##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512858_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2085588
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.66448694564794	31.0	30.0	34.0	18.0	34.0
2	28.75624092582044	31.0	30.0	34.0	16.0	34.0
3	28.88017000481399	31.0	30.0	34.0	20.0	34.0
4	32.301011992780936	35.0	33.0	37.0	22.0	37.0
5	32.48167519184038	35.0	33.0	37.0	25.0	37.0
6	32.43596817779926	36.0	33.0	37.0	23.0	37.0
7	32.315216140484125	35.0	33.0	37.0	22.0	37.0
8	32.23856101972201	35.0	33.0	37.0	21.0	37.0
9	33.48915317886371	38.0	33.0	39.0	18.0	39.0
10	33.36195164145555	38.0	33.0	39.0	17.0	39.0
11	33.40659756385249	38.0	34.0	39.0	17.0	39.0
12	33.32155727785162	38.0	33.0	39.0	17.0	39.0
13	33.26444964201942	38.0	33.0	39.0	17.0	39.0
14	34.088812843188585	39.0	33.0	41.0	17.0	41.0
15	34.28966651131479	39.0	34.0	41.0	17.0	41.0
16	34.22884289706308	39.0	34.0	41.0	17.0	41.0
17	34.00793972730952	39.0	33.0	41.0	15.0	41.0
18	33.99595413859305	39.0	33.0	41.0	15.0	41.0
19	34.130083218737354	39.0	34.0	41.0	16.0	41.0
20	34.12783253451784	39.0	34.0	41.0	11.0	41.0
21	34.02294508790806	39.0	34.0	41.0	10.0	41.0
22	34.02204222502239	39.0	34.0	41.0	10.0	41.0
23	33.69442526520099	39.0	33.0	41.0	2.0	41.0
24	33.77340299234557	39.0	34.0	41.0	2.0	41.0
25	33.4876878846637	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5580704002604371
1101	2	0.1961212403360797
1101	3	0.4803341918811981
1101	4	0.5838776614110515
1101	5	0.47040024045647044
1101	6	0.45226423515425296
1101	7	0.1264556541900106
1101	8	0.4757430106378493
1101	9	0.5772959460541216
1101	10	0.5661961932361876
1101	11	0.5636188284909878
1101	12	0.35862406306314654
1101	13	0.7390075296618335
1101	14	0.9659539493571572
1101	15	0.7707462693048512
1101	16	0.5666625237334912
1101	17	0.053099574403944416
1101	18	0.42299281496165264
1101	19	0.004525764652385078
1101	20	-0.6148237415670152
1101	21	0.4919238580862739
1101	22	0.5846332401952594
1101	23	0.13005873691826508
1101	24	0.6103850530323598
1101	25	0.24710925186945332
1102	1	0.7133953195784208
1102	2	0.7634067115669225
1102	3	0.6671713160259323
1102	4	0.7065038176812592
1102	5	0.6489434690322184
1102	6	0.5679387405751299
1102	7	0.5602712496791398
1102	8	0.6586081114305458
1102	9	0.7935354836680375
1102	10	0.7993789076241953
1102	11	0.641254495807992
1102	12	0.7235085463087856
1102	13	0.7912532534445162
1102	14	0.9076607576150124
1102	15	0.793456415578838
1102	16	0.7957730836699781
1102	17	1.0588428014781925
1102	18	1.0765349877971246
1102	19	0.8863538938900106
1102	20	1.1120318509941924
1102	21	1.1554759501881122
1102	22	0.8478652318469386
1102	23	0.8902426891030828
1102	24	0.25442134046773646
1102	25	0.6523981821746432
1103	1	0.7860987554081724
1103	2	0.8991918560664622
1103	3	1.0753855915148094
1103	4	1.2023872131872722
1103	5	1.1010668378650905
1103	6	0.6447483443847375
1103	7	0.5543013788794866
1103	8	0.6809658954538094
1103	9	0.8214937967209792
1103	10	1.1067034933730895
1103	11	0.7720644708988971
1103	12	0.9382679938939305
1103	13	1.0797066821164876
1103	14	1.1015717443089201
1103	15	1.1394947425225013
1103	16	1.1513925923780022
1103	17	0.7371044661298995
1103	18	1.0667717673659425
1103	19	0.97778256031593
1103	20	1.1392319004983662
1103	21	0.9402441810696658
1103	22	0.9988204499654643
1103	23	0.2807971820224182
1103	24	0.44288446678557847
1103	25	0.8353983936756819
1104	1	0.7192877198628516
1104	2	0.6894591222609172
1104	3	0.7359224614446234
1104	4	0.9075894913340576
1104	5	0.6319508620226202
1104	6	0.5927395293562583
1104	7	0.7976064926545163
1104	8	0.6196269476118488
1104	9	0.8685553681365263
1104	10	0.8791975466102144
1104	11	0.6029994358063746
1104	12	0.7955398043267152
1104	13	0.8604767695316937
1104	14	1.1478561568804793
1104	15	0.8713082332208515
1104	16	0.8536898511334883
1104	17	1.0580694147775063
1104	18	0.9651686774014934
1104	19	0.4129444681021468
1104	20	0.6483828585961078
1104	21	0.8066191696759972
1104	22	0.5971721160881742
1104	23	0.9469336752994124
1104	24	0.8224592107586943
1104	25	0.3523861781867552
1105	1	0.4594509715703303
1105	2	0.5863086141816218
1105	3	0.6718128495838869
1105	4	0.8001598728099921
1105	5	0.5275010036184895
1105	6	0.7618466424663666
1105	7	0.7789179202740399
1105	8	0.8645482369671669
1105	9	0.6516117566089576
1105	10	0.7466471551392857
1105	11	0.8210758142838088
1105	12	0.8894261944649813
1105	13	0.8855954601863658
1105	14	0.9843004347415487
1105	15	0.9441718014367382
1105	16	0.9009995296275761
1105	17	1.280741412112178
1105	18	1.0115767233785036
1105	19	0.7266875965379285
1105	20	0.8178397967936775
1105	21	0.7050033109386931
1105	22	0.732637716621241
1105	23	0.9677878315546238
1105	24	0.9690109348966232
1105	25	1.2432511623613323
1106	1	0.48403683814624543
1106	2	0.5730062163008078
1106	3	0.3935763543345807
1106	4	0.6255068618921342
1106	5	0.43103987835290525
1106	6	0.5301020852971021
1106	7	0.4816552019399509
1106	8	0.46076791853742805
1106	9	0.5764422861294705
1106	10	0.3847708846855511
1106	11	0.15210845747141377
1106	12	0.3719231361781752
1106	13	0.45979547674250654
1106	14	0.7183527337454194
1106	15	0.5564376010522736
1106	16	0.5127396718264094
1106	17	0.6315719784241125
1106	18	0.6273574833052891
1106	19	0.6341456773134908
1106	20	0.6563153442959972
1106	21	0.7121969533812802
1106	22	0.6740533584935591
1106	23	0.9791885729017409
1106	24	0.6891569968349813
1106	25	0.893599761068117
1107	1	0.3265970964457381
1107	2	0.389923710496376
1107	3	0.3598155807190473
1107	4	0.38895194206725847
1107	5	0.3764661812097714
1107	6	0.3468892358951834
1107	7	0.22033241488557564
1107	8	0.4138210591677449
1107	9	0.28183991874049497
1107	10	0.45976709821741224
1107	11	0.3527285473131698
1107	12	0.21230924901670534
1107	13	0.08731356715089333
1107	14	0.4668519559603226
1107	15	0.33242025635537686
1107	16	0.39296789339805116
1107	17	0.5127544790611296
1107	18	0.4452098342119868
1107	19	0.28986760978428094
1107	20	0.39734034567694465
1107	21	0.2503620576472443
1107	22	0.2792477691320201
1107	23	0.5746075701024225
1107	24	0.33216699845647923
1107	25	0.589787188568387
1108	1	0.85395560493426
1108	2	0.8385128302821769
1108	3	0.7298368428907125
1108	4	0.9079766479816271
1108	5	0.7684333659582379
1108	6	0.8412563777872748
1108	7	0.7036969862507689
1108	8	0.8247411517060286
1108	9	0.8458097371708249
1108	10	0.9804176593980571
1108	11	0.800282969692546
1108	12	0.7990256375248279
1108	13	0.9136935502102972
1108	14	1.0004402139974715
1108	15	0.913992253614893
1108	16	0.6625312563982462
1108	17	0.9614809480601707
1108	18	1.1207015657893535
1108	19	0.970390259054632
1108	20	1.0957758140531624
1108	21	0.9709643198272957
1108	22	1.0704853514367159
1108	23	1.3319095420605933
1108	24	1.13975187556359
1108	25	1.3690376503783739
1109	1	0.8280070319194976
1109	2	0.7861282947242536
1109	3	0.9045969917959518
1109	4	0.9589034751851173
1109	5	0.8562543473384352
1109	6	1.0152415834642596
1109	7	0.8254227177417306
1109	8	0.7968090878418224
1109	9	0.8952321547803095
1109	10	0.9884137162042563
1109	11	0.9170937892678168
1109	12	0.7625321170157235
1109	13	0.942089652983114
1109	14	1.1613406768644765
1109	15	0.7983024387004747
1109	16	0.6689018651917564
1109	17	1.1445979077290147
1109	18	1.261359490053934
1109	19	1.1044152954667368
1109	20	1.0418800473718548
1109	21	0.9592297151008182
1109	22	0.8710546168087276
1109	23	1.1612828026799207
1109	24	1.0332397287810693
1109	25	0.5972527902206011
1110	1	0.5375769832788038
1110	2	0.5477795927269256
1110	3	0.55917675626225
1110	4	0.37480492649176256
1110	5	0.465854885569982
1110	6	0.44807774314808313
1110	7	0.33794502846034646
1110	8	0.4623263769039383
1110	9	0.6082055370700274
1110	10	0.4886879718244046
1110	11	0.594104506500841
1110	12	0.4920228451580897
1110	13	0.5978346233472394
1110	14	0.5594627803194214
1110	15	0.5168451190410508
1110	16	0.6966503417837586
1110	17	0.7667373242059412
1110	18	0.10995847302521611
1110	19	0.5143682654328998
1110	20	0.6144974304731718
1110	21	0.6580213814522224
1110	22	0.6391669893420442
1110	23	0.6827444464203509
1110	24	0.6195871996502689
1110	25	0.7521811684980264
1111	1	0.1901927118113207
1111	2	0.16955654577138546
1111	3	0.2647888060500989
1111	4	0.1363366732986364
1111	5	0.21219643368123542
1111	6	0.18641746816463467
1111	7	0.014011484602981739
1111	8	0.33612533730588723
1111	9	0.35216325863571996
1111	10	0.22998015530586713
1111	11	-0.09225418738202507
1111	12	-0.42058902014993294
1111	13	-0.023187481676039567
1111	14	0.1601605047065675
1111	15	0.07590726703727313
1111	16	0.1551391264958042
1111	17	-0.3678049813577573
1111	18	-0.09065660440204937
1111	19	0.06473190771566806
1111	20	0.23610266535938962
1111	21	0.2009122453603709
1111	22	-0.13274736861392
1111	23	0.21771708914804577
1111	24	0.2705139611612708
1111	25	0.30128392375841173
1112	1	0.37226678588539386
1112	2	0.27904037881077315
1112	3	0.36199280369854137
1112	4	0.39441309893061316
1112	5	0.4120083090486659
1112	6	0.15029518715346057
1112	7	0.0956105439798236
1112	8	0.15455684818184778
1112	9	0.1238575561077937
1112	10	0.20551454684025572
1112	11	-0.027504040409660035
1112	12	0.154011802895333
1112	13	0.21179664530809106
1112	14	0.12565374397758688
1112	15	-0.018411369059137428
1112	16	0.2599598202053883
1112	17	0.4353155359849836
1112	18	0.174998892364556
1112	19	0.25311521165453144
1112	20	0.17187926735821435
1112	21	0.13434199256789725
1112	22	0.016094489116824207
1112	23	0.29210627198167316
1112	24	0.28306422571153433
1112	25	0.2355766927178493
1113	1	-0.16638788334252297
1113	2	-0.07582019402573792
1113	3	0.027809464561205033
1113	4	0.021371014282017597
1113	5	-0.10633421707871094
1113	6	-0.3319295813416545
1113	7	-0.3095014733048771
1113	8	-0.2217860299303176
1113	9	-0.2508733065919273
1113	10	-0.20947551146190335
1113	11	-0.21739421366854828
1113	12	-0.3180241295086148
1113	13	-0.19933468620540395
1113	14	0.13185841189491754
1113	15	-0.27175990513945436
1113	16	-0.3616134768810042
1113	17	-0.05093833491149269
1113	18	-0.29106640455707833
1113	19	-0.701415094609672
1113	20	-0.39367228156205414
1113	21	-0.07933336512727607
1113	22	-0.6345624867347723
1113	23	-4.3244886871469
1113	24	-1.8444085609969214
1113	25	-0.9854772395887395
1114	1	-0.8760740404901135
1114	2	-0.9532711142585875
1114	3	-0.8754329879982841
1114	4	-0.8986990792168861
1114	5	-0.8229181028205836
1114	6	-1.5496399071297375
1114	7	-1.3782385689187464
1114	8	-1.2170555661409104
1114	9	-1.461240295207137
1114	10	-1.1500532422535414
1114	11	-1.2968270839780445
1114	12	-2.2097099792593333
1114	13	-1.457576691695305
1114	14	-3.1351330084597784
1114	15	-1.9866823283384463
1114	16	-2.2291897110122214
1114	17	-2.0046347822569217
1114	18	-1.7897617210794223
1114	19	-1.9242982785399505
1114	20	-1.4827849390079848
1114	21	-1.3843277460761527
1114	22	-1.6734709824011205
1114	23	-5.5003398579724845
1114	24	-3.062303850870414
1114	25	-2.9096405035590784
1115	1	-1.4650592584290543
1115	2	-1.3160612914327885
1115	3	-0.9709505070643303
1115	4	-0.9528793393294741
1115	5	-1.0041973590459925
1115	6	-1.1653659969049492
1115	7	-1.6793668827960673
1115	8	-1.836442602617737
1115	9	-3.0759033639254163
1115	10	-3.611139103721257
1115	11	-2.448632891375901
1115	12	-2.6248060657759424
1115	13	-2.108038904079322
1115	14	-2.747100333066335
1115	15	-2.3425677495022974
1115	16	-2.404170041650719
1115	17	-2.3895065726387905
1115	18	-5.062276321894537
1115	19	-3.0125911188521073
1115	20	-3.2756399440629416
1115	21	-2.872211842061425
1115	22	-2.4121354624426274
1115	23	-3.057608746245478
1115	24	-2.790143489771328
1115	25	-2.9913141936319327
1116	1	-0.7153726102164129
1116	2	-0.5404546758771644
1116	3	-0.8503081450755623
1116	4	-0.39548170949670336
1116	5	-0.6208819639457062
1116	6	-2.7945328179734155
1116	7	-1.305774716407246
1116	8	-1.8016590447338778
1116	9	-2.614959717759639
1116	10	-2.123349316213254
1116	11	-1.716284746085801
1116	12	-1.338309654271022
1116	13	-2.7619112360310716
1116	14	-5.916370914510157
1116	15	-3.6210128521752516
1116	16	-2.5005129744150807
1116	17	-3.256879237328878
1116	18	-1.9902953288839953
1116	19	-1.810551641795307
1116	20	-2.548219712174923
1116	21	-3.251820883688584
1116	22	-2.8700052006809713
1116	23	-1.8241250521999497
1116	24	-2.7675401322218747
1116	25	-2.6519516329165107
1201	1	0.4728794803642593
1201	2	0.5301948495026245
1201	3	0.8733970946360827
1201	4	0.6526238824408637
1201	5	0.2725626735270339
1201	6	0.6579344721574714
1201	7	0.7603835071831853
1201	8	0.7021622366587721
1201	9	1.065198643462928
1201	10	0.9736249301922797
1201	11	1.1201332558232622
1201	12	0.8111055963996989
1201	13	1.0328711717729533
1201	14	1.1167627351898375
1201	15	1.0997172629067506
1201	16	0.990248585397083
1201	17	1.2884808509429462
1201	18	1.0487747600478983
1201	19	1.2973795739692875
1201	20	1.1715946108364648
1201	21	1.3904733082820329
1201	22	1.1453870111189701
1201	23	1.4535862892031233
1201	24	1.2304787140182185
1201	25	1.3003292218445992
1202	1	0.8413481276469916
1202	2	0.8208221149608974
1202	3	0.8633697027362608
1202	4	1.0787372862632907
1202	5	0.9299298165925975
1202	6	1.1162999907846043
1202	7	1.1317840792237703
1202	8	1.0947620318038531
1202	9	1.370554992627092
1202	10	1.1938942951834974
1202	11	1.263055280893667
1202	12	1.4316718954660175
1202	13	1.3640776368041685
1202	14	1.7665841939875762
1202	15	1.4664326363372098
1202	16	1.3721691775386304
1202	17	1.7293002479969388
1202	18	1.71539326658241
1202	19	1.6762831364619117
1202	20	1.5371082428870224
1202	21	1.6698249767714302
1202	22	1.595433455958748
1202	23	1.6331320684717596
1202	24	1.5949655268127145
1202	25	1.883399418017504
1203	1	1.1012557141155987
1203	2	1.1555159680160862
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2211	16	-0.3249259339901158
2211	17	0.09714327522453203
2211	18	-0.22421087230215875
2211	19	-0.27955191865415685
2211	20	-0.4148201769705864
2211	21	-0.10212377510656978
2211	22	-0.0272450135598703
2211	23	0.014291112393614469
2211	24	-0.2955214832492885
2211	25	0.16920273369368743
2212	1	-0.2052049750614593
2212	2	-0.16842905343657222
2212	3	-0.4687752099707829
2212	4	-0.3558217354138442
2212	5	-0.6131386097461515
2212	6	-0.6374686755732739
2212	7	-0.5234283245865221
2212	8	-0.4657876279071971
2212	9	-0.3851936559945841
2212	10	-0.6728903725731641
2212	11	-0.8472184104142926
2212	12	-0.4475425325931184
2212	13	-0.4864038985789634
2212	14	-0.3336118727741919
2212	15	-0.6971185193809788
2212	16	-0.7671505994391197
2212	17	-0.6680944372778299
2212	18	-0.35117683301462677
2212	19	-0.7066559686175253
2212	20	-0.3875247141464797
2212	21	-0.28673259510585325
2212	22	-0.3567683397673349
2212	23	-0.31283932145453264
2212	24	-0.3714099846557488
2212	25	-0.24853957653410674
2213	1	-0.21777738672758673
2213	2	-0.14465480319226032
2213	3	-0.5248163587477244
2213	4	-0.27926509360588625
2213	5	-0.18412024997489596
2213	6	-0.227681070741248
2213	7	-0.47260081577078594
2213	8	-0.24789233173930114
2213	9	-0.8519699387818775
2213	10	-0.3796997631446928
2213	11	-0.533803626729636
2213	12	-0.5562573857632529
2213	13	-0.6250753511969336
2213	14	-0.6113746634891513
2213	15	-0.48017428240207494
2213	16	-0.46759277972233804
2213	17	-0.4866143595432817
2213	18	-0.549834314221961
2213	19	-0.3477655438643481
2213	20	-0.343982961551923
2213	21	-0.5258454848468972
2213	22	-0.7079291559058021
2213	23	-0.52990506511631
2213	24	-0.31651831882513903
2213	25	-0.16688597090689683
2214	1	-2.8911767209125436
2214	2	-1.6363243731221928
2214	3	-1.7224231020605423
2214	4	-3.271631446720118
2214	5	-1.823473983571457
2214	6	-1.7118253822807503
2214	7	-1.4736701844077977
2214	8	-1.2907541751179252
2214	9	-1.0270134877308976
2214	10	-1.1436499431470182
2214	11	-1.2392443420937056
2214	12	-1.0453227285418691
2214	13	-1.5198639936310308
2214	14	-1.4591517315803557
2214	15	-1.8396989214296724
2214	16	-1.5442631238558135
2214	17	-3.646373195141159
2214	18	-3.3991704346880347
2214	19	-2.3229755465740602
2214	20	-2.1519901616448287
2214	21	-2.037490473814497
2214	22	-2.9835959387029973
2214	23	-1.7112032108988338
2214	24	-2.914772489846701
2214	25	-2.818462442861371
2215	1	-1.795238945755628
2215	2	-3.366792977970608
2215	3	-2.007184154785655
2215	4	-3.626754111168026
2215	5	-2.2479557797958023
2215	6	-2.2003441192044875
2215	7	-2.3174971778565414
2215	8	-1.7582430476713036
2215	9	-4.115057238185287
2215	10	-2.4920894752691893
2215	11	-2.027486407477042
2215	12	-2.020069887104956
2215	13	-2.033105655114312
2215	14	-1.7625572149275541
2215	15	-1.8659354614602108
2215	16	-2.0487607529624654
2215	17	-2.328889573288553
2215	18	-2.011999108361021
2215	19	-2.115916181343394
2215	20	-1.9815625122623928
2215	21	-2.615619083671273
2215	22	-2.388600260433627
2215	23	-2.3946863238373695
2215	24	-2.2429315941579304
2215	25	-3.1941050478551922
2216	1	-2.703627708325932
2216	2	-2.6406195766027594
2216	3	-3.9226795788836313
2216	4	-4.544682133343933
2216	5	-4.199306526138553
2216	6	-3.2352162475878963
2216	7	-4.628520863869959
2216	8	-2.7482202020099464
2216	9	-3.2608899133481444
2216	10	-2.346282299511042
2216	11	-2.551892722664128
2216	12	-2.8410566192908036
2216	13	-2.41856639099554
2216	14	-3.4438074349445387
2216	15	-2.8761857708379424
2216	16	-3.200880046850102
2216	17	-2.795134228568191
2216	18	-2.30827827133065
2216	19	-3.1827189802302662
2216	20	-3.573762492584976
2216	21	-3.2003111817442402
2216	22	-2.9352896704631526
2216	23	-2.9254070137384325
2216	24	-3.29043360026154
2216	25	-3.360128512723332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113389.0
3	2.0
4	1029.0
5	6179.0
6	7509.0
7	6825.0
8	6181.0
9	5606.0
10	4920.0
11	4751.0
12	4408.0
13	4269.0
14	4247.0
15	4217.0
16	4411.0
17	4658.0
18	5281.0
19	6485.0
20	8288.0
21	10761.0
22	13383.0
23	15825.0
24	18790.0
25	21462.0
26	23719.0
27	25881.0
28	28617.0
29	33802.0
30	40457.0
31	50692.0
32	62669.0
33	79907.0
34	102899.0
35	135366.0
36	185120.0
37	292099.0
38	741484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32280857561685	19.357054851098653	12.986008310437214	24.334128262847283
2	13.989920302295026	20.601425159922893	43.3384802769759	22.070174260806183
3	18.829112178953864	26.449871464062923	29.41480544104298	25.30621091594023
4	11.663197246714361	17.061106387712837	39.1905869625576	32.0851094030152
5	12.141191432184408	39.65801243826543	35.475287231476514	12.725508898073645
6	30.17674047208531	37.66998751861291	19.09791386643236	13.055358142869414
7	27.44480327845252	32.3343618486403	22.5066341636635	17.714200709243684
8	24.6951658516637	38.033327765586336	20.831581412940594	16.439924969809372
9	27.536725679854573	14.880210842737501	20.674499046946295	36.90856443046163
10	15.902799640638557	29.101653984074456	34.16054933157236	20.83499704371463
11	33.16690469609018	22.61826607082527	26.14186937559507	18.072959857489483
12	24.416001781559324	25.081590809611203	31.83687789407401	18.66552951475546
13	29.167600438228792	23.166908447517397	25.964719990901425	21.700771123352382
14	20.457807700226084	23.851917474062475	28.061381437470878	27.628893388240567
15	22.002159516268353	33.55427584221135	23.513389000863807	20.930175640656493
16	20.51256087518212	28.675402107332282	29.94660644254746	20.86543057493814
17	20.386938544348908	30.010678966276068	28.73203473903726	20.870347750337768
18	20.138911553916422	27.33877485980214	32.49019430914756	20.032119277133877
19	23.13861340520866	26.84449282206634	28.7208054490096	21.296088323715402
20	23.673283292699317	27.434105699641016	29.5341805686203	19.358430439039374
21	22.289143328790527	26.87568382056548	28.08107952473271	22.75409332591128
22	23.032341578676817	29.373226224022414	28.070165334039842	19.52426686326092
23	20.99337208018775	29.08437759465543	29.508977207826803	20.413273117330018
24	22.77811558612521	26.954402591936528	30.234828259249326	20.03265356268894
25	21.595247362058466	28.151912414630132	29.87598663356572	20.37685358974568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	394.0
1	394.0
2	903.5
3	1413.0
4	1413.0
5	1413.0
6	2783.5
7	4154.0
8	4154.0
9	4154.0
10	5057.5
11	5961.0
12	5961.0
13	5961.0
14	8488.5
15	11016.0
16	11016.0
17	11016.0
18	19608.5
19	28201.0
20	28201.0
21	28201.0
22	44820.0
23	61439.0
24	61439.0
25	61439.0
26	90867.5
27	120296.0
28	120296.0
29	120296.0
30	154576.0
31	188856.0
32	188856.0
33	188856.0
34	207364.5
35	225873.0
36	225873.0
37	225873.0
38	234882.5
39	243892.0
40	243892.0
41	243892.0
42	251688.5
43	259485.0
44	259485.0
45	259485.0
46	265838.0
47	272191.0
48	272191.0
49	272191.0
50	254739.5
51	237288.0
52	237288.0
53	237288.0
54	203227.5
55	169167.0
56	169167.0
57	169167.0
58	142465.5
59	115764.0
60	115764.0
61	115764.0
62	94057.5
63	72351.0
64	72351.0
65	72351.0
66	55206.0
67	38061.0
68	38061.0
69	38061.0
70	28015.5
71	17970.0
72	17970.0
73	17970.0
74	12754.0
75	7538.0
76	7538.0
77	7538.0
78	5235.0
79	2932.0
80	2932.0
81	2932.0
82	1946.0
83	960.0
84	960.0
85	960.0
86	615.0
87	270.0
88	270.0
89	270.0
90	173.5
91	77.0
92	77.0
93	77.0
94	52.5
95	28.0
96	28.0
97	28.0
98	19.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3354929161464297
2	0.009973206596892579
3	0.008582711446364287
4	0.010788324443753992
5	0.01193907905108775
6	0.08079256305655767
7	0.08045692629608532
8	0.10356791466003831
9	0.08438867120447567
10	0.09071782154481135
11	0.08688197285369882
12	0.0968551794505914
13	0.08366944957489207
14	0.08779298691783803
15	0.08573121824636505
16	0.08707376528825443
17	0.09843746703567531
18	0.10088282057625955
19	0.09493725510503513
20	0.09340291562859011
21	0.08189536955525253
22	0.10112256111945409
23	0.09484135888775731
24	0.09086166587072807
25	0.08923143017700523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2085588.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.32453073640803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1378049447431	46.94813141856275
2	12.679845019915895	15.044517112337816
3	3.8347950488875493	6.824922502366642
4	1.5908614296525996	3.7750843112316663
5	0.8455088754747039	2.507970863550243
6	0.4568040531196587	1.6259811653887777
7	0.2955825685649453	1.2274708021784273
8	0.19605771553567766	0.9304825577125001
9	0.1467243632592466	0.7833918598157765
>10	0.7241759850696684	7.650712409499355
>50	0.050231592168528356	2.013721127211776
>100	0.034378189982990005	4.08095076200801
>500	0.004143156122401023	1.6239542258431938
>1k	0.0025183890155770603	2.565573933361103
>5k	4.8743013204717296E-4	1.8856973229631417
>10k+	8.123835534119548E-5	0.5114376259688925
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10612	0.5088253288760771	No Hit
GTCCTACAGTGGACATTTCTAAATT	7629	0.3657961208062187	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6754	0.3238415257471754	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6650	0.3188549224487291	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6217	0.2980933914080825	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5998	0.287592755616162	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5879	0.2818869306881321	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3986	0.19112116103468182	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2902	0.13914541127010704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2887	0.13842618964052344	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2734	0.13109012901877073	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2381	0.11416444666923668	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2380	0.11411649856059777	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2363	0.11330138071373637	No Hit
GAATATGGCAAGAAAACTGAAAATC	2272	0.10893810282759586	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2269	0.10879425850167915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.794810863890663E-5	0.0
6	4.794810863890663E-5	0.0	0.0	4.794810863890663E-5	0.0
7	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
8	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
9	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
10	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
11	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
12	4.794810863890663E-5	0.0	0.0	9.589621727781326E-5	0.0
13	4.794810863890663E-5	0.0	0.0	1.438443259167199E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCCA	60	1.4695433E-6	15.831114	9
GGTATCA	1870	0.0	14.153969	1
GCACCGT	70	1.0924288E-4	12.212866	6
TAGGACC	1140	0.0	11.831958	4
TAAGACG	65	8.033266E-4	11.690948	4
AAATGTC	870	0.0	11.464185	7
CACCTTT	670	0.0	11.341965	14
AATGTCC	905	0.0	11.125779	8
TCGTACA	60	0.0058829337	11.0817795	9
GTATCAA	4460	0.0	11.057831	1
TAGAAAT	1030	0.0	10.974452	4
GTCGAGG	165	8.0035534E-11	10.938123	19
GGCGAGG	1750	0.0	10.910161	19
AGGACGG	105	3.472287E-6	10.855881	5
TGTAGGA	3905	0.0	10.824607	2
ATTTAGA	1030	0.0	10.814929	1
CAATAGT	150	2.1373125E-9	10.765415	4
GTCCTAC	3630	0.0	10.622419	1
CTGTAGG	3865	0.0	10.617023	1
CAAGTCG	215	0.0	10.603418	6
>>END_MODULE