FastQCFastQC Report
Thu 26 May 2016
SRR1512857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236677
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76330.34126518938586126No Hit
GTCCTACAGTGGACATTTCTAAATT66300.29642187942201753No Hit
CTGTAGGACGTGGAATATGGCAAGA55290.24719706958134768No Hit
GTCCTAAAGTGTGTATTTCTCATTT54140.24205551360343938No Hit
TATCAACGCAGAGTACTTTTTTTTT53830.240669528948525No Hit
CTTTAGGACGTGAAATATGGCGAGG51330.2294922333443765No Hit
GGTATCAACGCAGAGTACTTTTTTT43520.19457436187701665No Hit
GTCCTACAGTGTGCATTTCTCATTT37850.16922425544680791No Hit
CTGTAGGACCTGGAATATGGCGAGA30610.13685480737719394No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25900.1157967824589782No Hit
CTGAAGGACCTGGAATATGGCGAGA23860.10667610924599306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.014.0723771
CTGTGCG1155.344191E-912.3887659
CGGCAAA550.003089366512.08032417
CCCGTAC1103.8635335E-812.08032416
CCGTACA1103.8635335E-812.08032417
CACCTTT9650.011.70503114
ACCGTCC658.017034E-411.6938368
GGCGAGG14500.011.586419
CCCCGGT1406.91216E-1011.52561619
TAGGACC12150.011.5079494
TGTGCGA752.0798379E-411.39689910
TCCAACG1751.8189894E-1211.3900218
ACCTTTT10500.011.29987615
CCACCTT9700.011.25327813
TTCCACC9600.011.17350211
AGACGAA700.001495234610.85418910
TATGTCG1752.1827873E-1110.84763816
AATGTCC7950.010.636618
GACGTGG16100.010.6279767
GATATAC5550.010.625891
Produced by FastQC (version 0.11.5)