FastQCFastQC Report
Thu 26 May 2016
SRR1512860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226413
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80250.360445254317146No Hit
GTATCAACGCAGAGTACTTTTTTTT77940.3500698208283908No Hit
CTGTAGGACGTGGAATATGGCAAGA70640.31728165439206474No Hit
GTCCTAAAGTGTGTATTTCTCATTT59580.2676053364762063No Hit
CTTTAGGACGTGAAATATGGCGAGG55910.2511214226650671No Hit
TATCAACGCAGAGTACTTTTTTTTT52940.2377815796080961No Hit
GTCCTACAGTGTGCATTTCTCATTT43750.19650442213551572No Hit
GGTATCAACGCAGAGTACTTTTTTT43520.19547137031628903No Hit
CTGTAGGACCTGGAATATGGCGAGA34480.15486794229103046No Hit
CTGAAGGACCTGGAATATGGCGAGA28150.12643655961405184No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27760.12468486305101524No Hit
GTACTGGTTCACTATCGGTCAGTCA22780.10231704539993254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT801.2718192E-714.2658836
TAGGACC11700.014.0626664
ACACCGT1205.0931703E-1113.4733326
GGTATCA19000.012.2174471
ACCGCCT550.0030688912.0910528
TAGAAAT7500.011.6663224
ACTATAC1800.011.6253613
GGCGAGG13950.011.2941319
ATTTAGA7100.011.255511
AGGACCT23750.011.2525285
TCTTATA2050.011.13562
TGTAGGA39600.011.0729162
GTAGGAC38150.011.02006153
CACCTTT8200.010.99757514
GGACCTG23400.010.8111826
GACGTGG18950.010.73463257
CTGTAGG40250.010.7072151
TCCCGAA803.7295397E-410.7006172
TTGCGAG803.8038823E-410.67778518
TGGCAAG29400.010.62128818
Produced by FastQC (version 0.11.5)