FastQCFastQC Report
Thu 26 May 2016
SRR1512858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106120.5088253288760771No Hit
GTCCTACAGTGGACATTTCTAAATT76290.3657961208062187No Hit
CTGTAGGACGTGGAATATGGCAAGA67540.3238415257471754No Hit
GTCCTAAAGTGTGTATTTCTCATTT66500.3188549224487291No Hit
TATCAACGCAGAGTACTTTTTTTTT62170.2980933914080825No Hit
CTTTAGGACGTGAAATATGGCGAGG59980.287592755616162No Hit
GGTATCAACGCAGAGTACTTTTTTT58790.2818869306881321No Hit
GTCCTACAGTGTGCATTTCTCATTT39860.19112116103468182No Hit
CTGAAGGACCTGGAATATGGCGAGA29020.13914541127010704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28870.13842618964052344No Hit
CTGTAGGACCTGGAATATGGCGAGA27340.13109012901877073No Hit
GTCCTTCAGTGTGCATTTCTCATTT23810.11416444666923668No Hit
TTTCTAAATTTTCCACCTTTTTCAG23800.11411649856059777No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23630.11330138071373637No Hit
GAATATGGCAAGAAAACTGAAAATC22720.10893810282759586No Hit
ATTTAGAAATGTCCACTGTAGGACG22690.10879425850167915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA601.4695433E-615.8311149
GGTATCA18700.014.1539691
GCACCGT701.0924288E-412.2128666
TAGGACC11400.011.8319584
TAAGACG658.033266E-411.6909484
AAATGTC8700.011.4641857
CACCTTT6700.011.34196514
AATGTCC9050.011.1257798
TCGTACA600.005882933711.08177959
GTATCAA44600.011.0578311
TAGAAAT10300.010.9744524
GTCGAGG1658.0035534E-1110.93812319
GGCGAGG17500.010.91016119
AGGACGG1053.472287E-610.8558815
TGTAGGA39050.010.8246072
ATTTAGA10300.010.8149291
CAATAGT1502.1373125E-910.7654154
GTCCTAC36300.010.6224191
CTGTAGG38650.010.6170231
CAAGTCG2150.010.6034186
Produced by FastQC (version 0.11.5)