FastQCFastQC Report
Thu 26 May 2016
SRR1512860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226413
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT85800.38537324386805144No Hit
CTGTAGGACGTGGAATATGGCAAGA70470.31651809435176675No Hit
GTCCTAAAGTGTGTATTTCTCATTT63810.2866045068906802No Hit
GTATCAACGCAGAGTACTTTTTTTT57930.2601943125556669No Hit
CTTTAGGACGTGAAATATGGCGAGG50580.22718156963690023No Hit
GTCCTACAGTGTGCATTTCTCATTT45350.20369086957361457No Hit
CTGTAGGACCTGGAATATGGCGAGA34390.1544637046226374No Hit
TATCAACGCAGAGTACTTTTTTTTT33550.1506908197176355No Hit
GGTATCAACGCAGAGTACTTTTTTT32660.146693358330193No Hit
CTGAAGGACCTGGAATATGGCGAGA26820.12046282518113217No Hit
GTACTGGTTCACTATCGGTCAGTCA25260.11345603892898577No Hit
GTCCTTCAGTGTGCATTTCTCATTT23440.10528145496814832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG551.9643351E-413.8152079
TAGGACC13800.013.4213244
CCAACGA1550.012.86935219
CGTGCGC1207.4396667E-1012.663938510
GGTATCA18900.012.35020351
TCGCATT550.003071010812.08993618
TCCAACG2850.011.99903518
GGACCGG802.8733299E-511.8727096
ACTGTTC6900.011.8382968
TAGAAAT8500.011.7330314
GTATTAG2200.011.6925951
GGCGAGG15850.011.62630219
GATATAC7300.011.6154751
AGGACCT24200.011.5390975
GTAAGAC1751.8189894E-1211.3978013
AATCCCG3850.011.34922619
GGACCTG23300.011.3325796
GGACCGT855.3354917E-511.1743156
ATTTAGA8400.011.1151831
TAAGACG600.00588545111.0811964
Produced by FastQC (version 0.11.5)