##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512859_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1716157
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18029818950131	31.0	30.0	34.0	26.0	34.0
2	30.212627399474524	31.0	30.0	34.0	26.0	34.0
3	30.387424344043115	31.0	30.0	34.0	26.0	34.0
4	33.9627219421067	37.0	35.0	37.0	30.0	37.0
5	33.5348624863576	37.0	35.0	37.0	28.0	37.0
6	33.39282245155892	37.0	35.0	37.0	27.0	37.0
7	33.224139166754554	37.0	35.0	37.0	26.0	37.0
8	33.169620844712924	37.0	35.0	37.0	26.0	37.0
9	34.47336286831566	39.0	35.0	39.0	26.0	39.0
10	34.403045875173426	39.0	35.0	39.0	26.0	39.0
11	34.362652134973665	39.0	35.0	39.0	26.0	39.0
12	34.23565734370457	39.0	35.0	39.0	25.0	39.0
13	34.22312469080626	39.0	35.0	39.0	25.0	39.0
14	35.28406725025741	40.0	35.0	41.0	25.0	41.0
15	35.28531130893036	40.0	35.0	41.0	25.0	41.0
16	35.298576994995216	40.0	36.0	41.0	25.0	41.0
17	35.2958960048527	40.0	36.0	41.0	25.0	41.0
18	35.22509945185668	40.0	36.0	41.0	25.0	41.0
19	35.2467693806569	40.0	35.0	41.0	25.0	41.0
20	35.224313975935765	40.0	35.0	41.0	25.0	41.0
21	35.1840297828229	40.0	35.0	41.0	25.0	41.0
22	35.15154499267841	40.0	35.0	41.0	25.0	41.0
23	34.99232471155028	40.0	35.0	41.0	24.0	41.0
24	34.94724666799133	40.0	35.0	41.0	23.0	41.0
25	34.88651387955764	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7477393687847389
1101	2	0.6501286469054364
1101	3	0.5945013753502444
1101	4	0.6451661375447912
1101	5	0.626631209825895
1101	6	0.7610546018080413
1101	7	0.7494355094346474
1101	8	0.5521409787155633
1101	9	0.8157463916195056
1101	10	0.5521356851682171
1101	11	0.7030205315667573
1101	12	0.7189466770695887
1101	13	0.8968797936481252
1101	14	1.0083513058462188
1101	15	0.6267384222273265
1101	16	0.9052530845520295
1101	17	0.8934135754897738
1101	18	0.8013989013681311
1101	19	0.6645538265745543
1101	20	0.664393836121782
1101	21	0.893463162278259
1101	22	0.7339210895480193
1101	23	0.681058578331438
1101	24	0.8046561412285982
1101	25	0.8698283350110714
1102	1	0.8720495296190371
1102	2	1.0047255753581368
1102	3	0.6656291157777119
1102	4	0.7431080072561258
1102	5	0.8830889595709834
1102	6	0.8599770779305302
1102	7	0.9327585789390795
1102	8	1.0843520806350853
1102	9	1.2749960710851767
1102	10	1.2364347586918782
1102	11	1.2615316310514686
1102	12	1.2153453796107598
1102	13	1.1624501309648494
1102	14	1.201449707361263
1102	15	1.4038031591753892
1102	16	1.34377121530391
1102	17	1.4463361834485227
1102	18	0.8431014716407148
1102	19	1.1917983537366794
1102	20	1.4076198575113281
1102	21	1.4554787605268587
1102	22	1.1394032509737997
1102	23	1.3900055391790431
1102	24	1.2121324745415407
1102	25	1.3771289163377176
1103	1	0.6150410631235204
1103	2	0.7154413502538439
1103	3	0.5107835376132677
1103	4	0.30823930695151347
1103	5	0.397558307318576
1103	6	0.35992636137183354
1103	7	0.5767368376724846
1103	8	0.5562879576151545
1103	9	0.6011483527395853
1103	10	0.5030385963792909
1103	11	0.7620923948197884
1103	12	0.7717570332385648
1103	13	0.8752689140728975
1103	14	0.8212096566677616
1103	15	0.7389203129851651
1103	16	0.812676489739566
1103	17	0.8029828045874723
1103	18	0.8886410511601
1103	19	0.8249636116260604
1103	20	0.7767153766745452
1103	21	1.06221680114448
1103	22	0.8013707595257387
1103	23	0.8444898771276783
1103	24	0.9774950532710704
1103	25	0.7538924333812176
1104	1	0.8772433704936304
1104	2	0.85280412766204
1104	3	0.7911885918849215
1104	4	0.6112346343811907
1104	5	0.804612560280809
1104	6	0.8024845306101298
1104	7	0.6686350159109793
1104	8	0.6792968618083464
1104	9	0.8617260647009317
1104	10	0.7992704639375958
1104	11	0.9842439578326534
1104	12	0.9009625660104206
1104	13	0.7581925514334102
1104	14	1.0352361186124668
1104	15	1.0496806485583576
1104	16	0.9400549367832483
1104	17	0.9315582101415956
1104	18	0.604356395649944
1104	19	1.026062978298647
1104	20	1.0315609133772625
1104	21	1.0388454534397127
1104	22	0.9562441351073332
1104	23	0.9784178239919541
1104	24	1.1253646710288194
1104	25	1.1092357564257753
1105	1	0.4911190891312529
1105	2	0.5468740788748505
1105	3	0.4689701871268319
1105	4	0.36443710504397586
1105	5	0.355927676675293
1105	6	0.34727178333388764
1105	7	0.4960871639980482
1105	8	0.6035385225579475
1105	9	0.7621009396789802
1105	10	0.44472119192800363
1105	11	0.6937578419497115
1105	12	0.7416704765595981
1105	13	0.765051770991036
1105	14	0.7901595021072794
1105	15	0.8323203486583708
1105	16	0.7960815674130544
1105	17	0.5709300082435576
1105	18	0.5961122001041588
1105	19	0.730374422292627
1105	20	0.7743539228369372
1105	21	0.6605530814965519
1105	22	0.7015414010395205
1105	23	0.8800448272062553
1105	24	0.9445252870148337
1105	25	0.8499261066399342
1106	1	-0.049449865723417474
1106	2	0.2412463732471437
1106	3	-1.3587079548370227
1106	4	-0.38986097782553486
1106	5	-0.2558293493674455
1106	6	-0.12045995582641922
1106	7	0.012386208868520043
1106	8	0.014718348023485817
1106	9	0.2329248247885758
1106	10	0.02245086965768195
1106	11	-0.019668942905646247
1106	12	0.23015395075736222
1106	13	0.13438455460953946
1106	14	0.21104179441814352
1106	15	0.24414608905554047
1106	16	0.31472377176954325
1106	17	0.16709574726643694
1106	18	0.39974031801799015
1106	19	0.2851122770304855
1106	20	0.45694857686016377
1106	21	0.27646732598222457
1106	22	0.25299470963567927
1106	23	0.30386809388666336
1106	24	0.18503927038596402
1106	25	0.2577732859156896
1107	1	0.6664233151240424
1107	2	0.8902294295471478
1107	3	0.6554570256533729
1107	4	0.5423654594936664
1107	5	0.6855339224276662
1107	6	0.7280661487668283
1107	7	0.7726190922890552
1107	8	0.6177402123611557
1107	9	0.7868357226427278
1107	10	0.7081169553393636
1107	11	0.7310053213839609
1107	12	0.8637224826525554
1107	13	0.7384317351001002
1107	14	0.9294357847291934
1107	15	0.895843937266406
1107	16	1.1143889836365943
1107	17	0.9146947504908667
1107	18	1.0818428182881377
1107	19	0.9992279301629239
1107	20	0.970693559918665
1107	21	0.9919218841847908
1107	22	0.9864995339007834
1107	23	1.0466615291137131
1107	24	1.0161758315542215
1107	25	1.105998174674795
1108	1	0.4476767010895628
1108	2	0.3952201578829495
1108	3	0.35454117827273635
1108	4	0.31164889441604515
1108	5	0.4940772158701279
1108	6	0.44025336621094624
1108	7	0.385551242312296
1108	8	0.37894464953201634
1108	9	0.5345883825496998
1108	10	0.49041405887523126
1108	11	0.451801192327558
1108	12	0.3935943946856071
1108	13	0.548557811988367
1108	14	0.5455013727801585
1108	15	0.5401909913722207
1108	16	0.5128515570053409
1108	17	0.49924501495577545
1108	18	0.6018334242922805
1108	19	0.5566169370368854
1108	20	0.5931798123945384
1108	21	0.5831001047404385
1108	22	0.5692759893315937
1108	23	0.7588417561774037
1108	24	0.7755704323456953
1108	25	0.8197829629079436
1109	1	0.4399308920124909
1109	2	0.3443195474643126
1109	3	0.5624618974654396
1109	4	0.42127542892840353
1109	5	0.055672667029277534
1109	6	0.5075937607304226
1109	7	0.5585061337590744
1109	8	0.49578549016063533
1109	9	0.6781118496568794
1109	10	0.31899478401605563
1109	11	0.651596247141562
1109	12	0.7936939226638628
1109	13	0.865017030546511
1109	14	0.8516338274404518
1109	15	0.8616531103758831
1109	16	0.7839401516290678
1109	17	0.8208838108439167
1109	18	0.8850980008714089
1109	19	0.8719439512269815
1109	20	0.8650663976131625
1109	21	0.9225862105757798
1109	22	0.8346593928939896
1109	23	0.93116650006953
1109	24	0.7992333564070222
1109	25	0.8914313287939919
1110	1	0.15840544022643854
1110	2	0.029393020090004995
1110	3	0.049573387581947514
1110	4	0.022874830524628464
1110	5	0.1304273239756455
1110	6	0.07332280475072395
1110	7	-0.03352017269260443
1110	8	4.5224933239040865E-4
1110	9	-0.13583753255660014
1110	10	-0.01828340560773256
1110	11	0.04849702913336529
1110	12	0.07063656561474829
1110	13	0.15757574885132186
1110	14	0.22507091700108361
1110	15	0.1829042027476362
1110	16	-0.039824265479268206
1110	17	0.07833737331156243
1110	18	0.04251321792339269
1110	19	0.1460712713970267
1110	20	0.19896570105509426
1110	21	0.26483604546469763
1110	22	0.1456663125283768
1110	23	0.27655104971568534
1110	24	0.3525361753136096
1110	25	0.2986950873614873
1111	1	0.26946758395440185
1111	2	0.1861238843688966
1111	3	0.12368620843271572
1111	4	0.3602088448249319
1111	5	0.45691666464584557
1111	6	0.45644498340571005
1111	7	0.3489718369288042
1111	8	0.3552786027542467
1111	9	0.32993563954133265
1111	10	0.3328937336719804
1111	11	0.23143963020753233
1111	12	0.5339426053524647
1111	13	0.4382563314075796
1111	14	0.21019810405832118
1111	15	0.3508298345641734
1111	16	0.28682158776136646
1111	17	0.35504336032925465
1111	18	0.29568877560239315
1111	19	0.11029991154991592
1111	20	0.41263419095592013
1111	21	0.5147454329239238
1111	22	0.2624905900993042
1111	23	0.3005263042545536
1111	24	0.4553113903124597
1111	25	0.37459544651665055
1112	1	-0.09473469440425575
1112	2	-0.45182407527124724
1112	3	-0.07751055381065797
1112	4	-0.04856824003081073
1112	5	0.010058317555717622
1112	6	-0.07483631309289507
1112	7	-0.052599716583124234
1112	8	0.02472399310718032
1112	9	-0.07229634610154534
1112	10	-0.3285698928565637
1112	11	-0.2666495318315256
1112	12	0.14367282226397293
1112	13	-0.04411280097812664
1112	14	-0.19303893872858424
1112	15	-0.38500775623863603
1112	16	-0.42752895734630414
1112	17	-0.27391830711117393
1112	18	-0.10366921985654187
1112	19	-0.17129573795256903
1112	20	-0.16211321622666475
1112	21	-0.1713927893344902
1112	22	-0.06703860480801893
1112	23	0.21876266492981244
1112	24	0.15358789510892024
1112	25	0.18034432579134574
1113	1	-0.756376272649824
1113	2	-0.6382836205685649
1113	3	-0.4961504065651887
1113	4	-1.9813884979244847
1113	5	-0.748710542248638
1113	6	-0.3851957491336435
1113	7	-0.5013154334045495
1113	8	-0.31445776686691573
1113	9	-0.23344637015404146
1113	10	-0.4020320548252414
1113	11	-0.5374109892191186
1113	12	-0.09432210229012128
1113	13	-0.4876383959848454
1113	14	-0.5504712956663766
1113	15	-0.6159171281505351
1113	16	-0.9111540541082803
1113	17	-0.8524466151330685
1113	18	-0.6261512507307216
1113	19	-0.5115550767797288
1113	20	-0.39410618848031476
1113	21	-0.6132873637724714
1113	22	-0.6594975599994441
1113	23	-0.5537214065599798
1113	24	-0.4499088502764437
1113	25	-0.6821703683136846
1114	1	-1.0460146478384047
1114	2	-2.3079509473349944
1114	3	-1.5077621485750932
1114	4	-1.0432920286532195
1114	5	-0.7816231448505917
1114	6	-0.6568169522577989
1114	7	-0.5884476077277156
1114	8	-0.6341986800775317
1114	9	-0.9768011448914464
1114	10	-1.1469371169475409
1114	11	-1.0246404738014832
1114	12	-1.0028933603910204
1114	13	-1.0742524819644927
1114	14	-0.986684207672333
1114	15	-1.326816871592392
1114	16	-1.564669724611484
1114	17	-1.5778829486529702
1114	18	-1.3124557906449752
1114	19	-1.8360816659201902
1114	20	-1.1682416898199293
1114	21	-1.5066199773413516
1114	22	-1.1339191677069849
1114	23	-0.9525471487460351
1114	24	-0.9544402410034181
1114	25	-1.708020092861723
1115	1	-0.9630988733573851
1115	2	-1.0761480118164144
1115	3	-0.703017223958323
1115	4	-0.36529782168343417
1115	5	-0.6905701863639209
1115	6	-0.6566971800335679
1115	7	-0.49258431489899834
1115	8	-0.7282620077388273
1115	9	-1.5007985668295518
1115	10	-1.2971669950729634
1115	11	-1.5714130773565458
1115	12	-2.2115086751157946
1115	13	-2.210901598947295
1115	14	-1.6986479310610605
1115	15	-1.9350716578313794
1115	16	-1.8409595869213362
1115	17	-1.7728164708412706
1115	18	-2.0410010625730095
1115	19	-2.488019214146121
1115	20	-2.7057764730829277
1115	21	-1.9269241029731177
1115	22	-1.8162430701284151
1115	23	-1.8692930529586889
1115	24	-2.4129953877990715
1115	25	-2.3087543106870996
1116	1	-1.6365979516452605
1116	2	-1.836073200700266
1116	3	-1.9119354798609933
1116	4	-1.5102648943667916
1116	5	-1.9846856033616547
1116	6	-2.7913540298474118
1116	7	-2.4971541746755435
1116	8	-2.6098784712391847
1116	9	-3.7428589685917792
1116	10	-2.5134289350755843
1116	11	-3.416731301034833
1116	12	-5.404749136935429
1116	13	-4.431205803439436
1116	14	-3.743379797342506
1116	15	-4.850853865233191
1116	16	-4.446975436894718
1116	17	-4.188432027635955
1116	18	-4.035873594603888
1116	19	-4.165352046211279
1116	20	-6.042623949076553
1116	21	-5.094791022590659
1116	22	-4.1712941631510425
1116	23	-4.995606315029804
1116	24	-4.939086870294439
1116	25	-4.264467899109068
1201	1	0.3955629781237988
1201	2	0.7179118781587874
1201	3	0.7265066327901408
1201	4	0.6175574734259328
1201	5	-0.14522668536277905
1201	6	0.5054401533211816
1201	7	0.6605036637357671
1201	8	0.7396800172789995
1201	9	0.7326698123893536
1201	10	0.7638968387263176
1201	11	0.5393014552894755
1201	12	0.8712420705931052
1201	13	0.9134719601810843
1201	14	0.9558898411637173
1201	15	0.9261317148470738
1201	16	0.9151508960242651
1201	17	0.6667304398531471
1201	18	1.0624948822092577
1201	19	1.0969869910738055
1201	20	1.0872201837107909
1201	21	0.7399826245747789
1201	22	0.8355283682604195
1201	23	0.8399734320792902
1201	24	0.6916189631949834
1201	25	0.8338847340791844
1202	1	0.8695420102378932
1202	2	0.9470725580457966
1202	3	0.930173421351963
1202	4	0.7561755855099648
1202	5	0.812503530909936
1202	6	0.8400679747860522
1202	7	0.9245773709512903
1202	8	0.7051839289616453
1202	9	1.049736813451858
1202	10	1.0866701166213488
1202	11	1.1030676411901368
1202	12	1.1340949066710024
1202	13	1.2040132322481867
1202	14	1.3761247724753218
1202	15	1.1995648102449081
1202	16	1.125982384133728
1202	17	1.1731784534900527
1202	18	1.0737499319799184
1202	19	1.2641403745281252
1202	20	1.195969712206903
1202	21	1.2752819251653449
1202	22	1.1340735448116348
1202	23	1.3965490037147674
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2211	8	-0.4160117690119378
2211	9	-0.1879960428337455
2211	10	-0.20596626022901887
2211	11	-0.04317787022693409
2211	12	-0.1327670075791758
2211	13	0.0024045252598270395
2211	14	-0.1069184372580807
2211	15	-0.06568986752446193
2211	16	-0.13028834083215202
2211	17	-0.3202787776460241
2211	18	-0.377281883582242
2211	19	-0.14058911712307065
2211	20	-0.1170808444084841
2211	21	-0.07576470858834483
2211	22	-0.10956141978589784
2211	23	-0.18783352733414915
2211	24	-0.06064863495797823
2211	25	-0.3251219005682344
2212	1	-0.08794173961901564
2212	2	-0.11382988245022929
2212	3	-0.03251356031964647
2212	4	-0.00553757176299996
2212	5	-0.3851733203263237
2212	6	-0.028034208121169968
2212	7	-0.19623941901176778
2212	8	0.07963744089288838
2212	9	-0.043932778155983954
2212	10	-0.28088198336460835
2212	11	0.06843906135142674
2212	12	0.15191254642899565
2212	13	0.18197453077094394
2212	14	0.2510959431679396
2212	15	0.10342633032335868
2212	16	0.0946755889884443
2212	17	-0.06726064858064262
2212	18	-0.0767004255034962
2212	19	0.12764988664883958
2212	20	0.054581907412753594
2212	21	0.11558335143364928
2212	22	0.10273192893811967
2212	23	-0.11043177693494499
2212	24	-0.14662814202679186
2212	25	-0.04486564405243598
2213	1	-0.7397172448644227
2213	2	-0.32733179766619
2213	3	-0.3390517360049792
2213	4	-0.5986345760222065
2213	5	-0.3435245194801979
2213	6	-0.3860366664580752
2213	7	-0.8166479031326261
2213	8	-1.0344644085768522
2213	9	-0.7564520732421656
2213	10	-0.8077396713845033
2213	11	-0.5242899070046292
2213	12	-0.9058565842916337
2213	13	-0.6931076520035475
2213	14	-0.5862661326974816
2213	15	-0.9259004140953593
2213	16	-1.492809761038714
2213	17	-1.2479180393132836
2213	18	-0.8046968809247019
2213	19	-0.9693865310397527
2213	20	-0.8185493277586176
2213	21	-0.6430136814108707
2213	22	-0.8894750001417222
2213	23	-1.0552960681621144
2213	24	-0.832597041206391
2213	25	-0.9492638517533223
2214	1	-1.4182254275023354
2214	2	-0.6481978047273067
2214	3	-0.7994232519911826
2214	4	-0.9361983907285278
2214	5	-1.1813409574307698
2214	6	-1.9432949444255172
2214	7	-1.2423413413209623
2214	8	-0.9694917288975162
2214	9	-0.8642347196795299
2214	10	-1.1627555185163203
2214	11	-1.3438437311766123
2214	12	-0.972336311626762
2214	13	-1.3129873349263264
2214	14	-1.15320119871037
2214	15	-1.3637436003894408
2214	16	-1.3080274936764056
2214	17	-1.36359901737179
2214	18	-1.4636594265146456
2214	19	-1.27987087232394
2214	20	-1.5780598498952543
2214	21	-1.8122740588709334
2214	22	-2.162995037286592
2214	23	-2.156084331190854
2214	24	-1.784128145548486
2214	25	-2.3095149721556325
2215	1	-1.7087195215411342
2215	2	-1.111933709194755
2215	3	-1.223080353941235
2215	4	-1.2378871771528495
2215	5	-1.7888226871403923
2215	6	-1.707444026612933
2215	7	-2.0668546737711964
2215	8	-1.8395924303977544
2215	9	-1.7779718924278427
2215	10	-1.6354824069160614
2215	11	-1.5339990533058554
2215	12	-1.6003548905321523
2215	13	-1.823080595845525
2215	14	-1.9330840095589679
2215	15	-1.7934481473172426
2215	16	-1.9088828786560157
2215	17	-2.1407665798205286
2215	18	-1.7037664130511416
2215	19	-1.9472890188864795
2215	20	-2.0572011392013962
2215	21	-1.8764885279826586
2215	22	-2.2016167260166455
2215	23	-2.5435483613364838
2215	24	-2.4909272583155655
2215	25	-2.6118232433197193
2216	1	-1.742758406466077
2216	2	-1.0655441547639093
2216	3	-1.1393547750122757
2216	4	-1.3700878706737853
2216	5	-2.2427604590447423
2216	6	-2.5620003592253546
2216	7	-2.4428471333273762
2216	8	-2.49036577009808
2216	9	-4.427184393573068
2216	10	-2.3919263491578135
2216	11	-2.6243394560366298
2216	12	-3.056747748765698
2216	13	-2.92324495308932
2216	14	-3.1461306986766857
2216	15	-3.199299764931226
2216	16	-2.544398202470319
2216	17	-2.726895605809517
2216	18	-3.022741345795154
2216	19	-3.2217203622570807
2216	20	-2.6534289738779933
2216	21	-3.415691339824157
2216	22	-2.7318731732904524
2216	23	-3.9547510288101257
2216	24	-3.333013540315946
2216	25	-3.507340343969094
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50286.0
3	3.0
4	853.0
5	7290.0
6	8980.0
7	6936.0
8	5525.0
9	4850.0
10	4038.0
11	3584.0
12	3251.0
13	3002.0
14	2883.0
15	2917.0
16	2985.0
17	2979.0
18	3424.0
19	4181.0
20	5660.0
21	7227.0
22	9230.0
23	11246.0
24	13077.0
25	14927.0
26	16567.0
27	17993.0
28	20212.0
29	23885.0
30	30030.0
31	38284.0
32	47787.0
33	62021.0
34	80490.0
35	105866.0
36	145119.0
37	226385.0
38	722184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.147047931420396	19.788718471503998	13.809290251994689	26.254943345080918
2	13.115874104024138	20.76874379170922	41.47936865471877	24.63601344954787
3	17.10155772826244	26.12050597028147	29.961907692594615	26.816028608861476
4	11.271090536777981	17.283080835451564	37.71448917108669	33.73133945668376
5	12.261153364221816	39.41760643412886	35.05813445231227	13.263105749337061
6	31.024102181073342	37.395142947268134	18.37531113835971	13.205443733298816
7	27.772020906675127	32.20328901676464	21.973410058786175	18.051280017774058
8	25.291717411747282	37.379647438046305	20.3804292377948	16.94820591241161
9	27.359748033992563	14.405983959547427	20.081105638403834	38.15316236805618
10	15.831948273479899	29.226987942254812	33.54889936566105	21.392164418604246
11	33.833746977792565	22.31978494761507	25.17390257959135	18.672565495001013
12	24.120898170619785	24.943143247550484	31.4119062672078	19.524052314621933
13	29.233310801361238	22.412241853526645	25.985560113747614	22.368887231364507
14	20.778321759002978	23.07141433694179	27.6896478987317	28.46061600532353
15	22.701021819881372	32.336630616833276	23.263040237581574	21.699307325703778
16	21.300155883509856	28.096443816377963	28.627205314607888	21.97619498550429
17	20.768054579506344	29.216503447930254	28.23637522711354	21.779066745449864
18	20.760793810996717	26.856153223983085	31.54714593059981	20.835907034420387
19	23.309941677218454	26.41732400464065	28.390357657509096	21.882376660631802
20	24.080615424776884	26.75689975456662	28.797691108226097	20.364793712430394
21	22.83415794708759	26.245267769790292	27.78457914980972	23.135995133312395
22	23.583445065795416	28.32684498410107	27.568812337827687	20.520897612275828
23	21.6039091994497	27.996039989348294	28.934707022725775	21.46534378847623
24	23.105520066054563	26.44851257781194	29.4353022479878	21.0106651081457
25	22.147216134654347	27.410196153382238	29.202514688341452	21.240073023621964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	125.0
2	411.5
3	698.0
4	698.0
5	698.0
6	1545.5
7	2393.0
8	2393.0
9	2393.0
10	2837.0
11	3281.0
12	3281.0
13	3281.0
14	4735.5
15	6190.0
16	6190.0
17	6190.0
18	12533.0
19	18876.0
20	18876.0
21	18876.0
22	31651.0
23	44426.0
24	44426.0
25	44426.0
26	67204.5
27	89983.0
28	89983.0
29	89983.0
30	115505.0
31	141027.0
32	141027.0
33	141027.0
34	157127.0
35	173227.0
36	173227.0
37	173227.0
38	182611.5
39	191996.0
40	191996.0
41	191996.0
42	201860.5
43	211725.0
44	211725.0
45	211725.0
46	221278.0
47	230831.0
48	230831.0
49	230831.0
50	218871.0
51	206911.0
52	206911.0
53	206911.0
54	180083.5
55	153256.0
56	153256.0
57	153256.0
58	130243.5
59	107231.0
60	107231.0
61	107231.0
62	88104.0
63	68977.0
64	68977.0
65	68977.0
66	52613.0
67	36249.0
68	36249.0
69	36249.0
70	26810.0
71	17371.0
72	17371.0
73	17371.0
74	12364.5
75	7358.0
76	7358.0
77	7358.0
78	5049.0
79	2740.0
80	2740.0
81	2740.0
82	1853.5
83	967.0
84	967.0
85	967.0
86	603.0
87	239.0
88	239.0
89	239.0
90	155.0
91	71.0
92	71.0
93	71.0
94	39.5
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08612265661008871
2	0.04131323649293159
3	0.018937661297888247
4	0.006001781888253814
5	0.01788880621062059
6	0.0631643841443411
7	0.07563410573741214
8	0.05151043873025603
9	0.02155979901605739
10	0.04824733401431221
11	0.006409669977746791
12	1.1653945414085076E-4
13	0.004486768984422754
14	1.7480918121127613E-4
15	0.01159567568701465
16	0.0076916039732961485
17	0.0032048349888733957
18	0.004719847892704456
19	0.0010488550872676569
20	5.244275436338284E-4
21	0.0
22	2.330789082817015E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1716157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.829263744980096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42742375242572	42.668867980235326
2	13.735122803137365	15.336435870920925
3	4.425707470944908	7.412519689605364
4	1.9744961607023572	4.409386676772101
5	1.0282861903005058	2.8704230461803872
6	0.6143997530712975	2.058089151544087
7	0.39395124379954577	1.5395805524923458
8	0.2577651454571609	1.1512670631992796
9	0.1813904521420237	0.9114205854112408
>10	0.851797773217084	8.424230113909848
>50	0.059934813160076955	2.3455078730146233
>100	0.04103623304310267	4.5348648752379805
>500	0.00575724666189014	2.1915778200502256
>1k	0.0026169303008591544	2.981342963917289
>5k	3.140316361030985E-4	1.1644857375088875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8575	0.4996629096288976	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6304	0.3673323594519615	No Hit
GTCCTACAGTGGACATTTCTAAATT	5047	0.2940873125244369	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4998	0.29123209589798604	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4435	0.2584262395573365	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4428	0.2580183514678436	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4406	0.2567364174722942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2990	0.17422648394057186	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2447	0.14258602214133087	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2181	0.12708627474059775	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1905	0.11100383006916034	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1843	0.10739110699079396	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1833	0.1068084097200897	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1754	0.10220510128152611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1653945414085076E-4
2	0.0	0.0	0.0	0.0	1.1653945414085076E-4
3	0.0	0.0	0.0	0.0	1.1653945414085076E-4
4	0.0	0.0	0.0	0.0	1.1653945414085076E-4
5	0.0	0.0	0.0	0.0	1.1653945414085076E-4
6	0.0	0.0	0.0	0.0	1.1653945414085076E-4
7	0.0	0.0	0.0	0.0	1.1653945414085076E-4
8	0.0	0.0	0.0	0.0	1.1653945414085076E-4
9	0.0	0.0	0.0	0.0	1.1653945414085076E-4
10	0.0	0.0	0.0	0.0	1.1653945414085076E-4
11	0.0	0.0	0.0	0.0	1.1653945414085076E-4
12	0.0	0.0	0.0	0.0	1.7480918121127613E-4
13	0.0	0.0	0.0	0.0	1.7480918121127613E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGACC	40	0.005280741	14.249454	8
TAGGACC	735	0.0	13.974132	4
GGTATCA	1570	0.0	13.516366	1
TATGTCG	95	7.465678E-8	12.989653	16
CGAATGG	95	7.471317E-8	12.988896	19
AAGGCCG	55	0.0030441673	12.103856	5
CACCTTT	690	0.0	11.968728	14
GGCGAGG	1215	0.0	11.640255	19
GTCGAGG	90	7.5330227E-6	11.601193	19
TGTCGAG	100	1.948345E-6	11.390927	18
TCGAACT	210	0.0	11.299865	19
AATCCCG	135	4.791218E-9	11.249642	19
ATGTCGA	110	5.020138E-7	11.218337	17
GTATTAG	120	1.2459714E-7	11.102008	1
AGGACCG	120	1.2565943E-7	11.095202	5
GGACCTG	1485	0.0	11.079191	6
TGTACTG	310	0.0	11.044071	5
GTATTAA	225	0.0	10.996274	1
GGCGTGC	105	3.4678815E-6	10.856727	8
GACGTGG	1300	0.0	10.820965	7
>>END_MODULE