FastQCFastQC Report
Thu 26 May 2016
SRR1512859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716157
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85750.4996629096288976No Hit
TATCAACGCAGAGTACTTTTTTTTT63040.3673323594519615No Hit
GTCCTACAGTGGACATTTCTAAATT50470.2940873125244369No Hit
GGTATCAACGCAGAGTACTTTTTTT49980.29123209589798604No Hit
GTCCTAAAGTGTGTATTTCTCATTT44350.2584262395573365No Hit
CTTTAGGACGTGAAATATGGCGAGG44280.2580183514678436No Hit
CTGTAGGACGTGGAATATGGCAAGA44060.2567364174722942No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29900.17422648394057186No Hit
GTCCTACAGTGTGCATTTCTCATTT24470.14258602214133087No Hit
CTGAAGGACCTGGAATATGGCGAGA21810.12708627474059775No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19050.11100383006916034No Hit
ATTTAGAAATGTCCACTGTAGGACG18430.10739110699079396No Hit
TTTCTAAATTTTCCACCTTTTTCAG18330.1068084097200897No Hit
GTCCTTCAGTGTGCATTTCTCATTT17540.10220510128152611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC400.00528074114.2494548
TAGGACC7350.013.9741324
GGTATCA15700.013.5163661
TATGTCG957.465678E-812.98965316
CGAATGG957.471317E-812.98889619
AAGGCCG550.003044167312.1038565
CACCTTT6900.011.96872814
GGCGAGG12150.011.64025519
GTCGAGG907.5330227E-611.60119319
TGTCGAG1001.948345E-611.39092718
TCGAACT2100.011.29986519
AATCCCG1354.791218E-911.24964219
ATGTCGA1105.020138E-711.21833717
GTATTAG1201.2459714E-711.1020081
AGGACCG1201.2565943E-711.0952025
GGACCTG14850.011.0791916
TGTACTG3100.011.0440715
GTATTAA2250.010.9962741
GGCGTGC1053.4678815E-610.8567278
GACGTGG13000.010.8209657
Produced by FastQC (version 0.11.5)