FastQCFastQC Report
Thu 26 May 2016
SRR1512861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370889
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62990.4594828611215058No Hit
TATCAACGCAGAGTACTTTTTTTTT48470.3535661895310269No Hit
GGTATCAACGCAGAGTACTTTTTTT36780.2682930565494362No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22300.16266816642339388No Hit
GTACTGGTTCACTATCGGTCAGTCA20680.15085101711371235No Hit
GTCCTACAGTGGACATTTCTAAATT16750.12218348823281827No Hit
GTCCTAAAGTGTGTATTTCTCATTT15800.11525367845244947No Hit
CTTTAGGACGTGAAATATGGCGAGG15270.11138757404866477No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15120.1102933935570276No Hit
CTGTAGGACGTGGAATATGGCAAGA14360.10474954573273255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACT400.00529997814.24135413
GGTATCA11750.014.1636031
ATAGGAC1152.5465852E-1114.0580353
CTTATAC1500.013.9477771
CCGTATG500.001500474513.2987229
GTTGGCG500.001506261913.29193113
CTATTAG751.4626152E-512.6797971
ACACCGA604.0646314E-412.6765546
GGCGAGG4850.011.93946219
TAGGACT1459.640644E-1111.8023094
GGTTAGA657.952928E-411.7044281
TGTACCG657.9700106E-411.7014355
TGTAGGA8950.011.2630592
GTAGGAC8550.011.0114023
GTATCAA26550.010.996321
TAATAGT1303.2263415E-810.9700964
TAGGACG10900.010.9034
AGGACGT11100.010.8778545
GGACGTG10850.010.8656186
TCGGGCT700.001498775710.85055513
Produced by FastQC (version 0.11.5)