 26 May 2016
SRR1512861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370889
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51950.37895117693700947No Hit
TATCAACGCAGAGTACTTTTTTTTT29890.2180336993002351No Hit
GGTATCAACGCAGAGTACTTTTTTT28510.20796723877717307No Hit
GTACTGGTTCACTATCGGTCAGTCA23460.17112982889205472No Hit
GTCCTACAGTGGACATTTCTAAATT18370.1340006375424998No Hit
GTCCTAAAGTGTGTATTTCTCATTT17130.12495541214496578No Hit
CTGTAGGACGTGGAATATGGCAAGA15470.11284648137084767No Hit
CTTTAGGACGTGAAATATGGCGAGG15030.10963688526204529No Hit
GGATACCACGTGTCCCGCCCTACTC14440.10533310866160572No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13900.10139405889171187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG500.001501039813.298058519
GGTATCA12550.012.6724331
ATAGGAC751.4810566E-512.6643553
GCGCCAC1155.329639E-912.38994713
GTCCTAA5700.012.3635761
GTTTAAG1501.6916601E-1011.4279551
TGCGATA1251.8344508E-811.39833510
TAGGACC2600.011.3248564
AGGACCG855.333443E-511.1740245
ATCCCGT600.00588144611.08171510
ATTTCTC11450.010.95076615
CGGTCTT700.00149211510.8563517
GTATCAA26550.010.7607861
GTCTTAC1252.1642336E-710.6660911
TCCTAAA7500.010.6380592
TAGCACC909.529882E-510.5536294
GTCCTAC8200.010.4523981
TAGTACT1002.4026374E-510.4480924
TAAAGTG6850.010.3991815
GTGTATT7050.010.37437211
Produced by FastQC (version 0.11.5)