##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512861_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1370889
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.016290159159496	31.0	30.0	34.0	26.0	34.0
2	30.033214213550476	31.0	30.0	34.0	26.0	34.0
3	30.225887726869207	31.0	30.0	34.0	26.0	34.0
4	33.821917018810424	37.0	35.0	37.0	30.0	37.0
5	33.3566393778052	37.0	35.0	37.0	27.0	37.0
6	33.20413541869546	37.0	35.0	37.0	27.0	37.0
7	33.03946563142603	37.0	35.0	37.0	26.0	37.0
8	32.98148354826685	37.0	35.0	37.0	26.0	37.0
9	34.270304160293065	39.0	35.0	39.0	25.0	39.0
10	34.19625950751666	39.0	35.0	39.0	25.0	39.0
11	34.180234140036134	39.0	35.0	39.0	25.0	39.0
12	34.03751799015092	39.0	35.0	39.0	25.0	39.0
13	34.01847924959643	39.0	35.0	39.0	25.0	39.0
14	35.080611924087215	39.0	35.0	41.0	24.0	41.0
15	35.075278158917314	39.0	35.0	41.0	25.0	41.0
16	35.0604695201435	39.0	35.0	41.0	24.0	41.0
17	35.04868592570223	39.0	35.0	41.0	24.0	41.0
18	34.97326479386734	39.0	35.0	41.0	23.0	41.0
19	34.98431091065724	40.0	35.0	41.0	23.0	41.0
20	34.953469609866296	40.0	35.0	41.0	23.0	41.0
21	34.91746304770116	40.0	35.0	41.0	22.0	41.0
22	34.87735476759971	40.0	35.0	41.0	22.0	41.0
23	34.718896278254476	39.0	35.0	41.0	19.0	41.0
24	34.662305263227	39.0	35.0	41.0	19.0	41.0
25	34.602439730714885	39.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9155896235600096
1101	2	0.6985320611865262
1101	3	0.7715691703829286
1101	4	0.8473748891967858
1101	5	0.7597680250364363
1101	6	0.713505156298389
1101	7	0.8976119466520274
1101	8	0.7115150429535504
1101	9	1.0446676216611976
1101	10	0.8049008711618626
1101	11	1.1312954313777297
1101	12	1.1831406546404324
1101	13	1.1854028479501153
1101	14	1.3662017264869704
1101	15	0.9772805770893882
1101	16	1.176023016942473
1101	17	1.3394154137312668
1101	18	1.3665550567886058
1101	19	1.0928853800414657
1101	20	1.1529008325635317
1101	21	1.2638301803845167
1101	22	1.172490624645775
1101	23	1.4207563813118398
1101	24	1.449321566997689
1101	25	1.532631543964527
1102	1	0.6479642264284173
1102	2	0.7986282091411496
1102	3	0.4772884011154197
1102	4	0.615239200133729
1102	5	0.8569263835366812
1102	6	0.8411023683571628
1102	7	0.8855656120510886
1102	8	0.9109944415011526
1102	9	0.9313346471062758
1102	10	0.8437512146688917
1102	11	0.9558844728406299
1102	12	0.9777828815081335
1102	13	1.008897149314194
1102	14	1.0356477609720969
1102	15	1.109374866851411
1102	16	1.0820542917031304
1102	17	1.1493833030635017
1102	18	0.49554777708146247
1102	19	0.9432556789667856
1102	20	1.0246820485567554
1102	21	0.9205824483183704
1102	22	0.6039661614330285
1102	23	0.9984220058903048
1102	24	1.0297423302032414
1102	25	1.030267683480865
1103	1	0.7569155164868491
1103	2	0.8343109850550583
1103	3	0.5462241263530245
1103	4	0.41869516187087896
1103	5	0.5334348450662105
1103	6	0.6378943900246767
1103	7	0.8102119655698701
1103	8	0.6844597707064892
1103	9	0.6104305480129284
1103	10	0.6651064971083329
1103	11	0.8430933245176249
1103	12	0.9328362242833563
1103	13	0.9273512848861927
1103	14	0.9332186487741154
1103	15	0.9703434219841895
1103	16	0.8638253236337832
1103	17	0.9242401343946014
1103	18	0.9910069214360249
1103	19	1.1075623211178893
1103	20	0.7543071355116808
1103	21	0.8704926134947826
1103	22	0.9380675408351351
1103	23	1.046816721888142
1103	24	1.070571632429555
1103	25	0.932041876597161
1104	1	0.8345272072509964
1104	2	0.9698050889490979
1104	3	0.9202342030325212
1104	4	0.8540820122493855
1104	5	0.7692812660639774
1104	6	0.6564029332015338
1104	7	0.7155481880984311
1104	8	0.7380009701124663
1104	9	0.775192377110983
1104	10	0.9278145326971128
1104	11	0.8395873961090174
1104	12	0.8906356545345204
1104	13	0.7839901770975004
1104	14	1.0504305462752015
1104	15	0.9705267613269797
1104	16	1.1031792008680128
1104	17	1.1953123727147954
1104	18	0.7032108611365828
1104	19	1.2017777902439803
1104	20	1.0897672779750849
1104	21	1.1039536586861871
1104	22	1.0103156827781987
1104	23	0.8989387001720317
1104	24	1.2463818395418969
1104	25	1.061879743505024
1105	1	0.5356760067633317
1105	2	0.4722630359251774
1105	3	0.5713727034951752
1105	4	0.4108702862937008
1105	5	0.4446312140543114
1105	6	0.4528947832345054
1105	7	0.5070752745990887
1105	8	0.5116833722838194
1105	9	0.7789041074606402
1105	10	0.45823038067328525
1105	11	0.7011192988354082
1105	12	0.7922159638660986
1105	13	0.7933594187123489
1105	14	0.9003968627027348
1105	15	1.0106979849033735
1105	16	0.8053943431931643
1105	17	0.6622528519238955
1105	18	0.6566943083881966
1105	19	0.8770177816789442
1105	20	0.8060114192518952
1105	21	0.7928814193727121
1105	22	0.7259448818379326
1105	23	0.8435272408127688
1105	24	0.9305664645289582
1105	25	0.7986482510902064
1106	1	0.06257618221191308
1106	2	0.2914796157301289
1106	3	-1.7534566783634062
1106	4	-0.41418142532196356
1106	5	-0.13583878803312643
1106	6	0.0876382608428159
1106	7	0.27865959084128633
1106	8	0.28952807182164975
1106	9	0.4617825165887979
1106	10	0.30443339383451473
1106	11	0.37393335093862845
1106	12	0.4913910794948251
1106	13	0.5285758915574945
1106	14	0.5792737674929782
1106	15	0.4763628091011327
1106	16	0.6055494349111967
1106	17	0.529636063029777
1106	18	0.699866391188884
1106	19	0.8049592194602511
1106	20	0.6841544417634822
1106	21	0.6443092896477154
1106	22	0.6561234184552731
1106	23	0.7261194462031924
1106	24	0.8618324977175504
1106	25	0.8252476020857031
1107	1	0.5192694313358821
1107	2	0.6629117434637486
1107	3	0.5900414730963028
1107	4	0.3571698180065823
1107	5	0.48446104416834146
1107	6	0.4368159791273456
1107	7	0.5150617077903163
1107	8	0.3780990795431407
1107	9	0.4283339294386508
1107	10	0.319349201696582
1107	11	0.644694112268482
1107	12	0.757476653300337
1107	13	0.631705230803405
1107	14	0.679641925051321
1107	15	0.5782104018640908
1107	16	0.6303333124699506
1107	17	0.6649576539114577
1107	18	0.7795534658781875
1107	19	0.6807995042009267
1107	20	0.7824190879566615
1107	21	0.6290760600255894
1107	22	0.6059871467525824
1107	23	0.6909756459626948
1107	24	0.6827605407456403
1107	25	0.6444696646638803
1108	1	0.6866471643386411
1108	2	0.6711333071138341
1108	3	0.5980937922529606
1108	4	0.5842788135732633
1108	5	0.8025458219255981
1108	6	0.678475564007158
1108	7	0.5988185045097154
1108	8	0.55402397640119
1108	9	0.6529146108875494
1108	10	0.8170473920693766
1108	11	0.6688102904784898
1108	12	0.7219143918220325
1108	13	0.7075200025304014
1108	14	0.7139740474026226
1108	15	0.8327209472116834
1108	16	0.686347323632063
1108	17	0.7154271647589496
1108	18	0.7679444768615369
1108	19	0.8819984567021706
1108	20	0.9463056514651811
1108	21	0.924610540897163
1108	22	0.9406549267846103
1108	23	1.0450456907860755
1108	24	1.0598911514330212
1108	25	1.0299902383287574
1109	1	0.3493661860863817
1109	2	0.37017585749688564
1109	3	0.517938775595006
1109	4	0.3193792100906734
1109	5	0.06706189645608873
1109	6	0.4218110620826465
1109	7	0.3957266191248934
1109	8	0.6271394627553946
1109	9	0.7696231468354711
1109	10	0.29291072820103636
1109	11	0.6489088126459777
1109	12	0.9221633899688015
1109	13	0.750486649661589
1109	14	0.9946980216580315
1109	15	0.8776846481815923
1109	16	0.8713972974967632
1109	17	0.9110683244271627
1109	18	1.1120787119947764
1109	19	0.9623336221465522
1109	20	1.0721939613295461
1109	21	1.1929654952527784
1109	22	1.1414004428644162
1109	23	0.9225174831397851
1109	24	1.0113449605872376
1109	25	0.9395314713870135
1110	1	0.5302779070966253
1110	2	0.44036430069025556
1110	3	0.4700757683231309
1110	4	0.4664524761787163
1110	5	0.5855324570941391
1110	6	0.6097381869555818
1110	7	0.4632846768307317
1110	8	0.5570323394463017
1110	9	0.47977405777456283
1110	10	0.6859641334711952
1110	11	0.6733454378707862
1110	12	0.7869565716092737
1110	13	0.7214273017845088
1110	14	0.7883300491816883
1110	15	0.7360566866521552
1110	16	0.5091265961127576
1110	17	0.6599744155992653
1110	18	0.6296735200329309
1110	19	0.7848529747151645
1110	20	0.8119296717056486
1110	21	0.8781668401585563
1110	22	0.9190218362493567
1110	23	0.979732277103345
1110	24	0.9212206349949312
1110	25	1.010248493828172
1111	1	0.022834027744309537
1111	2	-0.12297840967275064
1111	3	-0.08352985912984678
1111	4	0.09723219009181605
1111	5	0.20554536466297435
1111	6	0.22962798884395852
1111	7	0.14735371070847236
1111	8	0.16394663792405595
1111	9	0.13410668808799642
1111	10	0.14521996169141715
1111	11	0.14121804613635902
1111	12	0.2012423888261594
1111	13	0.04120954059923321
1111	14	-0.002081500519146573
1111	15	-0.10388760699812138
1111	16	0.13514986061601775
1111	17	0.23301509502734064
1111	18	0.10594016528991546
1111	19	-0.09007453600769111
1111	20	0.04503830629278838
1111	21	0.29059495560204596
1111	22	-0.02488273420342324
1111	23	0.14059043486183498
1111	24	0.23993815377730243
1111	25	0.19963237871818507
1112	1	-0.003554477055587313
1112	2	-0.48178315621199985
1112	3	-0.14829619375885272
1112	4	-0.07937435749460064
1112	5	-0.04163412188481175
1112	6	-0.16857556278673513
1112	7	-0.022926198292722688
1112	8	-0.1422424677176295
1112	9	0.06119226051472282
1112	10	-0.37202852537434694
1112	11	-0.28693048615780725
1112	12	0.09133695786599816
1112	13	0.14592263587472587
1112	14	-0.11450580248146025
1112	15	-0.3814503975926655
1112	16	-0.5132106970134487
1112	17	-0.27002370757583094
1112	18	-0.06577933320243545
1112	19	-0.16114615763791562
1112	20	-0.14945204063555195
1112	21	-0.15699255150403246
1112	22	0.01231234105372181
1112	23	0.13752894620065348
1112	24	0.10195148907333618
1112	25	0.019910142295792355
1113	1	-0.8279812940197182
1113	2	-0.7095859358146015
1113	3	-0.6790269496871488
1113	4	-2.2917010416386674
1113	5	-0.875427311428659
1113	6	-0.5228604935422538
1113	7	-0.6754077232062059
1113	8	-0.6359905107194024
1113	9	-0.33277660351559746
1113	10	-0.5979053117427924
1113	11	-0.6236266843114251
1113	12	-0.3425362511608583
1113	13	-0.4924644087458532
1113	14	-0.6158818920050493
1113	15	-0.7462993326786531
1113	16	-0.8935083040011307
1113	17	-0.905872435961868
1113	18	-0.8748688097194801
1113	19	-0.5263853667752798
1113	20	-0.2640186563435236
1113	21	-0.3819905561332888
1113	22	-0.4420395113666089
1113	23	-0.2797366386680906
1113	24	-0.16105843047741075
1113	25	-0.3698563686296339
1114	1	-0.9464280750564278
1114	2	-2.2174963495650495
1114	3	-1.4515510118853534
1114	4	-0.9894407684092883
1114	5	-0.8819466208679856
1114	6	-0.6583510151521139
1114	7	-0.7083005040041854
1114	8	-0.7422808630529261
1114	9	-0.9927980885899785
1114	10	-1.2567527134594272
1114	11	-1.178740240525272
1114	12	-1.0503099417106796
1114	13	-1.1338166663063234
1114	14	-1.2100386028354748
1114	15	-1.437006495455222
1114	16	-1.6295999736518354
1114	17	-1.783902319747149
1114	18	-1.4600506190231712
1114	19	-2.0947241798876846
1114	20	-1.175936361998005
1114	21	-1.5473735438607576
1114	22	-1.2552257044935544
1114	23	-1.0316675826007256
1114	24	-1.2326970471858516
1114	25	-1.744781767676912
1115	1	-0.9797422892886871
1115	2	-1.2189614189673854
1115	3	-0.7034850455240473
1115	4	-0.4679263171001793
1115	5	-0.8315781684772787
1115	6	-0.5888558140464895
1115	7	-0.537617187331783
1115	8	-0.7235321262792453
1115	9	-1.8156820137960565
1115	10	-1.42578850987924
1115	11	-1.816578580331793
1115	12	-2.3768893160244424
1115	13	-2.61005271619079
1115	14	-1.9020495498864918
1115	15	-2.0826159304584735
1115	16	-1.852310188168154
1115	17	-1.7172269129199904
1115	18	-2.133844886570408
1115	19	-2.454931321691326
1115	20	-2.463381667712426
1115	21	-1.811630111271974
1115	22	-1.7735106665011742
1115	23	-1.7806993366963155
1115	24	-2.513552271889914
1115	25	-2.2689936510176167
1116	1	-1.8136812618043763
1116	2	-1.9231683870862675
1116	3	-2.0174112134402584
1116	4	-1.7592265033392636
1116	5	-2.155800167160777
1116	6	-2.8545323855513764
1116	7	-2.3398264047354402
1116	8	-2.8162102909391855
1116	9	-3.7687815642570968
1116	10	-2.4784209256556053
1116	11	-3.5028974805778113
1116	12	-5.305269417592051
1116	13	-4.52406007883549
1116	14	-3.84486959332461
1116	15	-4.921981381995501
1116	16	-4.451497392896528
1116	17	-4.257456356926898
1116	18	-4.24001854789087
1116	19	-4.698641009197619
1116	20	-6.145225742034224
1116	21	-5.165008649364069
1116	22	-4.435007723457261
1116	23	-5.08291332402527
1116	24	-5.196149570416221
1116	25	-4.556082099212521
1201	1	0.6921866472008524
1201	2	0.8960829624239572
1201	3	0.9879219711317404
1201	4	0.7829679712656414
1201	5	0.11256233337213217
1201	6	0.9127198474560458
1201	7	1.2248890846757448
1201	8	1.019002741642268
1201	9	0.8844285511508545
1201	10	1.0646946900212981
1201	11	0.7453906302167255
1201	12	1.2508365095493659
1201	13	1.1791469978500686
1201	14	1.302383038798581
1201	15	1.2300177798613703
1201	16	1.0882629290084864
1201	17	0.9946523803524201
1201	18	1.2709891105966449
1201	19	1.3839777708324448
1201	20	1.4277589273845237
1201	21	1.1358686343265205
1201	22	1.235169347885595
1201	23	1.2155241377072343
1201	24	0.9245364642506146
1201	25	1.1422033810462153
1202	1	1.1908644931768784
1202	2	1.2183178208336187
1202	3	1.125587658493881
1202	4	1.0686986495129958
1202	5	1.2824015193043365
1202	6	1.1409688806947926
1202	7	1.292035090246813
1202	8	1.2549459122475213
1202	9	1.6494456378778466
1202	10	1.5697208264884068
1202	11	1.4513242493508898
1202	12	1.5679067909089426
1202	13	1.5566010662378602
1202	14	1.772219251510215
1202	15	1.678261442720455
1202	16	1.70951504510532
1202	17	1.5810235088940345
1202	18	1.5772545225642318
1202	19	1.8768959310778826
1202	20	1.5942462716853285
1202	21	1.866481377921552
1202	22	1.6542150555771258
1202	23	1.7244047759090293
1202	24	1.6060185961240947
1202	25	1.6956056745142334
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2211	6	-0.08476874460491501
2211	7	-0.1921652304048891
2211	8	-0.4292378883417811
2211	9	-0.008148535505178245
2211	10	-0.16728519656385288
2211	11	-0.034449990906786354
2211	12	-0.219799123092848
2211	13	-0.031492843546352844
2211	14	-0.16946537959588426
2211	15	0.06253705152537492
2211	16	-0.055499254227378
2211	17	-0.1986900350294718
2211	18	-0.17784683659456846
2211	19	-0.2651644186057993
2211	20	-0.2342199623904122
2211	21	-0.09984929053614167
2211	22	-0.14471502213035592
2211	23	-0.32200576659898417
2211	24	-0.1006717583260297
2211	25	-0.3091688943392157
2212	1	-0.12689978707228988
2212	2	-0.07674637970853126
2212	3	0.05205615824446497
2212	4	0.040158122310536726
2212	5	-0.2711619525132818
2212	6	-0.15511695435606043
2212	7	-0.12348612087382094
2212	8	-0.09873223826560462
2212	9	-0.05486218033334467
2212	10	-0.34866957103481866
2212	11	-0.04820221284685999
2212	12	0.0060637303014274835
2212	13	0.07952215983946331
2212	14	0.20924049902270525
2212	15	-0.07694259127130465
2212	16	0.14375586681545371
2212	17	-0.10159654425581266
2212	18	-0.09535466628815215
2212	19	-0.008564727561278573
2212	20	-0.05639333719996387
2212	21	0.10535745569713839
2212	22	0.12711289390146874
2212	23	-0.15059367078046648
2212	24	-0.273221275847618
2212	25	-0.1538288317016665
2213	1	-0.478176375810893
2213	2	-0.25541594408952406
2213	3	-0.220296276260175
2213	4	-0.5274908123201811
2213	5	-0.2786480033636636
2213	6	-0.31521177131219247
2213	7	-0.787941807248238
2213	8	-0.7747956794920015
2213	9	-0.4639886373143369
2213	10	-0.6119714142549526
2213	11	-0.3465456890468701
2213	12	-0.8138780727805113
2213	13	-0.6380665934934697
2213	14	-0.7034084486061403
2213	15	-0.9875932945129478
2213	16	-1.3072442519007126
2213	17	-1.1895177808450015
2213	18	-0.6899952812552286
2213	19	-0.7582035964475011
2213	20	-0.7957608746540501
2213	21	-0.5647294497363333
2213	22	-0.7244446708746608
2213	23	-0.8804935939182457
2213	24	-0.5023384954976535
2213	25	-0.5383994561227112
2214	1	-1.4475056767019971
2214	2	-0.8029318418540079
2214	3	-0.8686978065406663
2214	4	-1.0431871312052508
2214	5	-1.3515728544472623
2214	6	-2.0277074219185955
2214	7	-1.6110652907523146
2214	8	-1.3421873958636574
2214	9	-1.1936495269272243
2214	10	-1.2829555918395101
2214	11	-1.3889015567070402
2214	12	-1.2266804811255199
2214	13	-1.716669871651007
2214	14	-1.2895641715696797
2214	15	-1.3929472904721223
2214	16	-1.4158596145877382
2214	17	-1.4962068999805425
2214	18	-1.57974320066198
2214	19	-1.6695494405895062
2214	20	-1.751920752295156
2214	21	-1.946015881768524
2214	22	-2.390719699056085
2214	23	-2.3646086266360555
2214	24	-1.9709247864737947
2214	25	-2.562909626698371
2215	1	-1.5327128025486978
2215	2	-0.8210438912785136
2215	3	-1.015322779281565
2215	4	-1.0568116818051578
2215	5	-1.4313528342938682
2215	6	-1.2846732485046743
2215	7	-1.6702610621399572
2215	8	-1.4569577291549791
2215	9	-1.3740411110496495
2215	10	-1.3498252596418894
2215	11	-1.0701891067487495
2215	12	-1.1159128180593925
2215	13	-1.4462755599509052
2215	14	-1.5792082460238817
2215	15	-1.4642724464075414
2215	16	-1.6616877211753405
2215	17	-1.7728853105696842
2215	18	-1.2433901920904518
2215	19	-1.5338301103331986
2215	20	-1.472936905023495
2215	21	-1.1655045243084672
2215	22	-1.4195546632674692
2215	23	-1.8489884094835247
2215	24	-1.97102081854851
2215	25	-2.2627673791877
2216	1	-1.5416565076949347
2216	2	-0.9553190468727912
2216	3	-1.0066049444478047
2216	4	-1.2530569200224093
2216	5	-2.0577405606149703
2216	6	-2.3855632534586384
2216	7	-2.1498672506069063
2216	8	-2.2199761157576425
2216	9	-4.02650262293821
2216	10	-1.989998667303638
2216	11	-2.18248461344799
2216	12	-2.428088366743932
2216	13	-2.419968311791557
2216	14	-2.807041162730883
2216	15	-2.6815881580603573
2216	16	-1.885008925842314
2216	17	-2.241365013559715
2216	18	-2.5250447775425044
2216	19	-2.8178515888916564
2216	20	-2.160110318379452
2216	21	-2.6127068642489775
2216	22	-2.157993045276804
2216	23	-3.414480360718315
2216	24	-2.7693831638795423
2216	25	-2.936181684180955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43801.0
3	1.0
4	736.0
5	6336.0
6	7751.0
7	5885.0
8	4820.0
9	3977.0
10	3271.0
11	2994.0
12	2735.0
13	2558.0
14	2341.0
15	2471.0
16	2524.0
17	2542.0
18	2906.0
19	3457.0
20	4615.0
21	6127.0
22	7575.0
23	9264.0
24	10639.0
25	12030.0
26	13247.0
27	14723.0
28	16749.0
29	19634.0
30	24501.0
31	31359.0
32	39155.0
33	50823.0
34	65443.0
35	85710.0
36	117606.0
37	181439.0
38	559144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.782462239542454	19.34034822336582	13.495352811784315	26.381836725307412
2	14.03195874859249	20.603373350487363	38.965752020135305	26.398915880784834
3	17.190824167325726	25.280670983858684	29.59149129898063	27.937013549834965
4	11.978433377929447	16.764102122668003	36.208639779635874	35.04882471976668
5	13.270302273305964	38.57659922649668	33.99620763041104	14.156890869786313
6	32.29576926108777	36.12638204819031	17.627008294478813	13.950840396243105
7	28.545879000150375	31.824472985343306	21.311189398241897	18.31845861626442
8	26.524576931133215	35.20030769971835	20.205931101829194	18.069184267319237
9	27.301898603954804	14.32919933517488	19.63094617922553	38.73795588164479
10	16.284668805446007	28.691364800051378	32.46234999109653	22.56161640340609
11	34.76357842858331	22.416826790505496	23.528574992668588	19.291019788242604
12	23.64851902238335	24.94665854539221	30.1529304062704	21.251892025954035
13	29.01587741598976	21.591753810689262	25.718860677771982	23.673508095548996
14	21.875681586247698	21.71914014543911	27.16351431887331	29.24166394943989
15	24.12467088296383	29.604154917914983	23.11884398849358	23.152330210627607
16	23.06291071866282	27.393986436800393	26.38506297199818	23.158039872538605
17	21.992191603347454	27.874385774478565	27.018336309628022	23.11508631254596
18	22.124323756499816	26.488611096521176	29.052062725049755	22.335002421929257
19	23.673725444425802	26.329994820807222	27.55374324334182	22.44253649142515
20	24.432625562049378	25.88651344104062	27.643711028568596	22.03714996834141
21	24.149363367459706	25.805228009643404	26.987092279804653	23.058316343092237
22	24.094561019839784	26.72286892672017	26.962714515180735	22.21985553825931
23	23.197501767101496	26.37412657042255	27.56554323508322	22.86282842739274
24	23.660121278965693	25.883496037972442	27.806846506172274	22.649536176889594
25	23.265851575145764	26.270179423717018	27.868266504436175	22.595702496701044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	307.5
3	506.0
4	506.0
5	506.0
6	1187.0
7	1868.0
8	1868.0
9	1868.0
10	2123.0
11	2378.0
12	2378.0
13	2378.0
14	3387.5
15	4397.0
16	4397.0
17	4397.0
18	8766.0
19	13135.0
20	13135.0
21	13135.0
22	21286.0
23	29437.0
24	29437.0
25	29437.0
26	44863.5
27	60290.0
28	60290.0
29	60290.0
30	75321.0
31	90352.0
32	90352.0
33	90352.0
34	102533.0
35	114714.0
36	114714.0
37	114714.0
38	125162.5
39	135611.0
40	135611.0
41	135611.0
42	146779.0
43	157947.0
44	157947.0
45	157947.0
46	170413.0
47	182879.0
48	182879.0
49	182879.0
50	179969.5
51	177060.0
52	177060.0
53	177060.0
54	160242.0
55	143424.0
56	143424.0
57	143424.0
58	126202.5
59	108981.0
60	108981.0
61	108981.0
62	91567.0
63	74153.0
64	74153.0
65	74153.0
66	57168.5
67	40184.0
68	40184.0
69	40184.0
70	30254.0
71	20324.0
72	20324.0
73	20324.0
74	14448.5
75	8573.0
76	8573.0
77	8573.0
78	5883.5
79	3194.0
80	3194.0
81	3194.0
82	2110.0
83	1026.0
84	1026.0
85	1026.0
86	656.5
87	287.0
88	287.0
89	287.0
90	167.5
91	48.0
92	48.0
93	48.0
94	29.5
95	11.0
96	11.0
97	11.0
98	6.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08286593589998899
2	0.04084940502112133
3	0.017871614696740583
4	0.00488733952931273
5	0.017652778598413146
6	0.05988814557560824
7	0.07316420220747268
8	0.05266655433080286
9	0.022102445931071003
10	0.04646619821152551
11	0.005470902458185893
12	2.9178146443658095E-4
13	0.005397957092076747
14	4.3767219665487143E-4
15	0.01283838443520956
16	0.008826389299206573
17	0.004157885868221279
18	0.005908574654840764
19	0.0013859619560737594
20	9.48289759418888E-4
21	2.9178146443658095E-4
22	5.106175627640167E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1370889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.384349531855925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77236133393328	42.723753063065786
2	13.816221977925968	15.580373784261752
3	4.643856627435688	7.855225057714782
4	2.0993592941564754	4.7348403293867625
5	1.1098103508708828	3.1287967368787757
6	0.6209636532598752	2.1007579003189796
7	0.4335325492053851	1.7111115551483072
8	0.26921313069705854	1.2143525807830529
9	0.1967305568385299	0.9983272032342289
>10	0.9258646350514513	8.969427678861521
>50	0.06366373117940498	2.451598209284937
>100	0.04219094849915196	4.542085071988086
>500	0.004413774420719944	1.6763442201649417
>1k	0.0016876196314517433	1.8519424117716
>5k	1.298168947270572E-4	0.4610641971364495
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6299	0.4594828611215058	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4847	0.3535661895310269	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3678	0.2682930565494362	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2230	0.16266816642339388	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2068	0.15085101711371235	No Hit
GTCCTACAGTGGACATTTCTAAATT	1675	0.12218348823281827	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1580	0.11525367845244947	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1527	0.11138757404866477	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1512	0.1102933935570276	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1436	0.10474954573273255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.294536610914524E-5
2	0.0	0.0	0.0	0.0	7.294536610914524E-5
3	0.0	0.0	0.0	0.0	7.294536610914524E-5
4	0.0	0.0	0.0	0.0	7.294536610914524E-5
5	0.0	0.0	0.0	0.0	7.294536610914524E-5
6	7.294536610914524E-5	0.0	0.0	0.0	7.294536610914524E-5
7	7.294536610914524E-5	0.0	0.0	0.0	7.294536610914524E-5
8	7.294536610914524E-5	0.0	0.0	0.0	7.294536610914524E-5
9	7.294536610914524E-5	0.0	0.0	0.0	7.294536610914524E-5
10	7.294536610914524E-5	0.0	0.0	7.294536610914524E-5	7.294536610914524E-5
11	7.294536610914524E-5	0.0	0.0	1.4589073221829048E-4	7.294536610914524E-5
12	7.294536610914524E-5	0.0	0.0	1.4589073221829048E-4	1.4589073221829048E-4
13	7.294536610914524E-5	0.0	0.0	1.4589073221829048E-4	1.4589073221829048E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGACT	40	0.005299978	14.241354	13
GGTATCA	1175	0.0	14.163603	1
ATAGGAC	115	2.5465852E-11	14.058035	3
CTTATAC	150	0.0	13.947777	1
CCGTATG	50	0.0015004745	13.298722	9
GTTGGCG	50	0.0015062619	13.291931	13
CTATTAG	75	1.4626152E-5	12.679797	1
ACACCGA	60	4.0646314E-4	12.676554	6
GGCGAGG	485	0.0	11.939462	19
TAGGACT	145	9.640644E-11	11.802309	4
GGTTAGA	65	7.952928E-4	11.704428	1
TGTACCG	65	7.9700106E-4	11.701435	5
TGTAGGA	895	0.0	11.263059	2
GTAGGAC	855	0.0	11.011402	3
GTATCAA	2655	0.0	10.99632	1
TAATAGT	130	3.2263415E-8	10.970096	4
TAGGACG	1090	0.0	10.903	4
AGGACGT	1110	0.0	10.877854	5
GGACGTG	1085	0.0	10.865618	6
TCGGGCT	70	0.0014987757	10.850555	13
>>END_MODULE