##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512857_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2236677
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.785015896349808	31.0	30.0	34.0	22.0	34.0
2	28.86767646826073	31.0	30.0	34.0	18.0	34.0
3	28.99251031776157	31.0	30.0	34.0	22.0	34.0
4	32.412647870032195	35.0	33.0	37.0	23.0	37.0
5	32.61074263293269	35.0	33.0	37.0	25.0	37.0
6	32.56377518971224	36.0	33.0	37.0	25.0	37.0
7	32.47103761517644	36.0	33.0	37.0	23.0	37.0
8	32.39245720325286	36.0	33.0	37.0	23.0	37.0
9	33.702527454791195	38.0	34.0	39.0	22.0	39.0
10	33.55273693966541	38.0	34.0	39.0	19.0	39.0
11	33.63656576251287	38.0	34.0	39.0	21.0	39.0
12	33.53546980632429	38.0	34.0	39.0	18.0	39.0
13	33.51041165085527	38.0	34.0	39.0	18.0	39.0
14	34.32960056369337	39.0	34.0	41.0	17.0	41.0
15	34.52690576243239	39.0	34.0	41.0	18.0	41.0
16	34.47167740357683	39.0	34.0	41.0	18.0	41.0
17	34.227733374108105	39.0	33.0	41.0	17.0	41.0
18	34.225603428657784	39.0	34.0	41.0	17.0	41.0
19	34.35430417534584	39.0	34.0	41.0	18.0	41.0
20	34.334282509276036	39.0	34.0	41.0	18.0	41.0
21	34.23313111370126	39.0	34.0	41.0	16.0	41.0
22	34.22899819687867	39.0	34.0	41.0	15.0	41.0
23	33.8990922694694	39.0	34.0	41.0	9.0	41.0
24	33.97046109026918	39.0	34.0	41.0	2.0	41.0
25	33.68264304591141	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7803678334087998
1101	2	0.4329523555007455
1101	3	0.7075448147298822
1101	4	0.850871939634608
1101	5	0.662432403703967
1101	6	0.7156574946499674
1101	7	0.5309255074829835
1101	8	0.6844308793379952
1101	9	0.7950232540126052
1101	10	0.8631223015094918
1101	11	0.9661200385884996
1101	12	0.7869857633682997
1101	13	1.2501584412458797
1101	14	1.2906598090843886
1101	15	1.1179542872079224
1101	16	0.9442765213413722
1101	17	0.4163040292560467
1101	18	0.878533856359752
1101	19	0.29738167882347
1101	20	-0.19623876442751254
1101	21	0.8658096547648455
1101	22	0.9780453572949597
1101	23	0.4816376142165737
1101	24	0.9720888602550346
1101	25	0.6186028848013692
1102	1	0.9485080059865219
1102	2	0.9786162772522466
1102	3	0.8277744734889829
1102	4	0.9940405223052693
1102	5	0.8841966537932109
1102	6	0.8430506652908676
1102	7	0.7785524966769657
1102	8	0.8701920258742746
1102	9	1.0403066326111414
1102	10	1.0200510493517072
1102	11	0.8913759937452284
1102	12	1.160487251751249
1102	13	0.9465293589822252
1102	14	1.2185310613808369
1102	15	1.1499813210156944
1102	16	1.1123868820622391
1102	17	1.288198060584321
1102	18	1.30995900264368
1102	19	1.2031631251543686
1102	20	1.2879046642289396
1102	21	1.450731978845205
1102	22	1.2035261233175518
1102	23	1.1935171105313813
1102	24	0.557294483637861
1102	25	0.9070143140779834
1103	1	0.6232241059295127
1103	2	0.7192924470489857
1103	3	0.8751296338030592
1103	4	0.9403607435322172
1103	5	0.7273551012222725
1103	6	0.3860165413983552
1103	7	0.350562525310842
1103	8	0.3345820596851041
1103	9	0.49014254792393785
1103	10	0.6850148483192271
1103	11	0.5940916526264388
1103	12	0.6902579180306248
1103	13	0.8736408042175299
1103	14	0.8114034293789558
1103	15	0.7890350828660928
1103	16	0.9256300966797895
1103	17	0.5938148050029071
1103	18	0.9924884718787013
1103	19	0.8646522240179664
1103	20	0.9637486843029421
1103	21	0.7357136076850281
1103	22	0.7608738595378455
1103	23	0.08051611178412799
1103	24	0.4032661221606233
1103	25	0.7330390339510444
1104	1	0.4831993376051642
1104	2	0.5141460767917962
1104	3	0.5007020638550124
1104	4	0.7316200403426478
1104	5	0.3997366252356045
1104	6	0.4065194344039469
1104	7	0.4978731931972291
1104	8	0.3379782072262074
1104	9	0.6361417328743926
1104	10	0.5696985122659939
1104	11	0.4188317525306289
1104	12	0.5958307425135345
1104	13	0.6451025766641649
1104	14	0.7462843330526141
1104	15	0.5971905733692751
1104	16	0.5916556244453872
1104	17	0.8632464704051799
1104	18	0.7450034946061663
1104	19	0.2011868603097895
1104	20	0.33451281507490904
1104	21	0.4615643702057426
1104	22	0.20927776395170383
1104	23	0.4500615478865555
1104	24	0.4655820914253965
1104	25	-0.012350448030169048
1105	1	0.6158806341672545
1105	2	0.6665711766067695
1105	3	0.7048719053701298
1105	4	0.8453179633418557
1105	5	0.6997807542162349
1105	6	0.7997204389235719
1105	7	0.8636125861355239
1105	8	0.9415070078851215
1105	9	0.6330992067784678
1105	10	0.6895746540206176
1105	11	0.782202906321821
1105	12	1.0387520939486308
1105	13	0.9298004610727162
1105	14	0.922997003333883
1105	15	0.9599030217946378
1105	16	0.9649144632799818
1105	17	1.2635362878487726
1105	18	1.004815411174782
1105	19	0.8338119662928065
1105	20	0.9696943502047617
1105	21	0.9095573250696916
1105	22	0.8466322436729641
1105	23	0.9590560772169852
1105	24	0.9903228195358196
1105	25	1.240285140402257
1106	1	0.6900710372270744
1106	2	0.699413916747762
1106	3	0.6810228708923312
1106	4	0.707961926747501
1106	5	0.6391676535274655
1106	6	0.7164689465517569
1106	7	0.6221928126552143
1106	8	0.7127007997390464
1106	9	0.8804841931986473
1106	10	0.6424418031340906
1106	11	0.4807749764028273
1106	12	0.6630145824267828
1106	13	0.6831499061381976
1106	14	0.9405144891393178
1106	15	0.5970259813649577
1106	16	0.7042443379212528
1106	17	1.0244130899213744
1106	18	0.9066683609442947
1106	19	0.9567788281206262
1106	20	0.995101002982345
1106	21	0.9634516725369977
1106	22	0.9556426443894424
1106	23	1.311898234036839
1106	24	0.8819000142941746
1106	25	1.1119021920860206
1107	1	0.899271818957132
1107	2	1.0021631421328685
1107	3	0.9088073153123091
1107	4	1.0550044044713403
1107	5	0.8481505359552131
1107	6	0.9986562902067675
1107	7	0.7170131782481661
1107	8	0.9598512077497787
1107	9	0.8921802155170155
1107	10	1.0665148646396787
1107	11	0.9996778632016117
1107	12	1.0854105858665264
1107	13	0.9831744932039328
1107	14	1.2135443536576531
1107	15	1.0260518483444443
1107	16	1.0104762824319593
1107	17	1.408088827706635
1107	18	1.2280297096692152
1107	19	1.066543644478081
1107	20	1.2802015265686038
1107	21	1.044102875040231
1107	22	1.008319311427158
1107	23	1.3877075378966808
1107	24	1.168979409313721
1107	25	1.537283464896845
1108	1	0.7537604100468904
1108	2	0.6793068288664585
1108	3	0.6456059112830346
1108	4	0.759489927861857
1108	5	0.5900567534586045
1108	6	0.7280265990205166
1108	7	0.6710312194460002
1108	8	0.7640935427026747
1108	9	0.8896266392245096
1108	10	0.879715819260781
1108	11	0.6027918319407775
1108	12	0.7631098974122921
1108	13	0.7780395945493837
1108	14	0.8567835157888837
1108	15	0.839557931572493
1108	16	0.5239081546937427
1108	17	0.906302410697279
1108	18	0.966552548006149
1108	19	0.881149175481184
1108	20	0.8986929141495423
1108	21	0.929507231016828
1108	22	0.9347207126427435
1108	23	1.1957614012242317
1108	24	1.0520495783420003
1108	25	1.2253505838114194
1109	1	0.5768854759936879
1109	2	0.5662995325328808
1109	3	0.7214549799661008
1109	4	0.7445071323184607
1109	5	0.48116951645047124
1109	6	0.6119174025893699
1109	7	0.5775383254971374
1109	8	0.49565319871629043
1109	9	0.48387011143043424
1109	10	0.5978110663888643
1109	11	0.4677768207708226
1109	12	0.4053767836447335
1109	13	0.6171875876525803
1109	14	0.6980851187850305
1109	15	0.6157902919164471
1109	16	0.18634877293975194
1109	17	0.6201038743900895
1109	18	0.791524875051671
1109	19	0.6389396703696377
1109	20	0.510490899408282
1109	21	0.5561372875799151
1109	22	0.6401250894944468
1109	23	0.7947285410480376
1109	24	0.6827305365434881
1109	25	0.3259475924845816
1110	1	0.5679875141190642
1110	2	0.6722981417603293
1110	3	0.5358063870637508
1110	4	0.6745008063439784
1110	5	0.5254190015820726
1110	6	0.565722248631161
1110	7	0.535999198638585
1110	8	0.5647796177438167
1110	9	0.6857072340695751
1110	10	0.6044845770378515
1110	11	0.536557116380628
1110	12	0.5990844011854222
1110	13	0.7187986705087184
1110	14	0.7345262636765284
1110	15	0.6764963365969763
1110	16	0.7588823492658534
1110	17	0.7538118215721283
1110	18	0.10442813630342584
1110	19	0.5745343433654213
1110	20	0.6537469082991834
1110	21	0.7656716927185485
1110	22	0.7917370356969755
1110	23	0.9513274899630417
1110	24	0.761747530339953
1110	25	0.9548037683937665
1111	1	0.4084652125207455
1111	2	0.4129867534938896
1111	3	0.5233357916108297
1111	4	0.45273986128369614
1111	5	0.3169478893031794
1111	6	0.29097997995106795
1111	7	0.1935736426478698
1111	8	0.3425314371695336
1111	9	0.4067679233195918
1111	10	0.40729563934450397
1111	11	0.10585052728330169
1111	12	-0.1526402111331322
1111	13	0.17154612108181766
1111	14	0.28157741209605547
1111	15	0.28620147932522144
1111	16	0.32964211442608615
1111	17	-0.15423761896686017
1111	18	0.19605041212241758
1111	19	0.22091569400395628
1111	20	0.4244772689123337
1111	21	0.3503287470831893
1111	22	0.060578564708634985
1111	23	0.3866198250744901
1111	24	0.3641647452304895
1111	25	0.5038235341984745
1112	1	0.04545604537900516
1112	2	0.05496460791527369
1112	3	-0.030844081238825538
1112	4	-0.07182224786378555
1112	5	-0.0038946517745870324
1112	6	-0.2466845382762486
1112	7	-0.4228497066668524
1112	8	-0.20158860131440548
1112	9	-0.28295638846463333
1112	10	-0.2853972039039263
1112	11	-0.3271715564637816
1112	12	-0.23532688977862648
1112	13	-0.2346724607750872
1112	14	-0.17657516708229792
1112	15	-0.4022084488389126
1112	16	-0.2084986611143549
1112	17	-0.010082522008850958
1112	18	-0.23317104242708808
1112	19	-0.03499725463012737
1112	20	-0.38771303244215005
1112	21	-0.249648189758382
1112	22	-0.3703679893587051
1112	23	-0.24402353811522204
1112	24	-0.1608079252359289
1112	25	-0.32946136444110863
1113	1	-0.17542868544395063
1113	2	0.025919375577611703
1113	3	0.007616330756064826
1113	4	0.03056667689245529
1113	5	-0.021488542524764398
1113	6	-0.36523096187688253
1113	7	-0.3525109130068387
1113	8	-0.22029973882727916
1113	9	-0.24197226263262195
1113	10	-0.026262967477386212
1113	11	-0.271256213175036
1113	12	-0.42179679112855695
1113	13	-0.3526466515630773
1113	14	0.13225943901141335
1113	15	-0.1327213349806584
1113	16	-0.2403456853763899
1113	17	-0.1072470168967925
1113	18	-0.2953561154118418
1113	19	-0.7550959811057041
1113	20	-0.4148110814704822
1113	21	-0.2665433038611269
1113	22	-0.6625158409468241
1113	23	-4.679880457945963
1113	24	-2.1737709483315335
1113	25	-1.1859584273507409
1114	1	-0.3824707254341888
1114	2	-0.626243334692024
1114	3	-0.5319007297826914
1114	4	-0.35320689441617503
1114	5	-0.305852497059
1114	6	-0.9981771536535327
1114	7	-0.8376349077611067
1114	8	-0.6541999665096192
1114	9	-0.8298716500182479
1114	10	-0.560788216829998
1114	11	-0.5090379182295237
1114	12	-1.4704318528488969
1114	13	-0.7451197753253211
1114	14	-2.4653550293207545
1114	15	-1.2673328235227785
1114	16	-1.3084790641238158
1114	17	-0.9395641418854908
1114	18	-1.012977667798836
1114	19	-1.213060409904493
1114	20	-0.7330209435353723
1114	21	-0.4264216511960086
1114	22	-0.6034073236966009
1114	23	-5.132716935253534
1114	24	-2.296805412054308
1114	25	-2.071144560551371
1115	1	-1.6193993945147298
1115	2	-1.517722214685591
1115	3	-1.2182027099347614
1115	4	-1.193558916867726
1115	5	-1.2423668340227643
1115	6	-1.358762887194633
1115	7	-1.9956877599425447
1115	8	-2.3034507104889492
1115	9	-3.5210733919113046
1115	10	-4.142338217019063
1115	11	-3.110841911259481
1115	12	-3.2344415585263064
1115	13	-2.5583935112479814
1115	14	-3.0268013450718705
1115	15	-2.8130436800005114
1115	16	-2.8954587297774275
1115	17	-2.7860638553627872
1115	18	-5.637259083630081
1115	19	-3.5338175104940746
1115	20	-3.744687849114193
1115	21	-3.3989847386148213
1115	22	-2.795891026965645
1115	23	-3.2421673204177424
1115	24	-3.014799175587651
1115	25	-3.2613336758510627
1116	1	-1.2545467411339999
1116	2	-1.1277533364808754
1116	3	-1.4211128981873493
1116	4	-1.3092011279981897
1116	5	-1.2442844101839121
1116	6	-3.829543626352489
1116	7	-2.201532185548821
1116	8	-2.4190106112330305
1116	9	-3.3112412795153716
1116	10	-2.661963598013376
1116	11	-2.1808440276862413
1116	12	-2.1057506368502423
1116	13	-3.3630761694200046
1116	14	-6.444920022533637
1116	15	-4.39902128963455
1116	16	-3.092406752400521
1116	17	-4.205148202159293
1116	18	-2.757966250712137
1116	19	-2.6120954819321085
1116	20	-3.324560198320551
1116	21	-4.096362619070323
1116	22	-3.713361234443397
1116	23	-2.7050207665542416
1116	24	-3.747159499581798
1116	25	-3.5441407829162337
1201	1	0.7267023217349013
1201	2	0.7292499129555488
1201	3	1.0480335496197348
1201	4	0.9806648201200829
1201	5	0.513310296944347
1201	6	0.9829228615711827
1201	7	1.1152432948725277
1201	8	0.9903906611283588
1201	9	1.4819346804667504
1201	10	1.3616214826717723
1201	11	1.4877287461261446
1201	12	1.3429357076397324
1201	13	1.4741268242852499
1201	14	1.6558438002227192
1201	15	1.679572727157229
1201	16	1.5444585567027573
1201	17	1.7467927198444855
1201	18	1.4662146419436226
1201	19	1.663598674598937
1201	20	1.6245578635229023
1201	21	1.8118540122897286
1201	22	1.6235599828842027
1201	23	2.071231265181744
1201	24	1.8274918384737688
1201	25	1.9716782582644257
1202	1	0.9183989249053113
1202	2	0.9268615374445943
1202	3	0.9231456586424187
1202	4	1.1557603478872807
1202	5	1.0008943439210825
1202	6	1.2358492507489345
1202	7	1.2704688109113889
1202	8	1.2435868541530652
1202	9	1.4739330017622265
1202	10	1.288425613512885
1202	11	1.3865309654841766
1202	12	1.5451332384664482
1202	13	1.4141611270738181
1202	14	1.9012791897498076
1202	15	1.608125396089946
1202	16	1.3781103580967624
1202	17	1.6714115997714032
1202	18	1.6097601563091857
1202	19	1.6759349262669758
1202	20	1.479871396846761
1202	21	1.4779339251698218
1202	22	1.504709743670496
1202	23	1.6341534188017661
1202	24	1.6215673204645498
1202	25	2.0578132418290807
1203	1	0.955512777627046
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2211	19	-0.0142440657254852
2211	20	0.01852695617851907
2211	21	0.058937441012979264
2211	22	-0.03168158484387362
2211	23	0.26561461699740363
2211	24	-0.14114109112561124
2211	25	0.35687074809128205
2212	1	0.21981871441727563
2212	2	0.4036638537384576
2212	3	-0.0061655536136129285
2212	4	0.3175681833320496
2212	5	0.1201918821557797
2212	6	0.04120528789557909
2212	7	0.14731955773401495
2212	8	0.20305931456290693
2212	9	0.3846813580755395
2212	10	0.02154249737298386
2212	11	-0.039438916351549835
2212	12	0.30669365636860846
2212	13	0.38017694106395794
2212	14	0.2728182750014341
2212	15	0.023115838336536854
2212	16	0.008649926836149291
2212	17	0.20972755485102113
2212	18	0.4432290362608242
2212	19	0.12794823501552344
2212	20	0.27783164097455426
2212	21	0.36727024870406666
2212	22	0.5151720949841092
2212	23	0.4923015119028378
2212	24	0.4316782806594617
2212	25	0.46914850355423
2213	1	-0.4222150095273811
2213	2	-0.22820969622075538
2213	3	-0.5477881273687331
2213	4	-0.32580624070429565
2213	5	-0.23943646971603272
2213	6	-0.13586114585677223
2213	7	-0.2936661328193395
2213	8	-0.1566968581204975
2213	9	-0.5938597404085755
2213	10	-0.3591131480110761
2213	11	-0.45284530126451017
2213	12	-0.47567199966259466
2213	13	-0.5498420156458792
2213	14	-0.6770709415583056
2213	15	-0.38598200448260656
2213	16	-0.35746300094614725
2213	17	-0.34947447368227813
2213	18	-0.43393873013493334
2213	19	-0.4233609191916585
2213	20	-0.23431214665841793
2213	21	-0.3415514298352633
2213	22	-0.4962730955815644
2213	23	-0.3095147107871199
2213	24	-0.11034380405339306
2213	25	0.053492002248951565
2214	1	-2.4753711412569217
2214	2	-1.211660195934364
2214	3	-1.568008863208398
2214	4	-3.0013383406791334
2214	5	-1.7219916603673475
2214	6	-1.3917656355172205
2214	7	-1.2289529529063792
2214	8	-1.0267840336546072
2214	9	-0.8157715932834648
2214	10	-0.8130739872729862
2214	11	-0.8577752557094698
2214	12	-0.799826742257892
2214	13	-1.1618670602105894
2214	14	-1.0311415866626064
2214	15	-1.4393556066495492
2214	16	-1.1656778296195895
2214	17	-3.5791212481607495
2214	18	-3.2958915076004445
2214	19	-1.9823512809018737
2214	20	-1.8109751598217372
2214	21	-1.8305698418862022
2214	22	-2.7982257793675025
2214	23	-1.6623685251516775
2214	24	-2.7643038403475373
2214	25	-2.6611904641468485
2215	1	-2.0348488467183756
2215	2	-3.551263152369394
2215	3	-2.1419780187244264
2215	4	-3.7696654754704717
2215	5	-2.2634228498136544
2215	6	-2.1437181762456845
2215	7	-2.1802627317801324
2215	8	-1.7894357968703005
2215	9	-4.296558134362282
2215	10	-2.517664950876764
2215	11	-2.1365250367392363
2215	12	-2.1449760462694236
2215	13	-2.1305438736210007
2215	14	-1.8781770665779831
2215	15	-2.023446576990871
2215	16	-2.164731541333488
2215	17	-2.4611852278942123
2215	18	-2.1701318493287616
2215	19	-2.184750857784003
2215	20	-2.028720507546467
2215	21	-2.6359440451714313
2215	22	-2.6566376051076226
2215	23	-2.66530710568556
2215	24	-2.4213991496682574
2215	25	-3.5492063917047574
2216	1	-2.7484303845813187
2216	2	-2.8975883148939126
2216	3	-4.073081807450656
2216	4	-4.676454623209722
2216	5	-4.466030537507184
2216	6	-3.46878261863662
2216	7	-5.129190313123452
2216	8	-2.9850091315005898
2216	9	-3.664428536255837
2216	10	-2.66488217442296
2216	11	-2.8492319413969227
2216	12	-3.213644480035615
2216	13	-2.777362689177963
2216	14	-3.6572093769390257
2216	15	-3.2260919574036286
2216	16	-3.435776642901182
2216	17	-3.3402491515036346
2216	18	-2.8637103069264747
2216	19	-3.5469726206684307
2216	20	-4.070193847040333
2216	21	-3.769126202329808
2216	22	-3.356254193146466
2216	23	-3.3988370085761446
2216	24	-3.871434815897203
2216	25	-3.75922535965379
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115670.0
3	4.0
4	1066.0
5	6214.0
6	7267.0
7	6591.0
8	5752.0
9	5460.0
10	4901.0
11	4609.0
12	4369.0
13	4197.0
14	4232.0
15	4394.0
16	4509.0
17	4945.0
18	5475.0
19	6619.0
20	8416.0
21	10918.0
22	13794.0
23	16632.0
24	19505.0
25	22357.0
26	24791.0
27	27281.0
28	30863.0
29	36455.0
30	43973.0
31	54233.0
32	67849.0
33	86992.0
34	113125.0
35	147283.0
36	201516.0
37	310235.0
38	804185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.382430980614686	18.534673984474704	12.463973551820892	24.618921483089718
2	14.417369935192282	20.074474773101443	42.918636668094514	22.58951862361176
3	19.426314572809947	25.815888936533053	28.725809718545204	26.031986772111797
4	11.804601041829685	16.42021954436719	38.63243086140982	33.1427485523933
5	12.504633506928686	39.41765145922492	35.20320582786739	12.874509205979
6	31.178077214611015	36.801735409166206	19.001978645661342	13.01820873056144
7	28.463081818865195	31.662571010269865	21.868209950565756	18.00613722029918
8	25.379744201065822	37.3648869707425	20.555082881557944	16.700285946633738
9	28.03202273863236	14.49941314317651	19.96915142248456	37.49941269570657
10	15.759844448869725	28.957346501777696	34.11792082375869	21.164888225593888
11	34.492114050262806	22.100124668081303	25.110684314106287	18.297076967549604
12	24.39780941047686	24.537037244228713	31.786551334166045	19.27860201112838
13	29.68714062876151	22.385164528822635	25.566022285603314	22.361672556812536
14	20.94203164448772	22.823535308063448	27.6460127569582	28.588420290490635
15	22.84220234145215	32.221569407459484	23.247568985939026	21.688659265149344
16	21.52425572664259	28.046341168731793	28.94389926299821	21.485503841627402
17	21.163983750789345	29.08272158614637	28.15475148884713	21.598543174217156
18	20.93770559302539	27.141412197512523	31.181620039473863	20.73926216998823
19	23.59483886674173	26.501064197323128	27.84200652659956	22.062090409335582
20	24.188505948763574	27.051188991945697	28.51311788960978	20.247187169680952
21	23.30375772371766	26.55280899680827	27.011500167573743	23.13193311190033
22	23.58909866385011	28.759020857901767	27.135725170683024	20.5161553075651
23	22.03080437116506	28.30029093066935	28.249945291071636	21.418959407093947
24	23.20071134277584	26.694775505930018	29.091664142032275	21.012849009261867
25	22.436031398191485	27.64695695211467	28.686448129931698	21.230563519762146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	289.0
1	289.0
2	694.0
3	1099.0
4	1099.0
5	1099.0
6	1811.0
7	2523.0
8	2523.0
9	2523.0
10	3004.5
11	3486.0
12	3486.0
13	3486.0
14	5281.0
15	7076.0
16	7076.0
17	7076.0
18	14274.5
19	21473.0
20	21473.0
21	21473.0
22	36974.5
23	52476.0
24	52476.0
25	52476.0
26	80846.0
27	109216.0
28	109216.0
29	109216.0
30	143709.0
31	178202.0
32	178202.0
33	178202.0
34	202615.5
35	227029.0
36	227029.0
37	227029.0
38	242233.5
39	257438.0
40	257438.0
41	257438.0
42	269096.5
43	280755.0
44	280755.0
45	280755.0
46	291851.0
47	302947.0
48	302947.0
49	302947.0
50	288825.5
51	274704.0
52	274704.0
53	274704.0
54	239078.5
55	203453.0
56	203453.0
57	203453.0
58	172600.0
59	141747.0
60	141747.0
61	141747.0
62	115385.5
63	89024.0
64	89024.0
65	89024.0
66	68125.0
67	47226.0
68	47226.0
69	47226.0
70	34572.0
71	21918.0
72	21918.0
73	21918.0
74	15557.5
75	9197.0
76	9197.0
77	9197.0
78	6382.0
79	3567.0
80	3567.0
81	3567.0
82	2411.0
83	1255.0
84	1255.0
85	1255.0
86	850.0
87	445.0
88	445.0
89	445.0
90	275.5
91	106.0
92	106.0
93	106.0
94	61.0
95	16.0
96	16.0
97	16.0
98	13.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.331384460071794
2	0.00961247421956769
3	0.008494744659152842
4	0.010148984408566816
5	0.011177295604148476
6	0.08114716608611793
7	0.07998472734328649
8	0.10479832358449612
9	0.08450035476736248
10	0.09129615049468474
11	0.08686994143544195
12	0.09880729314067252
13	0.08365088030144718
14	0.08830063527277296
15	0.08539453841569435
16	0.08704877816510832
17	0.09970147678900439
18	0.10224990018675026
19	0.09518584936492842
20	0.09366573716276422
21	0.08226489564653278
22	0.10157926245050133
23	0.09496230345284544
24	0.09075964030568562
25	0.09058080357601925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2236677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.02740212427566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15544451075372	44.77130004702744
2	13.195824675429588	15.314388496051842
3	4.401176456653471	7.661665082103769
4	1.9203118631046865	4.457228347375706
5	1.0452755221133123	3.0327311526165683
6	0.6010399674701277	2.0926072731090395
7	0.39064471628022485	1.5867668627521327
8	0.2425674104287094	1.126044533375275
9	0.18004198411131891	0.9402631750151956
>10	0.7787305238405974	7.847935886537409
>50	0.04728761771924139	1.8904798748585756
>100	0.03500023588760024	4.057828695630565
>500	0.003868904771755798	1.5880408342810837
>1k	0.0024760990539236132	2.53454334464759
>5k	3.0951238174045165E-4	1.0981763946178917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6918	0.3092981239579966	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5868	0.262353482420573	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5864	0.26217464569090665	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5808	0.2596709314755774	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4851	0.216884243902897	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4092	0.18294997444870226	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3342	0.1494180876362568	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3288	0.14700379178576076	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3236	0.14467891430009786	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2389	0.10681023679324282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.47091824165939E-5	0.0
4	0.0	0.0	0.0	4.47091824165939E-5	0.0
5	0.0	0.0	0.0	8.94183648331878E-5	0.0
6	0.0	0.0	0.0	8.94183648331878E-5	0.0
7	0.0	0.0	0.0	8.94183648331878E-5	0.0
8	0.0	0.0	0.0	8.94183648331878E-5	0.0
9	0.0	0.0	0.0	2.682550944995634E-4	0.0
10	0.0	0.0	0.0	5.365101889991268E-4	0.0
11	0.0	0.0	0.0	5.812193714157208E-4	0.0
12	0.0	0.0	0.0	5.812193714157208E-4	1.788367296663756E-4
13	0.0	0.0	0.0	5.812193714157208E-4	1.788367296663756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1165	0.0	13.126916	4
TCGAACT	240	0.0	13.0612545	19
GGTATCA	1605	0.0	12.9334545	1
GGACCGA	55	0.0030723927	12.089214	6
GGCGAGG	1490	0.0	11.921682	19
AATCCCG	330	0.0	11.801904	19
AAGGCGT	235	0.0	11.721761	6
TCCAACG	130	2.6211637E-9	11.691193	18
GTATCAA	3640	0.0	11.117844	1
TAAGACG	60	0.0058831573	11.0817795	4
CTCGAAC	275	0.0	11.053492	18
AGGACCT	2250	0.0	10.976239	5
GCCTCGA	280	0.0	10.855865	16
CACCTTT	815	0.0	10.839214	14
ACCTTTT	830	0.0	10.757769	15
TTAGGAC	2050	0.0	10.6570425	3
AAATGTC	830	0.0	10.643086	7
TGTAGGA	3310	0.0	10.58915	2
GGACCTG	2240	0.0	10.558787	6
TGGCGAG	3380	0.0	10.510832	18
>>END_MODULE