##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512859_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1716157
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.8549649012299	31.0	30.0	34.0	22.0	34.0
2	28.937369366555625	31.0	30.0	34.0	20.0	34.0
3	29.05969791808092	31.0	30.0	34.0	22.0	34.0
4	32.47840319970725	35.0	33.0	37.0	24.0	37.0
5	32.67490037333414	35.0	33.0	37.0	25.0	37.0
6	32.62279674878231	36.0	33.0	37.0	25.0	37.0
7	32.52268061721626	36.0	33.0	37.0	24.0	37.0
8	32.44469357989974	36.0	33.0	37.0	23.0	37.0
9	33.74284345779553	38.0	34.0	39.0	22.0	39.0
10	33.59236130493888	38.0	34.0	39.0	19.0	39.0
11	33.66689003395377	38.0	34.0	39.0	21.0	39.0
12	33.57524923419011	38.0	34.0	39.0	18.0	39.0
13	33.54082406213418	38.0	34.0	39.0	18.0	39.0
14	34.36625961377659	39.0	34.0	41.0	17.0	41.0
15	34.565952299236024	39.0	34.0	41.0	18.0	41.0
16	34.502035070217936	39.0	34.0	41.0	17.0	41.0
17	34.27097520797923	39.0	34.0	41.0	17.0	41.0
18	34.27613790579766	39.0	34.0	41.0	17.0	41.0
19	34.40443793895314	39.0	34.0	41.0	18.0	41.0
20	34.400810648443006	39.0	34.0	41.0	18.0	41.0
21	34.28365353519521	39.0	34.0	41.0	16.0	41.0
22	34.29224773724082	39.0	34.0	41.0	16.0	41.0
23	33.95784301785909	39.0	34.0	41.0	10.0	41.0
24	34.036566001828504	39.0	34.0	41.0	2.0	41.0
25	33.749435512019005	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6945970724086301
1101	2	0.3892736751443131
1101	3	0.691822863099155
1101	4	0.7059658705898926
1101	5	0.668337917454096
1101	6	0.6370758837398114
1101	7	0.45685880748899166
1101	8	0.7725961472060803
1101	9	0.7008883130934649
1101	10	0.7305710964477896
1101	11	0.7239047397021423
1101	12	0.6221229031967539
1101	13	1.0558844041560391
1101	14	1.0254357846807594
1101	15	0.943510911259132
1101	16	0.8106443904248337
1101	17	0.2025736945555252
1101	18	0.631617897446624
1101	19	0.09156869044103644
1101	20	-0.4228063203257548
1101	21	0.5512525669010131
1101	22	0.645613006753976
1101	23	0.09446113096918651
1101	24	0.6475540587235429
1101	25	0.3176825749439658
1102	1	1.0860998394854349
1102	2	1.0398973437910968
1102	3	1.0915138792894794
1102	4	1.2762829961793756
1102	5	1.1081905934035419
1102	6	1.0405954961649613
1102	7	1.10496125613966
1102	8	1.0978826683738134
1102	9	1.4361928756375946
1102	10	1.4868131276288494
1102	11	1.2178282461665688
1102	12	1.50093271870319
1102	13	1.4584712156604738
1102	14	1.6309270618118603
1102	15	1.5539871727153738
1102	16	1.6401728323162175
1102	17	1.7200121576060354
1102	18	1.6136192787580583
1102	19	1.3503353562960996
1102	20	1.6396438328002745
1102	21	1.8107989670288092
1102	22	1.6315369621743159
1102	23	1.5469544990708712
1102	24	0.9821350361765795
1102	25	1.3905433386322201
1103	1	0.6034760240844861
1103	2	0.7284989701212901
1103	3	0.8332145899264418
1103	4	0.9562236174629817
1103	5	0.6646036878426713
1103	6	0.15562598229182356
1103	7	0.2538685304214354
1103	8	0.40056053148924775
1103	9	0.4888875242049835
1103	10	0.6096641935554743
1103	11	0.5930328053546674
1103	12	0.5125282241787019
1103	13	0.6434891119779635
1103	14	0.7517121483772442
1103	15	0.5253707867139568
1103	16	0.6707693253238176
1103	17	0.4043162759923149
1103	18	0.6932533530238132
1103	19	0.7414061298129511
1103	20	0.8240930408871492
1103	21	0.5831104347055032
1103	22	0.6996409437987836
1103	23	-0.05403383986673305
1103	24	0.1121470784776406
1103	25	0.37987456300199085
1104	1	0.9255252657653195
1104	2	0.8662630465261252
1104	3	0.9424521699773756
1104	4	1.0673178779810755
1104	5	0.8542935038257014
1104	6	0.8483242663350481
1104	7	1.0023050361125456
1104	8	0.8171006771066871
1104	9	1.0963022665764086
1104	10	1.1009629154875569
1104	11	0.7872099767376639
1104	12	1.109917005308759
1104	13	1.1664546294341847
1104	14	1.3089480903806177
1104	15	1.1432425256640002
1104	16	1.0320035590452221
1104	17	1.2844677783996872
1104	18	1.0888428306639284
1104	19	0.7071781857523902
1104	20	0.806085562622286
1104	21	0.8266003709366316
1104	22	0.5757398077856095
1104	23	0.9723363469879303
1104	24	0.8289145230186605
1104	25	0.46772010281993914
1105	1	0.4649925004706503
1105	2	0.6210825949964693
1105	3	0.6878028804074532
1105	4	0.8434174985251417
1105	5	0.5964268554828607
1105	6	0.7813231898101591
1105	7	0.7033059752087212
1105	8	0.8289623016667278
1105	9	0.39144110774699215
1105	10	0.5578813057392793
1105	11	0.5873458784608658
1105	12	0.6626445834331633
1105	13	0.7279226734419098
1105	14	0.8285217559860314
1105	15	0.582457044243597
1105	16	0.5649184983093676
1105	17	1.0282456936061735
1105	18	0.8734767740280276
1105	19	0.6169577653732361
1105	20	0.7780088774027476
1105	21	0.7573515715290995
1105	22	0.8215997679312892
1105	23	0.978341981564995
1105	24	1.0009462537805476
1105	25	1.1616327745103945
1106	1	0.41911192601864755
1106	2	0.4875018899871897
1106	3	0.5748570960330959
1106	4	0.6743595723243558
1106	5	0.4539407701396385
1106	6	0.5353876438746141
1106	7	0.5357460219344858
1106	8	0.452929868491438
1106	9	0.5210295087639665
1106	10	0.44720309506107014
1106	11	0.0593379075957543
1106	12	0.321896971256983
1106	13	0.39413582536934655
1106	14	0.744738343631191
1106	15	0.46856300177812926
1106	16	0.5937853986048367
1106	17	0.7025946250979587
1106	18	0.5451252313907773
1106	19	0.6810476437488333
1106	20	0.8483105334186831
1106	21	0.6664424806200131
1106	22	0.5390119853435067
1106	23	0.884107115901557
1106	24	0.5155274397903042
1106	25	0.7534420234624974
1107	1	0.8774363576060829
1107	2	0.9065113999388608
1107	3	0.9253671557620926
1107	4	1.0382614323286958
1107	5	0.8512800623497867
1107	6	1.0347530421902817
1107	7	0.7026425529894098
1107	8	0.8662672821926165
1107	9	0.9517924853098236
1107	10	1.2374096348128987
1107	11	1.1416171229883076
1107	12	1.044730698741752
1107	13	0.9366501513373962
1107	14	1.2511267039144727
1107	15	1.0828886398786466
1107	16	1.2121622590697356
1107	17	1.372774147535445
1107	18	1.430400492620926
1107	19	1.1972250374661115
1107	20	1.357150963815009
1107	21	1.2339958024216315
1107	22	1.2381465844157447
1107	23	1.5416685681935647
1107	24	1.3222102519556316
1107	25	1.6088820694133759
1108	1	0.8247478438496891
1108	2	0.6690561081267745
1108	3	0.7165025199084845
1108	4	0.879728342153058
1108	5	0.670285401118413
1108	6	0.7984898791022843
1108	7	0.7038188189271395
1108	8	0.788996692363142
1108	9	0.8454148923827987
1108	10	0.9201028240583611
1108	11	0.49713214329939603
1108	12	0.7067023839369568
1108	13	0.8243388008684889
1108	14	0.7693607079748119
1108	15	0.7648333206965319
1108	16	0.5457909359762283
1108	17	0.8377649780383507
1108	18	0.9337736200391618
1108	19	0.7989313086708236
1108	20	0.8471055182763862
1108	21	0.9659432121764908
1108	22	0.9703772390084069
1108	23	1.3159349344889577
1108	24	1.0982930417196712
1108	25	1.310529406333849
1109	1	0.8557885001454224
1109	2	0.8847182194744363
1109	3	0.9789898948727824
1109	4	0.9609629013295091
1109	5	0.8637819250007084
1109	6	1.1122606644015889
1109	7	0.9258074350465506
1109	8	0.9130326991121933
1109	9	1.0392927438740998
1109	10	1.0370658661987378
1109	11	1.0807503681089514
1109	12	1.0263977340958164
1109	13	1.1655946201633753
1109	14	1.2683845681880612
1109	15	1.0917150031316112
1109	16	0.8327362809820187
1109	17	1.2514818768878868
1109	18	1.1896591687533942
1109	19	1.1271071492847184
1109	20	1.075673876035303
1109	21	1.16723707589383
1109	22	1.0738819194735427
1109	23	1.2254115881109797
1109	24	1.0116389191118031
1109	25	0.7605048188007615
1110	1	0.37533120644959794
1110	2	0.4916959561602354
1110	3	0.404904543307282
1110	4	0.3614397444922943
1110	5	0.3473286435927534
1110	6	0.37775127371300243
1110	7	0.25125866974661903
1110	8	0.287712043614313
1110	9	0.44073419543908443
1110	10	0.28726189253197276
1110	11	0.30675753913488535
1110	12	0.2830968593713834
1110	13	0.36220158254089085
1110	14	0.37286433930754015
1110	15	0.34444765550671974
1110	16	0.4937270997426282
1110	17	0.47550220140221455
1110	18	-0.13583328628394753
1110	19	0.40297727581670983
1110	20	0.4874040025489208
1110	21	0.47964010472220764
1110	22	0.5493297607159064
1110	23	0.6492602312333062
1110	24	0.5361502513570429
1110	25	0.7804509986928778
1111	1	0.5989711945875946
1111	2	0.5490331007119487
1111	3	0.6540725554328866
1111	4	0.6039642098783062
1111	5	0.7938548882795615
1111	6	0.524856614910405
1111	7	0.46216347715378703
1111	8	0.7503549643036322
1111	9	0.6760302513223095
1111	10	0.6376152814409934
1111	11	0.49923916950162095
1111	12	0.1687520704162253
1111	13	0.5284014573308369
1111	14	0.6106810713250681
1111	15	0.5947483243920217
1111	16	0.7440689561464637
1111	17	0.23560549969869982
1111	18	0.8010756728250854
1111	19	0.6823027890131925
1111	20	0.6012957288001957
1111	21	0.746848249965872
1111	22	0.38456733032280965
1111	23	0.6207831809186359
1111	24	0.654267817009945
1111	25	0.7707760505891486
1112	1	0.09397459457046153
1112	2	0.16134661720371923
1112	3	0.14249833692661795
1112	4	0.16886277088710955
1112	5	0.053240136478308386
1112	6	-0.14664000149855383
1112	7	-0.12883365374714373
1112	8	0.07190688992067606
1112	9	-0.06869924646372993
1112	10	0.06240123586324842
1112	11	-0.14511429440938173
1112	12	0.09405106268879848
1112	13	0.09757022232803791
1112	14	0.0161097282630962
1112	15	-0.1833431748047758
1112	16	0.01267064313018551
1112	17	0.2960428390191012
1112	18	0.12482040232287517
1112	19	0.10844358108758456
1112	20	-0.1842541853806381
1112	21	-0.18203077092366726
1112	22	-0.2562888572758695
1112	23	-0.04771875384611235
1112	24	0.016773937507878145
1112	25	-0.03617353025588699
1113	1	-0.11125981863881051
1113	2	0.10865216828032587
1113	3	0.08038173012915806
1113	4	0.10389987329322992
1113	5	0.10106507440679735
1113	6	-0.34186790480950435
1113	7	-0.4217404069805184
1113	8	-0.23968333840748812
1113	9	-0.2831414525498488
1113	10	-0.23877917663548942
1113	11	-0.27462072636288326
1113	12	-0.4549806484777861
1113	13	-0.3841366957603185
1113	14	-0.10617212156498823
1113	15	-0.34778414314044426
1113	16	-0.4113338208000954
1113	17	-0.25969493719160397
1113	18	-0.41149539665842383
1113	19	-0.9069754442742592
1113	20	-0.5793195084970719
1113	21	-0.4218692076916781
1113	22	-0.9802555196382841
1113	23	-4.383439094501796
1113	24	-1.7545268445496944
1113	25	-1.0135063720573285
1114	1	-0.6129460562690419
1114	2	-0.7396912843729062
1114	3	-0.6208036082690427
1114	4	-0.5270872647055
1114	5	-0.5426682869594686
1114	6	-1.1538572390368635
1114	7	-1.077815550388845
1114	8	-0.905055660244475
1114	9	-1.2331100575611202
1114	10	-0.7239726748895876
1114	11	-1.074396273502245
1114	12	-1.9464774365289372
1114	13	-1.1390851710435115
1114	14	-2.779147286850371
1114	15	-1.809870219391911
1114	16	-1.7812804351133664
1114	17	-1.5908345042532872
1114	18	-1.527419474768024
1114	19	-1.6596987109441415
1114	20	-1.286204102791423
1114	21	-1.0538437962184446
1114	22	-1.3393400360844296
1114	23	-5.340943207103262
1114	24	-2.6493702531647045
1114	25	-2.56612323670657
1115	1	-1.5791753617423225
1115	2	-1.5119197410133864
1115	3	-1.1666975064237626
1115	4	-1.2929408799384987
1115	5	-1.196829512209593
1115	6	-1.3898560069329449
1115	7	-1.8624741093683532
1115	8	-2.159000111859598
1115	9	-3.4072684533685837
1115	10	-3.8071010802957375
1115	11	-2.736995945129081
1115	12	-3.082500497050262
1115	13	-2.4315163837415064
1115	14	-3.042725793457006
1115	15	-2.767185794121044
1115	16	-2.9074842868753734
1115	17	-2.6316297692768096
1115	18	-5.387720675139342
1115	19	-3.226678297811695
1115	20	-3.5506804783542805
1115	21	-2.968854553173262
1115	22	-2.465413970210271
1115	23	-3.2075052539063016
1115	24	-2.960466769286608
1115	25	-3.1792473979487283
1116	1	-1.6399530751064404
1116	2	-1.5356852233286773
1116	3	-2.006280105888898
1116	4	-1.6216959288200457
1116	5	-1.7930911678794281
1116	6	-4.225089894346638
1116	7	-2.7994849187532296
1116	8	-2.9881413794467626
1116	9	-4.135845952219405
1116	10	-3.213892842858904
1116	11	-2.757008234624088
1116	12	-2.56117316840421
1116	13	-3.8022468504737823
1116	14	-6.901842055554972
1116	15	-4.847945784074618
1116	16	-3.7604384255760657
1116	17	-4.875269797646904
1116	18	-3.583393761935085
1116	19	-3.2059235838109927
1116	20	-3.8707254661200707
1116	21	-4.844438422658193
1116	22	-4.384058461025532
1116	23	-3.4700850053046643
1116	24	-4.391970210503175
1116	25	-4.344403678307177
1201	1	0.5223367284844365
1201	2	0.5940348257421384
1201	3	0.8432048652483104
1201	4	0.7695714205044908
1201	5	0.24163391191247285
1201	6	0.6920637910359844
1201	7	0.903020817157234
1201	8	0.8214348332013373
1201	9	1.2303169513395602
1201	10	1.0049302719533344
1201	11	1.1380135355530285
1201	12	0.9572029933073765
1201	13	1.1343355611776502
1201	14	1.272695906584687
1201	15	1.268652930222622
1201	16	1.218482445882529
1201	17	1.4399223877442395
1201	18	1.148246623316723
1201	19	1.3445332530463148
1201	20	1.3916873106528769
1201	21	1.567220856486486
1201	22	1.3140782842645038
1201	23	1.8011609625298135
1201	24	1.6317475479052348
1201	25	1.7851671149327757
1202	1	1.0202973841843779
1202	2	0.900736227728359
1202	3	0.9680480092218424
1202	4	1.004295846277543
1202	5	0.8922672388151156
1202	6	1.160453738429112
1202	7	1.2173466169861982
1202	8	1.14644296754323
1202	9	1.503465848668334
1202	10	1.2251073270624175
1202	11	1.324722884719293
1202	12	1.464582641039982
1202	13	1.4675343299406265
1202	14	1.8509834044611182
1202	15	1.5350331912702089
1202	16	1.2716984623297378
1202	17	1.6574017207569938
1202	18	1.6241791932216714
1202	19	1.6811113009770224
1202	20	1.4528414369957616
1202	21	1.6530099452386366
1202	22	1.6298519969509115
1202	23	1.6847819972181028
1202	24	1.6056915951506454
1202	25	2.0087860764436627
1203	1	0.8052808050527176
1203	2	0.8013262556328122
1203	3	0.8411105244291157
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2211	16	-0.15896215753443954
2211	17	0.24358435100861442
2211	18	-0.17199333011839713
2211	19	-0.12525905087584732
2211	20	-0.1328340095436502
2211	21	-0.0296029148024175
2211	22	0.030148473897597228
2211	23	0.20548532967102062
2211	24	-0.10131528852145522
2211	25	0.4646527511792655
2212	1	0.16260434342411045
2212	2	0.2543968307951765
2212	3	-0.07658497466363201
2212	4	0.21255946766711276
2212	5	0.03720774471095467
2212	6	-0.025568882920424585
2212	7	0.011722886110106856
2212	8	0.0218810461382688
2212	9	0.19915664962402957
2212	10	-0.07286379457104175
2212	11	-0.2375764731695611
2212	12	0.2393858545843699
2212	13	0.22035170748585386
2212	14	0.07146487222107112
2212	15	-0.22633457821262226
2212	16	-0.22724944416913218
2212	17	0.1212413108253898
2212	18	0.3932344490652113
2212	19	0.04661616770244592
2212	20	0.21678114723091824
2212	21	0.3806144370279938
2212	22	0.473220748744815
2212	23	0.33947484456120947
2212	24	0.3484790522450254
2212	25	0.4521075171590496
2213	1	-0.4321470358172377
2213	2	-0.27169084035938695
2213	3	-0.6061404015852787
2213	4	-0.3718422417345977
2213	5	-0.4370672602914709
2213	6	-0.359845531578344
2213	7	-0.4380169875428095
2213	8	-0.3691052900821816
2213	9	-0.89600921665388
2213	10	-0.3798021646365015
2213	11	-0.5637456124657945
2213	12	-0.5127526841344334
2213	13	-0.6909141216311454
2213	14	-0.6728502236826515
2213	15	-0.5611150430098064
2213	16	-0.6124561874444368
2213	17	-0.6625143705055407
2213	18	-0.6488985155175868
2213	19	-0.4730844726085621
2213	20	-0.4237871287888666
2213	21	-0.6562766958079536
2213	22	-0.8348914057302181
2213	23	-0.6342945577198975
2213	24	-0.4197172859763967
2213	25	-0.18202549030171866
2214	1	-2.7836250360056525
2214	2	-1.4911074460899236
2214	3	-1.7018066652510484
2214	4	-3.189033295563256
2214	5	-1.6066570853786146
2214	6	-1.4038316964602906
2214	7	-1.382919886009013
2214	8	-1.1011715631687515
2214	9	-1.0213655808530433
2214	10	-1.1500507167492273
2214	11	-1.1870442608052656
2214	12	-1.0756407313819878
2214	13	-1.4559086149491094
2214	14	-1.3910275455734507
2214	15	-1.7008182205111453
2214	16	-1.6481467208860394
2214	17	-3.6954731522614885
2214	18	-3.5611260973776133
2214	19	-2.3470288382971063
2214	20	-2.1812441407802012
2214	21	-2.1376994945931216
2214	22	-3.0823817157642672
2214	23	-1.9449818948185609
2214	24	-3.1328659753636394
2214	25	-2.979283700977728
2215	1	-2.009136928252513
2215	2	-3.451850207729219
2215	3	-2.2319776576949124
2215	4	-3.816173096753186
2215	5	-2.4859423950223416
2215	6	-2.2569419438877922
2215	7	-2.5468102389011165
2215	8	-1.718089584312402
2215	9	-4.138780768093575
2215	10	-2.562255806219394
2215	11	-2.2039487536197484
2215	12	-2.1911608251784074
2215	13	-2.1893977119613197
2215	14	-1.8064411037776793
2215	15	-2.0016778330936162
2215	16	-2.1825368609887974
2215	17	-2.4451505861629173
2215	18	-2.1775139771220395
2215	19	-2.1472565651714177
2215	20	-2.0673739394617527
2215	21	-2.5778713043735415
2215	22	-2.635039609685254
2215	23	-2.4265797032292333
2215	24	-2.3822271845707945
2215	25	-3.152276364677558
2216	1	-3.198035040642882
2216	2	-3.4085826080390547
2216	3	-4.520230279220716
2216	4	-5.264579358986278
2216	5	-4.966362227833265
2216	6	-3.94868910025761
2216	7	-5.719010266108903
2216	8	-3.386547985549523
2216	9	-4.199899174716727
2216	10	-3.1531620198671533
2216	11	-3.6860599113110837
2216	12	-3.967192708262658
2216	13	-3.405091607699969
2216	14	-4.528748712796226
2216	15	-3.814741457670575
2216	16	-4.2684745995774875
2216	17	-3.7677522058591357
2216	18	-3.39208013785343
2216	19	-4.141109548727364
2216	20	-4.794758577056804
2216	21	-4.3218674064416085
2216	22	-3.949058838372256
2216	23	-4.212402502799463
2216	24	-4.580870126787563
2216	25	-4.617460947301776
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86700.0
3	0.0
4	787.0
5	4698.0
6	5856.0
7	5242.0
8	4683.0
9	4242.0
10	3771.0
11	3677.0
12	3482.0
13	3295.0
14	3207.0
15	3353.0
16	3419.0
17	3681.0
18	4218.0
19	5079.0
20	6652.0
21	8640.0
22	10789.0
23	12931.0
24	15236.0
25	16910.0
26	18935.0
27	20988.0
28	23313.0
29	27406.0
30	32765.0
31	40695.0
32	51245.0
33	65326.0
34	84458.0
35	111688.0
36	152405.0
37	238011.0
38	628374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00692740722724	18.87034382111292	12.742558650254807	24.380170121405033
2	14.515420324921823	20.32136700734623	42.66921214791297	22.49400051981897
3	19.225589519905128	26.044061386409794	28.984478573905243	25.74587051977984
4	12.023760335996746	16.732703213169938	38.40156786049165	32.84196859034167
5	12.594939622683293	39.26504071484225	35.03615179616762	13.103867866306828
6	30.981865386196656	36.73797387080728	18.91307783415666	13.367082908839405
7	28.173308895266008	31.95028490020183	21.95897973595924	17.917426468572923
8	25.443343805669528	36.95774161522856	20.707693492257743	16.891221086844173
9	28.057749471195535	14.421155211034826	20.139533229098447	37.381562088671195
10	16.40218429062753	28.572844972638045	33.613067017689765	21.411903719044656
11	34.04125650920547	22.260039295801505	25.296278562793095	18.40242563219993
12	24.59235928842228	24.66538349372995	31.368970545348496	19.373286672499272
13	29.516758022103755	22.486267525343624	25.651312071689382	22.34566238086324
14	21.107701315939877	23.14558853375697	27.576417125647644	28.1702930246555
15	22.931838182678128	32.33593767769153	23.23836428434378	21.493859855286555
16	21.40347296134836	28.018079227842012	28.850015309023576	21.728432501786052
17	21.11508191806502	29.179442820671504	28.011084559134463	21.694390702129013
18	20.96629594913274	26.84646671212414	31.33334072163054	20.85389661711258
19	23.55540416954404	26.509686630956157	28.011616024935144	21.923293174564662
20	24.283298834626088	26.829204278422942	28.631535726138008	20.25596116081297
21	23.28136344418364	26.369465381755624	27.28130512749486	23.067866046565875
22	23.76354397065823	28.55852955175407	27.194916519677836	20.483009957909864
23	21.966079511752138	28.167163301622182	28.509821007279506	21.356936179346167
24	23.288331951098034	26.551865317791645	29.211589313979708	20.94821341713062
25	22.481786136701373	27.492902250517314	28.85821297680896	21.16709863597235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	246.0
2	613.0
3	980.0
4	980.0
5	980.0
6	1818.5
7	2657.0
8	2657.0
9	2657.0
10	3095.0
11	3533.0
12	3533.0
13	3533.0
14	4961.5
15	6390.0
16	6390.0
17	6390.0
18	12476.0
19	18562.0
20	18562.0
21	18562.0
22	30440.0
23	42318.0
24	42318.0
25	42318.0
26	64201.0
27	86084.0
28	86084.0
29	86084.0
30	112519.5
31	138955.0
32	138955.0
33	138955.0
34	154978.0
35	171001.0
36	171001.0
37	171001.0
38	180644.5
39	190288.0
40	190288.0
41	190288.0
42	200917.0
43	211546.0
44	211546.0
45	211546.0
46	220862.5
47	230179.0
48	230179.0
49	230179.0
50	219568.5
51	208958.0
52	208958.0
53	208958.0
54	182353.5
55	155749.0
56	155749.0
57	155749.0
58	132463.0
59	109177.0
60	109177.0
61	109177.0
62	90028.0
63	70879.0
64	70879.0
65	70879.0
66	54382.0
67	37885.0
68	37885.0
69	37885.0
70	28081.5
71	18278.0
72	18278.0
73	18278.0
74	13033.5
75	7789.0
76	7789.0
77	7789.0
78	5458.5
79	3128.0
80	3128.0
81	3128.0
82	2132.5
83	1137.0
84	1137.0
85	1137.0
86	726.5
87	316.0
88	316.0
89	316.0
90	206.5
91	97.0
92	97.0
93	97.0
94	56.5
95	16.0
96	16.0
97	16.0
98	12.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.340819633634918
2	0.01019720223732444
3	0.008274301244000403
4	0.01095470868923997
5	0.011537405959944224
6	0.07982952608648276
7	0.07843105263679255
8	0.10482723899969526
9	0.08390840698141254
10	0.09031807695915933
11	0.0856564987935253
12	0.09655293775569485
13	0.08309263080242658
14	0.08676362360786338
15	0.08495726206868019
16	0.0863557355183704
17	0.09905853601972314
18	0.10103970674011759
19	0.09492138539772293
20	0.09323156331268061
21	0.08052876281132787
22	0.10144759482961059
23	0.09428041839994825
24	0.08991018886966635
25	0.0890944126906804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1716157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.01979554051613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41516131916924	44.14196670155103
2	13.145875875766096	14.991503092743724
3	4.279216440796789	7.320001395835442
4	1.8695651193326588	4.264088834161154
5	0.9947252473495415	2.8359515111430094
6	0.5544113213967472	1.8967452114833936
7	0.37949571271011345	1.514713756306318
8	0.2523342814875272	1.1510439310625473
9	0.1695430365582943	0.8700578351884973
>10	0.8353269739475616	8.43377381252928
>50	0.05560579718078834	2.182724902980665
>100	0.04080518426202584	4.620334818634246
>500	0.005048711845331458	1.960086580027601
>1k	0.002678908326094243	3.0910887906235107
>5k	2.0606987123801868E-4	0.7259188257297317
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7015	0.408762135399034	No Hit
GTCCTACAGTGGACATTTCTAAATT	5341	0.31121861228314196	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4976	0.28995016190243666	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4480	0.26104837727550567	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4216	0.24566516932891339	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4006	0.23342852664412403	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3918	0.22830079066192663	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2546	0.148354725121303	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2162	0.12597914992625966	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1950	0.11362596778732947	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1890	0.11012978416310396	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1842	0.10733283726372354	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1736	0.10115624619425846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.826972707042538E-5
2	0.0	0.0	0.0	0.0	5.826972707042538E-5
3	0.0	0.0	0.0	0.0	5.826972707042538E-5
4	0.0	0.0	0.0	0.0	5.826972707042538E-5
5	0.0	0.0	0.0	0.0	5.826972707042538E-5
6	0.0	0.0	0.0	0.0	5.826972707042538E-5
7	0.0	0.0	0.0	0.0	5.826972707042538E-5
8	0.0	0.0	0.0	0.0	5.826972707042538E-5
9	0.0	0.0	0.0	0.0	5.826972707042538E-5
10	0.0	0.0	0.0	0.0	5.826972707042538E-5
11	0.0	0.0	0.0	0.0	5.826972707042538E-5
12	0.0	0.0	0.0	0.0	1.1653945414085076E-4
13	0.0	0.0	0.0	0.0	1.1653945414085076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1585	0.0	14.055111	1
TCGGTTT	55	0.0030707854	12.089795	13
TAGGACC	690	0.0	11.839144	4
CTTAGAC	130	2.6211637E-9	11.690889	3
CCCGTAC	140	6.82121E-10	11.533978	16
GTTGGAC	75	2.0763896E-4	11.398618	3
GTCCTAG	285	0.0	11.357473	1
GTTAGAC	70	0.0014930482	10.855825	3
TAGGACA	695	0.0	10.797251	4
GATATAC	345	0.0	10.762005	1
GTATCAA	3525	0.0	10.749087	1
GACGTGG	1250	0.0	10.714701	7
CTGGTCG	80	3.77533E-4	10.686204	9
ACCGGGG	170	1.382432E-10	10.616669	15
TGTAGGA	2460	0.0	10.580017	2
CTGTAGG	2440	0.0	10.573704	1
GCGCCAC	90	9.524355E-5	10.554584	13
TCCGAAT	90	9.527173E-5	10.5542755	17
TAGAAAT	650	0.0	10.521801	4
TAATAGT	145	1.4459147E-8	10.481486	4
>>END_MODULE