FastQCFastQC Report
Thu 26 May 2016
SRR1512859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716157
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70150.408762135399034No Hit
GTCCTACAGTGGACATTTCTAAATT53410.31121861228314196No Hit
GTCCTAAAGTGTGTATTTCTCATTT49760.28995016190243666No Hit
CTGTAGGACGTGGAATATGGCAAGA44800.26104837727550567No Hit
CTTTAGGACGTGAAATATGGCGAGG42160.24566516932891339No Hit
GGTATCAACGCAGAGTACTTTTTTT40060.23342852664412403No Hit
TATCAACGCAGAGTACTTTTTTTTT39180.22830079066192663No Hit
GTCCTACAGTGTGCATTTCTCATTT25460.148354725121303No Hit
CTGAAGGACCTGGAATATGGCGAGA21620.12597914992625966No Hit
GTACTGGTTCACTATCGGTCAGTCA19500.11362596778732947No Hit
GTCCTTCAGTGTGCATTTCTCATTT18900.11012978416310396No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18420.10733283726372354No Hit
CTGTAGGACCTGGAATATGGCGAGA17360.10115624619425846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.014.0551111
TCGGTTT550.003070785412.08979513
TAGGACC6900.011.8391444
CTTAGAC1302.6211637E-911.6908893
CCCGTAC1406.82121E-1011.53397816
GTTGGAC752.0763896E-411.3986183
GTCCTAG2850.011.3574731
GTTAGAC700.001493048210.8558253
TAGGACA6950.010.7972514
GATATAC3450.010.7620051
GTATCAA35250.010.7490871
GACGTGG12500.010.7147017
CTGGTCG803.77533E-410.6862049
ACCGGGG1701.382432E-1010.61666915
TGTAGGA24600.010.5800172
CTGTAGG24400.010.5737041
GCGCCAC909.524355E-510.55458413
TCCGAAT909.527173E-510.554275517
TAGAAAT6500.010.5218014
TAATAGT1451.4459147E-810.4814864
Produced by FastQC (version 0.11.5)