FastQCFastQC Report
Thu 26 May 2016
SRR1512857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236677
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69180.3092981239579966No Hit
CTGTAGGACGTGGAATATGGCAAGA58680.262353482420573No Hit
GTATCAACGCAGAGTACTTTTTTTT58640.26217464569090665No Hit
GTCCTAAAGTGTGTATTTCTCATTT58080.2596709314755774No Hit
CTTTAGGACGTGAAATATGGCGAGG48510.216884243902897No Hit
GTCCTACAGTGTGCATTTCTCATTT40920.18294997444870226No Hit
TATCAACGCAGAGTACTTTTTTTTT33420.1494180876362568No Hit
GGTATCAACGCAGAGTACTTTTTTT32880.14700379178576076No Hit
CTGTAGGACCTGGAATATGGCGAGA32360.14467891430009786No Hit
CTGAAGGACCTGGAATATGGCGAGA23890.10681023679324282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11650.013.1269164
TCGAACT2400.013.061254519
GGTATCA16050.012.93345451
GGACCGA550.003072392712.0892146
GGCGAGG14900.011.92168219
AATCCCG3300.011.80190419
AAGGCGT2350.011.7217616
TCCAACG1302.6211637E-911.69119318
GTATCAA36400.011.1178441
TAAGACG600.005883157311.08177954
CTCGAAC2750.011.05349218
AGGACCT22500.010.9762395
GCCTCGA2800.010.85586516
CACCTTT8150.010.83921414
ACCTTTT8300.010.75776915
TTAGGAC20500.010.65704253
AAATGTC8300.010.6430867
TGTAGGA33100.010.589152
GGACCTG22400.010.5587876
TGGCGAG33800.010.51083218
Produced by FastQC (version 0.11.5)