FastQCFastQC Report
Thu 26 May 2016
SRR1512858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136660.655258852659298No Hit
TATCAACGCAGAGTACTTTTTTTTT101420.486289717815791No Hit
GGTATCAACGCAGAGTACTTTTTTT75430.3616725834632727No Hit
GTCCTACAGTGGACATTTCTAAATT71210.3414384816176541No Hit
CTGTAGGACGTGGAATATGGCAAGA63670.3052856077039185No Hit
GTCCTAAAGTGTGTATTTCTCATTT62390.2991482497981385No Hit
CTTTAGGACGTGAAATATGGCGAGG60210.2886955621148568No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46480.22286280895363803No Hit
GTCCTACAGTGTGCATTTCTCATTT36470.1748667522060925No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29040.13924130748738486No Hit
CTGAAGGACCTGGAATATGGCGAGA28590.13708364259863404No Hit
CTGTAGGACCTGGAATATGGCGAGA27920.13387111931982731No Hit
TTTCTAAATTTTCCACCTTTTTCAG26600.12754196897949163No Hit
ATTTAGAAATGTCCACTGTAGGACG23600.11315753638781964No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22750.10908194715351259No Hit
GTCCTTCAGTGTGCATTTCTCATTT22070.10582147576606693No Hit
GAATATGGCAAGAAAACTGAAAATC21890.1049584098105666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.015.27351951
GGACCGT707.187664E-613.58556656
TAGGACC10950.013.0275694
TACCGTA550.00305673312.0974027
CGAATCT550.003085430712.08231716
GGCGAGG16050.011.828176519
CACCTTT9500.011.59203614
TATGTCG1850.011.2892716
CCCGTAC1105.0165727E-711.21929516
CCACCTT9750.011.19689813
CAGTCCG600.00588586311.0810389
GGACGAG1553.074092E-1011.0437516
AGGACCT23200.010.9858345
ACCGTAT700.001491661810.8572398
CCGGTTT1053.4971126E-610.84942812
GTATCAA46050.010.8429431
GACGTGG15900.010.7604927
CCCTATA1158.697243E-710.7508342
ATAGGAC4250.010.7411053
AAATGTC7400.010.6611287
Produced by FastQC (version 0.11.5)